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Citations for Thermo Fisher :
701 - 750 of 10000+ citations for Respiratory syncytial virus RSV fusion protein exodomain human heterodimeric IgG1 Fc tag recombinant antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... and the GST-fusion protein was purified employing standard procedures using glutathione Sepharose beads (Thermo Scientific).
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bioRxiv - Genetics 2019Quote: ... The His-ChpBK fusion protein was purified from clarified lysates using a Ni-NTA beads (Invitrogen). Protein purity was determined by SDS-PAGE analysis and samples were quantified using the Bio-Rad protein assay and stored at −80 □.
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bioRxiv - Bioengineering 2021Quote: Both Nefmut/SARS-CoV-2-based fusion proteins were cloned into the pVAX1 plasmid (Thermo Fisher) as already described (22) ...
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bioRxiv - Biophysics 2020Quote: ... Fusion protein expression plasmids were transfected into (70-90% confluent) cells using Turbofect™ (Thermo Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... the designed fusion protein-encoding sequences were produced using Phusion Site-Directed Mutagenesis (Thermo Scientific, Poland), via sequence exchange of the previously prepared CleavEx construct (HKU1-S primers ...
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bioRxiv - Microbiology 2020Quote: ... constructs encoding variants of BteA-GFP fusion protein (S3 Table) using Lipofectamine 2000 reagent (Invitrogen, USA). Eighteen h after transfection ...
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bioRxiv - Evolutionary Biology 2021Quote: The Rtl6-Venus fusion protein construct (pRtl6CV) was generated by Gateway cloning technology (Thermo Fisher Scientific) and the Kuroyanagi et al ...
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bioRxiv - Microbiology 2020Quote: ... GST fusion proteins were purified on glutathione superflow agarose according to the manufacturer’s protocol (Thermo Scientific). The GST tag of N-terminal GST-6xHis-RhoGAP domain was removed by addition of PreScission protease (GE healthcare Lifesciences ...
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bioRxiv - Cancer Biology 2022Quote: Protein data were generated through shotgun proteomics as previously described using an Orbitrap Fusion (Thermo Scientific) tribid mass spectrometer.3 Peptides were detected using a Top25 data-dependent acquisition method ...
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bioRxiv - Plant Biology 2023Quote: ... Flag-fusion protein purification was performed using Pierce™ Anti-DYKDDDDK Magnetic Agarose (Thermo Scientific, A36797) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... with 1.3 µg monoclonal antibody detecting the Twin-Strep-tag and 0.325 µg goat anti-human Alexa Fluor 488 (A11013, Invitrogen). Biotinylated lectins were precomplexed (on ice ...
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bioRxiv - Molecular Biology 2023Quote: ... Precomplexation of HAs was performed with 1.3 µg monoclonal antibody detecting the Twin-Strep-tag and 0.325 µg goat anti-human Alexa Fluor 488 (A11013, Invitrogen) and incubated on ice for 20 min ...
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bioRxiv - Neuroscience 2020Quote: ... which was generating by replacing a V5 tag with a 3HA tag or a 3flag tag from pcDNA3.1-nV5-DEST vector (Invitrogen), and E ...
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bioRxiv - Microbiology 2019Quote: ... The purified virus was quantified using the bicinchoninic acid (BCA) protein assay kit (ThermoFisher Scientific, Waltham, MA, USA) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... cleared lysates were mixed with Protein G/A (50:50) recombinant protein G agarose beads (catalog #15920-010, Invitrogen, Carlsbad, CA) coupled with anti-HA (catalog #3724S ...
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bioRxiv - Microbiology 2019Quote: ... The cell lysate was incubated with superparamagnetic beads covalently linked to recombinant protein G (Dynabeads protein G Novex, Life technologies, USA) to obtain the precleared lysate ...
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bioRxiv - Cell Biology 2024Quote: SK-Mel28 cells were grown on coverslips and transiently transfected with ribonucleprotein complexes consisting of purified recombinant Cas9 protein (Truecut Cas9 Protein v2, Invitrogen, #36499) and synthetic guide RNAs (Invitrogen TrueGuide Synthetic sgRNA #35514 or Negative Control ...
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bioRxiv - Microbiology 2021Quote: ... and RSV-GFP-infected ovine PCLS was assessed using an Evos FL Auto 2 cell imaging system (ThermoFisher) or a Leica TCS-SL ...
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bioRxiv - Cancer Biology 2021Quote: ... along with 0.5μg pCMV-VSV-G-RSV-Rev and 1μg pCAG-HIVgp for viral particle preparation using Lipofectamine 2000 (Invitrogen)-mediated transfection ...
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bioRxiv - Immunology 2020Quote: ELISA of SARS-CoV-2 antigens was performed by coating antigens on MaxiSorp 96-well plates (ThermoFisher) at 2 µg/mL in 1X PBS [pH 7.4] overnight at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: Plasmids encoding for recombinant His-tagged proteins were transformed into Escherichia coli BL21(DE3) (Invitrogen). Bacterial cultures were grown at 37°C in LB media to OD600 of 0.6-0.8 and induced with 0.5 mM IPTG at 18 °C for 16h (overnight) ...
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bioRxiv - Immunology 2019Quote: ... The protein was expressed in Sf9 insect cells using a recombinant baculovirus system (Life Technologies). The protein was purified on a cobalt resin column and further purified by gel filtration to ensure the removal of monomeric and aggregated protein.
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bioRxiv - Biochemistry 2020Quote: ... The recombinant PfAdoMetDC protein was purified using the Strep-Tactin Sepharose column (Invitrogen, California, USA) as described previously (29 ...
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bioRxiv - Immunology 2021Quote: ... Concentration of purified recombinant Abs were determined by PierceTM BCA Protein Assay Kit (ThermoFisher Scientific) and the purity was assessed by sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE).
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bioRxiv - Immunology 2020Quote: Recombinant soluble ZIKV E protein was biotinylated and coupled to AF488 Neutravidin beads (Life Technologies). Microscale-purified mAbs were tested at a single 1:10 dilution ...
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bioRxiv - Immunology 2020Quote: ... Recombinant SARS-CoV-2 RBD protein was coupled to fluorescent beads (Thermo Scientific, Cat # F8819) by carbodiimide coupling ...
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bioRxiv - Cell Biology 2021Quote: Binding of recombinant proteins to pre-spotted PIP and membrane lipid strips (Thermo Fisher; P23751) was performed according to (Dowler et al. ...
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bioRxiv - Immunology 2021Quote: For competitive ELISA recombinant hACE2 protein were immobilized (400 ng per well) on immunoplates (Nunc MaxiSorp ...
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bioRxiv - Immunology 2020Quote: ... 100uL of recombinant protein at 0.5 ug/ml was coated on Maxisorp™ plates (Nunc) and incubated overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: Recombinant soluble ZIKV E protein was biotinylated and coupled to AF488 Neutravidin beads (Life Technologies). Microscale-purified mAbs were tested at a single 1:10 dilution ...
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bioRxiv - Cancer Biology 2022Quote: ... Integrin-β2 recombinant protein complexes were immobilized on Maxisorp Immuno plates (ThermoFisher, 12-565-135) and used for positive binding selections with library phage pools that were first exposed to neutravidin coated wells to deplete nonspecific binders ...
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bioRxiv - Molecular Biology 2022Quote: ... Recombinant protein concentration was determined by Pierce Detergent Compatible Bradford Assay Kit (Thermo Fisher # 23246) with BSA standards diluted in Protein Storage Buffer before aliquoting in single use volumes ...
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bioRxiv - Bioengineering 2023Quote: ... Hu5F9 and TJC4 were then purified using recombinant Protein A-Sepharose 4B (Thermo Fisher Scientific) buffer exchanged into PBS and concentrated using Amicon Centrifugal Filters (Millipore Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... Recombinant protein concentration was determined by Pierce Detergent Compatible Bradford Assay Kit (Thermo Fisher # 23246) with BSA standards diluted in Protein Storage Buffer before aliquoting in single use volumes ...
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bioRxiv - Biochemistry 2023Quote: ... Recombinant protein concentration was determined by Pierce Detergent Compatible Bradford Assay Kit (Thermo Fisher # 23246) with BSA standards diluted in Protein Storage Buffer before aliquoting in single use volumes ...
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bioRxiv - Biochemistry 2023Quote: ... Recombinant protein concentration was determined by Pierce Detergent Compatible Bradford Assay Kit (Thermo Fisher # 23246) with BSA standards diluted in Protein Storage Buffer before aliquoting in single use volumes ...
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bioRxiv - Biochemistry 2023Quote: ... Recombinant protein concentration was determined by Pierce Detergent Compatible Bradford Assay Kit (Thermo Fisher # 23246) with BSA standards diluted in Protein Storage Buffer before aliquoting in single use volumes ...
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bioRxiv - Cell Biology 2023Quote: Recombinant ectodomain proteins were generated by transfection of HEK293T cell cultures using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... then recombinant protein purified from the soluble fraction using HisPur Ni-NTA columns (Thermo Scientific). Purified proteins were dialyzed against EMSA buffer ...
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bioRxiv - Immunology 2023Quote: ... ELISAs were conducted with recombinant full proteins coated overnight in Nunc MaxiSorp 384 plates (ThermoFisher) (2µg/ml ...
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bioRxiv - Immunology 2023Quote: ... the recombinant proteins were purified using a CaptureSelectTM C-tagXL Affinity Matrix prepacked column (ThermoFisher), followed the manufacturer’s guidelines as previously detailed (56 ...
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bioRxiv - Genetics 2023Quote: ... Protein concentration of recombinant SIRT5 was subsequently determined by Bradford assay (#23200, Thermo Fisher Scientific).
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bioRxiv - Immunology 2024Quote: ... recombinant spike proteins were biotinylated with EZ-Link™ NHS-PEG4-Biotin (ThermoFisher, Cat. # A39259) for 2 h at 4°C and processed through Zeba spin desalting columns (ThermoFisher ...
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bioRxiv - Microbiology 2024Quote: Recombinant spike proteins were biotinylated with EZ-Link™ NHS-PEG4-Biotin (ThermoFisher, Cat. # A39259) for 2 h at 4°C and processed through Zeba spin desalting columns (ThermoFisher ...
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bioRxiv - Immunology 2024Quote: The recombinant proteins were purified using a CaptureSelectTM C-tagXL Affinity Matrix prepacked column (ThermoFisher), followed the manufacturer’s guidelines as reported previously (25 ...
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bioRxiv - Biochemistry 2021Quote: ... The peptide–mRNA fusions were then incubated with human and yeast DUBm-immobilized on Dynabeads M-280 streptavidin (Invitrogen) for 30 min at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: ... Induced pluripotent stem cells were derived from human fibroblasts using Sendai virus-based reprogramming kit (CytoTune-iPS Sendai Reprogramming Kit; cat #. A13780-01; Thermo Fisher), according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Mitochondrial respiratory supercomplexes were resolved with BN-PAGE using the 4–16% NativePAGE Novex Bis-Tris Mini Gels (Invitrogen) in a Bis-Tris/Tricine buffering system with cathode buffer initially supplemented with 0.02% G-250 followed by the 0.002% G-250 ...
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bioRxiv - Immunology 2022Quote: BN-PAGE analysis of native respiratory complexes was performed as previously described(48) using commercially available reagents (Thermo Fisher). In summary ...
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bioRxiv - Neuroscience 2021Quote: ... Loss of virus was monitored by staining with anti-Sendai virus antibody (Invitrogen, cat #14649482).