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Citations for Thermo Fisher :
651 - 700 of 10000+ citations for Respiratory syncytial virus RSV fusion protein exodomain human heterodimeric IgG1 Fc tag recombinant antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... LAM antigen was diluted to the desired concentration in control human serum in high-recovery glass vials (Thermo Scientific, Waltham, MA) C5000-995 and incubated overnight (18−24 hrs ...
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bioRxiv - Neuroscience 2023Quote: Low-passage-number Human embryonic kidney cells transformed with SV40 T-antigen (HEK293T) cells were cultured in DMEM (Thermo Fisher Scientific) with 10% fetal bovine serum at 37°C in a humidified atmosphere with 5% CO2 ...
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bioRxiv - Plant Biology 2021Quote: ... Respiratory rates were normalized to cells number obtained by Countless®II FL automated cell counter (Thermo Fisher). To discriminate between the individual contributions of the alternative and the cytochrome pathway dark respiration measurements were conducted as follows ...
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bioRxiv - Microbiology 2022Quote: The Spike gene sequence from the severe acute respiratory syndrome coronavirus 2 isolate Wuhan-Hu-1 (NC_045512.2) was codon optimized (GeneArt, ThermoFisher) and gene blocks with overlapping sequences were synthesized by Bio Basic Inc (Markham ...
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bioRxiv - Microbiology 2023Quote: ... were transfected with each plasmid expressing SPΔC of SARS-CoV-2 Delta variant (1.2 µg DNA) or human influenza A virus M2 (5 µg DNA) using Lipofectamine 3000 (Invitrogen, USA) to produce 293TN-S target cells ...
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bioRxiv - Molecular Biology 2023Quote: ... The Fc-tagged ACE2 or mAb were first immobilized on Protein G Dynabeads (ThermoFisher Scientific). The S protein solutions were then incubated with on-bead receptor or antibody at room temperature for 60 min ...
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bioRxiv - Microbiology 2024Quote: Intact VHH-Fc proteins were separated on an Ultimate 3000 HPLC system (Thermo Fisher Scientific) online connected to an LTQ Orbitrap Elite mass spectrometer (Thermo Fischer Scientific) ...
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bioRxiv - Immunology 2020Quote: ... or with two administrations two weeks apart of 2.5μg Spike protein (2019-nCoV Spike Protein-S1+S2 ECD, His tag, SinoBiological) formulated in final 20mg/ml Imject™ Alum Adjuvant (Thermo Scientific) via bilateral intramuscular injections in the quadriceps (50μl per side) ...
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bioRxiv - Developmental Biology 2021Quote: ... were coated with 10 μg/ml recombinant human laminin 521 (BioLamina) diluted in DPBS (Thermo Fisher Scientific) for 3 hours at 37°C and washed with DPBS twice ...
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bioRxiv - Neuroscience 2021Quote: ... Human recombinant full length wild type Flag-LRRK2 (#A15198) and Flag-LRRK2[G2019S] (#A15201) were from ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... and 5 μg/mL human recombinant laminin 521 (BioLamina #LN521-02) in E8 basal medium (Gibco #A1517001) supplemented with 1% Penicillin/Streptomycin (Pen/Strep ...
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bioRxiv - Cell Biology 2021Quote: ... anti-rabbit IgG antibodies (1:500 for IF) and recombinant human BMP2 (#PHC7145) were from Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... and 5 μg/mL human recombinant laminin 521 (BioLamina #LN521-02) in E8 basal medium (Gibco #A1517001) supplemented with 1% Penicillin/Streptomycin (Pen/Strep ...
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bioRxiv - Immunology 2020Quote: ... M0 cells were treated for two days with 20 ng/ml recombinant human IFN-y (Life Technologies) and 50 ng/ml Escherichia coli LPS (SigmaAldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... The medium was supplemented with 15% fetal bovine serum (FBS) and recombinant human insulin (Gibco 12585-014) for pancreatic cancer cells and with 10% FBS for both human lung and colon cancer cells ...
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bioRxiv - Neuroscience 2024Quote: ... hiPS cells were cultured on six-well plates coated with recombinant human vitronectin (VTN–N, Gibco, A14700) in Essential 8 medium (Gibco ...
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bioRxiv - Systems Biology 2021Quote: ... The protein digests were labeled with 10-plex Tandem Mass Tag (TMT) reagents (Thermo Fisher Scientific) (Dayon et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... Then Flag tag was captured with Pierce Protein A/G Agarose (20 μL per sample, ThermoFisher) at room temperature for 2 h with mixing constantly ...
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bioRxiv - Microbiology 2022Quote: ... The protein with a C-terminal Strep-tag was expressed in Expi293 cells (Thermo Fisher Scientific) and subsequently purified using Strep-Tactin XT Superflow high capacity resin (IBA Lifesciences ...
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bioRxiv - Microbiology 2020Quote: ... Bound protein was detected via the C-terminal 6XHis Tag using 6XHis Antibody MA1-135 (Invitrogen) followed by three washes in TBS-T and incubation with donkey anti-mouse horseradish peroxidase (HRP)-coupled secondary antibody (Dianova) ...
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bioRxiv - Neuroscience 2024Quote: ... extracted protein was immunoprecipitated using 25μL prewashed HA Ab-Tag Magnetic beads (Thermo Fisher Scientific, Massachusetts) with gentle rotation at 4°C for 30min ...
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bioRxiv - Immunology 2024Quote: ... the lysate supernatant containing His-tagged proteins was affinity-purified with His-Tag magnetic beads (Invitrogen) and used for pull-down assays ...
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bioRxiv - Immunology 2023Quote: ... or control antigen PE (Thermo Fisher) were mixed with adjuvants per manufacturer’s protocols and added to cells immediately after plating ...
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bioRxiv - Immunology 2023Quote: ... Monomeric antigens were biotinylated (ThermoFisher; A39257) at an optimized ratio which resulted in <1 biotin per molecule ...
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bioRxiv - Cell Biology 2023Quote: ... BacMam 2.0 virus (Invitrogen, C10593).
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bioRxiv - Cancer Biology 2021Quote: Cells (1-1.5×10^6) were incubated with FACS sample buffer (DPBS, 0.5% FBS) and human FC Block (Invitrogen) for 20 minutes on ice followed by incubation at 4°C for 30 minutes with the following - antibodies ...
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bioRxiv - Biochemistry 2021Quote: ... labeled with 50 µL Goat anti-Human IgG Fc PE conjugate (ThermoFisher Scientific Invitrogen Catalog # 12-4998-82) diluted in 1.95 mL TBSF for 10 minutes covered on ice ...
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bioRxiv - Immunology 2020Quote: ... and F(ab’)2-Goat anti-Human IgG-Fc secondary antibody conjugated with R-phycoerythrin (ThermoFisher Catalog # H10104). Labeled cells were acquired using a Thermo-Fisher Attune NxT flow cytometer and analyzed using Flowjo Software.
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bioRxiv - Cell Biology 2023Quote: CEM were counted and resuspended at a concentration of 2-3 x 106 cells/mL in ice cold FACS buffer (PBS, 10% FCS, 2mM EDTA) containing 1.25 µL anti-human CD71 (0.625 µg/mL, OKT9, Invitrogen) for 1 h at 4°C then moved to 37°C and incubated for 1 h with or without 100 µM cytochalasin D (Merck) ...
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bioRxiv - Biophysics 2019Quote: ... secondary antibodies: Alexa mouse IgG1 568 (ThermoFisher A21124) diluted at 1/800 and Alexa Fluor anti-rabbit 488 (Life Technologies A21206 ...
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bioRxiv - Neuroscience 2020Quote: ... anti-mouse-IgG1 Alexa 488 (A21121, Life Technologies), anti‐rabbit Alexa 568 (A11036 ...
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bioRxiv - Neuroscience 2020Quote: ... IgG1 (Jackson ImmunoResearch; Molecular Probes, Thermo Fisher Scientific), were used ...
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bioRxiv - Neuroscience 2020Quote: ... IgG1 (Jackson ImmunoResearch; Molecular Probes, Thermo Fisher Scientific), were used ...
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bioRxiv - Cell Biology 2021Quote: ... Rat IgG1 isotype control (Thermo Fisher MA1-90035) was used for IgG control ...
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bioRxiv - Neuroscience 2021Quote: ... Alexa 647 anti-mouse IgG1 (Invitrogen, 1:1000) was added for ~1 hour at room temperature and coverslips were mounted in Mowiol with DAPI (2.5 μg/ml) ...
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bioRxiv - Immunology 2022Quote: ... goat anti-mouse IgG1-AF488 (1:500, Invitrogen).
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bioRxiv - Molecular Biology 2019Quote: ... AF568 Gt anti-Ms IgG1 (1:100; Invitrogen), DL405 Gt anti-Ms IgG2b (1:50 ...
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bioRxiv - Molecular Biology 2019Quote: ... AlexaFluor568-goat α-mouse IgG1 (Invitrogen A-21124), and AlexaFluor647-goat α-mouse IgG2b (Jackson 15-605-207)) ...
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bioRxiv - Developmental Biology 2020Quote: ... Alexa fluor 488 (Invitrogen, goat anti chicken, IgG1) and Alexa fluor 555 (Invitrogen ...
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bioRxiv - Developmental Biology 2020Quote: ... goat anti-mouse Igg1 647 (Invitrogen, 1:200), goat anti-mouse IgG1 488 (Invitrogen ...
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bioRxiv - Developmental Biology 2020Quote: ... goat anti-mouse IgG1 488 (Invitrogen, 1:200), goat anti-mouse IgG1 594 (Invitrogen ...
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bioRxiv - Developmental Biology 2020Quote: ... goat anti-mouse IgG1 594 (Invitrogen, 1:200), goat anti-mouse IgG2b Alexa 647 (Invitrogen ...
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bioRxiv - Physiology 2020Quote: ... goat anti-mouse IgG1-Alexa546 (1:500, Invitrogen); membrane marker WGA-coupled Alexa488 (company ...
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bioRxiv - Immunology 2020Quote: ... IgG1 or IgG2c biotin detection antibodies (Thermofisher, UK) were used with Extravidin-peroxidase (Sigma Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... Alexa Fluor 488 anti-Mouse IgG1 (ThermoFisher, A21121), Alexa Fluor 350 anti-Mouse IgG2b (ThermoFisher ...
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bioRxiv - Microbiology 2023Quote: ... and rat IgG1 (14-4301-82; Thermofisher Scientific), mouse IgG1 ascites fluid (MOPC 21 ...
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bioRxiv - Cancer Biology 2023Quote: ... Alexa Fluor 647 Goat Anti-mouse IgG1 (Invitrogen) (binds the CD271 primary antibody) ...
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bioRxiv - Bioengineering 2023Quote: ... Mouse IgG1 kappa Isotype Control (Invitrogen, clone P3.6.2.8.1), Mouse IgG2a kappa Isotype Control ...
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bioRxiv - Neuroscience 2022Quote: ... mouse IgG1 anti-Ki67 (1:25, Fisher Scientific), rat IgG2a anti-MBP (1:300 ...
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bioRxiv - Immunology 2023Quote: ... anti-mouse IgG1 antibody (Invitrogen; cat. no. A10661), or anti-mouse IgG2a antibody (Invitrogen ...