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Citations for Thermo Fisher :
251 - 300 of 10000+ citations for Recombinant Mouse Pcsk9 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Biotinylated proteins were identified with Alexa Fluor 647-conjugated streptavidin (Invitrogen). Primary antibodies were incubated for 1 hr (1:1000 dilution in PBS with 1% BSA and 0.3% Triton X-100 ...
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Entorhinal cortex vulnerability to human APP expression promotes hyperexcitability and tau pathologybioRxiv - Neuroscience 2023Quote: ... biotinylated proteins were captured by streptavidin magnetic beads (88817; Thermofisher Scientific) in 1.5 mL Eppendorf LoBind tubes using 83uL beads per 1mg of protein (here 200 µg of protein with 16.6 µL of beads ...
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bioRxiv - Cell Biology 2020Quote: IPs with FLAG-tagged protein were performed with Protein G magnetic beads (Invitrogen™ Dynabeads™ Protein G; #10004D) and α-FLAG antibody (Monoclonal ANTI-FLAG® M2 antibody produced in mouse ...
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bioRxiv - Microbiology 2021Quote: ... were used to pull-down FLAG-tagged proteins and any associated proteins rather than the Dynabead Protein G (Invitrogen) pre-bound with anti-FLAG M2 antibody (SigmaAldrich ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were quantified with Pierce™ BCA Protein Assay Kit (Life Technologies) and the purity of the purified proteins was checked by SDS-PAGE and western blot using anti-His antibody (1:1000 ...
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bioRxiv - Neuroscience 2019Quote: ... Briefly recombinant Protein G Agarose beads (ThermoFisher cat# 15920010) were washed three times with PBS ...
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bioRxiv - Microbiology 2021Quote: ... 25 µL packed Recombinant Protein A-Sepharose 4B (Invitrogen) was used to capture the immune complexes for 1 hour at RT ...
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bioRxiv - Neuroscience 2022Quote: The following recombinant proteins were used: CaMKIIα (#PR4586C, Invitrogen), GST-CaMKIIβ (#02-110 ...
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bioRxiv - Microbiology 2021Quote: ... followed by incubation with recombinant Protein G agarose (Invitrogen) for 2 h at 4 °C ...
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bioRxiv - Microbiology 2022Quote: Recombinant protein expression and purification FreeStyle 293F (ThermoFisher Scientific) cells were grown to a density of 1×106 cells/mL at 37°C with 8% CO2 with regular 135 rpm agitation ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Recombinant proteins were produced in Expi293F cells (Gibco, #A14527) following the manufacturer’s directions ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant protein A/G conjugated to HRP (Thermo Fisher) diluted 1:500 in TBST was added each well and the plates were incubated for 1 hour at 37°C then washed four times with TBST ...
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bioRxiv - Microbiology 2023Quote: ... recombinant proteins were purified using Ni-NTA agarose (ThermoFisher), and then buffer exchanged into PBS and concentrated using Amicon Ultracel centrifugal filters (EMD Millipore).
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bioRxiv - Immunology 2023Quote: ... recombinant SARS-CoV-2 S protein (RP-87680, Invitrogen) or recombinant SARS-CoV-2 spike RBD protein (40592-V08H ...
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bioRxiv - Bioengineering 2023Quote: ... and recombinant human interleukin-2 (IL-2) protein (Invitrogen) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... 50 μl of Recombinant Protein G – Sepharose beads (Invitrogen) per condition were washed 3 times in IP buffer ...
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bioRxiv - Immunology 2024Quote: Recombinant proteins were expressed in Expi293F cells (Life technologies). Full-length spike proteins were cloned into the pCAGGS mammalian expression vector as previously described79,80 ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were next incubated in biotinylated secondary antibody for 1 hour at room temperature followed by incubation in fluorophore tagged horseradish peroxidase (Alexa Fluor; Thermofisher). Sections were washed thrice in PBS-T and counterstained with RNAscope DAPI for 30 seconds ...
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bioRxiv - Immunology 2020Quote: ... Conjugate immunostaining was performed with biotinylated monoclonal mouse anti-tubulin (Invitrogen) and Brilliant Violet 421-conjugated streptavidin (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: ... A biotinylated mouse-anti pig antibody (clone P2C11, Thermo Fisher Scientific) diluted 1:500 was used for detection ...
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bioRxiv - Immunology 2024Quote: ... Secondary antibodies biotinylated rat anti-mouse-IFN-γ (catalogue #13704285, Invitrogen) and biotinylated rat anti-mouse-IL4 (catalogue #13731281 ...
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bioRxiv - Molecular Biology 2022Quote: Recombinant Rep1ΔAD-His and its variants were incubated with 50 μl D–Galactose agarose beads (Thermo Scientific) overnight at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... goat anti-mouse IgG Fab recombinant secondary antibody (Invitrogen), anti-NKp46 (9E2 ...
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bioRxiv - Developmental Biology 2020Quote: ... 50 ng/ml recombinant mouse EGF (Thermo Fisher Scientific), 100 ng/ml recombinant human IGF-1 (BioLegend) ...
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bioRxiv - Immunology 2023Quote: ... Recombinant mouse (rm)IFN-γ was purchased from ThermoFisher Scientific.
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bioRxiv - Bioengineering 2021Quote: ... tagged with Alexa Fluor™ 647 (Microscale Protein Labelling Kit, Thermo Fisher) (HEL-647) ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein was purified using His-Pur Ni-NTA Resin (Thermo Scientific), equilibrated using 2 bed volumes of PBS pH=7.4 ...
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bioRxiv - Molecular Biology 2020Quote: 6xHis-tagged REV-ERBβ LBD (4 nM) was incubated with anti-His antibody conjugated to terbium (1 nM) (ThermoFisher #PV5863) and FITC-labeled NCoR ID1 or NCoR ID2 peptide (400 nM ...
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bioRxiv - Cell Biology 2019Quote: ... 1-2 μg His-tagged calcineurin was first bound to Ni-NTA-agarose or magnetic Dynabeads (Thermo Fisher Sci. USA) in base buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... mCherry-(backbone pLV-CMV-bc) and FLAG-His-tagged(backbone Pcdna3) p16INK4A constructs were created using gateway technology (Life Technologies). For cell cycle profiling ...
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bioRxiv - Microbiology 2022Quote: ... and P323L/G671S N-terminally tagged with 6×His were purified using Bac-to-Bac™ Baculovirus Expression System based on the manufacturer’s instruction (Gibco). Upon 3 dpi of NSP12-enconding baculoviruses into Sf9 cells ...
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bioRxiv - Molecular Biology 2022Quote: ... zipper tag via a 5XGGS linker region was co-transfected with N-terminally His-tagged human Leptin in suspension HEK293 FreeStyle cells (ThermoFisher) in the presence of 20 μM kifunensine (Dextra ...
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Enterohepatic Transcription Factor CREB3L3 Protects Atherosclerosis via SREBP Competitive InhibitionbioRxiv - Physiology 2020Quote: ... V5-tagged full-length mouse Creb3l3 cDNA was inserted into pcDNA3.1 (Invitrogen); GFP-tagged full-length mouse Creb3l3 cDNA was inserted into pEGFP (GFP-CREB3L3 ...
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bioRxiv - Microbiology 2019Quote: ... V5 tagged constructs were probed with anti-mouse V5 (Invitrogen #R960-25). Blots were developed using the ECL Plus detection reagent (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... and fluorescence tagged goat anti-mouse IgG Alexafluor 594 (1:200, Invitrogen) secondary antibodies ...
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bioRxiv - Molecular Biology 2021Quote: ... Myc-tagged mouse RHBDL4 and GFP were subcloned into pENTR4 vectors (Invitrogen). Recombinant adenovirus plasmids were homologously recombined with pAd/CMV/V5-DEST vectors (Invitrogen) ...
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bioRxiv - Neuroscience 2022Quote: ... purified mouse myelin tagged with pHrodo Red succinimidyl ester (1:100, ThermoFisher) or isolated human synaptoneurosomes from AD brains (Tai et al. ...
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bioRxiv - Microbiology 2021Quote: ... 5x-His mouse anti-tag was used at 1:500 (Invitrogen). Anti-mouse HRP secondary antibody was used at 1:10,000 (Invitrogen) ...
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bioRxiv - Molecular Biology 2022Quote: GST-tagged bait proteins were purified using Pierce GST Protein Interaction Pull-Down kit (Thermo-Fisher Scientific). To examine recombinant Pin1 phosphorylation ...
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bioRxiv - Cell Biology 2020Quote: To compare the presence of the nucleolar rim of mNG-tagged proteins in live and fixed cells we fixed mNG-tagged cells in 4% formaldehyde (Thermo Scientific, 28908).
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bioRxiv - Cancer Biology 2021Quote: ... Biotinylated RNA baits were used in a ratio 1:12 to Hi-C libraries (25 ng biotinylated RNA baits per 300 ng of Hi-C library) and supplemented with 30 units SUPERase-In (Ambion). Biotinylated RNA baits for capture DNA-Seq were used in a ratio of 1:3.33 (300 ng RNA baits per 1,000 ng genomic DNA library ...
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bioRxiv - Biochemistry 2020Quote: ... Biotinylated proteins were enriched with Pierce™ streptavidin bead (Thermo Scientific™). The protein pellets were mixed to a pre-washed streptavidin beads (100 mM Hepes buffer) ...
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bioRxiv - Biochemistry 2021Quote: ... Biotinylated proteins were enriched by using Pierce Streptavidin Agarose beads (Thermo Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... Biotinylated proteins were affinity purified with magnetic streptavidin beads (Thermo Fisher Scientific) (40 μL slurry per sample ...
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bioRxiv - Cell Biology 2021Quote: ... Biotinylated proteins were enriched using Dynabeads™ M-280 Streptavidin (Invitrogen, 11205D) and eluted by heating the beads in 2X Laemmli buffer for immunoblotting.
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bioRxiv - Cell Biology 2020Quote: ... Biotinylated proteins were enriched on 100 μl streptavidin Ultralink beads (Thermo Scientific) for 1.5 h at room temperature with rotation ...
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bioRxiv - Cell Biology 2021Quote: ... Biotinylated proteins were purified on High Capacity Streptavidin Agarose beads (Thermo Scientific) in RIPA buffer and proteins were identified by mass spectrometry.
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bioRxiv - Microbiology 2020Quote: ... Biotinylated proteins were detected with Alexa-fluor-488-streptavidin (Molecular Probes S32354) and captured with Streptavidin High-Capacity Agarose beads (Thermo Scientific PI20359 ...
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bioRxiv - Plant Biology 2019Quote: ... Biotinylated proteins were detected with streptavidin-HRP (21124, Thermo Scientific, Rockford, IL).
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bioRxiv - Plant Biology 2019Quote: ... Biotinylated protein was detected with streptavidin-HRP (21124, Thermo Scientific, Rockford, IL).