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Citations for Thermo Fisher :
501 - 550 of 10000+ citations for Recombinant Mouse Pcsk9 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... or membrane probed with mouse monoclonal 6x-His tag antibody (4A12E4) (Thermofisher, Waltham, MA) (Supplementary Figure 2).
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bioRxiv - Bioengineering 2023Quote: ... were first coated with 100 ng of mouse anti-His antibody (Invitrogen, MA1-21315) and blocked ...
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bioRxiv - Cell Biology 2019Quote: ... Biotinylated proteins were isolated by incubating the cell lysates with Avidin-coated beads (ThermoFisher Scientifc) for 1h at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Biotinylated proteins were captured by incubation with high capacity neutravidin-conjugated beads (Thermo Fisher Scientific) for 3 hours at 4 degrees Celsius ...
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bioRxiv - Physiology 2020Quote: ... Biotinylated cell surface proteins were affinity-purified using NeutrAvidin-conjugated agarose beads (Thermo Fisher Scientific), and purified cell surface proteins were analyzed by western blot using the mαNaVPAN antibody (1:2000 ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were prepared and the biotinylated proteins were precipitated with streptavidin agarose resin (ThermoFisher Scientific) and detected by western blot.
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bioRxiv - Microbiology 2021Quote: ... University of Utah) and biotinylated proteins were detected with streptavidin Alexa-Fluor-488 (Invitrogen S32354). Primary antibodies were detected with goat polyclonal Alexa Fluor-conjugated secondary antibodies (Invitrogen) ...
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bioRxiv - Bioengineering 2021Quote: ... Phages bound to biotinylated target protein were captured by streptavidin coupled Dynabeads (Thermo Fisher Scientific), washed and eluted in 100 nM triethylamine ...
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bioRxiv - Molecular Biology 2019Quote: ... biotinylated proteins were isolated by incubation with 200μl of Dynabeads (MyOne Streptavidin C1; Life Technologies) overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: Cell surface proteins were biotinylated using an EZ-Link Sulfo-NHS-Biotin reagent (Thermo Scientific) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... Biotinylated proteins were enriched from the supernatant on High-Capacity Neutravidin Agarose resin (ThermoFisher Scientific) (0.5 mL of 50% resin slurry) ...
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bioRxiv - Neuroscience 2022Quote: ... (iv) Biotinylated proteins are affinity-purified with streptavidin agarose resin (Thermo Scientific, Cat no. 20349). The use of neutravidin agarose resin yielded the same results ...
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bioRxiv - Cancer Biology 2023Quote: ... and biotinylated proteins were extracted from the lysates using Pierce Streptavidin Magnetic Beads (ThermoFisher 88816) per the manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: ... the biotinylated LRRK2RCKW protein was immobilized to MyOne T1 streptavidin-coated beads (Thermo Fisher Scientific). The ribosome display selections were performed using a KingFisher Flex MTP96 well platform as described previously (Dreier and Plückthun ...
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bioRxiv - Immunology 2023Quote: Protein antigens were biotinylated using EZ-link Sulfo-NHS-Biotin No-Weigh kit (Thermo Fisher) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... was biotinylated (Invitrogen) to a ratio of 1 biotin molecule per OVA as previously described (Brooks et al. ...
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bioRxiv - Neuroscience 2021Quote: The secondary antibodies used were: biotinylated anti-mouse IgG (Invitrogen cat#62-6540, 1:1k), Alexa Fluor 488 donkey anti-rabbit IgG (Invitrogen cat#A21206 ...
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bioRxiv - Cell Biology 2023Quote: ... 5FU was detected using an anti-mouse biotinylated secondary antibody (Thermo Fisher Scientific, Cat. # A10519) at 1:200 dilution in 1% BSA/PBS for 1 h at RT followed by incubation with streptavidin-conjugated 488 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Total protein concentration of the lysates was measured via BCA assay and biotinylated proteins were immunoprecipitated using Streptavidin magnetic beads (Thermo Fisher). 500μg magnetic beads were incubated with up to 70μg of lysate rotating overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... The same amount of protein was used for the enrichment of biotinylated proteins using100 μl Pierce™ streptavidin agarose beads (Cat. # 20353, ThermoFisher) overnight at 4 °C ...
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bioRxiv - Physiology 2023Quote: Cell surface proteins from transfected HEK293 cells were biotinylated using the Pierce Cell Surface Protein Isolation Kit (ThermoFisher Scientific., cat# 89881). Briefly ...
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bioRxiv - Immunology 2023Quote: ... Expression constructs for M35-V5/His and M27-V5/His (both in pcDNA3.1-V5/His, Invitrogen) have been described previously (Munks et al ...
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bioRxiv - Biochemistry 2022Quote: Recombinant proteins were labeled with Alexa Fluor(tm) 647 NHS ester (Thermo Fisher) as described previously18 ...
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bioRxiv - Biochemistry 2022Quote: ... the recombinant protein mixtures were combined with 12.5 μM diFMUP (Life Technologies, E12020), added to 384-well plates ...
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bioRxiv - Immunology 2019Quote: Chimeric VLPs or monomeric recombinant proteins were coated onto Maxisorp microtiter plates (Nunc) at 1μg/ml in PBS and incubated overnight at 4°C ...
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LIR-dependent LMX1A/LMX1B autophagy crosstalk shapes human midbrain dopaminergic neuronal resiliencebioRxiv - Cell Biology 2019Quote: ... the recombinant proteins were dialyzed using Slide-A-Lyzer™ cassettes (Thermo Fisher) into cold coupling buffer containing 100mM NaHCO3 and 500mM NaCl ...
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bioRxiv - Bioengineering 2021Quote: ... Recombinant protein expression was triggered using 0.5mM isopropyl β-D-1-thiogalactopyranoside (ThermoFisher) and incubated for 18 h in an orbital shaker at 18 °C ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant Spike protein was labelled with Alexa Fluor™ 546 (Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... The recombinant N protein was purified using Ni2+ resin (Thermo Fisher Scientific, USA). An indirect ELISA assay was conducted with the recombinant N protein as the coating antigen (3 ng/μl ...
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bioRxiv - Cancer Biology 2022Quote: ... and purified recombinant Cas9 proteins (250 ng/ml: Thermo Fisher Scientific, MA, USA). The gRNA sequences are as follows:
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bioRxiv - Biochemistry 2019Quote: ... LRRK2 (G2019S) Recombinant Human Protein (residues 970-2527 #10499963) was purchased from Invitrogen and all additional kinases used in the in vitro screen were from MRC PPU Reagents and Services – including TAK1 (1-303) ...
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HIV-1 Nef interacts with LMP7 to attenuate immunoproteasome formation and MHC-I antigen presentationbioRxiv - Microbiology 2019Quote: ... The samples were mixed with recombinant protein G-Sepharose 4B beads (Thermo Fisher) and 1 µl anti-Flag antibodies (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: This recombinant S protein was overexpressed using the HEK 293F mammalian cells (Invitrogen) at 37°C under 5% CO2 in a Multitron-Pro shaker (Infors ...
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bioRxiv - Biochemistry 2021Quote: Recombinant proteins were labeled with Alexa Fluor™ 647 NHS ester (Thermo Fisher) as described previously.20 In brief ...
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bioRxiv - Biophysics 2021Quote: ... 50 nM Pierce™ recombinant protein A (#21184, Thermo Fisher Scientific, Waltham, USA) 50 nM bovine serum albumin (#A4612 ...
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bioRxiv - Biochemistry 2022Quote: All recombinant proteins were produced in Rosetta2 Escherichia coli competent cells (Thermo Fisher) induced with 0.4 mM isopropyl-β-d-1-thiogalactoside (IPTG ...
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bioRxiv - Microbiology 2023Quote: ... Secondary horseradish peroxidase (HRP)-conjugated recombinant A/G protein (Invitrogen, lot number XH346151) diluted 1:20,000 was used for detection and visualized with KPL TMB two-component peroxidase substrate kit (SeraCare ...
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bioRxiv - Physiology 2023Quote: ... Recombinant proteins were produced in suspension culture in Freestyle 293F cells (Life Technologies) transiently transfected using FectroPro reagent (Polyplus) ...
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bioRxiv - Cell Biology 2023Quote: ... all iPSCs were cultured in Vitronectin Recombinant Human Protein (VTN-N) (ThermoFisher, A14700) pre-coated 6-well plates with 2 mL TeSR-E8 medium (Life Technologies ...
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bioRxiv - Immunology 2024Quote: Recombinant proteins were coated on Immulon 4 HBX 96-well plates (Thermo Scientific) at a concentration of 2 μg/mL in PBS ...
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bioRxiv - Genetics 2019Quote: ... Proteins tagged with the V5 epitope (Hxk2, Reg1) were detected with the Anti-V5 probe (Invitrogen) diluted 1:1,000 ...
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bioRxiv - Microbiology 2023Quote: ... we used PrepEase Histidine-tagged Protein Purification Midi Kit-High Yield (Affymetrix, Santa Clara, CA, USA) or Protino Ni-IDA 2000 packed columns (Macherey-Nagel ...
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bioRxiv - Genetics 2023Quote: ... Flag-tagged proteins were immunoprecipitated with anti-Flag magnetic agarose (Pierce Anti-DYKDDDDK Magnetic Agarose, ThermoFisher), according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: rox2RNA probes at 100 nM and hrp38 RNA probes at 50nM were incubated with MBP-tagged FL CLAMPWT protein or MBP-tagged FL CLAMPdelPrLD protein in REMSA binding buffer provided with the LightShift Chemiluminescent RNA EMSA kit (Thermo Scientific, USA) at room temperature for 30 min according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... The His-mCherry-fusion proteins were purified using a Ni-NTA column (Thermo Fisher Scientific). The eluted proteins were dialyzed in 1 L dialyzed buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2019Quote: ... cells were activated by adding 50 ng/mL recombinant mouse IFN gamma (Invitrogen). On day 7 ...
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bioRxiv - Microbiology 2022Quote: ... and stimulated with 10 ng/mL (~100 U/mL) recombinant mouse IFNγ (Gibco) for 24 h prior to infection ...
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bioRxiv - Immunology 2024Quote: ... the cells were probed with donkey anti-mouse secondary antibody tagged with Alexa Fluor 568 (Invitrogen), for 1 h in dark ...
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bioRxiv - Microbiology 2023Quote: ADCP was measured using a flow cytometry-based phagocytosis assay.98 Biotinylated recombinant HIV-1 gp120 from strain CN54 (Acro Biosystems; catalog #GP0-V182E6) was combined with fluorescent NeutrAvidin beads (Life Technologies; catalog #F8776) at a concentration of 1 µg protein per µl bead and incubated overnight at 4°C ...
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bioRxiv - Bioengineering 2023Quote: Rhodamine-tagged dextran (Invitrogen) was encapsulated into AlgLung and S-AlgLung hydrogels at a concentration of 0.5 mg/ml ...