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Citations for Thermo Fisher :
401 - 450 of 10000+ citations for Recombinant Mouse Map2k1 His & GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... The membrane was incubated with GST-TAG antibody (1:1000, ThermoFisher) or anti-MC4 (rabbit ...
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bioRxiv - Microbiology 2019Quote: ... GST-tag and 3xFLAG-tag fusions were achieved using pDEST27 (Invitrogen) and pCI-neo-3xFLAG vector ...
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bioRxiv - Molecular Biology 2020Quote: ... The GST tag was removed using HRV 3C protease (Thermo Scientific) with an enzyme to substrate ratio of 1:100 at 4°C for 16 hrs ...
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bioRxiv - Biochemistry 2023Quote: ... and polyclonal anti-GST (Thermo Fisher Scientific, Waltham, MA, USA, CAB4169) antibodies ...
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bioRxiv - Molecular Biology 2020Quote: ... Alexa555 or Alexa 594 tagged secondary antibodies (Invitrogen) were used ...
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bioRxiv - Cell Biology 2019Quote: ... fluorescent Alexa-tagged antibodies were from Molecular Probes.
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bioRxiv - Neuroscience 2020Quote: ... fluorescently-tagged polystyrene microspheres (10 micron, Invitrogen F8836) were bilaterally injected into the anterior chambers of Opn4Cre;Ai32 mice at ~6-8 weeks of age (Sappington et al. ...
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bioRxiv - Microbiology 2021Quote: ... Fluorescence-tagged secondary antibodies were purchased from Thermofisher and used at 1:500 dilutions ...
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bioRxiv - Cell Biology 2021Quote: ... horseradish peroxidase-tagged second antibody (Thermo Fisher Scientific) was applied for 1 h at room temperature and the signal visualized using SuperSignal enhanced chemiluminescent (ECL ...
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bioRxiv - Microbiology 2021Quote: ... and Alexa488-tagged phalloidin were obtained from Invitrogen and were used at 1:1000 dilution.
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bioRxiv - Molecular Biology 2020Quote: ... fluorescent Alexa-tagged antibodies were from Molecular Probes. VOA ...
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bioRxiv - Cancer Biology 2022Quote: ... Alexa555 or Alexa 594-tagged secondary antibodies (Invitrogen) were used ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... CXCL1 and CXCL2 (FAM tagged; Thermo Fisher Scientific) on a Biorad CFX Connect™ machine ...
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bioRxiv - Developmental Biology 2022Quote: ... An appropriate Alexa Fluor-tagged secondary antibody (Invitrogen) was matched to the primary and incubated for 1-2 h at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... 1:1000 mouse anti-his capture antibody in PBS was coated onto Immulon 2 HB 96-well plates (Thermo Fisher Scientific) at 4°C overnight ...
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bioRxiv - Genetics 2023Quote: ... The blots containing yeast Nap1 were treated similarly but incubated with mouse anti-HIS primary antibody (Thermo Fisher Scientific, Waltham, MA) and HRP-conjugated sheep anti-mouse secondary antibody (Kindle biosciences ...
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bioRxiv - Immunology 2019Quote: ... in OT-1 culture medium supplemented with 100 U/ml mouse recombinant IL-2 (Thermo Fisher Scientific, catalog 14802164) in a 37 °C/5% CO2 incubator ...
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bioRxiv - Cancer Biology 2019Quote: ... The following additional niche factors were added to generate “Full” media: 50 ng/ml mouse recombinant EGF (Life Technologies), 100 ng/ml mouse recombinant Noggin (Peprotech) ...
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bioRxiv - Physiology 2023Quote: A recombinant adenovirus expressing mouse Sult2b1 (Ad-Sult2b1) was produced using the ViraPower Adenoviral Expression System (Invitrogen, Carlsbad, CA), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Endpoint PCR for all mouse CASK experiments was performed using 0.25 U recombinant Taq DNA Polymerase (Thermo Fisher Scientific) with 2 mM MgCl2 ...
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bioRxiv - Neuroscience 2023Quote: Four sections from each mouse were stained using primary antibodies calbindin D28K anti-rabbit recombinant polyclonal (1:200; ThermoFisher) and parvalbumin anti-mouse monoclonal (1:200 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transfected with the recombinant pcDNA3.1 plasmid (6 μg/flask) containing mouse Wnt10b cDNA using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and further ligated into pcDNA 3.1 V5/His TOPO vector using pcDNA 3.1 V5/His TOPO TA cloning kit (Invitrogen). The ACRD etoll was then transferred to pGADT7-AD vector using Nde1 and BamH1 sites ...
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bioRxiv - Cell Biology 2022Quote: ... The His SNX32ΔC protein bound to the lipids was detected by anti-His antibody [1:10,000 (Thermo Scientific)]
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bioRxiv - Molecular Biology 2023Quote: ... ceSMSγ and ceSMSr cDNAs were cloned into yeast expression vector pYES2.1/V5-His-TOPO and mammalian expression vector pcDNA3.1/V5-His-TOPO (Invitrogen) as described previously(18).For expression studies in Drosophila S2 cells ...
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bioRxiv - Developmental Biology 2024Quote: HEK293T cells were transferred with C-terminal HA-tagged Kayak and N-terminal GFP-tagged Jun-epi or Kayak using Lipofectamine 2000 (11668030, Thermo Fisher). The GFP-trap agarose beads (ABIN509397 ...
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bioRxiv - Genetics 2021Quote: ... 12 µL Hi-Di Formamide (Applied Biosystems) and 0.3 µL MapMarker 500 labelled with DY-632 (Eurogentec) ...
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bioRxiv - Microbiology 2019Quote: ... we used Dynabeads™ His-Tag (Invitrogen) for Ni-affinity purification ...
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bioRxiv - Bioengineering 2022Quote: 6x-His tag antibodies (Invitrogen, MA1-21315) were coated at 2 µg/mL in PBS onto 96-well half- area high binding plates (Corning ...
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bioRxiv - Immunology 2021Quote: ... blot using an anti-His Ab (ThermoFisher). MSP-142 and AMA-1 were biotinylated and tetramerized with streptavidin-PE (SA-PE ...
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bioRxiv - Microbiology 2021Quote: ... eluted in Hi-Di formamide (Applied Biosystems) with ROX-350 ladder ...
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bioRxiv - Biochemistry 2021Quote: ... anti-His (MA1-21315, Invitrogen, 1:1,000). Proteins separated by SDS-PAGE were detected following the manufacturer’s guidelines (GE Healthcare ECL Western Blotting detection system ...
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bioRxiv - Microbiology 2020Quote: Hi-sensitivity Qubit DNA kit (Thermo Fisher) was used to measure the DNA concentration directly from the lysate ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% HI FBS (Gibco 10500), 20% D4T conditioned medium (24) ...
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bioRxiv - Microbiology 2020Quote: ... and cloned into pcDNA-V5/His (Invitrogen). The M fragment of CCHFV was PCR amplified with primers shown in Table II from cDNA generated from CCHFV genomic RNA ...
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bioRxiv - Biochemistry 2020Quote: ... was loaded onto His Dynabeads (Invitrogen 10103D), relying on the intrinsic metal-binding properties of untagged PrP ...
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bioRxiv - Zoology 2019Quote: ... Primary (rabbit anti-His, Thermo Fisher Scientific) and secondary (anti-rabbit Alexa Fluor 594 ...
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bioRxiv - Synthetic Biology 2020Quote: ... supplemented with 10% HI FBS (Thermo Fisher). 30,000 HEK293AAV cells were added to each well of a 48 well plate one day before transfection ...
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bioRxiv - Cell Biology 2020Quote: ... 6x-His Tag antibody is from ThermoFisher Scientific (MA1-21315-D550) ...
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bioRxiv - Immunology 2020Quote: ... with 10 % V/V Hi FCS (Gibco), 1 mM sodium pyruvate (Gibco ...
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bioRxiv - Biochemistry 2021Quote: ... terbium-coupled anti-his at 1nM (Invitrogen) and decreasing concentrations of compounds in 384-well microplates (Corning ...
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bioRxiv - Neuroscience 2021Quote: ... HI Horse Serum (Invitrogen, 2% vol/vol), GlutaMax (2 mM ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 17.4 μl Hi-Di Formamide (Applied Biosystems), and 2 μL of RT reaction mixture were loaded in a 96-well plate ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Anti-His antibody was from Thermo Fisher Scientific (37-2900 ...
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bioRxiv - Cell Biology 2022Quote: ... anti-His tag (ThermoFisher Scientific, MA1-21315), and anti-tubulin (DHSB ...
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bioRxiv - Zoology 2022Quote: ... AeStaufen cDNA synthesized by Genscript was cloned into pIZT/ V5-His (Invitrogen). The same plasmid containing Chloramphenicol acetyltransferase (CAT ...
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bioRxiv - Molecular Biology 2023Quote: ... The antibody against His (631212, Fisher Scientific) is commercially available ...
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bioRxiv - Neuroscience 2023Quote: The plasmid pcDNA6.1/V5-His B (Thermofisher) was used as the template to subclone in the full-length PNPLA6 cDNA (from RefSeq transcript NM_001166111.1) ...
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bioRxiv - Microbiology 2023Quote: ... and probed with □-His mAb (Thermo Scientific) diluted 1:1,000 in 6% milk in PBS-T followed by α-mouse IgG HRP (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... His Pur cobalt spin columns (Thermo Scientific) were used to purify the in vitro-translated proteins ...