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Citations for Thermo Fisher :
201 - 250 of 10000+ citations for Recombinant Mouse Map2k1 His & GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Mouse IFN-alpha 2 (Ifna2) recombinant protein was purchased from Affymetrix eBioscience (catalog #14-8312) ...
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bioRxiv - Genetics 2021Quote: ... 50 ng/ml recombinant mouse epidermal growth factor (EGF; Gibco, PMG8041), 100 ng/ml recombinant murine Noggin (PeproTech ...
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bioRxiv - Cell Biology 2022Quote: ... 10 ng/ml mouse recombinant LIF (ES cell-tested) (Gibco #A35935). Upon reaching confluence ...
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bioRxiv - Genetics 2023Quote: ... 50 ng/ml recombinant mouse epidermal growth factor (EGF; Gibco, PMG8041), 100 ng/ml recombinant murine Noggin (PeproTech ...
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bioRxiv - Cancer Biology 2022Quote: ... 1% penicillin/streptomycin and 50ng/ml recombinant mouse EGF (Life Technologies) was used for culturing ApcKO colon organoids ...
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bioRxiv - Cancer Biology 2024Quote: ... 1% penicillin/streptomycin and 50ng/ml recombinant mouse EGF (Life Technologies) was used for culturing colon organoids ...
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bioRxiv - Biochemistry 2021Quote: ... or pT7CFE1-NHIS-GST (ThermoFisher) vectors for expression as N-terminal GST fusion proteins (Collaboration 2016) ...
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bioRxiv - Neuroscience 2020Quote: ... GST-AAK1 (1-501aa) (Thermofisher) with or without GST-AP2M1-WT or T156A protein were subjected to a kinase reaction in kinase buffer (20 mM HEPES pH=7.4 ...
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bioRxiv - Microbiology 2023Quote: ... anti-GST (Invitrogen A-5800), anti-HA (Sigma H6908) ...
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bioRxiv - Cell Biology 2023Quote: ... before anti-GST antibody (Invitrogen) binding and detection as described above.
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bioRxiv - Immunology 2020Quote: ... HEK293T cells were transfected with N-terminally 3XFLAG and V5-tagged wild type mouse ZBP1 or Zα1α2-mutant mouse ZBP1 transducing vectors in the pLenti6 backbone (Life Technologies) (Maelfait et al. ...
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bioRxiv - Microbiology 2020Quote: One 96-well plate was coated with 200ng/well of recombinant N protein and another plate was coated with 200ng/well of recombinant 6xHis-tagged SARS-CoV-2 spike protein receptor binding domain (RBD) produced using the FreeStyle 293 Expression System (Thermo Fisher) from BEI Resources (Cat# NR-52366 ...
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bioRxiv - Cell Biology 2021Quote: ... The His-tagged recombinant fusion protein was separated by cobalt immobilized metal chelate affinity beads using the HisPur Cobalt Purification Kit (Thermo Scientific) following the procedure described by the manufacturer ...
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bioRxiv - Cell Biology 2020Quote: Recombinant 6XHis-tagged TbVps24 (N-terminus tag) was cloned using the pTrcHis TOPO TA expression system (Thermo Fisher Scientific, Carlsbad, CA) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... and counter-stained with fluorophore-tagged goat anti-mouse IgG (H+L) antibody (Invitrogen), before being acquired with BD FACSCanto II (BD Biosciences) ...
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bioRxiv - Microbiology 2020Quote: ... SV5-tagged proteins were detected using mouse anti-SV5 mAb (1:5000, Life Technologies); HA-tagged proteins were detected by anti-HA mAb (clone HA-7 ...
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bioRxiv - Neuroscience 2023Quote: ... HA-tagged scFvs were detected using mouse mAb 2-2.2.14 (ThermoFisher Catalog# P126183, RRID:AB_10978021). The following day sections were washed 4 x 5 min with vehicle ...
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bioRxiv - Bioengineering 2020Quote: ... T cells were cultured in RPMI-1640 supplemented with 10% HI-FBS and fed with recombinant human IL-2 (ThermoFisher) and IL-15 (Miltenyi ...
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bioRxiv - Microbiology 2022Quote: ... A recombinant SPATR (rSPATR) fused to a polyhistidine tag (His-tag) was produced in BL21-CodonPlus-RIL competent cells (Invitrogen) and protein purification was performed under denaturing conditions using Ni-NTA Agarose (Qiagen ...
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bioRxiv - Systems Biology 2023Quote: ... T cells were expanded in RPMI-1640 media supplemented with 10% heat-inactivated FBS (HI-FBS) as well as recombinant human IL-2 (50 U/mL; ThermoFisher) and IL-15 (1 ng/mL ...
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bioRxiv - Biochemistry 2023Quote: Plasmids for recombinant protein expression were constructed using mega primer insertion PCR [64] using the plasmid Champion pET303/CT-His vector (Invitrogen). The double-stranded DNA inserts (Integrated DNA Technologies ...
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bioRxiv - Immunology 2022Quote: ... The plate was coated with 2 µg/mL mouse anti-His antibody (Invitrogen cat ...
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bioRxiv - Bioengineering 2021Quote: ... Blocking buffer was refreshed and mouse anti-His antibody (Thermofisher, Cat#MA1-21315) was added at a dilution factor of 1:2000 in blocking buffer ...
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bioRxiv - Microbiology 2024Quote: ... at a 1:5,000 dilution and mouse anti-His monoclonal antibody (Thermo Fisher Scientific or Abcam ab216773 ...
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bioRxiv - Neuroscience 2020Quote: ... sections were rinsed in 0.1 M PBS and incubated in Alexa Fluor 647-tagged goat anti-mouse IgG or Alexa Fluor 750-tagged goat anti-rabbit IgG (1:100, Thermo Fisher, cat# A21235 and A21039), or Alexa Fluor 647-tagged donkey anti-mouse IgG ...
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bioRxiv - Microbiology 2021Quote: The production of His-tagged soluble ACE2 was performed in FreeStyle™ 293-F cells and purified using HisPur™ Ni-NTA Resin (Thermo Scientific) as described previously [31].
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bioRxiv - Synthetic Biology 2020Quote: ... and the His-tagged proteins on the cell surface were then visualized with SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, USA) using FUSION Solo S documentation system (Vilber ...
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bioRxiv - Cell Biology 2021Quote: ... The second step was the separation of mCherry-zyxin or EGFP-VASP from 6xHis-MBP and the removal of the His-tagged TEV protease by Ni-NTA chromatography (HisPur™ Ni-NTA Resin, Thermo Scientific: 88222). First ...
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bioRxiv - Biophysics 2022Quote: ... anti-His tag antibody molecules were tagged with the dye Alexa Fluor® 647 NHS Ester (Succinimidyl Ester, ThermoFisher SCIENTIFIC, Catalog number: A20006) using the same procedure as followed in our previous paper.(11 ...
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bioRxiv - Neuroscience 2023Quote: ... SH-SY5Y cells were transfected with either an empty vector or with a vector expressing His-tagged human p35 (gift from Dr. Harish Pant) using Lipofectamine 3000 (Life Technologies, Carlsbad, CA). Two days after the transfection ...
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bioRxiv - Cell Biology 2020Quote: ... Clarified lysates were then incubated with 20 μg of recombinant glutathione S-transferase (GST)-RBD-MICAL-L1 and Pierce™ Glutathione magnetic agarose beads (Life Technologies-Invitrogen, cat# 78601) for 2 hrs at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... 20 ng/ml recombinant mouse macrophage colony-stimulating factor (Gibco, Burlington, ON), and penicillin/streptomycin antibiotics ...
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bioRxiv - Cancer Biology 2022Quote: ... 50 µg/ml mouse recombinant epidermal growth factor (EGF; Thermo Fisher Scientific), 10nM [Leu15]-gastrin I (Merck) ...
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bioRxiv - Pathology 2021Quote: ... His-tag recombinant SARS-CoV-2 Spike Protein S1/S2 (S-ECD) (1 ng/ml; ThermoFisher Scientific; aa11-1208; RP-87680) or Flag-tag recombinant SARS-CoV-2 Spike RBD (1 ng/ml ...
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bioRxiv - Biochemistry 2023Quote: ... the elutes of GlnR147 (his-TEV GlnR C60SL147C) from Ni-NTA column were cleaved with recombinant tobacco-etch virus protease (Life Technologies) overnight at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... Lanes reacted with anti-His antibody were incubated for 1.5 hours in mouse anti 6X His antibody (Invitrogen MA1-21315, 1:500 in TBST) and lanes reacted with S-tag antibody were incubated in rabbit anti-mouse S-tag antibody (Invitrogen PIPA 581631 ...
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Cdc42 promotes epithelial morphogenesis by coupling Par-complex and Crumbs recruitment via Par6-aPKCbioRxiv - Cell Biology 2019Quote: ... was used to generate plasmids GST::Cdc42N17 and GST::Cdc42N17using the Gateway™ Cloning System (Invitrogen). The GST::Crbıntra and GST::CrbıntraΔERUconstructs were provided by E ...
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bioRxiv - Plant Biology 2021Quote: ... 4 ng GST or GST-VDAL purified protein were loaded onto 10 kDa microspin columns (Ambion). After washed twice with 1 mL HS buffer (50 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: Mouse hemagglutinin (HA) tagged Lphn2 construct was synthesized by GeneArt Gene Synthesis (Thermo Fisher Scientific) and cloned into the Gateway system pENTR221 vector (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... Clarified lysates were then incubated with 20 μg of recombinant glutathione S-transferase (GST)-RBD-MICAL-L1 and Pierce™ Glutathione magnetic agarose beads (Life Technologies-Invitrogen, cat# 78601) for 2 hrs at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... GST-tag (ThermoFisher Scientific #8-326), HIS-tag (Sigma-Aldrich H1029) ...
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bioRxiv - Neuroscience 2019Quote: GST-LRRK2 (aa 970-2527, Invitrogen) was incubated with His-HDAC6 (EMD Millipore ...
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bioRxiv - Genetics 2021Quote: ... and Anti-GST (Invitrogen, A-5800). Anti-Flag immunoprecipitations were carried out using magnetic beads pre-conjugated with the antibody (Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... GST-14-3-3s constructs (Invitrogen) were transformed into Escherichia coli strain BL21 (DE3 ...
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bioRxiv - Microbiology 2022Quote: ... or membrane probed with mouse monoclonal 6x-His tag antibody (4A12E4) (Thermofisher, Waltham, MA) (Supplementary Figure 2).
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bioRxiv - Bioengineering 2023Quote: ... were first coated with 100 ng of mouse anti-His antibody (Invitrogen, MA1-21315) and blocked ...
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bioRxiv - Cell Biology 2021Quote: ... flow-through and washes were processed for western blotting using mouse anti-GST (at 1/1000 dilution; Invitrogen), followed by goat anti-Mouse IgG-HRP antibodies as described in the western blotting section.
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bioRxiv - Immunology 2023Quote: ... Expression constructs for M35-V5/His and M27-V5/His (both in pcDNA3.1-V5/His, Invitrogen) have been described previously (Munks et al ...
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bioRxiv - Plant Biology 2020Quote: ... The GST and GST-XIK fusion proteins were extracted with Pierce Immobilized Glutathione resin (Thermo Fisher Scientific) with the protein interaction buffer (20 mM Hepes-KOH pH7.2 ...
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bioRxiv - Cell Biology 2019Quote: ... GST or GST-EB1 (5 μg) were incubated with 20 μl glutathione agarose beads (16100; Thermo Scientific) for 2 h at 4°C ...