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Citations for Thermo Fisher :
401 - 450 of 10000+ citations for Recombinant Human PDCD1 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... 50 μl of Recombinant Protein G – Sepharose beads (Invitrogen) per condition were washed 3 times in IP buffer ...
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bioRxiv - Immunology 2024Quote: Recombinant proteins were expressed in Expi293F cells (Life technologies). Full-length spike proteins were cloned into the pCAGGS mammalian expression vector as previously described79,80 ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were next incubated in biotinylated secondary antibody for 1 hour at room temperature followed by incubation in fluorophore tagged horseradish peroxidase (Alexa Fluor; Thermofisher). Sections were washed thrice in PBS-T and counterstained with RNAscope DAPI for 30 seconds ...
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bioRxiv - Microbiology 2020Quote: ... and cultured in RPMI medium supplemented with 10% FBS and differentiated for 7 days with 20 ng/mL recombinant human Macrophage-Colony Stimulating Factor protein (M-CSF) (Thermo Fisher Scientific), and 100U/L of penicillin-streptomycin solution (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: Recombinant Rep1ΔAD-His and its variants were incubated with 50 μl D–Galactose agarose beads (Thermo Scientific) overnight at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... 1.0 mg/mL recombinant human insulin, 0.55 mg/mL human transferrin, 0.67 μg/mL sodium selenite, Gibco), and 0.04 μg/mL dexamethasone (Sigma Aldrich) ...
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bioRxiv - Bioengineering 2021Quote: ... tagged with Alexa Fluor™ 647 (Microscale Protein Labelling Kit, Thermo Fisher) (HEL-647) ...
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bioRxiv - Molecular Biology 2020Quote: ... V5-tagged proteins were detected with mouse monoclonal anti-V5 antibody (ThermoFisher) at 0.3 μg/ml ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein was purified using His-Pur Ni-NTA Resin (Thermo Scientific), equilibrated using 2 bed volumes of PBS pH=7.4 ...
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bioRxiv - Molecular Biology 2020Quote: 6xHis-tagged REV-ERBβ LBD (4 nM) was incubated with anti-His antibody conjugated to terbium (1 nM) (ThermoFisher #PV5863) and FITC-labeled NCoR ID1 or NCoR ID2 peptide (400 nM ...
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bioRxiv - Cell Biology 2019Quote: ... 1-2 μg His-tagged calcineurin was first bound to Ni-NTA-agarose or magnetic Dynabeads (Thermo Fisher Sci. USA) in base buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... mCherry-(backbone pLV-CMV-bc) and FLAG-His-tagged(backbone Pcdna3) p16INK4A constructs were created using gateway technology (Life Technologies). For cell cycle profiling ...
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bioRxiv - Microbiology 2022Quote: ... and P323L/G671S N-terminally tagged with 6×His were purified using Bac-to-Bac™ Baculovirus Expression System based on the manufacturer’s instruction (Gibco). Upon 3 dpi of NSP12-enconding baculoviruses into Sf9 cells ...
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bioRxiv - Immunology 2019Quote: ... and the ELISPOT plate was incubated with biotinylated goat anti-human IgG Fc (Invitrogen), followed by HRP-conjugated avidin (Vector Laboratories ...
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bioRxiv - Bioengineering 2021Quote: ... nanovials were modified with biotinylated goat anti-human IgG Fc antibody (Thermo Fisher Scientific). Samples were resuspended in DMEM and seeded in a well plate ...
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bioRxiv - Immunology 2019Quote: ... The sCD4 binding was detected using a biotinylated Human anti-CD4 (Thermo Fisher Scientific) followed by HRP-conjugated Streptavidin ...
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bioRxiv - Cell Biology 2024Quote: ... biotinylated human serum albumin b-HSA (70 kg/mol, ACROBiosystems HSA-H82E3, Fisher Scientific), biotinylated heparan sulfate b-HS (synthesized using hydrazone ligation to 12 kg/mol HS and characterized by QCM-D to determine % of biotinylation as described in Thakar et al ...
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bioRxiv - Molecular Biology 2022Quote: GST-tagged bait proteins were purified using Pierce GST Protein Interaction Pull-Down kit (Thermo-Fisher Scientific). To examine recombinant Pin1 phosphorylation ...
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bioRxiv - Neuroscience 2019Quote: ... and 20 ng/mL recombinant human fibroblast growth factor-basic (Life Technologies). After 3 to 6 weeks ...
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bioRxiv - Microbiology 2021Quote: ... 20 ng/ml of recombinant human epidermal growth factor (EGF, Life Technologies), human insulin (Sigma) ...
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bioRxiv - Neuroscience 2022Quote: ... 10 ng/ml recombinant human basic fibroblast growth factor (Invitrogen, Waltham, MA) (hiPSC medium).
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bioRxiv - Physiology 2020Quote: ... and 2.5 ng/ml recombinant human hepatocyte growth factor (Gibco, Loughborough, UK). Skeletal muscle myoblasts were incubated at 37°C in a humidified atmosphere of 5% CO2 until 80% confluence ...
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bioRxiv - Cell Biology 2020Quote: ... The recombinant human EGF used in this study was from Thermo Fisher Scientific and the recombinant human HGF was generously provided by Drs ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... on plates coated with recombinant human collagen I (Coating Matrix kit, Gibco) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... and 5 ng/mL human recombinant GM-CSF (Thermo Fisher Scientific, USA) at 37 °C and 5 % carbon dioxide ...
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bioRxiv - Cancer Biology 2023Quote: ... human recombinant EGF (10 ng/ml) (Thermo Fisher Scientific, Waltham, MA, USA), D-glucose (5.5 mM ...
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bioRxiv - Cell Biology 2023Quote: ... Human recombinant plasminogen activator inhibitor 1 (PAI-1) was from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Bioengineering 2023Quote: ... and 5 ng/mL human recombinant GM-CSF (Thermo Fisher Scientific, USA). The incubator condition was set at 37 °C with 5 % carbon dioxide environment ...
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bioRxiv - Cell Biology 2023Quote: ... human recombinant fibroblast growth factor 2 (FGF2, 20 ng/mL, Life Technologies), and beta-mercaptoethanol (0.1% ...
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bioRxiv - Cancer Biology 2023Quote: ... bovine pituitary extract and human recombinant epidermal growth factor (Gibco, 37000-015)) and primary gingival keratinocyte (Dermal cell basal medium (ATCC ...
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bioRxiv - Cell Biology 2023Quote: ... hiPSCs were cultured on human recombinant vitronectin in StemFlexTM media (Life Technologies) and differentiation was initiated by plating singularised hiPSCs on human embryonic stem cells (hESC)-qualified Matrigel (Corning ...
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bioRxiv - Immunology 2023Quote: ... Recombinant human IFN-β (1000 U/ml to 1 U/ml, ThermoFisher) was used as positive controls for IRF activation ...
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bioRxiv - Biochemistry 2022Quote: ... recombinant human RPA or RPA-variants (2 μM) were incubated with 250 nM of recombinant Aurora B kinase (Invitrogen Inc.) in kinase reaction buffer (50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2020Quote: To compare the presence of the nucleolar rim of mNG-tagged proteins in live and fixed cells we fixed mNG-tagged cells in 4% formaldehyde (Thermo Scientific, 28908).
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bioRxiv - Bioengineering 2023Quote: ESCs were maintained on Recombinant Human Protein Vitronectin (Thermo Fischer # A14700) coated plates using mESC maintenance media containing Glasgow Minimum Essential Medium (Thermo Fisher Scientific # 11710035), Embryonic Stem Cell-Qualified Fetal Bovine Serum (Thermo Fisher Scientific # 10439001) ...
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bioRxiv - Cancer Biology 2021Quote: ... Biotinylated RNA baits were used in a ratio 1:12 to Hi-C libraries (25 ng biotinylated RNA baits per 300 ng of Hi-C library) and supplemented with 30 units SUPERase-In (Ambion). Biotinylated RNA baits for capture DNA-Seq were used in a ratio of 1:3.33 (300 ng RNA baits per 1,000 ng genomic DNA library ...
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bioRxiv - Biochemistry 2020Quote: ... Biotinylated proteins were enriched with Pierce™ streptavidin bead (Thermo Scientific™). The protein pellets were mixed to a pre-washed streptavidin beads (100 mM Hepes buffer) ...
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bioRxiv - Biochemistry 2021Quote: ... Biotinylated proteins were enriched by using Pierce Streptavidin Agarose beads (Thermo Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... Biotinylated proteins were affinity purified with magnetic streptavidin beads (Thermo Fisher Scientific) (40 μL slurry per sample ...
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bioRxiv - Cell Biology 2021Quote: ... Biotinylated proteins were enriched using Dynabeads™ M-280 Streptavidin (Invitrogen, 11205D) and eluted by heating the beads in 2X Laemmli buffer for immunoblotting.
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bioRxiv - Cell Biology 2020Quote: ... Biotinylated proteins were enriched on 100 μl streptavidin Ultralink beads (Thermo Scientific) for 1.5 h at room temperature with rotation ...
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bioRxiv - Cell Biology 2021Quote: ... Biotinylated proteins were purified on High Capacity Streptavidin Agarose beads (Thermo Scientific) in RIPA buffer and proteins were identified by mass spectrometry.
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bioRxiv - Microbiology 2020Quote: ... Biotinylated proteins were detected with Alexa-fluor-488-streptavidin (Molecular Probes S32354) and captured with Streptavidin High-Capacity Agarose beads (Thermo Scientific PI20359 ...
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bioRxiv - Plant Biology 2019Quote: ... Biotinylated proteins were detected with streptavidin-HRP (21124, Thermo Scientific, Rockford, IL).
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bioRxiv - Plant Biology 2019Quote: ... Biotinylated protein was detected with streptavidin-HRP (21124, Thermo Scientific, Rockford, IL).
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bioRxiv - Cell Biology 2022Quote: ... Biotinylated proteins were then incubated with streptavidin beads (MyOne Streptavidin C1, Invitrogen) for 1 h with rotation at +4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Biotinylated proteins were purified using 10 µl streptavidin-coupled magnetic beads (ThermoFisher) and eluted in 40 µl 1x Lämmli buffer at 70°C for 10min ...
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bioRxiv - Cell Biology 2023Quote: ... Biotinylated proteins were eluted in 1.5x NuPAGE LDS Sample buffer (Invitrogen #NP0007) containing 20 mM DDT (SRL #17315 ...
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bioRxiv - Cell Biology 2023Quote: ... Biotinylated proteins were eluted in 1.5x NuPAGE LDS Sample buffer (Invitrogen #NP0007) containing 20mM DDT (SRL #17315 ...
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bioRxiv - Plant Biology 2023Quote: ... The biotinylated protein extract was incubated with 100 μL Streptavidin resin (Invitrogen) for 60 min at 25°C with mild shaking in the dark ...