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Citations for Thermo Fisher :
551 - 600 of 10000+ citations for Recombinant Human PDCD1 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... synthesized GUN1-FL protein was purified using His-Tag Dynabeads™ (Invitrogen; 10103D) and confirmed by Western blotting using His-tag antibody (Sigma ...
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bioRxiv - Cell Biology 2020Quote: Recombinant 6XHis-tagged TbVps24 (N-terminus tag) was cloned using the pTrcHis TOPO TA expression system (Thermo Fisher Scientific, Carlsbad, CA) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: Human protein arrays (ProtoArrays V5.0, Life Technologies) kept at -20°C were equilibrated at 4 °C for 15 min and ...
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bioRxiv - Immunology 2020Quote: The assay to measure total IgG was modified to measure IgG subclass responses at 1/100 including a two-step biotinylated anti-human IgG subclass (mouse anti-human IgG1: HP6069, IgG2: HP6002, IgG3: HP6050 IgG4: HP6023, Thermo fisher Scientific, UK) with a streptavidin-Phycoerythrin tertiary (Thermo fisher Scientific ...
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bioRxiv - Genetics 2019Quote: ... Biotinylated surface proteins were recovered by incubating 200 μg of total protein with streptavidin-agarose beads (20347, Thermo Fisher) and eluting in Laemmli sample buffer ...
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bioRxiv - Immunology 2023Quote: ... Purified P.ch MSP1 protein and purified P.y MSP1 protein were biotinylated using the EZ-Link Sulfo-NHS-LC Biotinylation Kit (ThermoFisher) and tetramerized with streptavidin-PE (Agilent ...
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bioRxiv - Microbiology 2022Quote: ... A recombinant SPATR (rSPATR) fused to a polyhistidine tag (His-tag) was produced in BL21-CodonPlus-RIL competent cells (Invitrogen) and protein purification was performed under denaturing conditions using Ni-NTA Agarose (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... C-His-OipA protein was purified by using Dynabeads™ His-Tag Isolation and Pulldown magnetic bead system (Thermo Fisher Scientific, United States). His-tagged OipA protein isolation procedure was carried out according to kit instructions ...
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bioRxiv - Neuroscience 2021Quote: ... supplemented with 20 ng/mL human recombinant basic fibroblast growth factor (bFGF; Invitrogen, USA), 20 ng/mL epidermal growth factor (EGF ...
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bioRxiv - Microbiology 2019Quote: ... and 5 μg.L−1 of epidermal growth factor (EGF) human recombinant (Thermo Fisher Scientific) (Wu et al ...
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bioRxiv - Genetics 2019Quote: ... cells were incubated in differentiation medium (DM) (10 µg/ml recombinant human insulin (Gibco), 2% HI horse serum (Thermo Fisher) ...
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bioRxiv - Cell Biology 2021Quote: ... recombinant human insulin-like growth factor 1 (hIGF1; 100 ng/mL; Thermo Fisher Scientific), holo-transferrin (200 µg/mL ...
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bioRxiv - Cell Biology 2022Quote: ... Basal Medium plus 50 ng/ml human recombinant VEGFA (PHC9391 Gibco, Frederick, MD, USA) or Basal Medium plus 100 ng/ml recombinant human NTN1 (6419-N1 ...
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bioRxiv - Cell Biology 2022Quote: ... 4h prior treatment with recombinant human epidermal growth factor (EGF) (Cat# RP-10927, Invitrogen) at 5 ...
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bioRxiv - Neuroscience 2023Quote: ... and Alexa Fluor 647-conjugated recombinant human EGF (Invitrogen, #35351, Lot# 1700382, 1:1000) to label EGFR.
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bioRxiv - Immunology 2023Quote: ... supplemented with 294.4 ng/l human recombinant Epidermal Growth Factor (EGF) (Gibco, 10450-013), 25 mg/l Bovine Pituitary Extract (Gibco ...
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bioRxiv - Cell Biology 2023Quote: Recombinant tau monomers (human 4R2N T40/ Evotec, Germany) were labeled with Cy3 (Life Technologies). To prepare tau oligomers ...
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bioRxiv - Microbiology 2021Quote: The production of His-tagged soluble ACE2 was performed in FreeStyle™ 293-F cells and purified using HisPur™ Ni-NTA Resin (Thermo Scientific) as described previously [31].
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bioRxiv - Cell Biology 2021Quote: ... The second step was the separation of mCherry-zyxin or EGFP-VASP from 6xHis-MBP and the removal of the His-tagged TEV protease by Ni-NTA chromatography (HisPur™ Ni-NTA Resin, Thermo Scientific: 88222). First ...
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bioRxiv - Biophysics 2022Quote: ... anti-His tag antibody molecules were tagged with the dye Alexa Fluor® 647 NHS Ester (Succinimidyl Ester, ThermoFisher SCIENTIFIC, Catalog number: A20006) using the same procedure as followed in our previous paper.(11 ...
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bioRxiv - Cancer Biology 2019Quote: ... Biotinylated proteins were isolated by incubating the cell lysates with Avidin-coated beads (ThermoFisher) for 1h at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Biotinylated proteins were then purified using high capacity neutravidin-conjugated beads (Thermo Fisher Scientific) via incubation for three hours at 4°C after which beads were washed extensively in dilution buffer plus 0.5M NaCl ...
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bioRxiv - Immunology 2019Quote: ... Biotinylated proteins were coupled to FITC-labeled 1um FluoSpheres NeutrAvidin-labeled Microspheres (Thermo Fisher) at 1 ug to 1 ul ratio of protein to beads for 2 hours at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... RBD protein was first biotinylated with EZ-Link™ Sulfo-NHS-LC-Biotin (ThermoFisher) and then selected with streptavidin-coated magnetic Dynabeads™ M-280 (ThermoFisher) ...
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bioRxiv - Microbiology 2021Quote: ... RBD protein was first biotinylated with EZ-Link™ Sulfo-NHS-LC-Biotin (ThermoFisher) and then selected with streptavidin-coated magnetic Dynabeads™ M-280 (ThermoFisher) ...
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bioRxiv - Cell Biology 2020Quote: ... cell surface proteins were biotinylated using EZ-Link Sulfo-NHS-SS-Biotin (Thermo Fisher) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Biotinylated proteins were stained by incubating cells with Alexa Fluor 647-conjugated streptavidin (ThermoFisher). Coverslips were prepared for imaging by mounting in Vectashield liquid mounting media (Vector Labs).
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bioRxiv - Microbiology 2022Quote: ... Biotinylated proteins were pulled down with High Capacity NeutrAvidin-Agarose beads (Thermo Scientific, 29202) and analyzed by immunoblotting for S-nitrosylated ACE2 ...
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bioRxiv - Immunology 2022Quote: ... was biotinylated with the Biotin-XX Microscale Protein Labeling Kit (Life Technologies, NY, USA). 0.25 μg of biotinylated Ag or ~0.16 μg of BSA (used as a baseline control in an equivalent number of Ag molecules / bead ...
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bioRxiv - Plant Biology 2023Quote: ... and all biotinylated proteins were bound by PierceTM streptavidin agarose beads (Thermo Fisher Scientific). To this end ...
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bioRxiv - Cancer Biology 2023Quote: ... Vγ9Vδ2 TCR were biotinylated using protein labeling kit according to the manufacturer’s instructions (Thermofisher). Biotinylated proteins were then tetramerized with streptavidin-APC (Bio-legend ...
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bioRxiv - Microbiology 2023Quote: ... Biotinylated proteins were detected with streptavidin Alexa Fluor 546 conjugate (Molecular Probes; S-11225).
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bioRxiv - Immunology 2023Quote: ... which we biotinylated in-house using the Biotin-XX Microscale Protein Labeling Kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Biotinylated proteins were isolated from the extract using MyOne Streptavidin C1 Dynabeads (Thermo Scientific). To this end ...
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bioRxiv - Immunology 2022Quote: ... The spike protein was biotinylated using EZ-Link NHS-PEG4-Biotin reagent (Thermo Scientific) as follows ...
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bioRxiv - Systems Biology 2023Quote: The enrichment of biotinylated proteins was automated with the KingFisher Flex (Thermo Fisher Scientific). Supernatants were incubated at 4 °C for 18 hrs with magnetic streptavidin beads (Pierce™ Streptavidin Magnetic Beads ...
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bioRxiv - Cell Biology 2023Quote: ... Biotinylated proteins were detected in localization experiments using Streptavidin-Alexa674 (Life Technologies, 1:1200).
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bioRxiv - Cancer Biology 2024Quote: ... Cells were lysed and biotinylated proteins immunoprecipitated with streptavidin-conjugated agarose beads (ThermoFisher Scientific) overnight at 4°C with agitation ...
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bioRxiv - Molecular Biology 2021Quote: ... and Myc-tagged human Insig1 (NM_005542) were prepared by subcloning PCR products into the pcDNA3 vector (Invitrogen). YFP-tagged human RHBDL4 and YFP-human SREBP-1c-CFP were prepared by subcloning PCR products into the pCI-EYFP vector ...
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bioRxiv - Immunology 2019Quote: ... and counter-stained with fluorophore-tagged goat anti-rabbit or anti-human IgG (H+L) (Life Technologies) respectively ...
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bioRxiv - Biophysics 2023Quote: ... they were transfected with the S6-tagged human TrkA by means of Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer instructions ...
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bioRxiv - Neuroscience 2023Quote: ... VF-tagged human SYK and FCER1G plasmids for mammalian expression were generated using Gateway cloning technology (Invitrogen) as described previously (47) ...
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bioRxiv - Cell Biology 2020Quote: ... The lysate was then incubated with appropriate antibodies for 12 h and then conjugated with dynabeads Protein A or Protein G beads for an additional 1 h SFB- or GFP-tagged proteins were immunoprecipitated with streptavidin dynabeads (Invitrogen) or GFP-Trap (Chromotek) ...
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bioRxiv - Microbiology 2023Quote: ... Soluble proteins were purified with the PrepEase Histidine-tagged Protein Purification Midi Kit-High Yield (Affymetrix, Santa Clara, CA, USA) or Protino Ni-IDA 2000 packed columns (Macherey-Nagel ...
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bioRxiv - Biochemistry 2024Quote: ... Recombinant neuraminidase and recombinant hemagglutinin were biotinylated in a 20:1 molar ratio of biotin to antigen with EZ-Link NHS-Biotin (ThermoFisher, 20217) following the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... recombinant human IL-3 (R&D, Cat. 203-IL-050/CF, 25 ng mL-1) or c) human M-CSF recombinant protein (Invitrogen, Cat. PHC9501, 100 ng mL-1). A partial medium change was performed every 48 hours with 2x cytokine composites to replenish cytokines ...
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bioRxiv - Immunology 2021Quote: ... The protein was transiently expressed as a soluble recombinant protein in mammalian Expi293 cells (Thermo Fisher Scientific). The secreted protein self -assembles into a protein nanoparticle ...
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bioRxiv - Microbiology 2022Quote: ... The concentration of the purified recombinant proteins was determined by the BCA protein assay kit (Thermo Scientific) using bovine serum albumin (BSA ...
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bioRxiv - Immunology 2023Quote: ... The concentration of the purified recombinant proteins was determined by the BCA protein assay kit (Thermo Scientific) with bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2023Quote: ... All recombinant RBD-hFc proteins were purified using Pierce Protein A/G Plus Agarose (Thermo Scientific, 20424). In general ...