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Citations for Thermo Fisher :
651 - 700 of 10000+ citations for Recombinant Human CTRL Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... specimens were incubated with 10 nM purified recombinant POLArISact protein or 2-5 nM Alexa Fluor 488 Phalloidin (AF488-phalloidin, A12379, Thermo Fisher Scientific) and 10 mg/ml bovine serum albumin (BSA ...
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bioRxiv - Immunology 2020Quote: His-tagged recombinant mouse CD47 (as well as proteins and antibodies noted later) was labeled using AlexaFluor488 NHS Ester (Succinimidyl Ester, Thermo Fisher Scientific) reconstituted in anhydrous DMSO (dimethylsulfoxide ...
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bioRxiv - Immunology 2020Quote: ... Plates were then incubated with 1μg/ml of biotinylated recombinant ACE2 protein for 1 hour at room temperature followed by incubation with HRP-conjugated streptavidin (Thermo Fisher Scientific) for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... S1-RBD and ACE2 recombinant proteins were expressed in Expi293 cells for 5 days following transient transfection using Expifectamine 293 Transfection Kit (Thermo Fisher Scientific). Cell cultures were centrifuged at 4500g for 40 min ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.4 picomol of folded reporter mRNA was mixed with 20 picomol of recombinant protein and 200 picomol of UltraPure BSA (Thermo Fisher # AM2618) in binding buffer (10 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2022Quote: ... 28 fmol of folded reporter mRNA was mixed with 0-10 picomol of recombinant protein and 100 picomol of UltraPure BSA (Thermo Fisher # AM2618) on ice for 1 hr ...
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bioRxiv - Microbiology 2023Quote: Concentration of recombinant proteins were determined by measuring absorbance at 280 nm using a NanoDrop 1000 and/or NanoDrop One (Thermo Fisher Scientific) considering the extinction coefficient and the molecular weight calculated with the ProtParam tool (http://www.expasy.org/tools/protparam.html).
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bioRxiv - Immunology 2023Quote: ... the microtiter plates were washed 5 × with PBS-T and then incubated for 1 hour at room temperature with 2 μg/ml of biotinylated recombinant protein G (PierceTM, Thermo Fisher Scientific) for recognition of both bat and human IgG antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... Single guide RNAs (sgRNAs) were incubated together with recombinant Cas9 (TrueCut Cas9 Protein v2, 5ug/ul, Thermo Fisher Scientific, cat. no. A36499) for 15 min at room temperature ...
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bioRxiv - Genomics 2023Quote: ... Integrity of the GST tag was subsequently checked via western blotting of the purified recombinant protein using anti-GST antibodies (Invitrogen 71-7500) [1:1,500 dilution] ...
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bioRxiv - Cell Biology 2023Quote: ... and Alt-R® CRISPR-Cas9 tracrRNA Atto550 labeled, IDT) and recombinant Cas9 protein (Alt-R® S. p. HiFi Cas9 Nuclease V3, IDT) using Lipofectamine™ CRISPRMAX (Invitrogen) and cultured for 48 h in the presence of an HDR enhancer (final concentration 20 μM ...
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bioRxiv - Microbiology 2023Quote: ... AcGFP-JEVcore recombinant protein and Alexa Fluor 594 or 555-conjugated antibody (2 mg/mL, Thermo Fisher Scientific, as an injection marker) were microinjected into the cytoplasm at a final concentration of 1 mg/mL ...
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bioRxiv - Cell Biology 2024Quote: ... Expression of the recombinant LZTR1 proteins was confirmed by Western blot analysis using an anti-His tag monoclonal rabbit antibody (Thermo Fisher Scientific). Following confirmation of expression ...
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bioRxiv - Immunology 2019Quote: ... the wild-type and mutant (C302/305R) recombinant Mul1 proteins were produced in Sf9 cells using the Bac-to-Bac Baculovirus expression system (Invitrogen, Carlsbad, CA, USA) and purified by a nickel-chelating resin (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: In vitro binding experiments were performed with recombinant GST fusion proteins purified from E.coli and immobilized on glutathione-Sepharose 4B beads (Life Technologies #17-0756-01). Purification of GST fusion proteins was performed as described (Ebnet et al. ...
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bioRxiv - Neuroscience 2020Quote: ... The same recombinant Cdk5/p25 protein used for the MST assays was labeled with biotin using EZ-LinkNHS-PEG4-Biotinylation Kit (Thermo Scientific, Waltham, MA) and excess reagent was removed with a spin desalting column as per the manufacturer’s instruction ...
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bioRxiv - Immunology 2020Quote: The plasmid expressing the soluble pre-fusion version of SARS-CoV-2 S was used to produce the recombinant protein using the FreeStyle™ 293 Expression System (Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... A concentration range of indicated recombinant proteins was analyzed by thermal melt assays in SEC buffer using SYPRO Orange (Molecular Probes, cat.# S6651) using a Biorad CFX96 Touch Real-Time PCR C1000 System (ramping from 4 to 100°C at 1°C per minute).
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bioRxiv - Microbiology 2020Quote: ... recombinant proteins were purified by affinity chromatography as described previously (27) and loaded on NuPAGE 4-12% BisTris gels (Invitrogen, Carlsbad, CA, USA) to confirm the correct molecular weights and assess their purity.
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bioRxiv - Biochemistry 2024Quote: ... The recombinant SARS-CoV-2 Spike S1-hFc-His tagged protein used for titrations and sorting was purchased from ThermoFisher (RP-876-79). The recombinant neuraminidase (NA ...
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bioRxiv - Cell Biology 2020Quote: ... 50ng/mL recombinant murine EGF (Gibco, PMG8043), 100ng/mL recombinant human Noggin (Peprotech ...
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bioRxiv - Cell Biology 2020Quote: ... 50ng/ml recombinant EGF (Thermo Fisher Scientific), 10% conditioned medium from a cell line producing Noggin (kind gift from Hans Clevers) ...
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bioRxiv - Immunology 2022Quote: ... The recombinant ORF8 (ThermoFisher, Cat. RP-87666) was conjugated with FITC using the FITC conjugation kit (Abcam ...
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bioRxiv - Molecular Biology 2019Quote: ... and RNaseOUT recombinant ribonuclease inhibitor (Invitrogen, 10777019) was added to lysate (1ul per 100ul lysate) ...
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bioRxiv - Genomics 2019Quote: ... 0.25 μL RNaseOUT Recombinant Ribonuclease Inhibitor (Invitrogen), was then added to each well ...
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bioRxiv - Cell Biology 2021Quote: ... Recombinant Cas9 2.0 (Thermo Fisher Scientific, A36498) was mixed with gRNA and incubated for 15 min at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Recombinant pSinRep5 and helper plasmid pDH26S (Invitrogen) were transcribed in vitro into capped RNA using the Megascript SP6 kit (Ambion) ...
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bioRxiv - Microbiology 2022Quote: ... and 0.2 ng/mL recombinant EGF (Gibco). The transfection of primary NHKs to generate HPV18+ keratinocytes was performed as described previously [86] ...
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bioRxiv - Molecular Biology 2022Quote: ... RNaseOUTTM Recombinant Ribonuclease Inhibitor (Thermo Fisher Scientific) and Protoscript II reverse transcriptase (New England BioLabs ...
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bioRxiv - Physiology 2022Quote: ... Recombinant PIK3CG was purchased from Thermo Fisher.
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bioRxiv - Developmental Biology 2022Quote: ... Recombinant TrueCut Cas9 V2 (Thermo Fisher Scientific) was diluted in resuspension buffer R ...
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bioRxiv - Immunology 2024Quote: ... RNaseOUT™ Recombinant Ribonuclease Inhibitor (10777019, Invitrogen); Deoxynucleotide (dNTP ...
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bioRxiv - Cancer Biology 2024Quote: ... and 0.2 ng/mL recombinant EGF (Gibco). HPV18+ NHKs were generated as described previously [86] ...
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bioRxiv - Cell Biology 2024Quote: ... RNaseOUT™ Recombinant Ribonuclease Inhibitor (Thermo Fisher), Random Hexamer Primers (Thermo Fisher) ...
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bioRxiv - Immunology 2021Quote: ... Protein secretion levels in culture supernatant were quantified via ELISA for human IgG (Invitrogen, 50-112-8849) and human BAFF (R&D Systems ...
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bioRxiv - Genomics 2020Quote: ... Human PDCD1 or PDL1 cell surface protein staining was analyzed using anti-PD-1 (ThermoFisher clone MIH4) or anti-PD-L1 (ThermoFisher clone MIH1 ...
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bioRxiv - Cell Biology 2021Quote: Peak lists were searched against the complete human proteome (UniProt release 2014_08, with 68.049 proteins) using SEQUEST (Proteome Discoverer 1.3, ThermoFisher) as search engine ...
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bioRxiv - Molecular Biology 2021Quote: ... Then recombinant dsDNA fragment (add to > 400 ng/μl final concentration) or recombinant single-strand DNA (ordered from Invitrogen or IDT ...
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bioRxiv - Microbiology 2020Quote: ... the recombinant proteins were produced in their respective expression systems according to standard procedures and purified using either Ni-NTA (ThermoFisher Scientific, Waltham, MA, USA) or Strep-Tactin (IBA Lifesciences ...
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bioRxiv - Immunology 2021Quote: ... and 50ng purified viral RNA/DNA was incubated with the recombinant protein at different concentrations for 90min at 37°C in the presence of SUPERase In™ RNase inhibitor (Invitrogen, catalog no. AM2694), and the resulting RNA/DNA was tested for viral copies by qRT-PCR ...
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bioRxiv - Microbiology 2020Quote: ... followed by incubation with rabbit antibodies against SFTSV-N as primary antibodies and HRP-conjugated recombinant protein A/G (Cat. No. 32490, Thermo Scientific, Rockford, IL, USA) as secondary antibodies ...
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bioRxiv - Biochemistry 2023Quote: ... and the bound Arr3_ýC•IP6–Fab7 complexes incubated with HRP-conjugated Pierce recombinant protein L (cat: 32420, Thermofisher, 1:5000 dilution in ELISA buffer) for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... Cells were transfected with 1 μg/ml of recombinant Tat using a commercial transfection reagent Pro-ject Protein Transfection Reagent Kit (Thermo Scientific Cat. No. 89850) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... and the bound NTCP-Fab complexes were incubated with a secondary HRP-conjugated Pierce recombinant protein L (Thermofisher, 1:5000 dilution in ELISA buffer) for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... and human (goat anti-human IgG, ThermoFisher) were also used ...
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Targeted degradation of PCNA outperforms stoichiometric inhibition to result in programed cell deathbioRxiv - Cell Biology 2022Quote: Human embryonic kidney T-REx 293 cells which express the tetracycline repressor protein were purchased from Thermo Fisher Scientific and cultured in Eagle’s Minimum Essential Medium (MEM ...
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bioRxiv - Cell Biology 2022Quote: Human proteins (LIS1-SNAP, or motor domains) were expressed and purified from insect cells (ExpiSf9 cells; Life Technologies) as previously described with minor modifications3,7,20,69 ...
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bioRxiv - Biophysics 2023Quote: ... 77,897 human protein sequences) using the Sequest search engines within the Proteome Discoverer platform (version 2.4, Thermo Scientific). The following search parameters were used ...
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bioRxiv - Genetics 2021Quote: ... H3K4me1 Recombinant Polyclonal Antibody (Cat No. 710795, Invitrogen), normal rabbit and mouse IgG (Santa Cruz Biotechnology)
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bioRxiv - Immunology 2022Quote: ... and recombinant murine IL-18 (Thermo Fisher Scientific) at 1 ng/ml unless otherwise specified ...