Labshake search
Citations for Thermo Fisher :
451 - 500 of 10000+ citations for Recombinant H9N2 A Guinea fowl Hong Kong WF10 99 HA Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2019Quote: ... Biotinylated surface proteins were recovered by incubating 200 μg of total protein with streptavidin-agarose beads (20347, Thermo Fisher) and eluting in Laemmli sample buffer ...
-
bioRxiv - Immunology 2023Quote: ... Purified P.ch MSP1 protein and purified P.y MSP1 protein were biotinylated using the EZ-Link Sulfo-NHS-LC Biotinylation Kit (ThermoFisher) and tetramerized with streptavidin-PE (Agilent ...
-
bioRxiv - Immunology 2021Quote: ... HA proteins were expressed by transient transfection of 293F (ThermoFisher Scientific, R79007) or Expi293F (ThermoFisher Scientific ...
-
bioRxiv - Plant Biology 2023Quote: ... Total protein extracts were immunoprecipitated with anti-HA magnetic beads (Thermo Scientific); input and immunoprecipitation (IP ...
-
bioRxiv - Bioengineering 2020Quote: ... T cells were cultured in RPMI-1640 supplemented with 10% HI-FBS and fed with recombinant human IL-2 (ThermoFisher) and IL-15 (Miltenyi ...
-
bioRxiv - Microbiology 2022Quote: ... A recombinant SPATR (rSPATR) fused to a polyhistidine tag (His-tag) was produced in BL21-CodonPlus-RIL competent cells (Invitrogen) and protein purification was performed under denaturing conditions using Ni-NTA Agarose (Qiagen ...
-
bioRxiv - Systems Biology 2023Quote: ... T cells were expanded in RPMI-1640 media supplemented with 10% heat-inactivated FBS (HI-FBS) as well as recombinant human IL-2 (50 U/mL; ThermoFisher) and IL-15 (1 ng/mL ...
-
bioRxiv - Microbiology 2024Quote: ... C-His-OipA protein was purified by using Dynabeads™ His-Tag Isolation and Pulldown magnetic bead system (Thermo Fisher Scientific, United States). His-tagged OipA protein isolation procedure was carried out according to kit instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... The axonemal proteins were incubated overnight with anti-HA magnetic beads with bound anti-HA antibodies (Thermo Fisher Scientific, Waltham, MA) at 4 °C ...
-
bioRxiv - Microbiology 2021Quote: The production of His-tagged soluble ACE2 was performed in FreeStyle™ 293-F cells and purified using HisPur™ Ni-NTA Resin (Thermo Scientific) as described previously [31].
-
bioRxiv - Cell Biology 2021Quote: ... The second step was the separation of mCherry-zyxin or EGFP-VASP from 6xHis-MBP and the removal of the His-tagged TEV protease by Ni-NTA chromatography (HisPur™ Ni-NTA Resin, Thermo Scientific: 88222). First ...
-
bioRxiv - Biophysics 2022Quote: ... anti-His tag antibody molecules were tagged with the dye Alexa Fluor® 647 NHS Ester (Succinimidyl Ester, ThermoFisher SCIENTIFIC, Catalog number: A20006) using the same procedure as followed in our previous paper.(11 ...
-
bioRxiv - Neuroscience 2023Quote: ... SH-SY5Y cells were transfected with either an empty vector or with a vector expressing His-tagged human p35 (gift from Dr. Harish Pant) using Lipofectamine 3000 (Life Technologies, Carlsbad, CA). Two days after the transfection ...
-
bioRxiv - Cancer Biology 2019Quote: ... Biotinylated proteins were isolated by incubating the cell lysates with Avidin-coated beads (ThermoFisher) for 1h at 4°C ...
-
bioRxiv - Neuroscience 2020Quote: ... Biotinylated proteins were then purified using high capacity neutravidin-conjugated beads (Thermo Fisher Scientific) via incubation for three hours at 4°C after which beads were washed extensively in dilution buffer plus 0.5M NaCl ...
-
bioRxiv - Immunology 2019Quote: ... Biotinylated proteins were coupled to FITC-labeled 1um FluoSpheres NeutrAvidin-labeled Microspheres (Thermo Fisher) at 1 ug to 1 ul ratio of protein to beads for 2 hours at 37°C ...
-
bioRxiv - Microbiology 2020Quote: ... RBD protein was first biotinylated with EZ-Link™ Sulfo-NHS-LC-Biotin (ThermoFisher) and then selected with streptavidin-coated magnetic Dynabeads™ M-280 (ThermoFisher) ...
-
bioRxiv - Microbiology 2021Quote: ... RBD protein was first biotinylated with EZ-Link™ Sulfo-NHS-LC-Biotin (ThermoFisher) and then selected with streptavidin-coated magnetic Dynabeads™ M-280 (ThermoFisher) ...
-
bioRxiv - Cell Biology 2020Quote: ... cell surface proteins were biotinylated using EZ-Link Sulfo-NHS-SS-Biotin (Thermo Fisher) according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2022Quote: ... Biotinylated proteins were stained by incubating cells with Alexa Fluor 647-conjugated streptavidin (ThermoFisher). Coverslips were prepared for imaging by mounting in Vectashield liquid mounting media (Vector Labs).
-
bioRxiv - Microbiology 2022Quote: ... Biotinylated proteins were pulled down with High Capacity NeutrAvidin-Agarose beads (Thermo Scientific, 29202) and analyzed by immunoblotting for S-nitrosylated ACE2 ...
-
bioRxiv - Immunology 2022Quote: ... was biotinylated with the Biotin-XX Microscale Protein Labeling Kit (Life Technologies, NY, USA). 0.25 μg of biotinylated Ag or ~0.16 μg of BSA (used as a baseline control in an equivalent number of Ag molecules / bead ...
-
bioRxiv - Plant Biology 2023Quote: ... and all biotinylated proteins were bound by PierceTM streptavidin agarose beads (Thermo Fisher Scientific). To this end ...
-
bioRxiv - Immunology 2023Quote: ... which we biotinylated in-house using the Biotin-XX Microscale Protein Labeling Kit (Invitrogen) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... Biotinylated proteins were detected with streptavidin Alexa Fluor 546 conjugate (Molecular Probes; S-11225).
-
bioRxiv - Cell Biology 2022Quote: ... Biotinylated proteins were isolated from the extract using MyOne Streptavidin C1 Dynabeads (Thermo Scientific). To this end ...
-
bioRxiv - Immunology 2022Quote: ... The spike protein was biotinylated using EZ-Link NHS-PEG4-Biotin reagent (Thermo Scientific) as follows ...
-
bioRxiv - Cancer Biology 2023Quote: ... Vγ9Vδ2 TCR were biotinylated using protein labeling kit according to the manufacturer’s instructions (Thermofisher). Biotinylated proteins were then tetramerized with streptavidin-APC (Bio-legend ...
-
bioRxiv - Systems Biology 2023Quote: The enrichment of biotinylated proteins was automated with the KingFisher Flex (Thermo Fisher Scientific). Supernatants were incubated at 4 °C for 18 hrs with magnetic streptavidin beads (Pierce™ Streptavidin Magnetic Beads ...
-
bioRxiv - Cell Biology 2023Quote: ... Biotinylated proteins were detected in localization experiments using Streptavidin-Alexa674 (Life Technologies, 1:1200).
-
bioRxiv - Cancer Biology 2024Quote: ... Cells were lysed and biotinylated proteins immunoprecipitated with streptavidin-conjugated agarose beads (ThermoFisher Scientific) overnight at 4°C with agitation ...
-
bioRxiv - Microbiology 2022Quote: ... Cells were transfected with 2 μg of phCMV3 plasmids encoding the C-terminal HA-tagged NA from the strain of interest using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... The internalization of the ACE2-HA protein and anti-hACE2 mAbs was then evaluated by staining with goat anti-mouse Alexa Fluor™ 594 (to detect the HA-tagged hACE2) and/or goat anti-human Alexa Fluor™ 488 antibodies (to detect the hACE2 mAbs) (ThermoFisher Scientific). Images were captured using a DeltaVision OMX SR imaging system (GE Healthcare).
-
bioRxiv - Cell Biology 2023Quote: ... the cells were transfected with 1μg/well of the HA-tagged Fpn plasmid (Vector Builder, VB220407-1185gaa, Supplemental Figure 1) using Lipofectamine™ 3000 Transfection Reagent (Invitrogen, L3000001).
-
bioRxiv - Cell Biology 2020Quote: ... The lysate was then incubated with appropriate antibodies for 12 h and then conjugated with dynabeads Protein A or Protein G beads for an additional 1 h SFB- or GFP-tagged proteins were immunoprecipitated with streptavidin dynabeads (Invitrogen) or GFP-Trap (Chromotek) ...
-
bioRxiv - Microbiology 2023Quote: ... Soluble proteins were purified with the PrepEase Histidine-tagged Protein Purification Midi Kit-High Yield (Affymetrix, Santa Clara, CA, USA) or Protino Ni-IDA 2000 packed columns (Macherey-Nagel ...
-
bioRxiv - Biochemistry 2024Quote: ... Recombinant neuraminidase and recombinant hemagglutinin were biotinylated in a 20:1 molar ratio of biotin to antigen with EZ-Link NHS-Biotin (ThermoFisher, 20217) following the manufacturer’s instructions.
-
bioRxiv - Neuroscience 2022Quote: ... sodium borohydride (99%)—all from Acros Organics, Fair Lawn ...
-
bioRxiv - Cancer Biology 2021Quote: ... LysoTracker Red DND-99 (ThermoFisher Scientific, #L7528), anti-Rab5A (Cell Signaling Technologies ...
-
bioRxiv - Cell Biology 2021Quote: ... LysoTracker™ Red DND-99 (L7528, Invitrogen) and Magic-red (Immunochemistry Technologies ...
-
bioRxiv - Cell Biology 2021Quote: ... Lysotracker Red DND-99 (Thermo Fisher Scientific) was used at a final concentration of 50 nM and incubated for 40 min at 37°C before flow cytometry.
-
bioRxiv - Neuroscience 2021Quote: ... and formate ammonium (99%) from Acros Organics. The step gradient was ...
-
bioRxiv - Synthetic Biology 2021Quote: ... or n-decane (99+%, pure, Acros Organics). A mixture of 5.2 mL silicon oil (viscosity 5 cst (25 °C) ...
-
A Novel PHOX/CD38/MCOLN1/TFEB Axis Important For Macrophage Activation During Bacterial PhagocytosisbioRxiv - Immunology 2019Quote: ... LysoTracker Red DND-99 (Thermo Fisher, L7528) was added to the media 30 minutes before fixing according to the manufacture instruction ...
-
bioRxiv - Biochemistry 2021Quote: ... LysoTracker Red DND-99 (Thermo Fisher Scientific) was used for staining lysosomes ...
-
bioRxiv - Cancer Biology 2021Quote: ... LysoTracker Red DND-99 (Invitrogen Cat#: L7528) was resuspended as a 1 mM stock in DMSO ...
-
bioRxiv - Physiology 2022Quote: Rapamycin powder (>99%, #R1017-200mg, Fisher Scientific) was dissolved in the ethanol/tegosept solution [31] that is usually added to the cooling sugar agar medium before the plates are poured ...
-
bioRxiv - Developmental Biology 2023Quote: LysoTracker Red DND-99 (ThermoFisher Lot L7528) was diluted to 2μM in DMEM containing 50% rat serum ...
-
bioRxiv - Synthetic Biology 2023Quote: ... and LysoTracker Red DND-99 (ThermoFisher, L7528) was added to visualize nuclei and lysosomes ...
-
bioRxiv - Cell Biology 2023Quote: ... Lysotracker-red DND 99 (Thermo Scientific, L7528) and Hoescht (Abcam ...