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Citations for Thermo Fisher :
701 - 750 of 10000+ citations for Recombinant H9N2 A Guinea fowl Hong Kong WF10 99 HA Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... Proteins were extracted and purified using Pierce Anti-HA magnetic beads (ThermoFisher Catalog number 88836) for 3 hours ...
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bioRxiv - Plant Biology 2023Quote: ... The remainder was incubated with equilibrated anti-HA coupled Protein G magnetic beads (12CA5, Thermofisher) for 2.5 hours at 4°C on a rotator ...
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bioRxiv - Molecular Biology 2020Quote: ... Biotinylated target proteins were purified with 25µL (slurry) of MyONE-C1 streptavidin beads (Thermo Fisher Scientific, 65002), pre-washed with 1x NLB + 1x PhosSTOP ...
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bioRxiv - Bioengineering 2020Quote: ... Detection of EDA positive fibronectin was performed using biotinylated fusion proteins followed by Streptavidin Alexa 488 (Invitrogen). In parallel the neovasculature marker CD31 was detected using either rat anti-mouse CD31 (BD Pharmingen ...
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bioRxiv - Neuroscience 2020Quote: Biotinylated cell-surface proteins were pulled down with streptavidin-coupled magnetic beads (#11205D; Thermo Fisher Scientific, US). The beads were isolated ...
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bioRxiv - Microbiology 2019Quote: ... Cells were lysed with RIPA buffer and biotinylated proteins were precipitated with streptavidin-agarose beads (Thermo Fisher). Elution was carried out with Laemmli buffer (2% (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... the biotinylated proteins were purified by incubating the lysates with High Capacity Streptavidin Agarose beads (Pierce, ThermoFisher) overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Biotinylated proteins were detected with streptavidin coupled to Alexa Fluor 488 or 594 (1:1000; Life Technologies).
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bioRxiv - Immunology 2022Quote: ... purified HIVJR-CSF gp120 protein was biotinylated using EZ-Link Sulfo-NHS-LC-Biotin (Thermo Fisher Scientific) and excess biotin was removed using a Zeba spin desalting column (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... N-terminally biotinylated 67B89 target protein was immobilized on Streptavidin Dynabeads M-270 (Invitrogen, Carlsbad, CA, USA). The target-coated beads were blocked with BSA and incubated with a suspension of 3.4·1012 phages in the presence of a 10-fold molar excess of the off-target (variant 6789) ...
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bioRxiv - Cell Biology 2023Quote: ... Cell surface proteins were biotinylated with 0.5mg/ml EZ-link-Sulfo-NHS-SS-Biotin (Thermo Fisher, A39258) in a PBS buffer with 2.5mM CaCl2 and 1mM MgCl2 on ice for 30 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... and the biotinylated cell surface proteins were affinity-purified on streptavidin magnetic beads (Thermo Scientific, cat#88816). After stripping off the nonspecifically bound proteins by several rounds of washing with the lysis buffer ...
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bioRxiv - Neuroscience 2023Quote: ... biotinylated protein signal was detected by incubation with streptavidin conjugated with Alexa Fluor 568 (Invitrogen, Cat# S11226) or 647 (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... and surface proteins were biotinylated with 0.5 mg/ml Premium Grade Sulfo-NHS-LC-Biotin (Fisher Scientific) in PBS at 4°C for 30 min ...
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bioRxiv - Developmental Biology 2019Quote: ... 633-goat α-guinea pig (Life Technologies).
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bioRxiv - Genetics 2020Quote: ... rabbit anti-guinea pig (ThermoFisher PA1-28595).
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bioRxiv - Neuroscience 2020Quote: ... 596 Goat anti-guinea-pig antibody (Invitrogen), and 647 Goat anti-rabbit antibody (Life Technologies).
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bioRxiv - Neuroscience 2022Quote: ... 488 goat anti-guinea pig (Invitrogen #A11073), 488 goat anti-mouse (Invitrogen #A11001) ...
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bioRxiv - Neuroscience 2022Quote: ... or anti-guinea pig A647 (A21450, Invitrogen) in CTA ...
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bioRxiv - Neuroscience 2023Quote: ... goat anti-guinea pig (1:500; Invitrogen); donkey antimouse (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... donkey anti-guinea pig Cy3 (ThermoFisher Scientific), donkey anti-rabbit Alexa Fluor 647 (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2019Quote: ... anti V5-HRP conjugate (to detect mSCARLET and other V5 tagged fluorescent proteins, catalog number 46-0708, Invitrogen), rabbit polyclonal anti TAL1 IgG (catalog number A305-300A ...
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bioRxiv - Cell Biology 2021Quote: ... 10 μg of GST tagged protein was conjugated to Pierce™ Glutathione Magnetic Agarose Beads (Thermo Fisher, 78602), for 1hr at RT ...
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bioRxiv - Biochemistry 2021Quote: ... 10 mM maltose.) SKD3-MBP fusion protein was cleaved with a 6His-tagged AcTEV™ protease (Thermo Fisher) at 4 °C for 96 h in the amylose elution buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... GST-tagged bait proteins were immobilized in a 96-well Flat bottom Nunc MaxiSorp plates (Nunc, Roskilde, Denmark) at 4°C for 18 hours (10 μg per protein in 100 μl PBS) ...
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bioRxiv - Cell Biology 2020Quote: ... cleared lysates were mixed with Protein G/A (50:50) recombinant protein G agarose beads (catalog #15920-010, Invitrogen, Carlsbad, CA) coupled with anti-HA (catalog #3724S ...
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bioRxiv - Microbiology 2019Quote: ... The cell lysate was incubated with superparamagnetic beads covalently linked to recombinant protein G (Dynabeads protein G Novex, Life technologies, USA) to obtain the precleared lysate ...
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bioRxiv - Cell Biology 2024Quote: SK-Mel28 cells were grown on coverslips and transiently transfected with ribonucleprotein complexes consisting of purified recombinant Cas9 protein (Truecut Cas9 Protein v2, Invitrogen, #36499) and synthetic guide RNAs (Invitrogen TrueGuide Synthetic sgRNA #35514 or Negative Control ...
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bioRxiv - Systems Biology 2021Quote: ... Tn5 underwent a His-tag (Dynabeads His-Tag Isolation & Pulldown, Invitrogen, 10103D) clean-up according to the manufacturer’s protocol to remove unbound oligos before using.
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bioRxiv - Immunology 2019Quote: ... The protein was expressed in Sf9 insect cells using a recombinant baculovirus system (Life Technologies). The protein was purified on a cobalt resin column and further purified by gel filtration to ensure the removal of monomeric and aggregated protein.
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bioRxiv - Biochemistry 2020Quote: ... The recombinant PfAdoMetDC protein was purified using the Strep-Tactin Sepharose column (Invitrogen, California, USA) as described previously (29 ...
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bioRxiv - Cell Biology 2022Quote: ... were combined with 0.36 μg of recombinant human protein CDK1/Cyclin B (Thermo Fisher, PV3292), 1x Kinase Buffer (Cell Signaling ...
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bioRxiv - Immunology 2021Quote: ... Concentration of purified recombinant Abs were determined by PierceTM BCA Protein Assay Kit (ThermoFisher Scientific) and the purity was assessed by sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE).
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bioRxiv - Immunology 2020Quote: ... Recombinant SARS-CoV-2 RBD protein was coupled to fluorescent beads (Thermo Scientific, Cat # F8819) by carbodiimide coupling ...
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bioRxiv - Cell Biology 2021Quote: Binding of recombinant proteins to pre-spotted PIP and membrane lipid strips (Thermo Fisher; P23751) was performed according to (Dowler et al. ...
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bioRxiv - Immunology 2021Quote: For competitive ELISA recombinant hACE2 protein were immobilized (400 ng per well) on immunoplates (Nunc MaxiSorp ...
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bioRxiv - Immunology 2020Quote: ... 100uL of recombinant protein at 0.5 ug/ml was coated on Maxisorp™ plates (Nunc) and incubated overnight at 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... the medium was supplemented with Human Interferon-gamma Recombinant Protein (IFN-γ) (ThermoFisher, Cat. #. PHC4031) at a concentration of 50 ng/mL ...
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bioRxiv - Cell Biology 2022Quote: ... the recombinant ACE2-Fc protein which was obtained from ExpiCHO™ expression system (Thermo Fisher) was 5-fold serially diluted in DMEM medium supplementing 10% FBS (Gibco) ...
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bioRxiv - Cancer Biology 2022Quote: ... Integrin-β2 recombinant protein complexes were immobilized on Maxisorp Immuno plates (ThermoFisher, 12-565-135) and used for positive binding selections with library phage pools that were first exposed to neutravidin coated wells to deplete nonspecific binders ...
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bioRxiv - Molecular Biology 2022Quote: ... Recombinant protein concentration was determined by Pierce Detergent Compatible Bradford Assay Kit (Thermo Fisher # 23246) with BSA standards diluted in Protein Storage Buffer before aliquoting in single use volumes ...
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bioRxiv - Bioengineering 2023Quote: ... Hu5F9 and TJC4 were then purified using recombinant Protein A-Sepharose 4B (Thermo Fisher Scientific) buffer exchanged into PBS and concentrated using Amicon Centrifugal Filters (Millipore Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... Recombinant protein concentration was determined by Pierce Detergent Compatible Bradford Assay Kit (Thermo Fisher # 23246) with BSA standards diluted in Protein Storage Buffer before aliquoting in single use volumes ...
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bioRxiv - Biochemistry 2023Quote: ... Recombinant protein concentration was determined by Pierce Detergent Compatible Bradford Assay Kit (Thermo Fisher # 23246) with BSA standards diluted in Protein Storage Buffer before aliquoting in single use volumes ...
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bioRxiv - Biochemistry 2023Quote: ... Recombinant protein concentration was determined by Pierce Detergent Compatible Bradford Assay Kit (Thermo Fisher # 23246) with BSA standards diluted in Protein Storage Buffer before aliquoting in single use volumes ...
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bioRxiv - Cell Biology 2023Quote: Reconstitute Human FGF-basic (FGF-2/bFGF) Recombinant Protein (here we use ThermoFisher 13256-029). Briefly ...
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bioRxiv - Biochemistry 2023Quote: ... Recombinant protein concentration was determined by Pierce Detergent Compatible Bradford Assay Kit (Thermo Fisher # 23246) with BSA standards diluted in Protein Storage Buffer before aliquoting in single use volumes ...
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bioRxiv - Cell Biology 2023Quote: Recombinant ectodomain proteins were generated by transfection of HEK293T cell cultures using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... then recombinant protein purified from the soluble fraction using HisPur Ni-NTA columns (Thermo Scientific). Purified proteins were dialyzed against EMSA buffer ...
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bioRxiv - Immunology 2023Quote: ... ELISAs were conducted with recombinant full proteins coated overnight in Nunc MaxiSorp 384 plates (ThermoFisher) (2µg/ml ...