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Citations for Thermo Fisher :
201 - 250 of 10000+ citations for Recombinant E. coli NAGK Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... recombinant proteins were detected using primary anti-His antibody (ThermoFisher, Cat. # PA1-983B) and an anti-RNAP antibody (BioLegend ...
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bioRxiv - Microbiology 2023Quote: ... Secondary horseradish peroxidase (HRP)-conjugated recombinant A/G protein (Invitrogen, lot number XH346151) diluted 1:20,000 was used for detection and visualized with KPL TMB two-component peroxidase substrate kit (SeraCare ...
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bioRxiv - Physiology 2023Quote: ... Recombinant proteins were produced in suspension culture in Freestyle 293F cells (Life Technologies) transiently transfected using FectroPro reagent (Polyplus) ...
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bioRxiv - Cell Biology 2023Quote: ... all iPSCs were cultured in Vitronectin Recombinant Human Protein (VTN-N) (ThermoFisher, A14700) pre-coated 6-well plates with 2 mL TeSR-E8 medium (Life Technologies ...
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bioRxiv - Immunology 2024Quote: Recombinant proteins were coated on Immulon 4 HBX 96-well plates (Thermo Scientific) at a concentration of 2 μg/mL in PBS ...
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bioRxiv - Microbiology 2024Quote: Recombinant Bd37 (rBd37) protein was expressed as thioredoxin fusion protein from the pBAD/Thio-TOPO vector (Thermo Fisher Scientific), which encodes thioredoxin and histidine-tag at N-terminus and C-terminus of multi cloning site ...
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bioRxiv - Immunology 2022Quote: Preparation of CD5L-coated pHrodo particles: one vial containing 2 mg of particles (pHrodo™ Red E. coli BioParticles™ Conjugate for Phagocytosis, ThermoFisher) were reconstituted with 500 μL of TBS with 5 mM CaCL2 and sonicated for 5 min ...
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bioRxiv - Biochemistry 2023Quote: ... Digested scFv inserts were subcloned in pET22b(+) vector (EMD Biosciences) and transformed into BL21(DE3) E-coli strain (Thermo Fisher Scientific, USA). Amber stop codon in CDR regions of scFvs were mutated to glutamine by site-directed mutagenesis kit (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... cDNAs from pUC57 entry vector were transformed by electroporation into Escherichia coli strain DH10B and transferred by a recombinant LR reaction of Gateway cloning (Clonase II enzyme mix Kit, Thermo Fisher) into pB7WGF2 (Karimi et al. ...
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bioRxiv - Microbiology 2019Quote: ... coli protein production was performed using the pET-SUMO (Thermo Fisher, Waltham, MA, USA) expression system in E ...
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bioRxiv - Molecular Biology 2019Quote: 6xHis 12-heptad SRS fusion proteins were expressed in SoluBL21 Escherichia coli cells (Invitrogen) from a pet42a vector ...
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bioRxiv - Evolutionary Biology 2022Quote: For protein purification Escherichia coli strains were tested for optimal expression: BL21 DE3 (Thermofisher) was selected for PpMurE_L63_pPROEX and BL21(DE3) ...
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bioRxiv - Cell Biology 2021Quote: ... coli (K-12 strain), and Staphylococcus aureus (Wood strain, without protein A) (Molecular Probes), were washed 3 times with PBS by centrifugation and sonicated for 3 times at 50 KHz for 20 s ...
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bioRxiv - Bioengineering 2020Quote: ... Gibco™ FGF-Basic AA 1-155 recombinant human protein (bFGF, PHG0264, Fisher Scientific), endothelial cell basal medium 2 (EGM 2 ...
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bioRxiv - Biophysics 2021Quote: ... The recombinant protein was expressed in Expi 293 GnTI- (Thermo Fisher, Waltham, MA, USA) at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... or by tryptic shaving of cell-surface proteins by using recombinant enzymes (TrypLE, Gibco). Three biological replicates of cells cultured in the presence or absence of serum ...
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bioRxiv - Immunology 2019Quote: ... Recombinant proteins were biotinylated with EZ-Link Sulfo NHS-LC-LC biotin (Thermo Fisher) at a 50 molar excess for 30 minutes at room temperature ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were cultured on 6-well plates coated with Vitronectin Recombinant Human Protein (Invitrogen) at a concentration of 0.5 µg/cm2 ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant Tau proteins were biotinylated with 5 molar excess of NHS-biotin conjugates (Thermofisher) during 4 hours at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... recombinant hACE2-Fc protein was first biotinylated using EZ-Link Sulfo-NHS-Biotin (ThermoFisher) as the instruction described ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant RBD proteins were produced in transfected FreeStyle 293F cells (Invitrogen, Carlsbad, CA, USA) and purified by nickel affinity chromatography ...
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bioRxiv - Biochemistry 2020Quote: ... recombinant N proteins were coated on 96 well flatbottom immunosorbent plates (Nunc Immuno MaxiSorp) at a concentration of 500 ng/mL ...
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bioRxiv - Cell Biology 2022Quote: Purified recombinant proteins were labelled using amine reactive NHS ester dyes (Thermo Fisher Scientific). Alexa fluorophores used to label proteins include ...
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bioRxiv - Immunology 2022Quote: Recombinant proteins were produced using the Freestyle™ 293 Expression System (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... All the recombinant proteins were transformed and expressed in E.coli BL21 (DE3) (Invitrogen, USA).
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bioRxiv - Microbiology 2023Quote: ... Recombinant BA.5 and BQ.1.1 RBD proteins were produced in Expi293F cells (ThermoFisher) by transfection of DNA using the ExpiFectamine 293 Transfection Kit (ThermoFisher) ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant protein was resolved by NuPAGE™ 4-12% Bis-Tris Gel (Invitrogen; NP0322BOX) to determine identity and purity ...
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bioRxiv - Biochemistry 2023Quote: The following recombinant proteins were purchased from commercial sources: alkaline phosphatase (Thermo Scientific #EF0651), Antarctic phosphatase (New England BioLabs ...
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bioRxiv - Immunology 2023Quote: ... and recombinant Cas9 protein via electroporation using the Neon transfection system (Thermo Fisher Scientific). The following day ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant proteins were purified using HisPur™ Ni-NTA resin (Thermo Fisher Scientific) and amylose resin (NEB) ...
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bioRxiv - Microbiology 2023Quote: ... and STRN3 were carried out utilising immobilised recombinant protein A/G resin (Thermo Scientific). Cell pellets were harvested and processed as described in the EGFP co-immunoprecipitation section ...
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bioRxiv - Biophysics 2021Quote: ... The SNAP-tag allows for covalent binding of O6-benzylguanine (BG) to the protein.[63] Protein expression was performed in Escherichia coli BL21(DE3) pRARE (Invitrogen) under T7 promoter with 1 mM isopropyl-β-D-thiogalactopyranoside (IPTG ...
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bioRxiv - Synthetic Biology 2022Quote: ... Cloning inserts were created via PCR of ORFs of interest from their respective genomic (all E. coli genes) or codon-optimized (all other genes) DNA with Phusion polymerase (Thermo Scientific, Waltham, MA). The resulting In-Fusion products were used to transform E ...
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bioRxiv - Neuroscience 2020Quote: ... cells were washed with PBS and incubated with pHrodo-labeled bioparticles (pHrodo™ Green E. coli BioParticles™ Conjugate for Phagocytosis, ThermoFisher Scientific) in OptiMem for three hours at +37°C ...
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bioRxiv - Microbiology 2020Quote: ... according to manufacturer’s instructions with primers purchased from Eurofins Genomics. E. coli DH5α and Stbl2 (Trinh et al., 1994, p. 2) (Thermo Fisher Scientific, USA) were used for amplification of standard plasmids or LTR containing plasmids ...
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bioRxiv - Bioengineering 2022Quote: ... bacteria were captured using commercially available beads with their associated magnetic separator device (anti-E. coli O157 Dynabeads®, DynaMag™ -2 magnet, Life Technologies) following the manufacturer’s guidelines for use ...
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bioRxiv - Plant Biology 2020Quote: ... coli (Invitrogen). TFs cloned in pDEST22 were thus expressed in yeast as C-terminal fusions to GAL4_AD ...
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bioRxiv - Cancer Biology 2021Quote: ... coli (Invitrogen™/Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... coli (Invitrogen). Blue/White positive selection was used to pick clones and recovered plasmids were sent for sequencing ...
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bioRxiv - Microbiology 2020Quote: ... coli (Invitrogen) were transformed by pLVX-puro-TK following manufacture’s guidelines ...
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bioRxiv - Cell Biology 2019Quote: ... coli (Invitrogen) were used for cloning pLVX-puro plasmids ...
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bioRxiv - Molecular Biology 2019Quote: ... coli (Affymetrix), chicken (BioChain) ...
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bioRxiv - Microbiology 2019Quote: ... coli (Invitrogen). Single colonies for each point mutant were Miniprepped (Qiagen ...
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bioRxiv - Microbiology 2019Quote: ... coli (Invitrogen) were subsequently transformed and plated to obtain single colonies of successfully assembled pGRA1-ROP17-3xHA plasmid ...
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bioRxiv - Immunology 2020Quote: ... coli (Invitrogen).
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bioRxiv - Molecular Biology 2019Quote: ... coli (Thermofisher) and 20 colonies were sequenced via Sanger sequencing using universal primer M13R.
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bioRxiv - Cell Biology 2019Quote: ... coli (Invitrogen). E ...
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bioRxiv - Cell Biology 2020Quote: ... coli (ThermoFisher) were grown aerobically at 37° in Luria-Broth medium.
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bioRxiv - Biochemistry 2021Quote: ... coli (Invitrogen) and grown in 50 mL of Luria Broth containing 50 μg/mL of kanamycin and grown at 37°C with shaking overnight ...
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bioRxiv - Bioengineering 2021Quote: ... coli (ThermoFisher) and selected on Luria-Bertani (LB ...