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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Recombinant E. coli NAGK Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: All recombinant proteins were expressed in Escherichia coli BL21 DE3 (Invitrogen), in LB medium ...
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bioRxiv - Biophysics 2022Quote: ... Escherichia coli (E. coli) BL21-AI cells (Invitrogen) were transformed with a modified pET vector encoding N-terminal 10X His tagged VcINDY ...
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bioRxiv - Bioengineering 2019Quote: ... fluorescent Escherichia coli (E. coli) BioParticles (ThermoFisher, cat. #E2863), or fluorescent carboxylate-modified 1 µm polystyrene beads (Invitrogen ...
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bioRxiv - Synthetic Biology 2023Quote: All recombinant proteins were produced in Escherichia coli BL21(DE3) pLysS (Invitrogen) transformed with respective plasmids ...
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bioRxiv - Microbiology 2024Quote: Glycerol stock of Escherichia coli (E. coli) (TOP10 strain, Invitrogen, Thermo Fisher Science ...
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bioRxiv - Biochemistry 2022Quote: All recombinant proteins were produced in Rosetta2 Escherichia coli competent cells (Thermo Fisher) induced with 0.4 mM isopropyl-β-d-1-thiogalactoside (IPTG ...
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bioRxiv - Biochemistry 2021Quote: ... and TnsB (WP_084763316.1) proteins were codon optimized for heterologous expression in Escherichia coli (E. coli) and synthesized by GeneArt (Thermo Fisher Scientific). The ShCas12k gene was inserted into the 1B (Addgene 29653 ...
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bioRxiv - Microbiology 2019Quote: ... Strains used for cloning and expression of recombinant proteins were Escherichia coli TOP10 (Invitrogen) and E ...
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bioRxiv - Molecular Biology 2024Quote: ... coli poly(A) polymerase (E-PAP, Invitrogen) at 37°C for 30 min in 50 µL reactions ...
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bioRxiv - Microbiology 2024Quote: ... To transform Stbl3 E coli cells (Invitrogen) with the BAC construct ...
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bioRxiv - Biochemistry 2022Quote: ... and Escherichia coli S15 (AP009048.1) proteins were codon optimized for heterologous expression in Escherichia coli (E. coli) and synthesized by GeneArt (Thermo Fisher Scientific). The ShCas12k gene was inserted into the 1B (Addgene 29653 ...
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bioRxiv - Immunology 2020Quote: Recombinant soluble ZIKV E protein was biotinylated and coupled to AF488 Neutravidin beads (Life Technologies). Microscale-purified mAbs were tested at a single 1:10 dilution ...
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bioRxiv - Immunology 2020Quote: Recombinant soluble ZIKV E protein was biotinylated and coupled to AF488 Neutravidin beads (Life Technologies). Microscale-purified mAbs were tested at a single 1:10 dilution ...
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bioRxiv - Microbiology 2019Quote: ... USA. Escherichia coli DH5α (hereafter called E. coli) was purchased from Invitrogen Inc. ...
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bioRxiv - Microbiology 2023Quote: ... Escherichia coli strain DH5α (E. coli DH5α, Thermo Fisher Scientific, Cat#18258012), E ...
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bioRxiv - Cell Biology 2020Quote: Plasmids encoding for recombinant His-tagged proteins were transformed into Escherichia coli BL21(DE3) (Invitrogen). Bacterial cultures were grown at 37°C in LB media to OD600 of 0.6-0.8 and induced with 0.5 mM IPTG at 18 °C for 16h (overnight) ...
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bioRxiv - Cell Biology 2019Quote: ... coli-derived recombinant human insulin (Gibco, A11382IJ), 64 μg ml−1 L -ascorbic acid 2-phosphate trisodium salt (Wako ...
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bioRxiv - Cell Biology 2023Quote: ... coli-derived recombinant human insulin (Gibco, A11382IJ). Contracting cells were noted from day 8 ...
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bioRxiv - Immunology 2020Quote: Recombinant soluble ZIKV E protein was biotinylated and coupled to red fluorescent Neutravidin beads (Life Technologies). Antibodies were diluted to 5 μg/mL in RPMI-1640 ...
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bioRxiv - Microbiology 2023Quote: ... kanamycin (Fisher Scientific; 40 µg/mL for E. coli), gentamicin (Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... kanamycin (Fisher Scientific; 40 µg/mL for E. coli), gentamicin (Fisher Scientific ...
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bioRxiv - Biophysics 2020Quote: CLGltPh and double cysteine mutants were transformed into Escherichia coli (E. coli) Top10 cells (Invitrogen). Bacterial cells were inoculated in Luria Broth medium containing 100 μg/mL ampicillin at 37 °C until OD600 ~0.6-0.8 ...
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bioRxiv - Neuroscience 2020Quote: ... and 0.09 μL Ambion RNase H (E. coli) (Invitrogen, AM2293). Second strand synthesis was performed by incubating samples at 16°C for 150 min ...
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bioRxiv - Genomics 2019Quote: ... 1 µl of 2 U/µl E Coli RNaseH (Invitrogen) and then incubating at 16°C for 2 hours ...
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bioRxiv - Developmental Biology 2021Quote: Escherichia coli (E. coli) strain DH5α (Toyobo, Osaka, Japan) and BL21(de3) pLysS (C606003, ThermoFisher Scientific) were used for DNA cloning and protein expression ...
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bioRxiv - Genomics 2022Quote: ... vector and amplified in DH5α strain of Escherichia coli (E coli) (Life Technologies, Grand Island NY). After amplification ...
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bioRxiv - Developmental Biology 2021Quote: Escherichia coli (E. coli) strain DH5α (Toyobo, Osaka, Japan) and BL21(de3) pLysS (C606003, ThermoFisher Scientific) were used for DNA cloning and protein expression ...
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bioRxiv - Microbiology 2024Quote: Glycerol stock of Escherichia coli (E. coli) (TOP10 strain, Invitrogen, Thermo Fisher Science, Waltham, MA, USA) transformed with plasmid pCR2.1 (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... were expressed in the bacterial Escherichia coli (E. coli) BL21(DE3) pLysS strain (Invitrogen, Thermo Fisher Scientific) as described in 38 ...
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bioRxiv - Biochemistry 2020Quote: ... were expressed in the bacterial Escherichia coli (E. coli) BL21(DE3) pLysS strain (Invitrogen, Thermo Fisher Scientific) as described in 38 ...
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bioRxiv - Immunology 2022Quote: ... Recombinant E protein was produced by transient transfection of Expi293F cells using a ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific). Cell supernatants were harvested 4 days after transfection and then concentrated before exchange into 2× PBS at pH 6.5 and finally into 2× PBS at pH 8.0 ...
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bioRxiv - Immunology 2020Quote: ... 20⧅l of opsonised Escherichia coli (E.coli) was added to the tube (pHrodo Red E. coli BioParticles; ThermoFisher). The bacteria was initially prepared by mixing the E.coli strain with new-born calf sera followed by a 30-minute incubation at 37°C ...
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bioRxiv - Biophysics 2024Quote: ... The recombinant DR2539 was expressed in Escherichia coli BL21 (DE3) (Invitrogen). The cells were grown at 310 K to an OD600 of ≈ 0.6 in Luria-Bertani medium containing 100 µg ml-1 of ampicillin ...
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bioRxiv - Systems Biology 2024Quote: ... EGF Recombinant Human Protein (Gibco #PHG0313), FGF Basic (aa 10 155 ...
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bioRxiv - Biochemistry 2020Quote: ... All the SAM with natural isotope abundance and 2H or 13C enrichment were enzymatically synthesized from L-methionine and ATP with appropriate isotope labels. Escherichia coli (E. coli) DH5α and BL21(DE3) competent cells were from Invitrogen. Data plotting and nonlinear least-squares fitting was carried out using GraphPad Prism 7 software ...
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bioRxiv - Immunology 2019Quote: ... or pH sensitive fluorescent bioparticles (pHrodo E. coli Bioparticles Conjugate for Phagocytosis; Invitrogen). Each fly was injected with 27.6-32.2 nl of bioparticles (1mg/100µl ...
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bioRxiv - Biochemistry 2019Quote: ... Filtrate was added onto DNA Polymerase 1 (PolI) (E. coli source, Fisher Scientific), 0.25U/uL reaction ...
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bioRxiv - Biophysics 2024Quote: ... Ligated vectors were transformed into competent bacteria (One Shot TOP10 E. coli; Invitrogen) and grown overnight on ampicillin plates ...
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bioRxiv - Cell Biology 2022Quote: ... DNA polymerases and NEBuilder HiFi DNA assembly master mix were obtained from New England Biolabs. Cloning was performed in TOP10 chemically competent Escherichia coli (E. coli) (ThermoFisher Scientific). Buffers for assessing fluorescence lifetime were composed of either 0.01M PBS (pH 6.5 – 7.4 ...
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bioRxiv - Cell Biology 2022Quote: ... All synthetic oligonucleotides were obtained from Integrated DNA technologies (IDT). Cloning was performed in TOP10 chemically competent Escherichia coli (E. coli) (ThermoFisher Scientific). mApple and TfR DNA was obtained from mApple-Lysosomes-20 (RRID:Addgene_54921 ...
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bioRxiv - Biophysics 2022Quote: ... novicida U112 (Fn)Cas12a (WP_003040289) was codon optimized for heterologous expression in Escherichia coli (E. coli) and synthesized by GeneArt (Thermo Fisher Scientific). The FnCas12a gene was inserted into the 1B plasmid (Addgene #29653 ...
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bioRxiv - Neuroscience 2023Quote: ... 15 μg/mL and 50 000 cells/mL respectively. Bioparticles of pHrodoTM Green-labelled Escherichia coli (E. coli) were purchased from Thermo Fisher Scientific and used at a concentration of 25 μg/mL ...
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bioRxiv - Bioengineering 2020Quote: ... 1.61 nM EGF Recombinant Human Protein (Invitrogen), 0.178 mM Adenine (Sigma) ...
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bioRxiv - Neuroscience 2021Quote: ... Recombinant LRRK2 protein was from ThermoFisher (#A15197). For pT73 Rab10 assay ...
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bioRxiv - Developmental Biology 2021Quote: ... G-CSF Recombinant Human Protein (Thermo fisher), IP injection ...
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bioRxiv - Microbiology 2020Quote: ... coli-derived recombinant BAG1) and secondary goat anti-rabbit 594 (1:1000, Invitrogen).
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bioRxiv - Microbiology 2022Quote: ... the recombinant 3CLpros were expressed in Escherichia coli BL21 cells (Invitrogen, Carlsbad, CA) grown in Luria Bertani broth induced with 1mM isopropyl ß-D-thiogalactopyranoside ...
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bioRxiv - Neuroscience 2020Quote: ... plasmids were transformed into chemocompetent bacteria (E. coli Top10™, Thermo Fisher Scientific, TFS), which were generated like previously described70 ...
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bioRxiv - Bioengineering 2023Quote: MMP9-pro-cat-Hiswt (E coli expression) was expressed in BL21(DE3) (Thermo Fisher). Cells were grown in 2xYT at 37°C and expression was induced with 0.5 mM isopropyl ?-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Biophysics 2020Quote: The plasmid pET3a containing human AβM42 and AβMC40 cDNA was transformed into Escherichia coli (E. coli) One Shot® BL21 (DE3) pLysS (Thermo Fisher Scientific, USA). The plasmids were a kind gift from Prof ...