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Citations for Thermo Fisher :
151 - 200 of 10000+ citations for Puumala Virus Glycoprotein 2 Gc Human Heterodimeric Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: Human fibroblast cells were reprogrammed using the Sendai virus-based CytoTune iPS 2.0 kit (CytoTune 2.0, Invitrogen, A16517). Fibroblasts were plated in six-well plates two days before transduction to achieve a density of 2 × 105 to 3 × 105 cells per well ...
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bioRxiv - Cancer Biology 2021Quote: Single live cell suspensions were washed with 1% FCS in PBS and resuspended in flow cytometry buffer (2% FCS and 1 mM ethylenediaminetetraacetic acid (EDTA; Thermo Fisher #AM9260G) in PBS).
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bioRxiv - Microbiology 2022Quote: Expression plasmids encoding CLII or CLIV with an Fc- and His6-tag were transfected in Expi293F expression cells (Gibco-Thermo Fischer Scientific) using the ExpiFectamine293 transfection kit ...
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bioRxiv - Immunology 2020Quote: Recombinant SARS-CoV-2 S-RBD fused with Fc/His tag (S-RBD-His) was produced in 293T cells and purified with Protein A Agarose (Thermo Fisher Scientific). Recombinant human ACE2-ECD fused with Flag/His tag was produced in 293T cells and purified with anti-DYKDDDDK G1 Affinity Resin according to the manufacturer’s instructions (GenScript).
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bioRxiv - Biochemistry 2019Quote: ... The cells were then incubated using FITC-labelled goat-anti-human IgG-FC antibody (ThermoFisher Scientific) (200 ng/ul ...
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bioRxiv - Immunology 2019Quote: ... cells were stained with a PE-conjugated anti-human IgG1(Fc) secondary antibody (1:50) (ThermoFisher) for additional 15min ...
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bioRxiv - Microbiology 2021Quote: ... R-phycoerythrin (PE) conjugated goat anti-human IgG Fc secondary antibody (Invitrogen, Cat#12-4998-82) and PE conjugated mouse anti-PSGL-1 mAb (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... and 1:5,000 diluted goat anti-human-IgG Fc horseradish peroxidase (HRP) conjugated secondary antibodies (Invitrogen) were added ...
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bioRxiv - Cancer Biology 2022Quote: ... competition was detected by HRP-goat anti-human IgG Fc secondary antibody (A18817, Thermo Fisher Scientific). The kinetics of the antibody fragments were determined using a Biacore X100 (GE Healthcare) ...
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bioRxiv - Bioengineering 2023Quote: ... binding was quantified by measuring the fluorescence of a PE-conjugated anti-human Fc antibody (Invitrogen) detecting the Fc-fused protein target ...
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bioRxiv - Bioengineering 2023Quote: ... HRP-conjugated goat anti-human IgG-Fc (10000x dilution) was used for detection (Invitrogen Cat# 31413). Immobilon Western Chemiluminescent HRP Substrate (Millipore Sigma ...
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bioRxiv - Microbiology 2019Quote: ... CR-Fc or DC-SIGN-Fc (Thermofisher) in binding buffer (BB ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Derivatized samples were analyzed using a GC-MS (Trace 1310 GC, Thermo Fisher Scientific, Waltham, MA, USA) coupled to a MS detector system (ISQ QD ...
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bioRxiv - Genetics 2020Quote: ... with a final quench in PBS + 2% fetal calf serum (FCS, Invitrogen 26140079). Cells were permeabilized for 5 minutes with saponin buffer (PBS + 0.2% Saponin (Sigma S7900 ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 15% fetal calf serum (FCS) and 2 mM Gln (ThermoFisher Scientific) at 37°C in 1% CO2 ...
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bioRxiv - Immunology 2022Quote: ... 10 μg/ml in FACS-buffer (PBS, 2% fetal calf serum [FCS, Gibco Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... 24 h later the dish was washed with PBS and treated with PR8 virus (MOI =2) in the infection medium [F-12K with 2% BSA fraction V (Thermo Fisher), 1% antibiotic-antimycotic (Thermo Fisher) ...
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bioRxiv - Biochemistry 2020Quote: ... Nanobody binding was detected using Myc-tag specific antibody (2 μg/ml – Life Technologies) and then anti-mouse-Alexa Fluor 700 (1.3 μg/ml – Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were probed with monoclonal HA Tag antibody (1:2000 dilution, Invitrogen, 2-2.2.14,), incubated with anti-mouse IgG-horseradish peroxidase (IgG-HRP ...
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bioRxiv - Biophysics 2020Quote: ... PCoV_GX and RaTG13 pseudoviruses were generated by co-transfection of human immunodeficiency virus backbones expressing firefly luciferase (pNL43R-E-luciferase) and pcDNA3.1 (Invitrogen) expression vectors encoding the respective spike protein into 293T cells (ATCC) ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV and MERS-CoV pseudovirus were generated by co-transfection of human immunodeficiency virus backbones expressing firefly luciferase (pNL43R-E-luciferase) and pcDNA3.1 (Invitrogen) expression vectors encoding the respective S proteins into 293T cells (ATCC ...
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bioRxiv - Immunology 2022Quote: ... medium supplemented with 2% human AB serum (Gibco). The concentration of IL-2 was increased to 1000 IU/ml on day 7 and the expanded T cells were electroporated with the indicated mRNA at a concentration of 2 pg mRNA/cell on day 8 using 4D Nucleofector™ System (Lonza ...
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bioRxiv - Immunology 2021Quote: ... Recombinant human IL-2 was purchased from ThermoFisher Scientific.
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bioRxiv - Immunology 2023Quote: ... goat-anti human IgG F(ab’)2 (Invitrogen) was covalently coupled to yellow-green carboxylate beads (Thermofisher) ...
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bioRxiv - Cancer Biology 2023Quote: ... Human Beta-2-Microglobulin (B2M) (ThermoFisher, Cat# 4333766T) served as an endogenous control for RNA input ...
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bioRxiv - Systems Biology 2021Quote: ... Aroma compounds were separated by GC in a Thermo TRACE GC ULTRA chromatograph (Thermo Fisher Scientific, Waltham, MA) equipped with a flame ionization detector (FID) ...
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bioRxiv - Systems Biology 2021Quote: ... Aroma compounds were separated by GC in a Thermo TRACE GC ULTRA chromatograph (Thermo Fisher Scientific, Waltham, MA) equipped with a flame ionization detector (FID) ...
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bioRxiv - Biochemistry 2021Quote: FAME were analyzed by Gas Chromatography-Flame Ionisation Detection (GC-FID) on a Trace GC Ultra (ThermoFisher Scientific) equipped with a biscyanopropyl polysiloxane column (Restek ...
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bioRxiv - Neuroscience 2023Quote: GC-MS data was recorded with Single Quadrupole Mass Spectrometer and GC (Trace 1310) (Thermo Fisher Scientific, USA). The column was 30 m long dimethyl (95%)/diphenyl polysiloxane (5% ...
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bioRxiv - Microbiology 2021Quote: ... Concentrated virus stocks in reduced-serum DMEM (2% FBS) supplemented with 50mM HEPES buffer (Gibco #15630080) were stored at -80°C ...
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bioRxiv - Biochemistry 2021Quote: ... supplemented with 10% FCS (FCS, Thermo Fisher Scientific), 100 U ml−1 penicillin ...
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bioRxiv - Cell Biology 2021Quote: ... GCs were cultured in RPMI-1640 (Gibco) supplemented with 10% FBS (Natocor) ...
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bioRxiv - Molecular Biology 2020Quote: ... Medium GC-content non-silencing siRNA (Invitrogen) was transfected as a negative control ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The GC/MS (Thermo Scientific Trace Ultra) was equipped with a single quadrapole mass spectrometer (Thermo Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... The GC/MS (Thermo Scientific Trace Ultra) was equipped with a single quadrupole mass spectrometer (Thermo Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... 2 μg of hK14-LaNto31-T2A-mCherry or LaNt-α31-pSec-Tag and 2 μl Lipofectamine 2000 (Thermo Fisher Scientific) were used ...
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bioRxiv - Biochemistry 2021Quote: Four rounds of panning were used to isolate scFvs binding both MERS-CoV S2 and SARS-CoV-2 spike using the following solutions coated on high binding plates: 2 μg/mL anti-c-myc tag antibody (Invitrogen) to eliminate phage expressing no or truncated scFv (Round 1) ...
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The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... filtered through a 70 µm cell strainer, washed with FACS buffer (PBS supplemented with 2% FCS, 2 mM EDTA and 0.01% Sodium Azide (all Gibco)) and maintained at 4°C ...
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bioRxiv - Immunology 2021Quote: ... the concentrations of human IL-2 and IFN-γ were measured using a IL-2 Human Uncoated ELISA Kit (Thermo Fisher) and IFN-γ Human Uncoated ELISA Kit (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... V5 tag to detect CBUA0028 or the Xpress tag (Invitrogen) to detect CBU0665.
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bioRxiv - Microbiology 2022Quote: ... V5 tag to detect CBUA0028 or the Xpress tag (Invitrogen) to detect CBU0665.
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bioRxiv - Immunology 2024Quote: ... the blots were probed for the presence of heavy and light chain bands using either horseradish peroxidase (HRP)-conjugated polyclonal goat anti-human IgG Fc antibody or polyclonal goat anti-human kappa chain antibody (Thermo Fisher Scientific, Waltham, MA, USA). Antibody-coupled HRP activity was detected with Supersignal West Dura Extended Duration Substrate (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 2% v/v heat-inactivated FCS and sodium bicarbonate (Gibco 25080-060). Diluted compounds were then mixed with EGFP-expressing Vero E6 cells at 25,000 cells/well in 96-well plates (Greiner Bio-One ...
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bioRxiv - Cell Biology 2021Quote: ... HA-tag (Invitrogen), Flag-tag (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... isotope enrichment analysis in TCA cycle metabolites were determined using GC-MS (Q-Exactive GC-Orbitrap, Thermo Fisher Scientific). For this purpose metabolites were derivatized using a two-step procedure starting with an methoxyamination (methoxyamine hydrochlorid ...
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bioRxiv - Immunology 2021Quote: ... IL-2 Human Uncoated ELISA kit and IFNγ Human Uncoated ELISA kit (both from Invitrogen) and DuoSet Human IFN-gamma kit and Ancillary Reagent Kit 2 (R&D Systems ...
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bioRxiv - Genomics 2021Quote: ... cells or virus pellet were incubated with 2 mM of EZ-Link Psoralen-PEG3-Biotin (ThermoFisher Scientific) at 37°C for 10 min in PBS containing 0.01% digitonin ...
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bioRxiv - Microbiology 2021Quote: The spike of the SARS-CoV-2 virus (D614G variant) was generated (GeneArt Gene Synthesis, ThermoFisher Scientific) from the codon-optimized sequence obtained by Ou et al ...
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bioRxiv - Microbiology 2023Quote: ... The BA.1 and BA.2 supernatants were additionally concentrated using the Intact Virus Precipitation Reagent (Invitrogen). All viral stocks were stored at -80°C until use ...
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bioRxiv - Immunology 2020Quote: ... 2 μl mouse anti-human CD14 APC (Invitrogen, MHCD1405), and 20 μg of human IgG were added to glass dishes containing PBMCs ...