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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Puumala Virus Glycoprotein 2 Gc Human Heterodimeric Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Strep-tag (NBP2-41073; Novusbio) or influenza virus hemagglutinin tag (2-2.2.14; Thermo Scientific). After washing with PBS with 1% BSA ...
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bioRxiv - Cancer Biology 2023Quote: ... Vesicular stomatitis virus glycoprotein-pseudotyped virus was produced by co-transfecting 293T cells using Lipofectamine 2000 (Invitrogen) with an shRNA transducing vector and 2 packaging vectors ...
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bioRxiv - Immunology 2020Quote: ... or residues 319–591 of SARS-CoV-2 S with a C-terminal monomeric human IgG Fc-tag and an 8x HisTag (SARS-CoV-2 RBD-SD1) were transiently transfected into FreeStyle293F cells (Thermo Fisher) using polyethylenimine ...
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bioRxiv - Molecular Biology 2021Quote: The coding sequence of MHV-S Hemagglutinin Esterase inactive (S45A) (1218bp) fused to human IgG-Fc followed by a 6xHis tag in the mammalian expression vector pcDNA3.1(-) (Invitrogen) was kindly provided by Colin Parrish (MHV-S-HE_Fc_His6 ...
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bioRxiv - Immunology 2022Quote: ... 2% FCS (ThermoFisher), and 50 μg/mL gentamicin (ThermoFisher) ...
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bioRxiv - Immunology 2023Quote: ... 2% FCS (Gibco), 25 mM HEPES (Merck ...
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bioRxiv - Molecular Biology 2020Quote: Mammalian expression plasmids encoding SARS VHH72 and MERS VHH-55 (73) with a C-terminal a monomeric human Fc tag were transfected into ExpiCHO cells (Thermofisher) using ExpiFectamine as manufacterer’s recommendation ...
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bioRxiv - Neuroscience 2020Quote: ... β-neurexin-1 extracellular domain was cloned into the pCAGGS vector with a C-terminal human Fc tag and the ATD regions of GluA1–4 were cloned into a modified pDisplay vector (Invitrogen), as described previously (9) ...
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bioRxiv - Molecular Biology 2021Quote: ... and PToV Hemagglutinin Esterase non-binding (F271A) (1176bp) fused to human IgG-Fc followed by a 6xHis tag in the Insect expression vector pFastBac1 (ThermoFisher) was kindly provided by Colin Parrish (PToV-HE_S46A-Fc_Hisx6/pFastBac1 ...
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bioRxiv - Immunology 2021Quote: ... residues 306–577 of MERS-CoV S with a C-terminal monomeric human IgG Fc-tag and an 8x HisTag (SARS-CoV RBD-SD1) were transiently transfected into FreeStyle293F cells (Thermo Fisher) using polyethylenimine ...
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bioRxiv - Bioengineering 2022Quote: ... Mouse IgG2a Fc Tag (Acro Biosystems) incubation followed with APC Goat anti-mouse IgG2a Fc Antibody (Invitrogen) staining and RFP expression ...
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bioRxiv - Cell Biology 2021Quote: ... and 4 μg of plasmid VSV-G encoding G glycoprotein of vesicular stomatitis virus(VSVG) using Lipofectamine 3000 Reagent (Invitrogen). 12 h later ...
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bioRxiv - Cancer Biology 2022Quote: Glycoproteins were isolated using a glycoprotein isolation kit (89804; Thermo Fisher Scientific) as per the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: ... Goat anti-human Fc-HRP conjugate (Invitrogen) was used at a 1:10,000 dilution in blocking buffer for 1 h at RT ...
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bioRxiv - Microbiology 2020Quote: SADS-CoV spike glycoprotein (virus strain GDS04; GenBank No.: ASK51717.1) gene was synthesized with codons optimized and inserted into pFastBac vector (Life Technologies Inc.). The ectodomain of SADS-CoV spike protein without the transmembrane anchor and intracellular tail (residues 18-1068 ...
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bioRxiv - Immunology 2020Quote: ... Ebola virus Makona Glycoprotein (EBOV-GP) (KM233102.1) was synthesized and cloned into pcDNA3.1 by GeneArt AG technology (Life Technologies, Regensburg, Germany) or VSV-G following the manufacturer’s instructions of Lipofectamine 3000 (Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... HEK293T cells were co-transfected with the pNL4.3.Luc.R-E-viral vector as well as a plasmid expressing the vesicular stomatitis virus glycoprotein (VSV-G) envelope (pMD2.G) using Lipofectamine 3000 (Life Technologies) according to basic manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... plates were washed and incubated for one hour with SULFO-TAG conjugated anti-human IgG for mAbs (MSD) or SULFO-TAG conjugated polyclonal anti-human IgG+A+M (Thermo Fisher) for serum samples ...
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bioRxiv - Systems Biology 2021Quote: ... anti-human FITC-IgG (Fc specific) (ThermoFisher Scientific) at a dilution of 1:400 and for 45 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... 100ug/mL anti-human IgG(Fc) (Life Technologies) was added to virus-Ab complexes and incubated for 30 min at 37°C prior to the addition of cells as described in (39) ...
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bioRxiv - Cancer Biology 2020Quote: ... VHS40789) and a nonspecific control siRNA duplex with similar GC content (siCtrl; Medium GC Duplex #2) from Invitrogen. siRNAs against MCM5 were designed according to a previous report (Ge et al. ...
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bioRxiv - Immunology 2020Quote: ... and F(ab’)2-Goat anti-Human IgG-Fc secondary antibody conjugated with R-phycoerythrin (ThermoFisher Catalog # H10104). Labeled cells were acquired using a Thermo-Fisher Attune NxT flow cytometer and analyzed using Flowjo Software.
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bioRxiv - Biochemistry 2022Quote: ... was then used for glycoprotein purification using both ConA and WGA Glycoprotein Isolation Kits (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... were transfected with each plasmid expressing SPΔC of SARS-CoV-2 Delta variant (1.2 µg DNA) or human influenza A virus M2 (5 µg DNA) using Lipofectamine 3000 (Invitrogen, USA) to produce 293TN-S target cells ...
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bioRxiv - Biochemistry 2021Quote: ... The SARS-CoV-2 spike glycoprotein RBD was expressed in Freestyle 293 cells (ThermoFisher, MA) grown in Freestyle 293 media at 37 °C with 8% CO2 and shaking at 135 rpm in a humidified incubator ...
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bioRxiv - Biochemistry 2023Quote: ... Glycoproteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP-HCl; Thermo Scientific) and alkylated with 10 mM 2-Chloroacetamide (Sigma-Aldrich ...
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bioRxiv - Biophysics 2020Quote: ... and inserted into a human Fc expression vector (Invitrogen). The proteins were expressed in HEK293T cells ...
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bioRxiv - Immunology 2022Quote: ... glycoproteins were digested with trypsin (ThermoFisher) overnight at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... and Pierce Glycoprotein Staining kit (ThermoFisher) were used for protein and glycan staining ...
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bioRxiv - Biochemistry 2022Quote: ... Histidine rich glycoprotein (HRG) was purified from human plasma with a cobalt-loaded resin (Thermo Scientific, Waltham, America) using IMAC-based enrichment (56) ...
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bioRxiv - Biochemistry 2021Quote: ... while the other was stained for glycoproteins using the Pro-Q Emerald 300 glycoprotein staining kit (Invitrogen) following manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... 2 µl of SuperFi GC enhancer (Thermo Fisher Scientific, Vilnius, Lithuania), 1 mmol/l each of reverse and forward verification primers (Supplementary Table S1) ...
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bioRxiv - Cell Biology 2024Quote: ... Stealth RNAi siRNA Negative Control Med GC Duplex #2 (Thermofisher Scientific) was used.
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bioRxiv - Cell Biology 2023Quote: ... 2 mM Glutamax and 10% FCS (Invitrogen) in humid environment containing 5% CO2.
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 2% FCS (Thermo Fisher Scientific), 1% penicillin/streptomycin ...
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bioRxiv - Cancer Biology 2023Quote: ... Human OTUD4 constructs were cloned without tag or with FLAG-tag into the expression plasmid pcDNA3.1 (Life technologies) using the oligos forward (BamHI ...
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bioRxiv - Biophysics 2021Quote: ... and both HRP-conjugated anti-mouse IgG Fc and anti-human IgG Fc were purchased from Invitrogen™ (Waltham ...
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bioRxiv - Microbiology 2021Quote: ... FXa-HRP conjugated anti-human Fc antibody (05-4220, Invitrogen) was used as a detecting antibody ...
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bioRxiv - Immunology 2022Quote: ... or IgG1 Fc (human) protein (Thermo Fisher Scientific, Rockford, IL) was added to a final concentration of 10 mg/mL and samples were incubated with gentle mixing for 2 hours at room temperature ...
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bioRxiv - Immunology 2021Quote: ... then incubated with human Fc receptor coupled to phycoerythrin (ThermoFisher). To set positive gates ...
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bioRxiv - Immunology 2021Quote: ... or specific for the Fc region of human IgG (Invitrogen), the Fc region of human IgM (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Microbiology 2021Quote: ... or specific for the Fc region of human IgG (Invitrogen), the Fc region of human IgM (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Genetics 2022Quote: ... Tissues were incubated with mouse anti-P-glycoprotein C219 antibody at 3.6μg/mL (Glycoprotein Monoclonal Antibody C219, Cat. No MA1-26528, Invitrogen) overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... A glycoprotein ladder (Candy Cane ladder; Invitrogen) served as positive control ...
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bioRxiv - Microbiology 2024Quote: ... along with a CandyCane glycoprotein ladder (Thermofisher). We additionally loaded the same two B ...
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bioRxiv - Immunology 2023Quote: ASCs (FVS780-CD20-CD3-CD14-CD16-CD19+CD27hiCD38hi) were sort-purified from human peripheral blood mononuclear cells into culture media (10% FCS, 2 mM L-glutamine (Gibco), 100 units/mL Penicillin and 100 ng/mL Streptomycin (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... Stealth RNAi Negative Control Medium GC Duplex #2 (Invitrogen, Cat. #12935-112) was used.
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bioRxiv - Cell Biology 2020Quote: ... washed with 2 % FCS/PBS and then re-suspended in 2 % FCS/PBS containing 7-amino actinomycin (7-AAD, Invitrogen) at a concentration of 5 µg/ml ...
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bioRxiv - Developmental Biology 2022Quote: ... HBSS with 2 % FCS (Gibco, cat no. 10500064) was used for the whole procedure ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 2% FCS (ThermoFisher, cat no A4766801) containing doubling dilutions of the various compounds at the stated concentrations ...