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Citations for Thermo Fisher :
351 - 400 of 4418 citations for PhotoCol Methacrylated Type I Collagen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... or rat tail collagen 1 (GIBCO). Cells were then treated as indicated and images were recorded with an Olympus IX71 microscope with 60x oil objective (Olympus) ...
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bioRxiv - Cell Biology 2022Quote: ... precoated with collagen (rat tail, Gibco) for 24 h before the isolation.
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bioRxiv - Cell Biology 2023Quote: ... or rat tail collagen 1 (GIBCO). Cells were then treated as indicated and images were recorded with an Olympus IX71 microscope with 60x oil objective (Olympus) ...
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bioRxiv - Neuroscience 2023Quote: ... and collagen (Thermo Fisher Scientific A1048301). Astrocytes serve as a feeder layer for neurons and were seeded at 50,000/well one week before hippocampal neuronal culture ...
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bioRxiv - Molecular Biology 2024Quote: ... and 80% rat tail collagen (Gibco) for 2 hours at 37 prior to seeding with freshly isolated murine AT2s in 10% DMEM overnight ...
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bioRxiv - Cell Biology 2020Quote: ... a 4 hour enzymatic digestion into single cells was performed at 37°C in 35 mls HBSS containing 5 mg/ml collagenase type I (Life Technologies), 10 μM ROCK inhibitor Y-27632 (StemRD) ...
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bioRxiv - Bioengineering 2023Quote: ... The dermal layers of the skin were separated using forceps and further subjected to incubation with type I collagenase (1 mg/ml Gibco, USA) for 2 hours at 37 degrees ...
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bioRxiv - Cell Biology 2023Quote: ... and the EDL was immediately incubated in plating medium (DMEM + 10% horse serum + 2% Penicillin/Streptomycin + 1% HEPES) containing type I collagenase (2.6 mg/mL; Gibco, 17100-017) in a 37°C shaking water bath for 53 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... Blood free perfused lungs were digested in Phosphate Buffered Saline (Mg and Ca free) with Collagenase Type I (Gibco Cat# 17100017), DNase (Millipore Sigma Cat# D5025) ...
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bioRxiv - Cell Biology 2020Quote: Control or various KD or KO cells were seeded on collagen I coated (at final 50 μg/ml concentration, Thermo Fisher Scientific, Grand Island, NY) glass coverslips ...
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bioRxiv - Cell Biology 2022Quote: Cells were seeded into a multi-layer system of collagen (rat-tail collagen used throughout; ThermoFisher # A1048301) and fibrin as shown in Fig ...
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bioRxiv - Cancer Biology 2020Quote: ... The tissue homogenate was incubated in 10 ml digesting buffer containing 0.5 mg ml-1 collagenase type I (ThermoFisher, catalogue #17100-017) in Advanced DMEM/F12 ...
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bioRxiv - Immunology 2022Quote: ... The dermis part was cut into 1 mm width pieces and mixed with mixed enzyme solution containing type I collagenase (Gibco, Grand Island) and trypsin (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... cut into half and measured as two technical replicates) were digested with 0.2% collagenase type II and 0.05% hyaluronidase in reduced serum OptiMEM-I (Gibco-BRL, Grand Island, NY) media supplemented with 50 µg/mL of gentamycin for 3h at 37°C with frequent agitation on a tube rotator (model ...
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bioRxiv - Cell Biology 2020Quote: ... cut into 1 × 1 cm pieces and incubated overnight in digestion medium containing 1 mg/ml collagenase type I (Gibco, Massachusetts, USA) and 100 μl/ml antibiotic/antimycotic solution in Modified Hank’s Balanced Salt Solution (HBSS ...
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bioRxiv - Immunology 2022Quote: Serum collected from mice were analyzed for type I IFNa and IFNb using Mouse IFN ProQuantum Immunoassay Kit (Invitrogen #A46736 and #A47435), following manufacture’s protocols.
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bioRxiv - Molecular Biology 2023Quote: ... Blood free perfused lungs were digested in Phosphate Buffered Saline (Mg and Ca free) with 2 mg/ml Collagenase Type I (Gibco Cat 17100017) and 50 units of DNase (Millipore Sigma Cat D5025) ...
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bioRxiv - Microbiology 2024Quote: ... coli type I fimbria or FliC (diluted 1:10 000 in protein-free tris-buffered saline (TBS) blocking solution (Fisher Scientific, France)) for 1 h at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Opti-MEM I - Glutamax I (Gibco) supplemented with 2% FBS and 50 µg/ml Gentamicin (GIBCO ...
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bioRxiv - Cancer Biology 2021Quote: ... Acid-extracted rat tail collagen (Gibco, A1048301) was neutralized on ice in the presence of 10X DMEM with 0.1N NaOH such that the final concentration of the collagen is 1 mg/mL ...
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bioRxiv - Systems Biology 2020Quote: We used collagen rat tail 1 (Gibco) 150 μg/ml for coating nano-patterned substrate (width ...
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bioRxiv - Microbiology 2021Quote: ... pre-coated with collagen (Thermo Fisher #A1064401) according to the manufacturer protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... Acid-extracted rat tail collagen (Gibco, A1048301) was neutralized on ice in the presence of 10X DMEM with 0.1N NaOH such that the final concentration of the collagen is 1 mg/mL ...
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bioRxiv - Bioengineering 2022Quote: ... or collagen X (14-9771-82; Invitrogen) then visualized using a mouse and rabbit specific HRP/DAB (ABC ...
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bioRxiv - Cell Biology 2022Quote: ... DQ Collagen was purchased from Thermo Fisher Scientific (D12060) ...
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bioRxiv - Bioengineering 2024Quote: ... were coated with collagen (Gibco, #A10483-01) and stored at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... Type IV (ThermoFisher) for 1 hr and dissociation into smaller pieces through manual tritulation before seeding onto feeder layer of MEFs.
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bioRxiv - Developmental Biology 2020Quote: ... The seminiferous tubules were digested in 5 mL of DPBS solution I consisting of 1 mg/mL collagenase type IV (17104019, Gibco, Grand Island, NY) and 20 U/mL DNase I (2212 ...
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bioRxiv - Cancer Biology 2020Quote: ... The bones were broken into fragments and incubated for 1 hour at 37 °C in phosphate-buffered saline (PBS) supplemented with 1 mg/ml collagenase type I (Thermo Fisher, Waltham, US). The supernatants were filtered through 100 μm pores of a cell strainer (Greiner Bio-One ...
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bioRxiv - Neuroscience 2020Quote: ... hindlimb tibialis anterior (TA) muscles from E18.5 mouse embryos were explanted and digested in 0.2% collagenase type I (Gibco-Life Technologies, catalog no. 17100-017) in DMEM for 60 min at 37°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... Wild-type I-OnuI was previously cloned between the NcoI and NotI cut sites on the pProEX-HT-a plasmid (Invitrogen and Life Technologies) and pEndo backbone49 ...
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bioRxiv - Cancer Biology 2024Quote: ... Collagen was prepared by mixing 500 uL of collagen with 62.5 uL of 10x minimum essential medium (Life Technologies) and neutralized with 0.8 M NaHCO3 to reach a neutral pH ...
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bioRxiv - Cancer Biology 2021Quote: ... were evenly coated with 75 µL of a 1 mg/mL collagen solution composed of 3 mg/mL rat tail collagen (Gibco), serum free media ...
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bioRxiv - Bioengineering 2019Quote: ... Purity of the collagen was verified via SDS-PAGE and mass spectroscopy by comparison of a commercially available rat tail collagen solution (Invitrogen).
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bioRxiv - Bioengineering 2020Quote: Total cell number of pASC on mineralized collagen and mineralized collagen-amnion scaffolds was calculated using a Hoechst 33258 (Invitrogen) DNA quantification method over the course of 28 days (day 0 ...
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bioRxiv - Cell Biology 2021Quote: ... coated with Cellmatrix Type I-C (Nitta Gelatin, Osaka, Japan) at 37 °C with 5% CO2 in FluoroBrite DMEM (A1896701, Thermo Fisher Scientific, Massachusetts, USA) supplemented with 10% FBS (FB-1365 ...
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bioRxiv - Cancer Biology 2022Quote: ... and digested at 37°C for 30 minutes with continuous agitation in a solution of Advanced DMEM/F12 containing the following enzymes: Collagenase Type I (Thermo Fisher Scientific, 450U/ml), Dispase (Corning ...
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bioRxiv - Cancer Biology 2023Quote: ... and digested at 37°C for 30 min with continuous agitation in a solution of Advanced DMEM/F12 containing the following enzymes: Collagenase Type I (Thermo Fisher Scientific, 450U/ml), Dispase (Corning ...
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bioRxiv - Neuroscience 2021Quote: ... and desoxyribonuclease I (DNAse I, 150U/μL, Gibco) for 10-15 minutes at 37°C after which the remaining fragments were mechanically homogenized by gentle pipetting ...
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bioRxiv - Cell Biology 2022Quote: ... or Collagen IV (2 mg/ml, Life Technologies) (for adaptive and NK cells ...
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bioRxiv - Pathology 2020Quote: ... collagen gel was first dissolved using collagenase (Gibco™ Collagenase ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-Collagen II (Thermo Scientific MS235B, 1:100), anti-GPCR GPR126 (ADGRG6 ...
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bioRxiv - Cancer Biology 2023Quote: ... and anti-Collagen IV (Thermo Scientific, Cat#SAB4200500) and secondary antibodies conjugated with Alexa fluor 488 ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-collagen IV (1:200, Thermo Fisher Scientific), anti-collagen VI (1:200 ...
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bioRxiv - Cell Biology 2020Quote: ... cell suspension-matrigel mixtures in the transwells were washed with PBS and incubated in a digestion cocktail of Collagenase Type I (Cat#17100017; Thermo Fisher; used 225 U/mL), Dispase II (Cat#17105041 ...
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bioRxiv - Cell Biology 2020Quote: ... whole lung was dissected from adult animals and perfused with a digestion cocktail of Collagenase Type I (Cat#17100017; Thermo Fisher; used 225 U/mL), Dispase II (Cat#17105041 ...
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bioRxiv - Immunology 2021Quote: ... Lungs were inflated with an enzymatic digestion buffer (Dubecco’s modified Eagle’s medium [DMEM], 1 mg/mL collagenase type IV [Invitrogen], and 0.2 mg/mL DNase I [Sigma]), after which they were dissected away from the trachea and heart and incubated in 5 mL enzymatic digestion buffer in a 50 mL conical tube for 30 min at 37°C under agitation at 200 rpm ...
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bioRxiv - Developmental Biology 2023Quote: ... dissected in ice-cold PBS and then digested with 4mg/ml Collagenase Type IV/ 0.2mg/ml DNase I /10% FCS/PBS under constant shaking with 700 rpm at 37°C for 12-15 min (Collagenase Type IV, Cat#: 17104-019, Gibco; DNase I, Cat#: 10104159001, Roche). Samples were filtered using a 70µm nylon filter and then washed twice with FACS buffer (0.5% FCS/2mM EDTA in PBS ...
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bioRxiv - Microbiology 2020Quote: ... After DNase I digestion with DNAse I (Invitrogen™), RNA concentration was measured using Quant-iT™ RiboGreen® RNA Assay Kit (Life Technologies) ...
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bioRxiv - Developmental Biology 2019Quote: ... HSA+ cells were plated in 0.1% collagen (Life Technologies) for E15.5 or fibronectin/gelatin for postnatal cells ...