Labshake search
Citations for Thermo Fisher :
301 - 350 of 4418 citations for PhotoCol Methacrylated Type I Collagen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: ... Cutting up the adipose tissue then digesting them fully by collagenase type I (Invitrogen, Carlsbad, CA, USA) staying at 37℃ shaking bath (50rpm/min ...
-
bioRxiv - Bioengineering 2019Quote: ... samples were covered with protease solution (collagenase type I, Gibco, 50 U/mL in PBS, 37°C). At each timepoint ...
-
bioRxiv - Biochemistry 2019Quote: ... smegmatis wild-type or Rv1626D65E over-expression strain was used for DNase I (AM2238, Thermo Fisher Scientific) treatment ...
-
bioRxiv - Developmental Biology 2021Quote: ... anterior tips of ovaries were incubated in dissociation solution (0.5% Type I Collagenase (Thermo Fisher Scientific #17018029), 1% Trypsin 1:250 (Thermo Fisher Scientific #27250018 ...
-
bioRxiv - Genetics 2022Quote: ... and the brain were minced and digested with 500 μL of 1mg/mL collagenase type I (Gibco) by incubating at 37°C ...
-
bioRxiv - Cancer Biology 2023Quote: Tumors or pancreata were minced and digested in 1.5 mg/mL type I collagenase (Thermo Fisher Scientific) in 5 mL PBS at 37°C for 20 minutes ...
-
bioRxiv - Cancer Biology 2023Quote: ... Mouse prostates were disaggregated using 1mg/ml type I collagenase and 1mg/ml Dispase (Invitrogen, Carlsbad, CA) in RPMI medium and filtered through 40 μm cell strainer ...
-
bioRxiv - Bioengineering 2024Quote: ... collected AF tissue was finely minced and digested using collagenase type I (400 U/mL, Gibco, USA) for 12 hours under constant agitation at 37°C in serum-free hg-DMEM containing Penicillin / Streptomicin and Amphotericin B (0.25 µg/mL) ...
-
bioRxiv - Microbiology 2020Quote: ... 2×104 cells were resuspended in serum-free medium and applied to the top of a membrane coated with collagen I (rat-tail) (Gibco- A1048301). Transmigration assays were carried out using a Corning Transwell Costar apparatus (6.5 mm diameter and 8 µm pore size ...
-
bioRxiv - Cell Biology 2021Quote: ... Collagen matrix was prepared on ice by adding 10x PBS (to make 1x PBS) and 1N NaOH (to make 25mM NaOH) to Collagen I (Rat tail, Thermo Fisher). Organoids were treated with ERW medium ...
-
bioRxiv - Bioengineering 2022Quote: hCMEC/D3 brain endothelial cell line was maintained in tissue culture flasks pre-coated with 0.15 mg/mL rat collagen I in a humidified incubator at 37 ºC and 5% CO2 (Isotemp, Thermo Fisher Scientific). hCMEC/D3 cells were maintained in complete growth medium comprising of endothelial cell basal medium (EBM-2 ...
-
bioRxiv - Immunology 2021Quote: ... DCs were then added to 96-well plates pre-cultured with bEnd.3 cells or embedded in bovine collagen I (1 mg/ml, Life Technologies). Live cell imaging was performed for 1 h ...
-
bioRxiv - Cell Biology 2023Quote: ... The surface of the gel was then rendered permissive to cell adhesion by coating Rat Tail collagen I (Gibco, Thermo Fisher Scientific ...
-
bioRxiv - Bioengineering 2022Quote: ... collagen II (MA512789; Thermofisher), or collagen X (14-9771-82 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Rat tail collagen (Gibco) was prepared at 1.8-2mg/mL in 0.08N acetic acid ...
-
bioRxiv - Genomics 2020Quote: Optimal establishment of primary human hepatocytes in tissue culture required a substratum of type 1 collagen (24-well plates; ThermoFisher Scientific #A11428-02) and initial plating at 200,000 cells/well in complete Williams’ E medium containing supraphysiological concentrations of insulin ...
-
bioRxiv - Developmental Biology 2020Quote: ... The remaining dermal tissues were digested for 45 min in collagenase type I (220 U/ml; Life Technologies), filtered through a 70 μm strainer and centrifuged ...
-
bioRxiv - Neuroscience 2022Quote: ... The DRGs were incubated in DRG enzyme solution (1000 U/ml DNAse I, 3.75 mg/ml dispase and 0.8 mg/ml collagenase type 1A; Gibco) for 21 min at 37°C in a 5 % CO2 incubator ...
-
bioRxiv - Neuroscience 2020Quote: ... hindlimb tibialis anterior (TA) muscles from E18.5 mouse embryos were explanted and digested in 0.2% collagenase type I (Gibco-Life Technologies ...
-
bioRxiv - Neuroscience 2024Quote: ... The cortex was isolated and digested with 0.1% type IV collagenase with 30 u/ml DNase I (Gibco) at 37 °C for 1.5 hours ...
-
bioRxiv - Bioengineering 2024Quote: ... The AT constructs were digested in 100 µL of 35 mg/mL collagenase type I (Gibco, Cat # 17100017) for 30 minutes at 37°C ...
-
bioRxiv - Immunology 2021Quote: ... The rat anti-CD68 mAb (catalog number 14-0681-82) and the rabbit polyclonal anti-mouse collagen I (catalog number PA5-95137) were from Invitrogen (Carlsbad, CA). The mouse anti-fibrillin-1 mAb (catalog number MAB2502 ...
-
bioRxiv - Genetics 2019Quote: Coverslips were incubated for one hour at 37°C in 1mL of of 1:30 solution of Collagen I Rat Protein (Thermo Fisher Scientific) to 1X PBS ...
-
bioRxiv - Bioengineering 2022Quote: ... culture dishes were coated at 4 °C overnight with collagen I at a 1:8 concentration diluted in ddH2O (Gibco, #A10483-01). The next day ...
-
bioRxiv - Bioengineering 2022Quote: ... The rat tail collagen I protein and GFOGER peptide peptide were subsequently blocked using methyl-PEG24-amine (MA-PEG24) (Thermo Fisher Scientific). Post functionalization ...
-
bioRxiv - Immunology 2023Quote: Passage 0 epithelial cells were cultured in plastic cell culture vessels without an irradiated 3T3-J2 monolayer and seeded in a Collagen I (1:100, Fisher Scientific, A1064401) pre-coated T25 flask ...
-
Plasma Activated Water as a Pre-Treatment Strategy in the Context of Biofilm-Infected Chronic WoundsbioRxiv - Microbiology 2023Quote: ... wells of 96-well flat bottom microtiter plates were pre-coated with 300 µg/mL collagen I from rat tail (Gibco, Life Technologies, USA) for 1 h at 37°C ...
-
bioRxiv - Bioengineering 2022Quote: ... The other half was stained with the type II collagen antibody (prior to fixing with 4% PFA) by incubating the primary antibody (Invitrogen, MA5-13026, 1:100) for 2 hours followed by fixing for 30 minutes and a secondary stain for 2 hours (Abcam ...
-
bioRxiv - Genomics 2022Quote: ... These pieces were then transferred to fresh tubes and incubated in 200U/ml Collagenase type I (Gibco 17100-017) and 3mM calcium chloride in PBS for three cycles of 10 minutes under mild agitation at 37°C in 1.5 ml eppendorf tubes ...
-
bioRxiv - Immunology 2022Quote: ... and MDA5-/-/RIG-I-/- double knockouts) or wild type cells were transfected with Silencer Select small interfering RNAs (Ambion) targeting SAMHD1 (sense RNA 5’-GCAGAUAAGUGAACGAGAUTT-3’ ...
-
bioRxiv - Cell Biology 2023Quote: ... Postnatal and adult mouse tissues were first digested for 20min at 37°C with 2.5 mg/mL collagenase type I (Thermofisher), 2.5 mg/mL dispase II (Thermofisher) ...
-
bioRxiv - Developmental Biology 2024Quote: ... The tunica albuginea was removed and placed into PBS with 120 U/ml Type I Collagenase (Gibco, 17100-017) on a shaker at 37°C and 220 rpm for 10 min ...
-
Plasma Activated Water as a Pre-Treatment Strategy in the Context of Biofilm-Infected Chronic WoundsbioRxiv - Microbiology 2023Quote: ... wells of 96-well flat bottom microtiter plates were pre-coated with 300 µg/mL collagen I from rat tail (Gibco, Life Technologies, USA) for 1 h at 37°C ...
-
Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... pCAFs and pNFs were cultured on dishes coated with collagen I from rat tail (12 μg/ml, Gibco, Thermo Fisher Scientific, Waltham, MA). Unless otherwise stated ...
-
Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... pCAFs and pNFs were cultured on dishes coated with collagen I from rat tail (12 μg/ml, Gibco, Thermo Fisher Scientific, Waltham, MA). Unless otherwise stated ...
-
bioRxiv - Bioengineering 2022Quote: ... collagen was acquired from Gibco. Phosphate buffer saline (PBS ...
-
bioRxiv - Bioengineering 2021Quote: ... collagen were purchased from Gibco and Phosphate buffer saline (PBS ...
-
bioRxiv - Cancer Biology 2024Quote: ... Collagen IV (Invitrogen #PA5-104508), pDDR1 (Tyr513 ...
-
bioRxiv - Developmental Biology 2023Quote: ... Collagen substrate was prepared with 400 μl of 3 mg/ml rat-tail collagen (Gibco) in 5 μl 1M HEPES (pH 7 ...
-
Optimized immunoglobulin knock-ins using Cas9 reveal peritoneal B cell lineage relationships in vivobioRxiv - Immunology 2021Quote: ... Intestinal pieces were then placed into 10 mL RPMI 20% FCS with 0.5 mg/mL Collagenase Type I (Gibco #17100017) and 0.1 mg/mL DNase I (Sigma #DN25 ...
-
bioRxiv - Developmental Biology 2021Quote: ... 354235], 50 μL of DNase [5 mg/mL, GoldBio, D-301], 100 μL of Collagenase Type I [480U/mL, Gibco, 17100-017] in 9 mL of PBS [Gibco ...
-
bioRxiv - Cell Biology 2022Quote: Primary mouse pre-osteoblasts were isolated from the calvaria of 3-day-old C57BL/6J mice via sequential digestion with type I collagenase (Gibco) and dispase (Roche) ...
-
bioRxiv - Cell Biology 2022Quote: ... mandibular alveolar bone tissue was cut into small pieces (<1 mm3) and digested with 3 mg/mL collagenase type I (Gibco) and 4 g/mL dispase II (Sigma ...
-
bioRxiv - Developmental Biology 2022Quote: ... and 100 μL of Collagenase Type I (stock: 48,000 U/mL; final concentration of 480 U/mL, Gibco, 17100-017)] ...
-
bioRxiv - Immunology 2023Quote: ... excised 4cm2 skin sections were diced into small pieces with a scalpel blade and enzymatically digested with Collagenase type I (500 U/ml; Gibco) and DNAse I (1 mg/ml ...
-
bioRxiv - Immunology 2023Quote: ... then incubated in wash buffer (Advanced DMEM, 10% FBS, 2 mM L-glutamate, penicillin-streptomycin) with 2 mg/mL collagenase type I (Invitrogen) at 37°C for 5-15 mins with vigorous mixing as previously described.57 Crypts were plated in Matrigel with Human Organoid Growth Media (StemCell) ...
-
bioRxiv - Developmental Biology 2023Quote: Lung tissue was minced with a razor blade and resuspended in a digestion buffer containing of 480 U/ml Collagenase Type I (Gibco), 50 U/ml Dispase (Corning) ...
-
bioRxiv - Genetics 2021Quote: ... collagen-coated well (Gibco, A11428-02) and passaged 1:3 while subconfluent until reaching 85% confluence on a 10cm diameter dish ...
-
bioRxiv - Bioengineering 2019Quote: ... Collagen 1 (ThermoFisher; A1048301; 60 μL) at 4 mg/mL pH neutralized ...
-
bioRxiv - Systems Biology 2020Quote: ... Collagen (Thermo Fisher Scientific, Catalogue #A1048301), sterile 10X phosphate buffered saline (Thermo Fisher Scientific ...