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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Peripheral Blood Mononuclear Cell since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... peripheral blood mononuclear cells were re-suspended in RPMI (Life Technologies) and monocytes purified by adherence for 1 h at 37 °C ...
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bioRxiv - Immunology 2020Quote: ... peripheral blood mononuclear cells were re-suspended in RPMI 1640 (Life Technologies), and monocytes were purified by adherence for 2?hours at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from the whole blood by Ficoll-Paque (GE Healthcare, ThermoFisher) density gradient centrifugation ...
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bioRxiv - Microbiology 2021Quote: ... Plasma and peripheral blood mononuclear cells were collected with heparinized Natelson tubes (Fisher Scientific) into 8mM EDTA in PBS ...
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bioRxiv - Immunology 2019Quote: Cryopreserved peripheral blood mononuclear cells (PBMC) were thawed in RPMI (Gibco, Life Technologies, USA) at 37°C ...
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bioRxiv - Immunology 2019Quote: Cryopreserved peripheral blood mononuclear cells (PBMC) were thawed in RPMI (Gibco, Life Technologies, USA) at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... peripheral blood mononuclear cells (PBMCs) were re-suspended in RPMI for osteoclasts (Life Technologies) and MEM-REGA 3 (1X ...
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bioRxiv - Immunology 2023Quote: Peripheral blood mononuclear cells (PBMC) were isolated using ACK Lysing Buffer (Thermo Fisher, USA). Fresh blood was diluted 1:10 in ACK lysing buffer and rotated at room temperature for 30 minutes until lysis was complete ...
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bioRxiv - Immunology 2019Quote: Peripheral blood mononuclear cells (PBMC) were isolated from blood diluted 1:1 with basic medium (RPMI 1640 (Gibco) with Penicillin/Streptomycin (Gibco) ...
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bioRxiv - Microbiology 2023Quote: Peripheral Blood Mononuclear Cells (PBMC) from normal adult donor blood were isolated using Ficoll-Paque PLUS (Fisher Scientific). After purification ...
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bioRxiv - Immunology 2020Quote: ... whole cell lysates of peripheral blood mononuclear cells were lysed on ice with LDS NuPAGE (Invitrogen) at a concentration of 105 cells per 15ul of LDS ...
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bioRxiv - Immunology 2021Quote: ... peripheral blood mononuclear cells (PBMC) and monocytes were cultured in RPMI 1640 (Invitrogen, Gaithersburg, MD) (R10 ...
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bioRxiv - Immunology 2019Quote: ... cryopreserved peripheral blood mononuclear cells (PBMCs) were thawed and stimulated in R20 media (RPMI media (Invitrogen) with 20% Fetal Bovine Serum (Gemini Bioproducts) ...
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bioRxiv - Neuroscience 2021Quote: ... peripheral blood mononuclear cells were growth for 1 week in DMEM media (Cat. 32430-027, Gibco) supplemented with 10% FBS (Fetal Bovine Serum Ultra-Low Endotoxin ...
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bioRxiv - Immunology 2020Quote: Frozen human peripheral blood mononuclear cells (PBMCs) from blood donors were thawed and diluted into cold RPMI 1640 medium (Gibco) supplemented with 10% (v/v ...
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bioRxiv - Immunology 2021Quote: ... Fresh human peripheral blood mononuclear cells were used as effector cells after red blood cell lysis with ACK lysing buffer (ThermoFisher Scientific, Waltham, MA). After 1 h incubation at 37°C ...
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bioRxiv - Neuroscience 2019Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated using Ficoll and growth in StemPro-34 SFM Medium (ThermoFisher), supplemented with L-Glutamine (2 mM ...
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bioRxiv - Immunology 2021Quote: ... Peripheral blood mononuclear cells (PBMCs) were washed twice with Hanks Balanced Solution without Ca+ or Mg+ (Gibco) at 200-400xg for 10 min ...
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bioRxiv - Genomics 2024Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from the interphase and washed twice using MACs buffer (Gibco™ Dulbecco’s phosphate-buffered saline (DPBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Fresh peripheral blood mononuclear cells were isolated within 48 hours of blood collection utilizing a Ficoll (Thermo Fisher Scientific, #45-001-750) gradient according to manufacturer’s recommendations ...
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A Novel Mice Model of Catecholaminergic Polymorphic Ventricular Tachycardia Generated by CRISPR/Cas9bioRxiv - Molecular Biology 2021Quote: ... Peripheral blood mononuclear cells were reprogrammed to pluripotency using the CytoTune Sendai reprogramming kit (Thermo Fisher, MA, USA). These colonies were then stained for the pluripotency markers SOX2 ...
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bioRxiv - Immunology 2022Quote: ... Austria) and peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation (Ficoll-Paque, GE Healthcare, ThermoFisher). The PBMCs were washed and cryopreserved in freezing medium (8% DMSO in FBS ...
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bioRxiv - Microbiology 2021Quote: Peripheral blood mononuclear cells (PBMCs) were isolated from healthy human donors by performing a Ficoll-Paque gradient (Thermo Fisher) on whole blood ...
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bioRxiv - Cell Biology 2022Quote: ... This line was reprogrammed from peripheral blood mononuclear cells of a healthy donor with the Cytotune reprogramming kit (ThermoFisher). The process of reprogramming and quality control profiling for this iPSC was outlined in a previous study (Chen et al. ...
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bioRxiv - Molecular Biology 2023Quote: CD19+ B cells were isolated from PBMCs (peripheral blood mononuclear cells) using Dynabeads CD19+ pan B (11143D, Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2022Quote: ... Germline DNA were extracted from frozen peripheral blood mononuclear cells (PBMCs) using PureLink Genomic DNA Mini Kit (Thermo Scientific, #K182001) following manufacturer instructions ...
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bioRxiv - Neuroscience 2023Quote: ... AIW002-2 was reprogrammed from peripheral blood mononuclear cells of a healthy donor with the Cytotune reprogramming kit (Thermo Fisher). 3450 was also generated from peripheral blood mononuclear cells ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary human peripheral mononuclear blood cells (PBMCs) were cultured at 37 °C in 5% CO2 in RPMI 1640 (ThermoFisher, 61870143) supplemented with 10% FBS ...
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bioRxiv - Genomics 2024Quote: ... The peripheral blood mononuclear cell (PBMC) layer was carefully collected and subsequently resuspended in 500 µL of ACK lysis buffer (Gibco), followed by a 3-minute incubation at room temperature ...
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bioRxiv - Immunology 2023Quote: ASCs (FVS780-CD20-CD3-CD14-CD16-CD19+CD27hiCD38hi) were sort-purified from human peripheral blood mononuclear cells into culture media (10% FCS, 2 mM L-glutamine (Gibco), 100 units/mL Penicillin and 100 ng/mL Streptomycin (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2019Quote: iPSCs were reprogrammed from peripheral blood mononuclear cells using the Sendai virus approach (CytoTune-iPS 2.0 Sendai Reprogramming Kit - Thermo Fisher Scientific) according to the manufacturer instruction ...
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bioRxiv - Immunology 2020Quote: Human peripheral blood mononuclear cells (PBMCs) from SARS-CoV-2 convalescent donors were stained with Live/Dead Fixable Aqua (Invitrogen; Thermo Scientific) in 100 μL final volume diluted 1:500 at room temperature (RT) ...
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bioRxiv - Genomics 2022Quote: Peripheral blood mononuclear cells (PBMCs) were reprogrammed to hiPSCs using the CytoTune™-iPS 2.0 Sendai Reprogramming Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions with modifications as previously described (Feyen et al ...
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bioRxiv - Immunology 2020Quote: Human peripheral blood mononuclear cells (PBMCs) from SARS-CoV-2 convalescent donors were stained with Live/Dead Fixable Aqua (Invitrogen; Thermo Scientific) in 100 μL final volume diluted 1:500 at room temperature (RT) ...
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bioRxiv - Immunology 2022Quote: ... Cryopreserved peripheral blood mononuclear cells from healthy patient were thawed and resuspended at 1 million/ml in RPMI medium (Gibco, Life Technologies) supplemented with 10% heat-inactivated fetal bovine serum (FBS) ...
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bioRxiv - Neuroscience 2023Quote: ... The other iPSC lines were reprogrammed from peripheral blood mononuclear cells (PBMCs) using CytoTune iPS Sendai reprogramming kit (Thermo Fisher Scientific) according to the manufacture’s protocol with the adequate quality control criteria for iPSC validation.
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bioRxiv - Immunology 2023Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation using Cytiva Ficoll-Paque PLUS (45-001-749, Fisher Scientific).
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bioRxiv - Neuroscience 2024Quote: ... Human peripheral blood mononuclear cells (ATCC #PCS-800-011) were thawed as recommended and resuspended in RPMI 1640 medium (Gibco #11875093) with 1X GlutaMAX (Gibco #35050061) ...
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bioRxiv - Immunology 2020Quote: Human peripheral blood mononuclear cells (PBMCs) from SARS-CoV-2 convalescent donors were stained with Live/Dead Fixable Aqua (Invitrogen; Thermo Scientific) in 100 μL final volume diluted 1:500 at room temperature (RT) ...
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bioRxiv - Immunology 2020Quote: Human peripheral blood mononuclear cells (PBMCs) from SARS-CoV-2 convalescent donors were stained with Live/Dead Fixable Aqua (Invitrogen; Thermo Scientific) in 100 μL final volume diluted 1:500 at room temperature (RT) ...
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bioRxiv - Immunology 2022Quote: ... Cryopreserved peripheral blood mononuclear cells from healthy patient were thawed and resuspended at 1 million/ml in RPMI medium (Gibco, Life Technologies) supplemented with 10% heat-inactivated fetal bovine serum (FBS) ...
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bioRxiv - Immunology 2023Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from samples using density gradient centrifugation in Ficoll-Paque Plus Media (Fisher Scientific, Waltham, Massachusetts).
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bioRxiv - Immunology 2019Quote: ... from RNA extracted from peripheral blood mononuclear cells (PBMCs) utilizing the following primers: forward 5’-GAGAATTCACCATGACTATGGAGACCCAAATG-3’ and reverse 5’-CGTACGCCCCATTGGTGAAGAGCTGCC-3’ (Thermo Fisher Scientific, Waltham, MA, USA). PCR product was fused by restriction enzyme-compatible ends with the CD8α-CD28-CD3ζ domain contained in the pcDNA3.1/V5-His TOPO TA (Invitrogen ...
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bioRxiv - Immunology 2019Quote: ... from RNA extracted from freshly isolated peripheral blood mononuclear cells (PBMCs) utilizing the following primers: forward 5’-GAGAATTCACCATGACTATGGAGACCCAAATG-3’ and reverse 5’-CGTACGCCCCATTGGTGAAGAGCTGCC-3’ (Thermo Fisher Scientific, Waltham, MA, USA). The PCR product was fused in tandem by restriction enzyme-compatible ends with the CD8α transmembrane domain and the CD28 and CD3ζ intracellular regions already available in the lab (CD32131R-CR) ...
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bioRxiv - Cell Biology 2022Quote: ... 6 ×105 peripheral blood cells were resuspended in 100 μl RPMI 1640 (Gibco) plus 10% FBS and added to the upper insert to continue for two- hour incubation at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... and peripheral red blood cells were lysed using ACK lysis buffer (Thermo Fisher Scientific). Organ cellularity was determined by an automated CASY cell counter (OMNI Life Science 5651736).
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bioRxiv - Microbiology 2022Quote: ... Peripheral blood mononuclear cells (PBMCs) were recovered from EDTA-blood samples after erythrocyte removal by treatment with ACK lysing buffer (Gibco) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from the patient’s blood and cultured in RPMI 1640 with 10% FBS (Gibco, 6140-079) for 24 hours to allow monocyte cells to attach ...
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bioRxiv - Cancer Biology 2021Quote: ... NSB-75 and peripheral blood B cells (PBBC) were cultured in RPMI+10% FBS (Gibco), supplemented with 2mM L-glutamine (Sigma) ...
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bioRxiv - Immunology 2021Quote: Human Peripheral Blood T (hPBT) cells have been cultured in RPMI 1640 medium (Thermo Fisher 21875) supplemented with 10% Fetal Bovine Serum (Thermo Fisher 10270) ...