Labshake search
Citations for Thermo Fisher :
401 - 450 of 10000+ citations for Peripheral Blood Mononuclear Cell since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... we isolated human neutrophils from whole blood and stained cells with Calcein AM (C1430, Thermofisher Scientific) at 4 °C for 1h in the dark ...
-
bioRxiv - Immunology 2023Quote: ... Red blood cells were lysed using an Ammonium-Chloride-Potassium (ACK) lysis buffer (Thermo Fisher Scientific). Fifty thousand Trp1 CD4+ T cells in PBS were adoptively transferred via the tail vein into B16F10 melanoma-bearing mice ...
-
bioRxiv - Immunology 2023Quote: ... Red blood cells were lysed by a 1 min incubation in ACK lysis buffer (Thermofisher Scientific). Cells were centrifuged at 1400 rpm at 4°C for 10 min and pellets washed in PBS/2% FCS ...
-
bioRxiv - Immunology 2024Quote: ... Residual red blood cells were lysed by 5 mL of ACK lysing buffer (Gibco, Waltham, MA) for 10 mins ...
-
bioRxiv - Physiology 2024Quote: ... The SVF pellet was resuspended in 1 mL of Red Blood Cell ACK Lysis Buffer (Gibco) and incubated at room temperature for 3-5 min to lyse red blood cells ...
-
bioRxiv - Immunology 2024Quote: ... using standard procedures including red blood cell lysis with ACK lysis buffer (Lonza by Thermo Fisher). Before cell staining ...
-
bioRxiv - Immunology 2021Quote: ... and Columbia Blood Agar (ThermoFisher, #R01217) at 37C for 48-72 hours ...
-
bioRxiv - Immunology 2019Quote: Blood was collected in EDTA (Thermofisher) coated syringes by cardiac puncture under terminal anaesthesia (sodium-pentobarbital i.p.) ...
-
bioRxiv - Microbiology 2019Quote: ... and Phusion Blood Direct (Thermo Fisher). Hemo KlenTaq reactions were set up as 25 µL reactions containing 5 μL Hemo KlenTaq reaction buffer ...
-
bioRxiv - Microbiology 2021Quote: ... and 5% sheep blood (Life Technologies) and then covered with a cover glass ...
-
bioRxiv - Microbiology 2020Quote: ... 5% sheep blood (Thermo Fisher Scientific) and/or 5 μg/ml hemin (Sigma-Aldrich) ...
-
bioRxiv - Immunology 2023Quote: ... Blood was diluted with PBS (Gibco) and overlaid on lymphocyte separation medium (MP Biomedical) ...
-
bioRxiv - Immunology 2019Quote: ... Red blood cells (RBC) were removed by incubating the cell suspensions in 5 mL 1X RBC Lysis Buffer (eBioscience, Thermo Fisher) for 5 minutes at room temperature ...
-
bioRxiv - Cell Biology 2021Quote: ... Single cell-suspensions of lymphocytes were achieved by sterile dissociation of whole spleens with syringe plunger through cell strainer and by lysing red blood cells with the ACK (Ammonium-Chloride-Potassium) lysing buffer (Life Technologies). Isolated splenocytes were pooled together and placed in 24-well plates at 106 cells per ml concentration in 1.5 ml and activated with anti-CD3 mAb (145-2C11 clone ...
-
bioRxiv - Immunology 2023Quote: ... Single-cell suspensions of spleens were prepared using 70-μm cell strainers and red blood cells lysed by incubation in Ammonium-Chloride-Potassium (ACK) Lysing Buffer (Life Technologies). Single-cell suspensions of lungs were prepared by digestion with 0.25mg/ml Collagenase IV and 15 μg/ml DNase for 1 hour at 37°C ...
-
bioRxiv - Immunology 2023Quote: Single-cell suspensions of spleen tissues were prepared and depleted of red blood cells by ACK lysing buffer (#A1049201, ThermoFisher Scientific). For flow cytometric analyses ...
-
bioRxiv - Immunology 2023Quote: ... spleens of naïve CD45.1 OT-II mice were harvested and homogenized by filtering through a 70-μm cell strainer and red-blood cells were lysed with ACK buffer (Thermo Scientific). CD4+ T cells were isolated by negative selection using a cocktail of biotinylated antibodies targeting Ter119 ...
-
bioRxiv - Immunology 2024Quote: ... single-cell suspensions of spleen tissues were prepared and depleted of red blood cells using ACK lysing buffer (#A1049201, Fisher Scientific). Splenocytes were incubated with antibodies against a panel of surface markers at 4°C for 30 minutes ...
-
bioRxiv - Immunology 2023Quote: ... contaminating red blood cells were lysed by resuspending cells for 5 minutes in 5 mL of ice-cold ACK Lysis buffer (Gibco, A1049201). The cells were then pelleted at 500 x g for 5 minutes ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... immature peripheral neurons were frozen in 90% fetal bovine serum (FBS) (Thermo Fisher Scientific, Waltham, MA, USA) and 10% dimethyl sulfoxide (DMSO) ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... immature peripheral neurons were frozen in 90% fetal bovine serum (FBS) (Thermo Fisher Scientific, Waltham, MA, USA) and 10% dimethyl sulfoxide (DMSO ...
-
bioRxiv - Neuroscience 2022Quote: ... the total RNA of the aforementioned three pairs of peripheral nerves was prepared using TRIzol Reagent (Invitrogen). The RNA integrity number was evaluated by Agilent 2200 TapeStation (Agilent Technologies ...
-
bioRxiv - Immunology 2019Quote: ... approximately 3ml of whole blood was collected into a Tempus™ Blood RNA Tube (Life Technologies #4342792) and frozen at −20°C for a minimum of 30 days ...
-
bioRxiv - Cancer Biology 2020Quote: Blood vessel patency analysis was performed injecting 10 ul of quantum dots blood tracers (655nm Life Technologies) through the tail vein of the animals (Supp Videos 1 and 2) ...
-
bioRxiv - Neuroscience 2022Quote: ... blood was collected by either cardiac puncture using K3EDTA blood collection tubes (Fisher Scientific, 22-040-035) immediately before perfusion ...
-
bioRxiv - Molecular Biology 2022Quote: ... Whole blood RNA was extracted using the GeneJET Whole Blood RNA Purification Kit (Thermo Fisher, Waltham, MA), following the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2023Quote: ... Four to five drops of blood were collected in heparinized capillary blood collection tubes (Thermo Fisher Scientific) by tail vein puncture ...
-
bioRxiv - Immunology 2019Quote: ... MLN and whole blood cells were stained for live/dead with blue reactive dye (catalog#L23105, Invitrogen) and cell surface markers were 2 labeled with the following antibody panels ...
-
bioRxiv - Immunology 2019Quote: Tumor cell suspension and whole blood were stained with LIVE/DEAD Fixable Aqua (Life Technologies, Courtaboeuf, France) for 10 min at RT ...
-
bioRxiv - Microbiology 2019Quote: ... For samples from ILRI and CIRDES: Red blood cells were lysed with ACK lysing buffer (Gibco, UK) and discarded by centrifugation ...
-
bioRxiv - Cancer Biology 2020Quote: ... Pellet was washed twice in PBS and resuspended in 10ml 1X red blood cell lysis buffer (Thermofisher) and incubated for 5 minutes at RT ...
-
bioRxiv - Cancer Biology 2020Quote: ... Pellet was washed twice in PBS and resuspended in 10ml 1X red blood cell lysis buffer (Thermofisher) and incubated for 5 minutes at RT ...
-
bioRxiv - Microbiology 2020Quote: ... Red blood cell lysis was performed using 2 ml/spleen of 1X ACK lysing buffer (Life Technologies). Cells were then washed in RPMI 1640 containing 10% heat-inactivated fetal bovine serum (Atlanta Biotech ...
-
bioRxiv - Cancer Biology 2019Quote: ... Pellet was washed twice in PBS and resuspended in 10ml 1X red blood cell lysis buffer (Invitrogen) and incubated for 5 minutes at RT ...
-
bioRxiv - Pathology 2019Quote: Red blood cells (RBCs) with and without parasites were fluorescently labelled with CMTPX (Thermofisher, Waltham, MA, USA). All labelling and incubation steps took place at 37°C under constant agitation ...
-
bioRxiv - Cancer Biology 2023Quote: ... depletion of red blood cells was carried out with RBC lysis buffer (00-4333-57, Thermo Fisher), stromal cell enrichment was performed using anti-Cd45 (clone 30-F11 ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cell suspensions were then filtered using a 40μm cell strainer (Falcon, Cat. No. 352340) and red blood cells (RBC) were removed by RBC lysis buffer (Invitrogen, Cat. No. 1966634). Dissociated cells were washed twice with PBS 0.1% BSA buffer with cold centrifuging at 500rpm and 5’ ...
-
bioRxiv - Cancer Biology 2023Quote: ... Undigested tissue particles were filtered through a 70 μm cell strainer and the single cell suspension was subjected to Red Blood Cell (RBC) lysis using ACK (Ammonium, Chloride, Potassium) lysis buffer (Gibco, NY, USA) for 3-5 minutes on ice ...
-
bioRxiv - Cancer Biology 2023Quote: ... the pelleted cells were suspended in 3 ml of 1X Red Blood Cell (RBC) lysis buffer (ThermoFisher, Cat. No. 00-4300-54) and incubated on ice for 10 minutes ...
-
bioRxiv - Cancer Biology 2023Quote: ... Spleens were homogenized by filtering through a 40 µm cell strainer followed by red blood cell lysis using ACK Lysing Buffer (A1049201, Thermo Fisher Scientific). Cells were resuspended in PBS with 0.5% BSA ...
-
bioRxiv - Neuroscience 2020Quote: Frozen brain samples and peripheral organs were homogenized in T-PER™ Tissue Protein Extraction Reagent (Thermo Fisher), N13 cells were lysed in RIPA buffer (Thermo Scientific) ...
-
bioRxiv - Immunology 2021Quote: ... Bovine whole blood was collected and stored in Tempus TM blood RNA tubes (Applied Biosystems, Foster City, CA). Collections occurred at four-time points ...
-
bioRxiv - Bioengineering 2023Quote: Mouse blood was collected from the retro-orbital sinus into Microvette blood collection tubes with EDTA (Fisher Scientific). Red blood cells were depleted by ACK lysis (Quality Biological Inc.) ...
-
bioRxiv - Immunology 2023Quote: ... 10% H2/N2 (BOC) for 3 days on blood agar plates containing 7% laked horse blood (Thermo Scientific) and the Campylobacter selective supplement “Skirrow” containing the antibiotics trimethoprim ...
-
bioRxiv - Genomics 2019Quote: ... and Tempus Blood RNA Tubes (Applied Biosystems) for RNA isolation ...
-
bioRxiv - Immunology 2021Quote: Blood was collected in 2mM EDTA (ThermoFisher) coated syringes by cardiac puncture under terminal anaesthesia (sodium pentobarbital ...
-
bioRxiv - Immunology 2022Quote: ... Remaining blood was diluted with RPMI (Gibco), layered over an appropriate volume of Ficoll (Cytiva ...
-
bioRxiv - Genetics 2023Quote: ... using the RiboPure™-Blood Kit (Ambion). RNA amplification was performed using the SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing (TaKaRa ...
-
bioRxiv - Microbiology 2024Quote: ... with 5% Sheep’s Blood (Fisher Scientific, 50863755), and the remaining bacteria were then pelleted ...
-
bioRxiv - Molecular Biology 2019Quote: ... parasites were cultured in human red blood cells (RBC) in RPMI-1640 culture medium (Thermo Fisher # 53400-025) supplemented with 10% v/v Albumax I (Thermo Fisher # 11020039) ...