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Citations for Thermo Fisher :
401 - 450 of 6074 citations for Peptide T since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Isolated CD8+ T cells were cultured for 72 hours in T cell culture media: RPMI (GIBCO, Cat#11875-093) supplemented with 10% of heat-inactivated FBS ...
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bioRxiv - Immunology 2023Quote: ... CFSE-stained T cells were resuspended at 1e6/ml and DynabeadsTM human T-Activator CD3/CD28 beads (Gibco, #11131D) were added at a ratio of 1:10 beads:cells ...
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bioRxiv - Immunology 2024Quote: ... and naïve CD8 T cells from OT-I donors were isolated using the Dynabeads Untouched Mouse T Cells kit (Invitrogen), both according to manufactures protocol ...
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bioRxiv - Developmental Biology 2021Quote: Phospho-peptides were fractionated using alkaline reversed phase chromatography (Pierce™ High pH Reversed Phase Peptide Fractionation Kit, Thermo Scientific #84868) into 12 fractions using increasing concentrations of acetonitrile from 10%-80% in basic binding buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... All ten vials of samples were combined and approximately 100 μg peptide digests from this mix were fractionated using Pierce High pH Reversed-Phase Peptide Fractionation Kit (ThermoFisher Scientific) according to instructions from manufacturer ...
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bioRxiv - Biochemistry 2020Quote: ... samples were briefly centrifuged at 13,000 g and peptide-containing supernatant was used for further analysis Peptides were fractionated into eight fractions using High-pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher). Peptide fractions were dried in SpeedVac (Eppendorf ...
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bioRxiv - Bioengineering 2021Quote: ... then incubated with 50 µL of 1 µM biotinylated peptide in peptide binding buffer (1% sheared salmon sperm DNA (Invitrogen, AM9680), 10 mM HEPES ...
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bioRxiv - Cancer Biology 2021Quote: ... and remaining ~1.1 mg of multiplexed peptides were used for phospho-peptide enrichment by High-Select Fe-NTA Phosphopeptide enrichment kit (Thermo Fisher) after manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... The molecular mass of purified peptide was determined by 18% SDS-PAGE and the peptide concentration was obtained by Bicinchoninic protein assay kit (Thermo Scientific).
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bioRxiv - Cell Biology 2022Quote: ... TMT-labeled peptides were pre-fractionated with the basic pH reversed-phase C18 peptide fractionation kit following manufacturer’s manual (Thermo Fisher, 84868). Typically ...
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bioRxiv - Immunology 2021Quote: Sorted HSC were also cultured with blocking CD150 peptide (FCKQLKLYEQVSPPE, Auspep, 100 μg/ml) or control peptide (DLSKGSYPDHLEDGY, Auspep,100 μg/ml) (Thermo Scientific).
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bioRxiv - Biochemistry 2019Quote: ... peptide concentration and primary amine reactivity were quantified using the Pierce quantitative fluorometric peptide assay (Thermo Fisher Scientific; cat. no. 23290) and Pierce quantitative colorimetric peptide assay (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... were raised in rabbits by immunization with keyhole limpet hemocyanin-conjugated peptides and affinity-purified by columns conjugated with the peptides (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: The phosphopeptide enrichment step flow through and washes were dried down and approximately 120 µg peptides were fractionated using the TMT fractionation protocol of Pierce high pH basic reversed-phase peptide fractionation kit (Thermo Fisher Scientific™ Cat no 84858 ...
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bioRxiv - Biochemistry 2023Quote: The RC9 peptides with the sequence GGKGMGFGL was prepared using solid-state peptide synthesis with Fluorenylmethyloxycarbonyl (FMOC)-based chemistry by Life Technologies, Inc and purified by reversed-phase high pressure liquid chromatography (HPLC ...
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bioRxiv - Cell Biology 2023Quote: ... 50 mM Tris pH 7.5) followed by elution in 100 µl TBS + 150 ng/µL competitor peptide (Pierce 3x DYKDDDDK Peptide, Thermo Fisher Scientific) at 37 °C for 30 min.
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bioRxiv - Molecular Biology 2023Quote: ... 300 µL (90 µg) of reconstituted mixture of labeled peptides was fractionated using Pierce High pH Reversed-Phase Peptide Fractionation kit (84868, Thermo Fisher) according to the manufacturer’s protocol for TMT-labeled peptides ...
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bioRxiv - Cell Biology 2023Quote: ... Dried peptides were resuspended in DI water and then quantified using Pierce Quantitative Colorimetric Peptide Assay (Thermo Fisher Scientific, Rockford, USA).
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bioRxiv - Plant Biology 2024Quote: ... Labeled peptides were separated into 6 fractions with Pierce™ High pH Reversed-Phase Peptide Fractionation Kit (Thermofisher, Cat. no. 84868) and dried in a Speed Vac at 45 °C for 3.5 hours ...
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bioRxiv - Cell Biology 2024Quote: ... The deuterium exchange levels were determined by subtracting the centroid mass of undeuterated peptide from the centroid mass of deuterated peptide using HDExaminer (Version PD1.4, Thermo-Fisher Scientific, USA).
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bioRxiv - Synthetic Biology 2021Quote: ... The T cells were isolated using the EasySep human T cell isolation kit (Stemcell) and activated with human T-activator CD3/CD28 Dynabeads (Gibco) at a bead:cell ratio of 1:1 ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were then washed 4X in PBS with .5% Triton X-100 (PBS-T) and then placed in blocking solution (PBS-T with 5% normal goat serum (NGS; Invitrogen)) for 90 minutes at room temperature ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were then enriched via negative selection using Dynabeads Untouched Mouse CD4 T cells Kit (Thermo Fisher Scientific) according to manufacturer protocol.
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bioRxiv - Immunology 2022Quote: Total or individual CD8+ or CD4+ T cells were purified from PBMC suspensions from our PLWH and HIV-1 negative control study cohorts by negative immunomagnetic selection (purity >90%) using the Untouched Human T cell and CD4 T cell Dynabeads Kits (Invitrogen) and the MojoSort Human CD8 T cell (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... mice which were maintained on tamoxifen diet for 4-5 wks and from CD45.1 mice (as described above) which were subjected to T cell depletion using Dynabeads FlowComp Mouse Pan T (CD90.2) Kit (ThermoFisher Scientific). BM cells from WT or FL mice were mixed with the BM of the competitor donor at 1:1 ratio and then transplanted by intravenous injection into lethally irradiated CD45.1 mice (900 rad) ...
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bioRxiv - Biophysics 2019Quote: Jurkat T cells (Human T cell leukemia cell line– ATCC TIB-152) were grown in RPMI-1640 with Glutamax (GIBCO), supplemented with 10 % foetal calf serum and ...
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bioRxiv - Bioengineering 2020Quote: ... All T cells were expanded in complete T-cell medium and fed interleukin (IL)-2 (50 U/mL; Life Technologies) and IL-15 (1 ng/mL ...
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bioRxiv - Immunology 2021Quote: ... Naïve CD8+ T cells were activated for two days with Human T-Activator CD3/CD28 Dynabeads (ThermoFisher Scientific, Waltham, MA). After two days ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells and NK cells were isolated from cryopreserved PBMCs using commercial kits (MagniSort Human CD4+ T Cell Enrichment; Affymetrix, and MagniSort™ Human NK cell Enrichment ...
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bioRxiv - Neuroscience 2020Quote: ... with 1% PBS-T (1% Triton X-100 in 1x PBS) and blocked in 1% PBS-T containing 5% normal goat serum (ThermoFisher) for one hour ...
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bioRxiv - Microbiology 2021Quote: ... CD4+ T cells were washed with PBS and resuspended in Buffer T of Neon Transfection System 10 mL kit (ThermoFisher). Pre-assembled Cas9-gRNA protein-RNA complexes were electroporated into cells using Neon equipment (ThermoFisher) ...
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bioRxiv - Immunology 2020Quote: Peripheral T cells were isolated from mouse spleen by a CD4+ T-cell negative (Stem Cell) or positive selection kit (Invitrogen). Enriched CD4+ T cells were further sorted for either YFP+ (Foxp3+ ...
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bioRxiv - Bioengineering 2021Quote: T cells were cultured in OpTimizer CTS T Cell Expansion SFM containing 5% CTS Immune Cell SR (ThermoFisher, Waltham, MA), L-Glutamine ...
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bioRxiv - Immunology 2019Quote: ... human PBMC derived T cells were purified by Human T cell isolation kit from Invitrogen (Invitrogen Dynal AS, Oslo, Norway) according to manufacturer’s instruction ...
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bioRxiv - Cell Biology 2019Quote: ... Stable tetracycline inducible Flp-In T-REx HEK293 cell lines were generated using the T-Rex system (Thermo Fisher Scientific) as previously described (Kang et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... During this time a mixture containing 50 μl of the 3′ and 50 μl of the poly-T stock solution was freshly prepared according to poly-T manufacturer’s protocol to obtain dried ssDNA coated beads (Ambion, 2012). After T1-GHLDP separation the supernatant was transferred to the prepared ssDNA coated beads and incubated for 15 minutes at room temperature at 230 rpm ...
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bioRxiv - Microbiology 2019Quote: ... Activated CD4+ T cells were obtained by stimulating freshly isolated CD4+ T cells at 1×106/ml with T cell activator CD3/CD28 Dynabeads (ThermoFisher), at a bead-cell-ratio of 1 ...
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bioRxiv - Bioengineering 2021Quote: ... isolated CD8 T cells were cultured in T cell media [complete RPMI media supplemented with 1X nonessential amino acids (Gibco) + 1 × 10−3 M sodium pyruvate (Gibco ...
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bioRxiv - Immunology 2021Quote: ... T cells were activated for 24 hours with Dynabeads® Mouse T-Activator CD3/CD28 (Life Technologies, Grand Island, NY) in the presence of human IL-2 (30 U/ml ...
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bioRxiv - Immunology 2020Quote: ... T cells are enumerated using Trypan Blue exclusion and co-incubated with CD3/CD28 Human T cell Activating Dynabeads (ThermoFisher) at a 3:1 bead:T cell ratio in a total volume of 1 ml TCM supplemented with 50 units/ml recombinant IL-2 in a 48-well plate ...
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bioRxiv - Genomics 2021Quote: ... we stimulated these CD4+ T cells with CD3/CD28 crosslinking for 45 hours (Gibco, Dynabeads Human T-Activator CD3/CD28). We then performed ChIP-seq (2 million stimulated CD4+ T cells) ...
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bioRxiv - Cancer Biology 2021Quote: ... and enriched for CD8+ T cells with the MagniSort™ Human CD8+ T cell Enrichment Kit (#8804-6812-74, Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... then co-cultured at the indicated ratios and activated with Dynabeads Human T-Expander (1 bead:16 CD3+ T cells; Gibco) for 4 days ...
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bioRxiv - Synthetic Biology 2022Quote: ... cryopreserved PBMCs or T cells were thawed on day 0 and cultured with Human T-Activator αCD3/CD28 Dynabeads (Gibco) at 3:1 bead:cell ratio in AIM-V media (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... PBLs as well as human Jurkat T lymphocytes (human T-cell leukemia cell line) (ATCC-CRL-2899TM) were cultured in RPMI 1640 plus Glutamax (GIBCO) supplemented with 10% fetal bovin serum (FBS ...
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bioRxiv - Cancer Biology 2022Quote: ... the collected organs were homogenized and lysed using T-PER (0.5 g organ mL−1 T-PER, Thermo Fisher Scientific) supplemented with 1× Halt protease inhibitor (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... PBMCs were isolated by density gradient using Lymphoprep (Axis-Shield) and CD4+ T cells purified by negative selection using the Dynabeads Untouched Human CD4+ T Cell Isolation kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: Jurkat T cells (Clone E6-1, ATCC) or Flot1-Halo transduced Jurkat T cells were grown in complete RPMI (Gibco) media containing 2 mM L-glutamine ...
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bioRxiv - Bioengineering 2023Quote: ... The supernatant was subsequently aspirated off of the wells and 0.5×106 T cells were added in 1mL of T cell media comprised of: AIM V (Thermo Fisher), 5% fetal bovine serum (FBS) ...
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bioRxiv - Synthetic Biology 2023Quote: ... The supernatant was subsequently aspirated from the wells and 0.5x106 T cells were added in 1mL of T cell media comprised of: AIM V (Thermo Fisher), 5% fetal bovine serum (FBS) ...