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Citations for Thermo Fisher :
201 - 250 of 6074 citations for Peptide T since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... T cells were then stimulated with anti-CD3/anti-CD28 human T-Activator Dynabeads® (Invitrogen) at a 1:2 ratio of beads to T cells ...
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bioRxiv - Immunology 2023Quote: ... Primary human T cells were thawed and activated with Human T-Expander CD3/CD28 Dynabeads (Gibco) at a 3:1 bead:cell ratio in complete medium (RPMI 1640 supplemented with 10% fetal bovine serum ...
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bioRxiv - Immunology 2023Quote: ... Assessment of T Cell Proliferation: T cells were labeled with 5 μM Cell Trace CFSE (Invitrogen) as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... T-cells were activated using Dynabeads™ T-cell activator with CD3/CD28 (Thermofisher Inc., #11161D) for 1-2 days using directions provided by the manufacturer (1:1 bead to cell ratio) ...
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bioRxiv - Molecular Biology 2021Quote: ... pMA-T-ESAG1 was synthesised (Invitrogen) to contain 300 bp of sequence homologous to the region downstream of ESAG1 with and XcmI site 150 bp from either end ...
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bioRxiv - Cell Biology 2022Quote: HeLa T-REx cells (ThermoFisher Scientific) stably expressing LAP-Borealinwt ...
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bioRxiv - Cell Biology 2021Quote: ... High Five cells (T. ni, ThermoFisher) were grown in EX-CELL 405 medium (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... coated T-25 flask (Nunc Easyflask). Proliferation was maintained using HNSC.100 medium (composed of DMEM/F12 ...
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bioRxiv - Physiology 2020Quote: ... in T-PER (Thermo Fisher Scientific), supplemented with protease inhibitor cocktail (Promega ...
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bioRxiv - Cancer Biology 2022Quote: ... 293 Flp-In T-REx (Invitrogen) cells inducibly expressing N-terminally-tagged full length human C16orf72 or GFP-control were generated and inducible expression tested by immunoblotting ...
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bioRxiv - Molecular Biology 2020Quote: ... T-REx™-HeLa (Invitrogen™) and BJ cells (ATCC® CRL-2522™ ...
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bioRxiv - Cell Biology 2019Quote: ... HEK T-REx-293 cells (Invitrogen) were transfected with pcDNA4/TO-mPiezo1 using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... troponin-T (Thermo Scientific; #MA5-12960)] were added to each section and incubated for 90 min at RT ...
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bioRxiv - Biochemistry 2021Quote: HEK293 Flp-In T-Rex (Invitrogen), HeLa (Austin et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... 293 Flp-In T-REx (Invitrogen) cells inducibly expressing C-terminally-tagged full length human p53R273H ...
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bioRxiv - Molecular Biology 2022Quote: ... Flp-In T-REx 293 (Invitrogen), and U2OS cells (University of Helsinki ...
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bioRxiv - Immunology 2023Quote: ... T-bet (Invitrogen, 25-5825-80), RORγt (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: T-REx-U2OS (U2OStx; Life Technologies) and HEK293T (ATCC ...
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bioRxiv - Cell Biology 2023Quote: ... and oligo d(T)20 (Invitrogen) sequences ...
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bioRxiv - Immunology 2023Quote: ... Dynabeads human T-Activator (Thermo Fisher) anti-CD3/CD28 beads (at a 1:1 bead/cell ratio) ...
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bioRxiv - Biochemistry 2022Quote: T-REx™293 (Invitrogen, USA) and T-REx293 PEX13KO cells (Ott et al. ...
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bioRxiv - Cell Biology 2023Quote: ... CD45 (Thermo Fisher, A304-376A-T), or COL1A1 (R&D Systems ...
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bioRxiv - Plant Biology 2024Quote: ... oligo d(T)20 primers (Invitrogen) and 1 µg of RNA in a final reaction volume of 20 µl.
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Nucleolar Pol II interactome reveals TBPL1, PAF1, and Pol I at intergenic rDNA drive rRNA biogenesisbioRxiv - Molecular Biology 2023Quote: ... 293 T-RExTM cells (ThermoFisher Scientific) were maintained in DMEM supplemented with 10% (v/v ...
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bioRxiv - Molecular Biology 2021Quote: The peptide concentration was determined by using the Pierce Quantitative Colorimetric Peptide Assay (Thermo Fisher Scientific, Rockford, IL) and 180 µg peptides from each sample were loaded onto MacroSpin column (NestGroup ...
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bioRxiv - Immunology 2019Quote: ... containing LCMV peptides (1:1000, Peptide 2.0 Inc.) and Protein Transport Inhibitor Cocktail (1:500, Thermo Fisher Scientific). Cells were incubated for 4 hours at 37°C and then surface antigens were stained as described above ...
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bioRxiv - Microbiology 2021Quote: ... Labeled peptides of all samples were mixed equally and desalted by peptide desalting spin columns (Thermo Fisher, 89,852). A C18 column (Waters BEH C18 ...
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bioRxiv - Plant Biology 2022Quote: LC-MS/MS analysis of peptides and phosphopeptides – Peptides mixtures were analyzed by nanoflow Easy-nLC (Thermo Scientific) and Orbitrap hybrid mass spectrometer (Q-exactive HF ...
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bioRxiv - Systems Biology 2022Quote: ... Peptide concentrations were determined using a fluorimetric peptide assay kit following the manufacturer’s instructions (Thermo Scientific, Cat#23290).
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bioRxiv - Plant Biology 2019Quote: ... followed by peptide fractionation using the Pierce High pH Reverse-Phase Peptide Fractionation Kit (Thermo Scientific, Rockford, IL). Eight fractions of peptides from each sample were subjected to liquid chromatography-tandem mass spectrometry (LC-MS/MS ...
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bioRxiv - Cell Biology 2021Quote: ... Phospho-peptide enrichment was then performed using the High-SelectTM TiO2 Phospho-peptide Enrichment kit (Thermo Fisher Scientific). Each replicate was divided between two TiO2 columns and prepared according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Peptides were then desalted by reverse-phase chromatography using Pierce peptide desalting spin columns (Thermo Fisher part #89852). Final resuspension was done with Solvent A (0.1% formic acid in Fisher Optima LC/MS grade water ...
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bioRxiv - Plant Biology 2022Quote: ... Peptides were subsequently fractionated using high pH reversed-phase peptide fractionation kit (Thermo Fisher Scientific, San Jose, USA) and pooled into 8 fractions [43] ...
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bioRxiv - Biophysics 2023Quote: ... The concentration of peptide was determined using Pierce quantitative fluorometric peptide assay (Cat# 23290, Thermo Scientific, Rockford, IL). 11 – 18 μL of the peptides were analyzed through nano-ESI-LC/MS/MS using the Orbitrap Exploris mass spectrometer coupled to a Dionex nano-LC system (Thermo Scientific ...
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bioRxiv - Systems Biology 2023Quote: Phospho-peptide enrichment was carried out using the High-Select TiO2 Phospho-peptide Enrichment kit (Thermo Fisher, A32993), as per manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... The peptide concentration of this pool was determined using a fluorimetric peptide assay kit (Thermo Scientific, Cat#23290). Peptide concentrations per sample were estimated by multiplying the optical density recorded at harvest with the ratio between pool peptide concentration and the median at-harvest optical density.
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bioRxiv - Cancer Biology 2023Quote: The peptide amount in each sample was estimated using a quantitative colorimetric peptide assay kit (Thermo Fisher Scientific). Within each batch ...
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bioRxiv - Molecular Biology 2023Quote: ... The peptide mix was then fractionated using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (ThermoFisher Scientific). The 8 obtained fractions were analyzed by online nanoliquid chromatography coupled to MS/MS (Ultimate 3000 RSLCnano and Q-Exactive HF ...
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bioRxiv - Microbiology 2023Quote: ... of 78 of 15-mer peptides covering DBV GnH formulated at 0.625μg/ml for each peptide in RPMI medium (Gibco) in the 96-well plate ...
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bioRxiv - Cell Biology 2024Quote: ... soluble peptide suspension was transferred into fresh Eppendorf tubes and peptide concentration was determined by microBCA (Thermo Scientific). Equal amounts of peptides for each sample (150-200 µg ...
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bioRxiv - Genetics 2024Quote: ... The concentration of tryptic peptides was measured using the Pierce Quantitative Peptide Assays kit (ThermoFisher Scientific cat# 23275), and 25 µg of peptides were separated on a home-made reverse-phase C18 column in a pipet tip ...
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bioRxiv - Cell Biology 2019Quote: ... or HA peptide (Thermo Fisher Scientific, 26184).
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bioRxiv - Microbiology 2019Quote: ... Peptides were labelled with TMT6plex (ThermoFisher Scientific), desalted with solid-phase extraction on a Waters OASIS HLB µElution Plate (30 µm) ...
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bioRxiv - Cancer Biology 2020Quote: ... Eluted peptides were analyzed by Thermo Scientific Q Exactive or Q Exactive plus mass spectrometer utilizing a top 15 methodology in which the 15 most intense peptide precursor ions were subjected to fragmentation ...
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bioRxiv - Microbiology 2021Quote: ... All peptides were obtained from Thermo Fisher Scientific (Planegg ...
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bioRxiv - Cell Biology 2020Quote: ... Peptides were labelled with TMT10plex (Thermo Fisher) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Peptides were identified using SEQUEST (Thermo Scientific) and the results were compiled in Scaffold (Proteome Software) ...
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bioRxiv - Neuroscience 2022Quote: ... Peptides were coupled to SulfoLink Beads (Invitrogen) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: Two surrogate peptides purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Neuroscience 2020Quote: ... 13C-labelled AQUA peptides (Thermo Fisher Scientific) were synthesized to use as internal reference standards ...