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Citations for Thermo Fisher :
51 - 100 of 6074 citations for Nucleoside diphosphate kinase A NDPKA Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... using T4 polynucleotide kinase (Thermo Scientific). A 10 μl nuclease reaction mixture contained 20 μM wild-type Mmp-aCPSF1 protein or its catalytic inactive mutant ...
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bioRxiv - Molecular Biology 2023Quote: ... and T4 polynucleotide kinase (Thermo Scientific) followed by dephosphorylation using FastAP thermosensitive alkaline phosphatase from Thermo Scientific ...
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bioRxiv - Immunology 2023Quote: ... 100 units T4 kinase (Life Technologies) was added and incubated for 2 hours at 37°C for phosphorylation ...
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Atypical epigenetic and small RNA control of transposons in clonally reproducing Spirodela polyrhizabioRxiv - Plant Biology 2024Quote: ... using T4 Polynucleotide kinase (Thermofisher, #EK0031) (see Supplementary figures for a list of oligonucleotides used) ...
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bioRxiv - Immunology 2020Quote: Immunized mice were injected intravenously with 2 mg of the nucleoside analog EdU (Molecular Probes) in PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... Nucleosides were analyzed by LC-MS/MS on a Q-Exactive mass spectrometer (Thermo Scientific) fitted with a nanoelectrospray ion-source (Proxeon) ...
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bioRxiv - Microbiology 2021Quote: SARS-CoV-2 (438-516) S-RBD((HiS)6 and biotynilated human ACE2 have been purchased from Fisher Scientific (respective references 16534204 and 16545164) ...
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bioRxiv - Systems Biology 2021Quote: ... Tn5 underwent a His-tag (Dynabeads His-Tag Isolation & Pulldown, Invitrogen, 10103D) clean-up according to the manufacturer’s protocol to remove unbound oligos before using.
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bioRxiv - Microbiology 2021Quote: ... 10% HI FBS (Gibco), 1X ultraglutamine (Lonza) ...
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bioRxiv - Microbiology 2021Quote: ... His (ThermoFisher, MA1-21315), FLAG (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... anti-His mAb (Invitrogen) and anti-GAPDH mAb (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... HI FBS (2273356P, Gibco). HEPES (54457 ...
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bioRxiv - Immunology 2020Quote: ... or anti-His (Invitrogen) for recombinant ZVrecE80 antigen in 0.1M carbonate buffer pH 9.6 ...
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bioRxiv - Immunology 2020Quote: ... + 10% HI-FCS (Gibco) + 1% Peniciliin/Streptomycin (100x ...
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bioRxiv - Microbiology 2022Quote: ... anti-penta HIS (Invitrogen) and anti-HSP70 (26 ...
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bioRxiv - Molecular Biology 2023Quote: ... 10% HI-FBS (Gibco), 1% penicillin/streptomycin (Gibco) ...
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bioRxiv - Molecular Biology 2023Quote: ... 10% HI-FBS (Gibco), 1% penicillin/streptomycin (Gibco) ...
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bioRxiv - Molecular Biology 2023Quote: ... 10% HI-FBS (Gibco), 1% penicillin/streptomycin (Gibco) ...
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bioRxiv - Cancer Biology 2023Quote: ... and His-tag (Invitrogen) were used ...
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bioRxiv - Biochemistry 2022Quote: ... without nucleosides and supplemented with 10% fetal bovine serum (Hyclone for NIH3T3 cells, Gibco for others), 100 units/mL penicillin/streptomycin and 2 mM L-glutamine ...
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bioRxiv - Bioengineering 2020Quote: ... and minimum essential medium (α-MEM; GlutaMAX™ supplement, no nucleosides, Fisher Scientific, 11534466, the Netherlands) for hDFs and hMSCs ...
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bioRxiv - Cancer Biology 2021Quote: ... Melanoma cell lines were grown in MEMα (Minimum Essential Medium α, no nucleosides; 12561056, Gibco™) supplemented with 5 % fetal bovine serum ...
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bioRxiv - Biochemistry 2022Quote: ... The quantification of the nucleoside incorporation was performed with the Click-iT EdU microplate assay (Invitrogen, ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... The cells were grown in Minimum Essential Medium α (MEM α, nucleosides, no ascorbic acid, Gibco) media supplemented with 10% FBS ...
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bioRxiv - Genomics 2019Quote: ... 500 ng of in situ Hi-C libraries were first mixed with 2.5 μg human Cot-1 DNA (Invitrogen #15279011), 2.5 μg salmon sperm DNA (Invitrogen #15632011) ...
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bioRxiv - Bioengineering 2020Quote: ... T cells were cultured in RPMI-1640 supplemented with 10% HI-FBS and fed with recombinant human IL-2 (ThermoFisher) and IL-15 (Miltenyi ...
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bioRxiv - Immunology 2021Quote: ... The expression vectors encoding mHVEM (Q39-Q206) fused with human IgG1 and a subsequent hexa-His tag sequences were transfected into Expi293 (Gibco) cells using ExpiFectamine 293 transfection kit (Gibco ...
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bioRxiv - Immunology 2021Quote: ... The DNA sequences encoding a protein biologic composed of mHVEM residues (Q39- Q206) followed by human IgG1 and a subsequent hexa-His tag sequences were cloned into pcDNA 3.3 vector (Life technologies) using In-fusion HD cloning enzyme premix (Clontech) ...
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bioRxiv - Molecular Biology 2022Quote: ... zipper tag via a 5XGGS linker region was co-transfected with N-terminally His-tagged human Leptin in suspension HEK293 FreeStyle cells (ThermoFisher) in the presence of 20 μM kifunensine (Dextra ...
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bioRxiv - Systems Biology 2023Quote: ... T cells were expanded in RPMI-1640 media supplemented with 10% heat-inactivated FBS (HI-FBS) as well as recombinant human IL-2 (50 U/mL; ThermoFisher) and IL-15 (1 ng/mL ...
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bioRxiv - Microbiology 2020Quote: ... treated with T4 polynucleotide kinase (Thermo Fisher), and cloned into the AarI (Thermo Fisher ...
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bioRxiv - Biochemistry 2021Quote: ... phosphorylated using T4 polynucleotide kinase (Thermo Scientific) for 1 h at 37°C and annealed to phagemid ssDNA (90°C for 3 min ...
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bioRxiv - Plant Biology 2020Quote: ... and Polynucleotide Kinase (Thermo Fisher Scientific, USA). Additionally ...
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bioRxiv - Molecular Biology 2020Quote: ... and anti-phosphoglycerate kinase Pgk1 (459,250; Invitrogen) antibodies were employed to detect H3 histone ...
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bioRxiv - Molecular Biology 2021Quote: ... using T4 Polynucleotide Kinase (Thermo Fisher Scientific) before gel loading.
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bioRxiv - Systems Biology 2023Quote: ... 2.5 µL T4-polynucleotide kinase (Thermo Scientific), 2 µL SUPERase•In RNase Inhibitor and incubated for 10 min at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.5 µl T4-Polynukleotid-Kinase (ThermoFisher, EK0031) and 6.5 µl upH2O was mixed ...
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bioRxiv - Molecular Biology 2023Quote: ... and T4 polynucleotide kinase (Thermo Scientific, EK0031). M-MuLV Reverse Transcriptase (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... and T4 polynucleotide kinase (PNK, Thermofisher, #EK0032), washed twice with RIPA-S buffer and once with PNK buffer.
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bioRxiv - Microbiology 2023Quote: ... by using T4 polynucleotide kinase (Thermo Scientific) and purified with the NucleoSpin Gel and PCR Cleanup kit (Macherey-Nagel) ...
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bioRxiv - Cancer Biology 2022Quote: ... A CRISPR based kinome-wide screening was performed using a lentiviral sgRNA library (LentiArray™ Human Kinase CRISPR Library, Thermo Fisher Scientific, M3775) that knocks out 840 kinase and kinase related genes individually with total number of 3214 sgRNA constructs ...
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bioRxiv - Microbiology 2020Quote: ... Tsp-his was detected with the Anti-6X His tag antibody (ThermoFisher Scientific), which is already conjugated with HRP and does not require a secondary antibody for detection ...
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bioRxiv - Developmental Biology 2023Quote: ... For antibodies we used a His antibody (6x-His Tag Monoclonal Antibody, ThermoFisher), a MBP antibody (Anti-MBP Monoclonal Antibody ...
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bioRxiv - Immunology 2020Quote: Chloroquine diphosphate salt (CQ) (98%) and adenosine 5’-monophosphate sodium salt hydrate (AMP) (99%) were purchased from ACROS Organics™ ...
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bioRxiv - Cancer Biology 2020Quote: Kinase assays were performed using the Omnia Kinase Assay according to the manufacturer’s protocol (Life Technologies, Carlsbad, CA, USA); see Supplemental Methods.
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bioRxiv - Biophysics 2020Quote: The DNA sequences of the human CE GTase and each sequence variant were synthesised and subcloned into the PGEX6p1-C-His plasmid vector by Thermo Fisher GeneArt ...
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bioRxiv - Neuroscience 2021Quote: His-tagged human dynamin 1 was expressed using the Bac-to-Bac baculovirus expression system (Thermo Fisher Scientific, Waltham, MA, USA) and purified as described previously (66) ...
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bioRxiv - Immunology 2021Quote: ... were cloned into pCEP4 mammalian expression vector containing N-terminal human Ig kappa leader sequence and C-terminal Avi-tag and His-tag (Invitrogen, USA). Expi293-Freestyle cells cultured at 37°C and 8% CO2 in growth medium containing Expi293 Expression Medium at 3×106/mL in 50 mL media were transfected overnight at 37 °C with 50μg of plasmid in 160μL of ExpiFectamine plus 6mL of OptiMEM-I (all from Invitrogen) ...
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bioRxiv - Physiology 2021Quote: ... The human full-length BACE1 or BACE2 cDNAs were expressed from the pcDNA3.1/myc-His expression vector (Invitrogen, Waltham, MA, USA).
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bioRxiv - Molecular Biology 2021Quote: 0.63 mmole (1 equivalent) of the nucleoside-5’-monophosphate free acid (Santa Cruz Biotechnology or ACROS Organics) and 5 equivalents of 2-aminoimidazole hydrochloride (Combi-Blocks ...