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Citations for Thermo Fisher :
1 - 50 of 6074 citations for Nucleoside diphosphate kinase A NDPKA Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... nucleosides (ThermoFisher) supplemented with 2mM L-glut ...
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bioRxiv - Bioengineering 2021Quote: ... no nucleosides (Gibco), supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2020Quote: ... no nucleosides (Gibco). For the culture of C3H10T1/2 cells ...
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bioRxiv - Cancer Biology 2019Quote: HMF and MRC5 fibroblasts were transfected with a pool of 3 siRNAs targeting each of the 710 human kinases (Silencer Human Kinase siRNA Library, #A30079 Thermofisher). Fibroblasts (1250 cells/well ...
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bioRxiv - Cell Biology 2022Quote: ... 50 μM chloroquine diphosphate (C2301100G, Fisher Scientific), and10μM BI605906 (50-203-0195 ...
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bioRxiv - Bioengineering 2020Quote: ... α –MEM medium (+nucleosides, 22571-020, Gibco), fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2022Quote: ... the recombinant GST-tagged kinase active human mTOR (Cat. PV4753, ThermoFisher) was used instead of the immunoprecitated mTORC1 ...
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bioRxiv - Synthetic Biology 2023Quote: ... no nucleosides (Thermo Fisher Scientific, 12561-056), 100 mL Fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2019Quote: ... and MEMα (nucleosides, no phenol red; Gibco, 41061029) respectively ...
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bioRxiv - Microbiology 2020Quote: ... All human MARCH cDNAs were cloned into pCDNA3.1/myc-His A (Invitrogen) and were then subcloned into pBJ5 ...
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bioRxiv - Microbiology 2023Quote: ... human MARCH4 and mouse MARCH2 (mMARCH2) into pcDNA3.1/myc-His A (Invitrogen) and pBJ5 vector have been previously described (10) ...
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bioRxiv - Microbiology 2019Quote: ... and nucleoside analogues precursors were purchased from Acros Organics, Carbosynth ...
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bioRxiv - Bioengineering 2019Quote: ... consisting of αMEM with Glutamax and no nucleosides (Gibco) supplemented with 10 vol% FBS (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were cultured in αMEM containing nucleosides (Gibco, 12571-063) supplemented with 20% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2020Quote: ... extracellular signal-regulated kinase kinase (MEK, Ambion: Cat. no. s11167), Akt1 (Ambion ...
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bioRxiv - Bioengineering 2021Quote: ... CM was composed of αMEM with Glutamax and no nucleosides (Gibco) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Pathology 2019Quote: ... then washed once with culture media (MEM alpha with nucleosides [Gibco catalog #12571-063] + 10% heat-inactivated fetal bovine serum + 1X penicillin/streptomycin + 10 μg/mL gentamycin + 2 μg/mL ciprofloxacin + 2 μg/mL ceftazidime) ...
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bioRxiv - Cell Biology 2021Quote: ... with or without nucleosides (Thermo Fisher 32571-036 and 32561-037) with 10% dialyzed FBS (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... Full-length human LRRC4B was subcloned and inserted into the pcDNA6-V5-His vector (Invitrogen). FAM19A5 (NM_001252310.1 ...
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bioRxiv - Neuroscience 2024Quote: ... After blocking supernatant samples from different groups and standards (recombinant human-TREM2-His; Life Technologies) were incubated for 2 h at room temperature (RT ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Guanosine-5-diphosphate (GDP) and unlabeled GTPγS were purchased from Fisher Scientific (Waltham, MA) and Sigma-Aldrich (St ...
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bioRxiv - Systems Biology 2021Quote: ... and OCI-AML3 in α-MEM (with nucleosides; Gibco/Thermo Fisher Scientific) supplemented with GlutaMAX (Gibco CTS/Thermo Fisher Scientific) ...
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bioRxiv - Systems Biology 2021Quote: ... and OCI-AML3 in α-MEM (with nucleosides; Gibco/Thermo Fisher Scientific) supplemented with GlutaMAX (Gibco CTS/Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... phosphoglycerate kinase (PGK; Invitrogen), and glucose-6-phosphate dehydrogenase (G-6-PDH ...
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bioRxiv - Microbiology 2023Quote: ... and T4 kinase (Invitrogen), and purified on an 8% polyacrylamide/7M urea gel and eluted in buffer containing 20 mM Tris-HCl/pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... Lec2 and Lec2-NgR1 cells were maintained in MEM α nucleosides medium (Gibco) supplemented to contain 10% FBS ...
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bioRxiv - Genetics 2024Quote: MDCK cells (JCRB cell bank: IFO50071) were cultured in αMEM with nucleosides (Gibco) and 10% FBS (Gibco ...
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bioRxiv - Biochemistry 2020Quote: ... 6x-His-tagged constructs of human and murine SNED1 cloned into pCDNA5/FRT (Thermo Fisher, Waltham, MA) between the FseI and AscI sites were used to transiently transfect 293T cells to validate the anti-SNED1 antibody generated in this study (Supplementary Figure S1) ...
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bioRxiv - Biochemistry 2019Quote: ... using polynucleotide kinase (Thermo Scientific). Oligonucleotides were quantified spectophotometrically using calculated extinction coefficients (OligoAnalyzer tool ...
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bioRxiv - Cell Biology 2021Quote: ... α-phosphoglycerate kinase (Life Technologies), α-HA rabbit (Sigma) ...
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bioRxiv - Biophysics 2021Quote: ... and T4 polynucleotide kinase (Thermofisher). One pmol of labeled primer was mixed with one μl of ribonuclease reaction mix ...
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bioRxiv - Microbiology 2021Quote: ... and T4 polynucleotide kinase (Thermofisher) as described (Nguyen Le Minh et al. ...
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bioRxiv - Microbiology 2022Quote: ... with polynucleotide kinase (Thermo Scientific). Duplex DNA was obtained by annealing equimolar amount of complementary ssDNA in 10mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... 5 μg creatine kinase (Invitrogen) and 20U RNase inhibitor (NEB ...
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bioRxiv - Biochemistry 2024Quote: ... 5 μg creatine kinase (Invitrogen) and 20U RNase inhibitor (NEB) ...
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bioRxiv - Immunology 2023Quote: ... Expression constructs for M35-V5/His and M27-V5/His (both in pcDNA3.1-V5/His, Invitrogen) have been described previously (Munks et al ...
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bioRxiv - Biochemistry 2022Quote: ... recombinant human RPA or RPA-variants (2 μM) were incubated with 250 nM of recombinant Aurora B kinase (Invitrogen Inc.) in kinase reaction buffer (50 mM Tris-HCl ...
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bioRxiv - Bioengineering 2021Quote: The HHH peptide (Cys-biotin-PEG2-Gly-His-His-His-Gly(COOH)) was purchased from Thermo Fisher. It was 84% pure as determined by mass spectrometry ...
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bioRxiv - Genomics 2020Quote: ... and 1 μL of 10 μM de-oxy-nucleoside triphosphates (Invitrogen, Carlsbad, CA, USA) were treated for 5 min at 65°C and added to 10 μL reverse transcription master mix containing 200 U/μL M-MLV RT enzyme (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... MS-5 mesenchymal cells were grown in α-MEM medium w/o nucleosides (Gibco) supplemented with 10% fetal calf serum ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were cultured in growth media (α-Minimum Essential Medium (MEM) without nucleosides (Gibco) supplemented with 16% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: ... followed by nucleoside analysis using a Q Exactive Plus Hybrid Quadrupole-Orbitrap (Thermo Scientific) as previously described (39 ...
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bioRxiv - Molecular Biology 2024Quote: After dephosphorylation of nucleotides to nucleosides using shrimp alkaline phosphatase (ThermoFisher Scientific, Waltham, USA), LC-MS/MS measurements were performed ...
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bioRxiv - Molecular Biology 2024Quote: The cDNA sequence of human Sph1 was subcloned from the pcDNA3.1/V5-His-TOPO expression vector (Invitrogen, USA) 62 into the Venus-BiFC plasmids previously described 37 ...
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bioRxiv - Cancer Biology 2022Quote: ... (FBS HI; Invitrogen) and 1% Glutamax (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... anti-His (Invitrogen), or anti-FLAG (Sigma ...
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bioRxiv - Cell Biology 2023Quote: Methods used for these assays have been described.28 Briefly, human FLAG-tagged CDKL5 WT or kinase dead (KD, CDKL5 K42R) constructs were subcloned into pT7CFE1-CHis plasmid (Thermo Fisher). These constructs were then used for in vitro translation using a HeLa cell lysate-based Kit (1-Step Human Coupled IVT Kit—DNA ...
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bioRxiv - Neuroscience 2023Quote: ... FLAG-tagged WT or kinase dead (KD, CDKL5 K42R) constructs of human CDKL5 were subcloned into a pT7CFE1-CHis plasmid (Thermo Fisher). A HeLa cell lysate-based Kit (1-Step Human Coupled IVT Kit—DNA ...
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bioRxiv - Cell Biology 2021Quote: Kinase activity assays were performed using the SelectScreen biochemical kinase profiling service provided by Life Technologies. Adapta screening protocols were used to measure PIK3C2A (PtdIns3K-C2α) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Guanosine-5-diphosphate (GDP) and guanosine 5’-O-[gamma-thio]triphosphate (GTPγS) were purchased from Fisher Scientific and Sigma-Aldrich ...