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Citations for Thermo Fisher :
351 - 400 of 10000+ citations for Mumps virus lysate Isolate 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... pneumoniae isolates were stored at −80°C in Luria-Bertani broth (LB; Thermo Fisher Scientific, Waltham, MA) supplemented with 15% glycerol.
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bioRxiv - Immunology 2021Quote: SARS-CoV-2 isolates were propagated in VeroE6 cells in Opti-MEM I (Invitrogen, Carlsbad, CA, USA) containing 0.3% bovine serum albumin (BSA ...
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bioRxiv - Genomics 2021Quote: RNA was extracted for each isolate with the Ambion Purelink RNA Kit (Thermo Fisher Scientific, Waltham, MA). Briefly ...
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bioRxiv - Genetics 2020Quote: ... Total RNA was subjected to isolate Poly (A) mRNA with poly-T oligo attached magnetic beads (Invitrogen). RNA fragments were reverse-transcribed to create the final cDNA libraries following the NEBNext® Ultra™ RNA Library Prep Kit (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... DNA extraction was performed on the isolates using the MagMAX Multi-sample DNA extraction kit (ThermoFisher Scientific) as per the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... and the cleared lysates were incubated with 1 mL HisPur Ni-NTA Resin (Thermo Fisher Scientific), washed and eluted ...
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bioRxiv - Neuroscience 2020Quote: ... the lysates were incubated for 1 hour with 2 μg anti-GFP antibody (Thermo Fisher Scientific) and then incubated at 4°C for 30-60 minutes with Protein A Dynabeads (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ERα proteins were immunoprecipitated from J774a.1 cell lysates according to the manufacturer’s protocols (11204D, Invitrogen). The ERα antibody was the same as that used for Western blotting and was purchased from Santa Cruz (1:200 dilution) ...
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bioRxiv - Microbiology 2021Quote: ... Viral lysate was diluted 1:10 by adding 90 μl of nuclease-free water (Life Technologies). qRT-PCR reactions were prepared by mixing 9.6 μl of diluted viral lysate with 10.4 μl of reaction mix containing 10 μl of 2x Maxima SYBR Green/ROX qPCR Master Mix (ThermoFisher) ...
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bioRxiv - Developmental Biology 2019Quote: ... Lysates were prepared for Western blot by adding 1× NuPAGE LDS Sample Buffer (Thermo Fisher Scientific) with 1% β-mercaptoethanol and incubated at 95ºC for 5 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... The lysates were injected (350ul per injection) onto MAbPac SEC-1 (Thermo Scientific, 5μm, 300 × 4.0mm) or Superose 6 10/300GL column (GE Life Sciences) ...
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bioRxiv - Biochemistry 2021Quote: ... The cell lysate was centrifuged for 1 h at 40,000 rpm in T647.5 rotor (Thermo Scientific) to sediment insoluble material ...
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bioRxiv - Biochemistry 2021Quote: ... the precleared lysate was mixed with 1 mL glutathione-agarose beads (Thermo Fisher Scientific, Waltham, MA) and incubated for 1 h rotating at 4°C ...
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bioRxiv - Genomics 2022Quote: ... The homogenized lysate was filtered through a 40 μm Falcon cell strainer (ThermoFisher #08-771-1) into a 50 mL Falcon tube ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were made using RIPA buffer (supplemented with 1% SDS, HALT protease inhibitor (Thermo Fisher Scientific), and Benzonase ...
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bioRxiv - Neuroscience 2023Quote: ... A HeLa cell lysate-based Kit (1-Step Human Coupled IVT Kit—DNA, 88881, Life Technologies) enabled in vitro translation of these constructs ...
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bioRxiv - Molecular Biology 2023Quote: ... lysates equivalent to 10 million cells were incubated with 1 uL of RNAse T1 (ThermoFisher Scientific) for 40 minutes at RT before reaction was quenched on ice with 200 U of SuperaseIN (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... N-term B virus mut) using a Neon® Transfection System (Invitrogen) under the recommended electroporation condition (1350 V ...
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bioRxiv - Biochemistry 2019Quote: ... Recombinant virus was made by co-transfection into SF9 insect cells (Invitrogen) of the plasmid and BacVector3000 baculovirus DNA (Novagen ...
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bioRxiv - Systems Biology 2019Quote: ... and MMLV-RT reverse transcriptase (Moloney Murine Leukemia Virus, Invitrogen Life Technologies), and the reaction was carried according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2019Quote: ... and MMLV-RT reverse transcriptase (Moloney Murine Leukemia Virus, Invitrogen Life Technologies), and the reaction was carried according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... The virus samples were treated with 300 units of RNase I (Ambion) for 30 min followed by SUPERaseI RNase Inhibitor (Thermo Fisher ...
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bioRxiv - Cancer Biology 2019Quote: ... 2.5mL virus with 10μg/mL polybrene (Thermo Fisher Scientific TR-1003-G) was added to 5×105 cells plated in 6-well dishes ...
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bioRxiv - Microbiology 2020Quote: ... The virus was rescued and propagated in T-REx-293 cells (Invitrogen). Purification was by CsCl gradient ultracentrifugation ...
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bioRxiv - Cancer Biology 2020Quote: ... Concentrated virus was filtered using 0.45um syringe filters (ThermoFisher; Cat#: 725-2545) and immediately added to cultured OSE in 24-well plates ...
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bioRxiv - Microbiology 2020Quote: ... Stocks of virus were grown in Vero-E6 cells in DMEM (Gibco) supplemented with 10% fetal bovine serum (Atlas Biologicals ...
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bioRxiv - Microbiology 2020Quote: ... the virus particles were resuspended in CO2-independent media (ThermoFisher, Cat# 18045088), aliquoted ...
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bioRxiv - Immunology 2019Quote: ... purified virus was immobilized on 96-well maxisorp microtiter plates overnight (Nunc). Wells were blocked with 0.05% PBST [0.05% Tween-20 (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... c-MYC and KLF4 in CytoTune Sendai virus vectors (Thermo Fisher Scientific) (Sontag et al. ...
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bioRxiv - Microbiology 2021Quote: ... The virus was rescued and propagated in T-REx-293 cells (Invitrogen). Purification was by CsCl gradient ultracentrifugation ...
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bioRxiv - Immunology 2021Quote: ... Vero cells were used to propagate the virus in DMEM medium (Gibco) supplemented with 1% penicillin/streptomycin (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... Virus was removed from cells and replaced with post-infection media (Gibco OptiMem supplemented with 0.01% FBS ...
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bioRxiv - Biophysics 2022Quote: ... X-31 influenza virus was diluted in Opti-MEM (Gibco, cat # 11058021) at a ratio of 1:10000 to add 250 µL into each well (25) ...
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bioRxiv - Neuroscience 2023Quote: ... The virus-containing pellet was resuspended in 200 μL of DPBS (ThermoFisher) and stored at -80 °C ...
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bioRxiv - Microbiology 2023Quote: ... Each virus was mixed with PBS-washed defibrinated sheep blood (Fisher Scientific) containing 5 mM ATP to a concentration of 106 PFU/mL and fed to ∼50-60 female mosquitoes ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA synthesis was performed with Moloney murine leukemia virus reverse transcriptase (Invitrogen) at 38°C for 60 min ...
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bioRxiv - Neuroscience 2019Quote: ... 1 mg of striatal lysates was pre-cleared for 1 h with 80 ul Protein G Sepharose coated beads (ThermoFisher Scientific), then centrifuged at 4°C for 5 min at 1000 rpm ...
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bioRxiv - Molecular Biology 2023Quote: ... cleared lysate (1 mL) was mixed with 1 µg of relevant antibody (Mouse IgA isotype control (eBioscience Invitrogen 14-4762-81) and Mouse IgA anti-AGO2 (Santa-Cruz ...
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bioRxiv - Microbiology 2021Quote: ... Viral RNA was measured by quantitative real real-time polymerase chain reaction (qRT-PCR) using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) with primers and probe specific for the CCHFV L gene.
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bioRxiv - Microbiology 2020Quote: ... and quantitative real-time polymerase chain reaction (qRT-PCR) was conducted using TaqMan Fast Virus 1-Step Master Mix (Thermofisher Scientific, US) with primers and probe specific for the SARS-CoV-2 E gene following guidelines by the World Health Organization (https://www.who.int/docs/default-source/coronaviruse/wuhan-virus-assayv1991527e5122341d99287a1b17c111902.pdf ...
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bioRxiv - Microbiology 2021Quote: ... HCV RNA was quantified by one-step reverse transcription-quantitative PCR using 50 ng of total intracellular RNA and TaqMan® Fast Virus 1-Step Master Mix (Applied Biosystems) with primers and probe targeting the HCV 5’ nontranslated region as described previously [82] ...
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bioRxiv - Microbiology 2022Quote: The ZEBOV RT-PCR assay was performed in a 25 µL reaction mixture containing TaqMan Fast Virus 1-step master mix (Thermo Fisher Scientific), 5 µl template RNA ...
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bioRxiv - Microbiology 2021Quote: ... RNA extracted from animal samples or RNA standards were then measured using TaqMan Fast Virus 1-Step Master Mix (ThermoFisher, catalog # 4444432) and custom primers/probes targeting the E gene sgRNA (forward primer ...
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bioRxiv - Genetics 2022Quote: ... 30 μg of the virus) was digested with 1:100 (w/w) ratios of trypsin (Pierce Trypsin Protease mass spectrometry grade; Thermo Fisher Scientific)/virus for various time points at 25°C (31 ...
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bioRxiv - Plant Biology 2022Quote: ... Reverse transcription (RT) of the extracted RNA samples (1 µg) was performed using AMV (Avian Myeloblastosis Virus) Reverse Transcription Kit (Invitrogen, Waltham, USA) and oligo-dT18 (1 µM ...
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bioRxiv - Neuroscience 2023Quote: ... from the Stanford Gene Vector and Virus core unless otherwise specified) and Ctb retrograde tracers (1 ug/ul; Invitrogen #C34777, #C34776, #C34775) were infused with a syringe pump (Harvard Apparatus ...
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bioRxiv - Microbiology 2023Quote: ... The pooled libraries were mixed with virus-specific 161 biotinylated probes in the presence of human Cot-1 DNA (Invitrogen, Cat#15279011) and xGen Universal Blocking Oligos (IDT ...
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bioRxiv - Immunology 2022Quote: ... and subjected to the one-step quantitative real-time reverse transcription-PCR assay (qRT-PCR) using TaqMan Fast Virus 1-Step Master Mix (ThermoFisher, Cat# 4444432) as described previously58 ...
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bioRxiv - Microbiology 2023Quote: ... virus was serially diluted in Vero maintenance media (MEM supplemented with 2% FBS and 1% each of GlutaMax (Gibco, Grand Island, NY), PenStrep ...
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bioRxiv - Microbiology 2023Quote: ... First-strand cDNA synthesis was performed on 1 µg of total RNA with the RevertAid H Minus Moloney murine leukemia virus (M-MuLV) reverse transcriptase (Thermo Fisher Scientific) using random primers p(dN)6 (Roche) ...