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Citations for Thermo Fisher :
551 - 600 of 10000+ citations for Mumps virus lysate Isolate 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: IPS cells were derived from skin fibroblasts using Cytotune Sendai virus kit (Thermofisher, France) and cultured on MEF in KO- DMEM supplemented with KO-SR (Thermofisher ...
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bioRxiv - Microbiology 2023Quote: ... RNA was tested with TaqMan™ Fast Virus One-Step Master Mix (Applied Biosystems) using QuantStudio 6 or 3 Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Cell Biology 2024Quote: Elephas maximus endothelial and epithelial cells were transduced with Sendai virus (Thermofisher Scientific, A16517) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Abelson virus-transformed pre-B cells were maintained in DMEM (Thermo Fisher, 11960-077) supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... along with psPAX2 and pMD2.G packaging and envelope virus using lipofecctamine 3000 (Invitrogen) in serum-free Opti-MEM media (Invitrogen) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... A 5-μg sample of total RNA was used to isolate poly(A) mRNA with a poly(T) oligo primer attached to magnetic beads (Invitrogen). Briefly ...
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bioRxiv - Genetics 2022Quote: ... A Dynabeads™ mRNA Purification Kit (for mRNA purification from total RNA preps) was used to isolate mRNA (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... Approximately 10 μg of total RNA was subjected to isolate Poly (A) mRNA with poly-T oligoattached magnetic beads (Invitrogen). The poly(A)-or poly(A)+ RNA fractions is fragmented into small pieces using divalent cations under elevated temperature ...
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bioRxiv - Immunology 2021Quote: ... Each dilution was incubated at 37°C and 5% CO2 for 1 hour with an equal volume of 1000 plaque forming units/ml (PFU/ml) of SARS-CoV-2 (isolate USA-WA1/2020) diluted in DMEM (Gibco) containing 2% fetal bovine serum (Gibco ...
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bioRxiv - Microbiology 2020Quote: ... aureus isolates were confirmed by Gram staining followed by a positive catalase and Staphaurex latex agglutination test (Thermo Fisher Scientific). All isolates were stored at −80°C ...
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bioRxiv - Microbiology 2019Quote: DNA from each isolate was normalized to 0.2 ng/uL based on Qubit2 measurement (Thermo Fisher Scientific, Waltham, MA, USA) and used to prepare NexteraXT sequencing library (Illumina ...
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bioRxiv - Immunology 2021Quote: Serial dilutions of sera and BALs were incubated with 2000 PFU of an early SARS-CoV-2 isolate (GISAID EPI ISL 406862 Germany/BavPat1/2020) in 100 µl OptiPro medium supplemented with 1x GlutaMAX (both Gibco) for 1h at 37°C ...
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bioRxiv - Developmental Biology 2020Quote: ... Plasmid clone isolates of amplicon DNA were prepared using a Zero Blunt™ TOPO™ PCR cloning kit (K287520, Invitrogen) and their inserts were sequenced ...
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bioRxiv - Microbiology 2020Quote: ... Amplification products were purified using an Isolate II PCR and gel kit according to manufacturer’s instructions (Invitrogen, Carslbad, California, USA). Sanger sequencing of both strands of each PCR product was carried out commercially ...
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bioRxiv - Cell Biology 2021Quote: ... Embryo bodies were minced and digested with trypsin to isolate MEFs that were cultured in DMEM supplemented with 10% FBS (Gibco) and 1% penicillin and streptomycin ...
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bioRxiv - Microbiology 2022Quote: ... Vero E6 cells were infected with the SARS-CoV-2 wild type (isolate SARS-CoV-2/human/SWE/01/2020; Genbank accession: MT093571) in VP-SFM (Gibco). The virus was harvested at day three ...
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bioRxiv - Neuroscience 2022Quote: Prion isolates were prepared separately as 20% (w/v) brain homogenates in phosphate-buffered saline pH 7.4 (PBS; Life Technologies, Gibco) using the MP Biomedicals fast prep-24 homogenizer (Fisher) ...
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bioRxiv - Microbiology 2023Quote: ... 5 µL of each isolate sample were added to 10 µL of TaqMan Fast Advanced Master Mix solution (Thermo Fisher) with the corresponding primers and probes ...
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bioRxiv - Physiology 2023Quote: ... and clinical CRS-isolates (Drs. N. Cohen and L. Chandler, Philadelphia VA Medical Center [56]) were grown in Luria broth (Gibco). For anti-bacterial assays ...
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bioRxiv - Biochemistry 2023Quote: BAP1/ASXL1 and BAP1 were expressed in SF9 insect cells (clonal isolate from PLB-Sf-21-AE1) following the Bac-To-Bac Baculovirus Expression System established protocol (Invitrogen). ASXL1 was expressed in E ...
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bioRxiv - Genomics 2023Quote: Antimicrobial susceptibility of Salmonella isolates was determined using the Sensititre™ semi-automated system (Thermo Fisher Scientific, Kansas City, KS) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Neurons from K18-hACE2 and WT mice were inoculated with SARS-CoV-2 isolate USA-WA1/2020 at 30 MOI in 100 µL Neurobasal A (Invitrogen) for 8-well chamber slides and 200 µL for 24-well plates for 1 h ...
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bioRxiv - Microbiology 2023Quote: Whole genome shotgun sequencing was carried out separately for all the individual isolates using the Ion S5 (Thermo Fisher Scientific) and MinION (Oxford Nanopore Technologies ...
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bioRxiv - Immunology 2024Quote: ... Serum serial dilutions were incubated with 2000 PFU of an early SARS-CoV-2 isolate in 100 µl OptiPro medium supplemented with 1x GlutaMAX (both Gibco) for 1 h at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... Three biological replicates of each clinical isolate were grown overnight on Mueller-Hinton agar with antibiotics disc (Thermo Fisher Scientific). The measurements in mm were determined and calculated as an average of 3 readings and compared to the published EUCAST breakpoints to determine if the clinical isolate is sensitive ...
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bioRxiv - Microbiology 2021Quote: ... half of the lysate/VPM or lysate/PBS mixtures were subjected to a buffer exchange using a 100kDa MWCO protein concentrator (Pierce, Thermo Scientific), reduction to 20% volume and two washes with PBS prior to serial dilution and inoculation of Vero E6 cells as described above.
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bioRxiv - Cell Biology 2020Quote: For immunoprecipitation whole cell lysates or fractionated cytoplasmic lysates were incubated with indicated antibodies and Magnetic A/G beads (Thermo Scientific) or GFP-trap overnight at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Each half of diluted lysate is incubated with 1/10 volume of antibody coated Protein G Dynabeads™ (Invitrogen) for 40 min at 4°C by end to end rotation ...
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bioRxiv - Neuroscience 2021Quote: ... An equal amount (500µg) of each protein lysate was precleared for 1 hour with magnetic Protein G Dynabeads (Invitrogen) and immunopurified with 40µl washed anti-V5 agarose beads (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... Purified GST-Pak1PBD (20 μg) was added to 1 ml of 1.0 mg/ml lysates along with 15 μl of glutathione resin (ThermoFisher) and rotated for 90 min at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... lysate from mock transfected cells was incubated at 4°C for 1 hour with Sepharose-G beads (Fisher Scientific), previously washed with 5% BSA in TBS-Triton buffer and preincubated with 2.5 μg anti-GFP antibody ...
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bioRxiv - Microbiology 2021Quote: ... 1 ml of each ultrapurified phage passage lysate was treated with 10 μl Ambion™ DNase I (ThermoFisher Scientific) and 20 μl RNase (Sigma-Aldrich ...
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bioRxiv - Genetics 2020Quote: ... The chromatin lysates (1ml per 100mg cells) were precleared with C-1 magnetic beads (Dynabeads Myone streptavidin C1 [Invitrogen]) before incubation with probes ...
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bioRxiv - Immunology 2022Quote: ... complete protease inhibitor cocktail) and the cleared cell lysates were treated with 1 U/µl RNase T1 (ThermoFisher, EN0541). GFP-tagged SAMHD1 proteins were immunoprecipitated with anti-SAMHD1 antibody (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... Nucleic acids were extracted twice from lysates using Phenol/Chloroform/Isoamyl Alcohol 25:24:1 (ThermoFisher Scientific Cat#10308293) treatment followed by centrifugation at 8,000 g for 5 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... the lysates were incubated in shake tubes over 1 h at 4°C with recombinant protein G–Sepharose (Invitrogen). The Sepharose was washed with RIPA lysis buffer and eluates were obtained through an addition of Laemmli Buffer.
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bioRxiv - Microbiology 2023Quote: ... Skin lysates were diluted 1:10 in ProcartaPlex Universal Assay Buffer prior to use in custom Procartaplex Immunoassay (ThermoFisher) analyzed using the FlexMap3D (Luminex) ...
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bioRxiv - Cell Biology 2023Quote: ... lysates were separated by SDS-PAGE gel electrophoresis on 1 mM NuPAGE 4 – 12% bis-tris gels (Thermo Fisher) using MOPS running buffer and transferred to nitrocellulose membranes using Tris-Glycine-methanol transfer buffer (30.3 g Tris Base ...
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bioRxiv - Microbiology 2024Quote: ... Loading control for cell lysate was detected with anti-α-tubulin (rabbit polyclonal, Cat# PA1-20988, Invitrogen, 1:5,000) and for virus lysate was detected with anti-p24 (mouse monoclonal ...
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bioRxiv - Microbiology 2021Quote: Vero81 cells were adsorbed with CHIKV strains diluted in virus dilution buffer (VDB, RPMI medium with 25 mM HEPES [Gibco] supplemented to contain 1% FBS) at a multiplicity of infection of 0.01 plaque-forming units (PFU)/cell at 37°C for 1 h ...
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bioRxiv - Microbiology 2020Quote: ... Pyrene-labeled virus was produced in BHK-21 cells cultured beforehand in the presence of 15 μg/ml 1-pyrenehexadecanoic acid (Thermo Fisher Scientific, Waltham, MA, USA). The supernatant was harvested at 48 hpi ...
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bioRxiv - Genomics 2023Quote: ... Infiltrated leaves were collected 5 days after infiltration and homogenised with virus inoculation buffer (10 mM monopotassium phosphate containing 1% Celite (Thermo Fisher Scientific, 68855-54-9)) ...
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bioRxiv - Cancer Biology 2021Quote: Cell lysates were extracted using Triton X-100 lysing buffer and lysate was quantified using Pierce BCA Protein Assay Kit (ThermoFisher Scientific – 23225). 20 μg of protein for each cell lysate was run on a 4-15% SDS gel (Bio-Rad – 4561083DC ...
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bioRxiv - Genomics 2020Quote: ... We placed each 600-cell microdissected isolate into an empty cap of a nuclease-free 0.2 ml Axygen PCR tube (Thermo Fisher Scientific). We took photomicrographs both before and after LCM.
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bioRxiv - Biochemistry 2019Quote: The human embryonic kidney (HEK) 293FT cells a high transfection efficiency isolate of the HEK293T cells were purchased from ThermoFisher (# R70007), and used without further authentication ...
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bioRxiv - Microbiology 2019Quote: ... MIC for isolates were determined by broth microdilution using the Sensititre semi-automated antimicrobial susceptibility system (Thermo Fisher Scientific, Waltham, MA). Results were interpreted according to Clinical and Laboratory Standards Institute (CLSI ...
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bioRxiv - Microbiology 2021Quote: ... DNA extraction was performed from isolate cultures grown overnight on Sheep Blood Agar by using the MagMax DNA Multi-Sample extraction kit (ThermoFisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... was performed for genomic DNA that was extracted from cultures of bacterial isolates using the Easy-DNA Extraction Kit (Invitrogen®). Short-read WGS was performed using the Illumina MiSeq platform (Illumina Inc.) ...
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bioRxiv - Microbiology 2021Quote: All Salmonella isolates were tested for antimicrobial susceptibility against 14 class-representative antimicrobial agents using the Sensititre CMV4AGNF plate (Thermo Scientific), including ...
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bioRxiv - Pathology 2020Quote: ... the aAs isolates were grown on tryptone soya agar (TSA, Oxoid, UK) or blood agar (BA; TSA + 5% sheep blood Thermo Fisher) while the Vibrionaceae isolates were on sea water agar (SWA ...