Labshake search
Citations for Thermo Fisher :
101 - 150 of 10000+ citations for Mouse Procollagen II C Terminal Propeptide PIICP CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... Cys469 in the C-terminal DNase domain of these ColE9 constructs was labelled with a 3-fold Alexa Fluor 488-maleimide (Invitrogen) as previously described (41) ...
-
bioRxiv - Immunology 2022Quote: ... followed by an enterokinase cleavage site and a C-terminal double strep-tag was cloned into a modified pMT/BiP expression vector (pT350, Invitrogen). Drosophila S2 cells were stably co-transfected with pT350 and pCoPuro (for puromycin selection ...
-
bioRxiv - Biochemistry 2020Quote: ... the opsin-encoding constructs were fused in frame with a C-terminal 8-His tag and subcloned into the pPIC9K (A1ACR1, HfACR1 and AlACR2) or pPICZα (AlACR3) vector (Invitrogen) according to the manufacturer’s instructions.
-
bioRxiv - Neuroscience 2020Quote: BHK cells were electroporated with RNA of pSinRep5-HA-GluA1 or the C-terminal mutants and the helper DH (26S) according to Sindbis Expression System manual (Invitrogen) and as previously described (Osten et al. ...
-
bioRxiv - Genetics 2020Quote: ... constructs for dACE2 with C-terminal Myc-DDK and GFP tags cloned in pcDNA3.4 vector were custom-synthesized by Thermo Fisher. ACE2 with C-terminal GFP-tag (RG208442 ...
-
bioRxiv - Cell Biology 2021Quote: ... Parental cells expressing the prey-GFP were constructed in the BY4741 genetic background or obtained from the genomic C-terminal GFP fusion collection (ThermoFisher). Excluding those strains harboring plasmids that code for Drs2-GFP and drs2-5A-GFP ...
-
bioRxiv - Biophysics 2020Quote: ... with an N-terminal gp67 signal peptide for secretion and a C-terminal 6×His tag for purification was inserted into pFastBac-Dual vector (Invitrogen). The construct was transformed into bacterial DH10Bac component cells ...
-
bioRxiv - Cell Biology 2021Quote: The full-length cDNA of plexin-A2 was cloned into the gateway pDonor221 plasmid and then transferred by recombination into the pLenti6/V5-DEST lentiviral expression vector in frame with a C-terminal V5 tag according to the instructions of the manufacturer (Invitrogen), as previously described (Sabag et al. ...
-
bioRxiv - Microbiology 2022Quote: ... These constructs were transferred to the destination vector pK7FWG2 (Karimi, Inzé et al., 2002) for C-terminal GFP fusion using Gateway LR Clonase Enzyme Mix (Invitrogen).
-
bioRxiv - Biochemistry 2022Quote: ... with a N-terminal FLAG-tag and a C-terminal 6×His-tag preceded by a two-residues linker were subcloned into pFastBac 1 vector (Gibco). Sequence transposition into bacmid DNA using DH10Bac cells (Gibco ...
-
bioRxiv - Biochemistry 2022Quote: ... elegans) containing a C-terminal His6-tag was cloned into the polyhedrin promotor site of the pFastBac DUAL vector (Invitrogen). Baculoviruses for respective constructs were produced by transfecting Sf9-cells (Expression Systems ...
-
bioRxiv - Molecular Biology 2023Quote: Cytosolic domains for STING (consisting of either the C-terminal 141-341aa or 155-341aa) were cloned into the pcDNA3.1(+) (Thermo Fisher) vector backbone with a C terminal GFP tagging ...
-
bioRxiv - Molecular Biology 2022Quote: ... fused to a C-terminal trimeric GCN4 (tGNC4) zipper tag via a 5XGGS linker region was secreted from suspension HEK293 FreeStyle cells (ThermoFisher) in the presence of 20 μM kifunensine (Dextra ...
-
bioRxiv - Microbiology 2023Quote: ... with a secretion sequence and C-terminal His6-tag) were transiently expressed using FreeStyle™ 293-F cells (Thermo Fisher) in FreeStyle™ F17 Expression Medium supplemented with L-glutamine and 1X MEM non-essential amino acids (Gibco) ...
-
bioRxiv - Molecular Biology 2023Quote: ... and D1-D4 (domains 1-4) with C-terminal 6x-His or StrepII tags were transiently transfected using the Expi293 expression system (ThermoFisher)7 ...
-
bioRxiv - Microbiology 2022Quote: ... sE genes with a tandem C-terminal strep-tag in pMT/BIP/V5 plasmid were expressed in Drosophila S2 cells (Invitrogen) as described previously (20) ...
-
bioRxiv - Evolutionary Biology 2023Quote: Codon-optimised sequences of Homo sapiens and Callithrix jacchus LEUTX with a GGGGSGGGGS linker and C-terminal V5 tag (Supplementary Figure S5) were synthesised by ThermoFisher GeneArt and cloned into a pcDNA3.1 mammalian expression vector ...
-
bioRxiv - Physiology 2023Quote: ... followed by C-terminal region of human FGF-21 (amino acids 166-209) into a vector pCEP4 (Thermo Fisher Scientific). The construct was then transiently transfected to Expi293F cells (Thermo Fisher Scientific ...
-
bioRxiv - Plant Biology 2023Quote: ... the entry vector was subsequently recombined into the Gateway-compatible plant binary vectors pGWB505(48) for C-terminal fusion with EGFP (Enhanced Green Fluorescent Protein) through Gateway LR reaction (Invitrogen). The 1053 bp native promoter ...
-
bioRxiv - Cell Biology 2024Quote: ... and transfected with a total of 2 μg C-terminal EGFP tagged M2 tail using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer protocol after washing with 200 μL PBS and refreshing with 100 μL DMEM (ScienCell ...
-
bioRxiv - Bioengineering 2021Quote: ... Affymetrix® GeneChip® WT Terminal Labeling Kit Assay (Affymetrix, Inc., Santa Clara, CA, USA) was used for fragmentation and labeling ...
-
bioRxiv - Molecular Biology 2020Quote: ... using APO-BrdU terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) Assay Kit (Invitrogen) and lactate dehydrogenase (LDH ...
-
bioRxiv - Biophysics 2023Quote: ... were grown at 27 °C in Sf-900 II SFM medium (Gibco) supplemented with 5% (v/v ...
-
bioRxiv - Biophysics 2023Quote: ... were grown at 27 °C in Sf-900 II SFM medium (Gibco) supplemented with 5% (v/v ...
-
bioRxiv - Neuroscience 2019Quote: ... the SuperScript II Reverse Transcriptase kit (Invitrogen) was used ...
-
bioRxiv - Developmental Biology 2020Quote: ... and SuperScript II Reverse Transcriptase kit (Invitrogen). RT-qPCRs were performed in 96-well plates on ABI Prism 7500 (Applied Biosystems ...
-
bioRxiv - Molecular Biology 2021Quote: ... Using the DECAprime™II kit (Invitrogen) the PCR fragment was radioactively labelled with α-32P-dCTP ...
-
bioRxiv - Plant Biology 2024Quote: ... using the LR Clonase II Kit (Invitrogen). For N-terminal fusions with the GAL4-activation or -binding domains the vectors pGADT7-GW and pGBKT7-GW (gift from Yuhai Cui ...
-
bioRxiv - Developmental Biology 2024Quote: ... SuperScript II Reverse Transcription kit (Life Technologies) was used to generate cDNA ...
-
bioRxiv - Cell Biology 2024Quote: ... Superscript® II DNA synthesis kit (Invitrogen) was used to reverse-transcribe 500 ng to 1 µg of total RNA and synthesise cDNA ...
-
bioRxiv - Microbiology 2021Quote: ... mouse anti-c-myc (mAb 9E10, Life Technologies), mouse anti-acetylated Tubulin (mAb 6-11B-1 ...
-
CAP1 and cofilin1 cooperate in neuronal actin dynamics, growth cone function and neuron connectivitybioRxiv - Neuroscience 2020Quote: ... mouse anti-c-Myc (1:200, ThermoFisher Scientific) and chicken anti-mCherry (1:500 ...
-
bioRxiv - Microbiology 2022Quote: ... and expression clones with N-terminal fusion tags were produced simply by Gateway cloning (Gateway™ LR Clonase™ II Enzyme mix, Invitrogen, 11791020). The Lenti-NSP2 was constructed by using the destination vector ...
-
bioRxiv - Bioengineering 2019Quote: ... The montaged image in Figure 5 C ii and 5 C iii were generated by stitching together individual images using MAPS 1.1 software (ThermoFisher).
-
bioRxiv - Neuroscience 2020Quote: ... were designed against the mouse Auts2 coding sequence using the BLOCK-iT Pol II miR RNAi Expression Vector Kit guidelines (Invitrogen). Auts2 miRNA constructs were subcloned into the pCL20c-trL7 ...
-
bioRxiv - Immunology 2020Quote: ... naïve OT-I and OT-II cells were obtained by negative selection using the Dynabeads Untouched Mouse CD4 cell kit (ThermoFisher). Flow cytometry indicated >85% enrichment in all cases ...
-
bioRxiv - Immunology 2023Quote: ... Mouse FM expression of IL1B and KC was measured by RT-qPCR using the SuperScript II kit (Invitrogen; Waltham, MA) and the Kapa SYBR Fast qPCR kit (Sigma-Aldrich ...
-
bioRxiv - Immunology 2024Quote: ... naïve CD4+ T cells were enriched from homozygous TCR-transgenic OT-II (CD45.1/.2) donors using the Dynabeads Untouched™ Mouse CD4 Cells kit (Invitrogen). 1 x 105 OT-II cells were transferred intravenously (i.v. ...
-
bioRxiv - Biophysics 2021Quote: HEK293F cells grown in a 6-well plate were transiently transfected with CFTR constructs labelled with C-terminal eGFP tag using Lipofectamine 3000 (Thermo Fisher) in Opti-MEM (GIBCO ...
-
bioRxiv - Immunology 2021Quote: SPRR genes containing a C-terminal 6xHis tag were cloned into a pFastBac1 vector and heterologously expressed in Sf9 cells (Thermo Fisher). One liter of cells (2.5×106 cells/mL ...
-
bioRxiv - Biochemistry 2019Quote: ... variants of interest were engineered to contain a C-terminal hexahistidine tag to enable purification from conditioned media using HisPur Cobalt Resin (ThermoFisher Scientific). Sample purity was verified by the observation of only a single band on an SDS-PAGE gel following purification.
-
bioRxiv - Immunology 2021Quote: ... containing additional S383C and D985C substitutions (Henderson et al., 2020) with a C-terminal TwinStrep tag into FreeStyle 293-F cells (Thermo Fisher) using polyethyleneimine ...
-
bioRxiv - Cell Biology 2020Quote: The full-length bovine PLCγ1 with an N-terminal His10 tag and a C-terminal SNAP tag was expressed in SF9 cells using the Bac-to-Bac baculovirus expression system (Life Technologies). Cells were harvested by centrifugation and lysed by Dounce homogenizer in 50 mM HEPES (pH 7.4) ...
-
bioRxiv - Plant Biology 2021Quote: ... and pUBC-NKS-GFP/RFP (C-terminal fusion) plant expression vectors (Grefen et al., 2010) by Gateway LR clonase mix (11791-019, Life Technologies). The resultant constructs were transformed into Agrobacterium tumefaciens GV3101 ...
-
bioRxiv - Microbiology 2020Quote: ... The wild type and derivative bteA alleles were cloned downstream from the GAL1 promoter in frame with the C-terminal GFP gene in a low copy centromeric vector pYC2-CT (Invitrogen, USA) carrying the URA3 selection marker ...
-
bioRxiv - Neuroscience 2022Quote: ... 1 μg of plasmid encoding SARS-CoV2 spike (D614) with C-terminal 18 amino acid deletion using Lipofectamine LTX Plus reagent (Invitrogen, USA) as per manufacturer’s protocol ...
-
bioRxiv - Immunology 2022Quote: Expression plasmids encoding His-tagged SARS-CoV-2 RBD (residues 319 to 541 of SARS-CoV-2 S protein) or S protein (with a C-terminal trimerization domain) were cloned into pCEP4 vector and transfected into Expi293F (ThermoFisher Scientific) and the proteins expressed for 7 days at 37°C (Rouet et al. ...
-
bioRxiv - Biochemistry 2021Quote: ... proteins were transferred from the gel onto a polyvinylidene difluoride membrane [36] and detected with the anti-His (C-terminal)-HRP antibody (Thermo Fisher/Invitrogen) according to the manufacturer's instructions ...
-
bioRxiv - Immunology 2021Quote: ... Extracellular fragment of human CD4 comprising domains 1-4 of human CD4 and a C-terminal His6-tag was expressed in Expi293 cells according to the manufactureŕs protocol (Thermo Fisher Scientific). Cell supernatant was harvested by centrifugation 4 days after transfection ...
-
bioRxiv - Microbiology 2021Quote: ... A549 cells were transiently co-transfected with 100 ng in total of an N- or C-terminal LgBiT and SmBiT tagged construct using Lipofectamine™ 3000 Transfection Reagent (ThermoFisher). All possible combinations of the N-terminal and C-terminal-tagged split luciferase protein pairs were tested ...