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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Mouse Procollagen II C Terminal Propeptide PIICP CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... and pENTR-Megator constructs with pHWG (blasticidinR; C-terminal EGFP tag) using Gateway LR Clonase II (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... mouse monoclonal antibody generated against the C-terminal domain of N-cadherin (Invitrogen; 1:500); anti-CRB1 (a kind gift of Dr ...
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bioRxiv - Biochemistry 2020Quote: ... with either a C-terminal (Thermo Scientific) or N-terminal poly-histidine-tag (Genscript ...
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bioRxiv - Molecular Biology 2020Quote: ... vector using Gateway cloning in frame with the C-terminal V5 tag (Gateway LR Clonase II mix; Life Technologies). The Gli2 P1-6A mutant was previously described (15) ...
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bioRxiv - Plant Biology 2023Quote: ... into the plant expression vectors pB7YWG2 (35S promoter; C-terminal YFP) or pB7RWG2 (35S promoter; C-terminal RFP) using Gateway LR clonase (Invitrogen). To generate the chloroplast-targeted YFP and RFP proteins ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-APP C-Terminal (clone CT695; Invitrogen), mouse anti-APP N-terminal (clone 22C11 ...
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bioRxiv - Cancer Biology 2024Quote: ... FOXA1 C-terminal (Thermo Fisher Scientific: PA5-27157); AR (Millipore ...
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bioRxiv - Plant Biology 2019Quote: ... or the stop codon-less full CDS of PNPAR was recombined into the destination vector pGWB651 for C-terminal fusion with GFP or pGWB617 for C-terminal fusion with 4xMYC through Gateway LR reaction (Thermo Scientific) to create p35S:tPNP-A-GFP and p35S:PNPAR-4xMYC ...
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bioRxiv - Microbiology 2022Quote: ... The two pAcgp67-RBD (residues 333–530) plasmid with a C-terminal 8×His tag were transfected into Sf9 cells using Cellfectin II Reagent (Invitrogen) to produce the recombinant baculoviruses ...
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bioRxiv - Plant Biology 2023Quote: ... with a constitutive CaMV 35S promoter and either a YFP or CFP C-terminal tag using the LR Clonase II enzyme mix (Invitrogen). The YFP-tagged construct was used for the generation of stable transgenic Arabidopsis lines of and co-IP experiments ...
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bioRxiv - Developmental Biology 2021Quote: ... the cDNA fragment was subcloned from pDONR221 to pAWH (a GATEWAY Destination vector for C-terminal 3xHA tagging, DGRC#1096) using LR Clonase II Enzyme mix (11791020, Thermo Fisher Scientific), resulting in pAWH-nord ...
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bioRxiv - Plant Biology 2020Quote: ... for C-terminal fusion using LR reactions (Thermo Fisher Scientific). Rice protoplasts were transformed with these vectors as described previously (Wong et al ...
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bioRxiv - Immunology 2021Quote: ... or for intracellular expression using the pYES2/NTC vector (N-terminal Xpress and C-terminal V5 epitope, Invitrogen). The pYD1 system used the yeast internal Apa1/Ap2 system ...
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bioRxiv - Microbiology 2022Quote: ... was synthesized as a custom peptide with N-terminal acetylation and C-terminal amidation (Life Technologies Europe Bv).
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bioRxiv - Plant Biology 2022Quote: ... the construct was recombined with pB7FWG2 destination vector (YFP in C-terminal) using LR ClonaseMixII kit (Invitrogen) and introduced into Arabidopsis (sbt3.3 ...
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bioRxiv - Microbiology 2023Quote: ... a mammalian secretion sequence and a C-terminal C- tag) was transiently expressed using Expi293F™ cells using the Expi293™ Expression System Kit (Thermo Fisher) as recommended ...
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 Beta RBD for crystallization (residues 328-531 of S protein from GenBank NC_045512.2 with N-terminal signal peptide and C-terminal 8xHis-tag) was expressed in Expi293F (Thermo Fisher Scientific) cells in the presence of 10 µM kifunensine at 37°C and 8% CO2 ...
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bioRxiv - Plant Biology 2021Quote: ... or GLK2 was recombined into the destination vector pGWB605 for C-terminal fusion with sGFP or into pGWB617 for C-terminal fusion with 4×Myc through the Gateway LR reaction (Thermo Scientific). For the 35S:GLK1-4×Myc (or sGFP ...
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bioRxiv - Immunology 2021Quote: ... GenBank: QHD43416.1) with an N-terminal IgKappa signal sequence (METDTLLLWVLLLWVPGSTG) and C-terminal TwinStrep- and 3xFLAG-tags (GeneArt, ThermoFisher, Regensburg, Germany) into a pcDNA3.4(+ ...
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bioRxiv - Immunology 2021Quote: ... were cloned into pCEP4 mammalian expression vector containing N-terminal human Ig kappa leader sequence and C-terminal Avi-tag and His-tag (Invitrogen, USA). Expi293-Freestyle cells cultured at 37°C and 8% CO2 in growth medium containing Expi293 Expression Medium at 3×106/mL in 50 mL media were transfected overnight at 37 °C with 50μg of plasmid in 160μL of ExpiFectamine plus 6mL of OptiMEM-I (all from Invitrogen) ...
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bioRxiv - Plant Biology 2022Quote: The full-length coding sequence of the genes were cloned into pSPYNE-35SGW (N-terminal YFP) and/or pSPYCE-35SGW (C-terminal YFP) through gateway cloning (Invitrogen™). BiFC was carried out as described (Gou et al. ...
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bioRxiv - Developmental Biology 2024Quote: HEK293T cells were transferred with C-terminal HA-tagged Kayak and N-terminal GFP-tagged Jun-epi or Kayak using Lipofectamine 2000 (11668030, Thermo Fisher). The GFP-trap agarose beads (ABIN509397 ...
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bioRxiv - Microbiology 2021Quote: ... and one derived from the C-terminal region (SDGGRDEVEKNNGLDVIR, Thermo Fisher, USA) (1:4000 dilution of affinity purified antibody stock of 2.95 mg/ml) ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were labeled with the rabbit anti-tau (C-terminal) antibody (MilliporeSigma) or the mouse anti-phospho-Tau (Thr181) Clone AT270 antibody (Thermo Fisher Scientific) at room temperature at dilution of 1:1000 in 1% BSA for 1 hour and then labeled with Alexa Fluor488 goat anti-mouse IgG secondary antibody at a dilution of 1:500 for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: SARS-CoV-2 RBD WT for SPR binding assays (with N-terminal signal peptide and C-terminal thrombin cleavage site-TwinStrep-8xHis-tag) was expressed in Expi293F (Thermo Fisher Scientific) cells at 37°C and 8% CO2 ...
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bioRxiv - Immunology 2021Quote: ... NC_045512.2 with N-terminal signal peptide and C-terminal thrombin cleavage site-TwinStrep-8xHis-tag) were expressed in Expi293F (Thermo Fisher Scientific) cells at 37°C and 8% CO2 ...
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bioRxiv - Biochemistry 2021Quote: SARS-CoV-2 RBD proteins for SPR binding assays (residues 328-531 of S protein from GenBank NC_045512.2 with N-terminal signal peptide and C-terminal thrombin cleavage site-TwinStrep-8xHis-tag) were expressed in Expi293F (Thermo Fisher Scientific) cells at 37°C and 8% CO2 ...
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bioRxiv - Biochemistry 2021Quote: ... and a C-terminal hexa-histidine tag (GeneArt Gene Synthesis, Thermo Fisher Scientific). The HexaPro expression plasmid was a gift from Jason McLellan (Addgene plasmid #154754 ...
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bioRxiv - Developmental Biology 2022Quote: ... C-terminal HCFC1 (Bethyl labs Cat # 50- 156-0286 purchased through Fisher Scientific) (anti-rabbit ...
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bioRxiv - Cancer Biology 2020Quote: ... and labeled with GeneChip WT Terminal Labeling Kit (Affymetrix) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... Terminal sequences were obtained using 5’ RACE Kit (ThermoFisher) and an anchored oligo-dT primer for the 3’end ...
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bioRxiv - Microbiology 2021Quote: ... [C]-Ahx-QAHAEERLEDIDYVE-acid (Cambridge Research Biochemicals, Billingham, UK) and C-terminal region of FtsZ2: NH2-[C]-SDGGRDEVEKNNGLDVIR-COOH (Thermo Fisher Scientific). H ...
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bioRxiv - Cell Biology 2023Quote: ... and GeneChip WT Terminal labeling Kit and Controls Kit (Thermo Fisher Scientific) following the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2024Quote: ... fixed fibroblasts were incubated with primary antibodies specific to procollagen I (Invitrogen, Waltham, Massachusetts, 1:200), αSMA (sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... RELA with C-terminal HA tag into Gateway donor vector (pCR8/GW/TOPO, Invitrogen). Then LR clonase II reactions were used to shuttle ORFs into pCW-DEST (lentiviral Dox-inducible expression ...
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bioRxiv - Microbiology 2022Quote: ... and a C-terminal isotope-labeled (“heavy” peptide) version was synthesized (Thermo Fisher Scientific).
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bioRxiv - Biophysics 2023Quote: ... The C-terminal cysteine residue was labeled with Alexa 488 C5-maleimide (ThermoFisher Scientific). The labeling was performed in 50 mM phosphate buffer at pH 7 with 100 mM NaCl and 8 M Urea ...
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bioRxiv - Biochemistry 2021Quote: ... mature factor X (FX) protein preceded by the prothrombin signal sequence and propeptide were purchased from GenArt (Thermo Fisher Scientific). N-glycosylation acceptor sites were introduced successfully at modelled surface exposed sites of publicly available crystal structure of FXa (PDB id 1FAX and residues 60 ...
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bioRxiv - Cell Biology 2020Quote: ... for one hour at 25°C and incubated overnight with the rabbit anti-tau (C-terminal) antibody (MilliporeSigma) or the mouse anti-phospho-Tau (Thr181) Clone AT270 antibody (Thermo Fisher Scientific, Waltham, MA, USA) at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... each fused to a C-terminal HA-tag using lipofectamine 3000 transfection reagent (ThermoFisher, L3000015) according to manufacturer instructions ...
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bioRxiv - Bioengineering 2022Quote: ... a C-terminal gp160 trimerization domain and SnoopTagJr) were expressed in suspension Expi293F (Thermo Fisher) and ExpiCHO-S cells respectively and purified as described above for DogCatcher-RBD.
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bioRxiv - Developmental Biology 2022Quote: ... and labelled with the GeneChip WT Terminal Labeling Kit (ThermoFisher Scientific) as per manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... and terminally labeled using the GeneChip WT Terminal Labeling kit (Affymetrix) incorporating biotinylated ribonucleotides into the DNA ...
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bioRxiv - Physiology 2022Quote: ... C-IV-II and C-V-alpha (ThermoFisher Scientific, 45-8199; 1:2500). Secondary antibodies used were horseradish peroxidase–conjugated anti-mouse (Cell Signaling Technology ...
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bioRxiv - Plant Biology 2019Quote: ... or pDEST-24 carrying a C-terminal GST purification tag (Thermo Fisher Scientific, Hampton, NH, USA).
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bioRxiv - Evolutionary Biology 2019Quote: ... The SsPrx03 and SsPrx03m1-TagRFP C-terminal fusion was then subcloned (LR reaction, Gateway Technology, Invitrogen) into pEAQ-HT-DEST1 vector (Sainsbury et al. ...
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bioRxiv - Microbiology 2022Quote: ... The protein with a C-terminal Strep-tag was expressed in Expi293 cells (Thermo Fisher Scientific) and subsequently purified using Strep-Tactin XT Superflow high capacity resin (IBA Lifesciences ...
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bioRxiv - Biochemistry 2020Quote: ... The protein with the C-terminal Fc-fusion was affinity purified with protein-A sepharose (Invitrogen). Samples were eluted with 0.1 M glycine pH 3.0 directly to a neutralizing buffer ...
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bioRxiv - Molecular Biology 2021Quote: Mimivirus L375 appended with a C-terminal 10X histidine tag was generated by GeneArt (Life Technologies) for codon-optimized expression in Escherichia coli ...
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bioRxiv - Microbiology 2020Quote: ... Bound protein was detected via the C-terminal 6XHis Tag using 6XHis Antibody MA1-135 (Invitrogen) followed by three washes in TBS-T and incubation with donkey anti-mouse horseradish peroxidase (HRP)-coupled secondary antibody (Dianova) ...