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Citations for Thermo Fisher :
551 - 600 of 619 citations for Methylene Chloride 13C 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... mRNAs were synthesized by in vitro transcription with 1μl of GTP from the kit added to the mix and lithium chloride precipitation (using the mMESSAGE mMACHINE T7 Ultra kit #AM1345, Ambion). the pCS2+_ancBE4max-SpymacCas9 has been linearized using KpnI restriction enzyme ...
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bioRxiv - Immunology 2021Quote: ... Spleens and lungs were mashed through a 70-μm strainer before red blood cells were lysed using ammonium-chloride-potassium (ACK) lysis (Gibco). Cells were washed with PBS containing 1% FCS and surface receptors were stained using various antibodies ...
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bioRxiv - Neuroscience 2020Quote: ... red blood cells (RBCs) were lysed for 5 min at room temperature using ammonium-chloride-potassium (ACK) buffer (Life Technologies). After centrifugation ...
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bioRxiv - Genomics 2022Quote: ... The undigested pieces of lamina propria were then washed thoroughly with PBS (with calcium and magnesium chloride; Gibco 14080-055) to get rid of all EDTA ...
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bioRxiv - Synthetic Biology 2019Quote: ... of DNA was prepared with specific combinations of plasmids and brought up to a volume of 100 µL using 0.15 M sodium chloride (NaCl, Fisher Scientific). Next ...
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bioRxiv - Developmental Biology 2019Quote: ... In vitro transcription of Tol2 mRNA and lithium chloride precipitation/purification was performed using the SP6 mMessenger Kit (Ambion #AM1340) according to manufacturers guidelines.
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bioRxiv - Plant Biology 2021Quote: ... protoplasts were resuspended in lysis buffer (45mM magnesium chloride, 30mM sodium citrate, 20mM MOPS, 0.5% triton, 2% 4’,6-diamidino-2-phenylindole (DAPI, Thermo Scientific), pH 7 ...
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bioRxiv - Developmental Biology 2020Quote: ... mRNAs were synthetized by in vitro transcription with 1μl of GTP added to the mix and lithium chloride precipitation (using the mMESSAGE mMACHINE sp6 Ultra kit #AM1340, Ambion).
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bioRxiv - Immunology 2021Quote: ... Red blood cells were then lysed by adding 3 ml of ammonium-chloride-potassium (ACK) lysis buffer (Invitrogen, Carlsbad, CA) for 4 min at room temperature ...
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bioRxiv - Immunology 2020Quote: ... Red blood cells were lysed with 1 ml ammonium-chloride-potassium (ACK) lysis buffer (Quality Biological, Gaithersburg, MD, USA) for 1 minute and neutralized with ice-cold DMEM (Gibco). Cells were processed for RNAseq in FACS buffer (2% fetal bovine serum (FBS ...
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bioRxiv - Developmental Biology 2021Quote: ... Each plasmid was linearized with NotI restriction enzyme and mRNAs were synthesized by in vitro transcription with 1 µL of GTP from the kit added to the mix and lithium chloride precipitation (using the mMESSAGE mMACHINE sp6 Ultra kit #AM1340, Ambion).
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bioRxiv - Neuroscience 2023Quote: ... 10 μM gramicidin was added to the internal recording solution to induce chloride-impermeable pore formation along with 25 μM Alexa Fluor 568 hydrazide Na+ salt (Invitrogen) to visualize any potential pore rupture ...
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bioRxiv - Immunology 2022Quote: ... and red blood cells of fresh spleens or bone marrow were lysed in ammonium-chloride-potassium buffer lysing buffer (Gibco) for 3 min ...
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bioRxiv - Cell Biology 2023Quote: ... cells were resuspended in growth media containing octadecyl rhodamine B chloride (R18, ThermoFisher, 1:1000 dilution of 10mg/ml stock), centrifuged ...
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bioRxiv - Immunology 2023Quote: ... Cells were centrifuged at 1500 rpm for five minutes and then resuspended in 5mL of Ammonium-Chloride-Potassium (ACK) lysis buffer (Gibco) on ice for 10 minutes ...
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bioRxiv - Microbiology 2024Quote: ... red blood cells were lysed by addition of 1 ml ammonium chloride-potassium carbonate (ACK) lysis buffer (Life Technologies, A1049201). ACK was neutralized by the addition of cRPMI ...
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bioRxiv - Cancer Biology 2024Quote: ... The pellet was resuspended in 200–500 µl of ACK (ammonium-chloride-potassium) RBC lysis buffer (Thermo Fisher Scientific, A1049201) and incubated for 1 min on ice ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Lot 183859), ammonium formate (99%, AC401152500), acetonitrile (ACN; Optima®, Lot 184819), and formic acid (99.5%, A117-50) from Fisher Scientific (Waltham, MA) was used for extractions and instrument solvent gradient ...
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bioRxiv - Neuroscience 2021Quote: Zebrafish larvae were incubated for 2 hours with 1:100 LysoTracker™ Green DND-26 or LysoTracker™ Red DND-99 (ThermoFisher Scientific) in E3 medium (final concentration of 10 µM) ...
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bioRxiv - Molecular Biology 2021Quote: ... containing varying concentrations of the corresponding crowding agent: 0/5/10/305515% w/v of serine (L-Serine, 99%, ACROS Organics™, AC132660250), 0/5/10/15% w/v of Polyethylene glycol 3350 (PEG ...
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bioRxiv - Neuroscience 2023Quote: Fly brains expressing Atg8-mcherry were dissected in Schneinder’s media and stained for 3 minutes with lysotracker (LysoTracker Red DND-99; Thermo Fisher Scientific; 1:2000), rinsed in Schneinder’s media and then directly visualised with a Zeiss Airyscan 880 ...
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bioRxiv - Biochemistry 2023Quote: ... blotted from both sides for 3 seconds at 4 ℃ under 99% humidity and plunge frozen in liquid ethane using Vitrobot Mark IV (Thermo Fisher Scientific, Waltham, MA). Images were recorded using a Titan Krios G4 microscope at the University of Tokyo (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: For the staining of lysosomes in living cells Lysotracker Red DND-99 in a final concentration of 50 nM (Thermo Fisher cat. no. L7528) was used according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... Annealing was done by heating to 99 degrees and slowly decreasing the temperature 1°C /min in a Veriti™ 96-Well Thermal Cycler (Applied Biosystems, Cat. 4375786). After annealing ...
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bioRxiv - Molecular Biology 2020Quote: Single- and double-stranded oligonucleotide substrates were diluted in TBST with 0.1% BSA and 3 mM magnesium chloride to 1 μM final concentrations Previously mentioned enzymes and RNase A (EN0531, Thermo Fisher) were used at a 1:200 final dilution and incubated with indicated nucleic acid substrates for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... Enzymatic treatments were done in staining buffer supplemented with 3 mM magnesium chloride with 1:200 dilutions of RNase T1 (EN0541, Thermo Fisher), RNase III (ShortCut RNase III ...
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bioRxiv - Cell Biology 2020Quote: ... solutions of E3 + Tricaine and E3 + Tricaine + 135 mM sodium chloride were prepared and supplemented with 10µM of CoroNa Green (Invitrogen Cat#C36675). A wildtype larva was mounted in the device as described above and confocal image stacks were collected over time as the two CoroNa Green containing media were flowed into either chamber of the device ...
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bioRxiv - Microbiology 2020Quote: ... BMDM with bacteria were incubated for 30 min at 37°C/5% CO2 and then washed three times with warm DPBS++ (+Calcium and +Magnesium Chloride – Gibco 14040). One mL fresh DMEM-10 with Gentamicin (50 μg/mL ...
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bioRxiv - Neuroscience 2022Quote: ... The spleen was mashed with the plunger of a syringe and treated with ammonium-chloride-potassium (ACK) lysis buffer (Gibco™) to remove erythrocytes ...
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bioRxiv - Immunology 2022Quote: ... membranes were washed three times with PBS-T and then incubated with appropriate secondary antibody conjugated with alkaline phosphatase that provides a visual color change upon addition of the chromogenic substrate (mixture of BCIP (5-bromo-4chloro-3-indolyl phosphate-catalog# 34040) and NBT (nitro-blue tetrazolium chloride, catalog# 34035 from Thermo Scientific)).
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bioRxiv - Neuroscience 2022Quote: ... 1xPBS was replaced with direct stochastic optical reconstruction microscopy (dSTORM) photo-switching buffer consisting of 50 mM Tris pH 8 solution supplemented with 10 mM sodium chloride (ThermoFisher Scientific), 10% glucose (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2019Quote: ... or dequalinium chloride (ranging 1 to 500 μg/ml) in Flat white 96-well plates with optical bottom (Thermo Scientific Nunc), at a final OD600nm of 0.01 ...
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bioRxiv - Immunology 2021Quote: ... Lymph nodes and spleen were mechanically pushed through 70 μm nylon mesh cell filters followed by RBC lysis with ACK (ammonium-chloride-potassium) lysing buffer (ThermoFisher Scientific) solution ...
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bioRxiv - Microbiology 2019Quote: Porewater sulfate and chloride concentrations were measured in a Dionex ICS-5000 reagent-free ion chromatography system (Thermo Scientific, CA, USA) equipped with an anion-exchange column (Dionex IonPac AS22 ...
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bioRxiv - Cell Biology 2021Quote: ... Single cell-suspensions of lymphocytes were achieved by sterile dissociation of whole spleens with syringe plunger through cell strainer and by lysing red blood cells with the ACK (Ammonium-Chloride-Potassium) lysing buffer (Life Technologies). Isolated splenocytes were pooled together and placed in 24-well plates at 106 cells per ml concentration in 1.5 ml and activated with anti-CD3 mAb (145-2C11 clone ...
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bioRxiv - Immunology 2020Quote: ... in cap-locked adaptors and cells were lysed in Ammonium Chloride Potassium (ACK) lysing buffer at room temperature for 5 minutes (Gibco# A1049201) washed in 2% FBS ...
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bioRxiv - Microbiology 2021Quote: ... 1 mL aliquots of the BLEB enrichments were serially diluted to 10-3 in phosphate buffered saline (PBS; 1M; potassium chloride [Fischer Chemical], potassium phosphate [Acros Organics] ...
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bioRxiv - Immunology 2021Quote: ... The final wash was followed by the addition of Nitro-blue Tetrazolium Chloride/5-bromo-4-chloro 3 ‘indolyl phosphate p-toludine salt (NBT/BCIP chromagen) substrate solution (Thermo Scientific) for 7 min ...
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bioRxiv - Immunology 2022Quote: ... in 50 μl sterile Dulbecco’s Phosphate Buffered Saline without either Calcium or Magnesium Chloride (DPBS, #14190-144; ThermoFisher Scientific, Waltham, USA) intratracheally once a week for three weeks straight ...
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bioRxiv - Molecular Biology 2022Quote: ... The RNA was then incubated with 5M sodium chloride and isopropanol and incubated with Dynabeads M-270 Streptavidin (Thermo Fisher, 65305) for 1 hour at room temperature ...
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bioRxiv - Immunology 2022Quote: Spleens were mechanically dissociated to single cells followed by RBC lysis in 1 ml of Tris-ammonium chloride (ACK) lysis buffer (Life Technologies) for 2 min at room temperature (rt) ...
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bioRxiv - Neuroscience 2022Quote: ... Red blood cells were removed by incubating the blood samples with 2mL Ammonium-Chloride-Potassium Lysing Buffer (Thermo Fisher Scientific #A1049201) for 3 to 5minutes at RT ...
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bioRxiv - Microbiology 2023Quote: ... 2TY media components (tryptone, yeast extract, sodium chloride) and cation-adjusted Mueller Hinton Broth (CAMHB; Becton Dickinson) were purchased from Fisher Scientific.
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bioRxiv - Genomics 2023Quote: Whole ovaries were isolated using aseptic technique and placed in ice cold and sterile 1X Phosphate Buffered Saline (1X PBS, without calcium and magnesium chloride; Gibco 10010023). Blood and other contamination were carefully removed by washing the tissues with fresh 1X PBS ...
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bioRxiv - Immunology 2023Quote: ... Single-cell suspensions of spleens were prepared using 70-μm cell strainers and red blood cells lysed by incubation in Ammonium-Chloride-Potassium (ACK) Lysing Buffer (Life Technologies). Single-cell suspensions of lungs were prepared by digestion with 0.25mg/ml Collagenase IV and 15 μg/ml DNase for 1 hour at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... chloride hemipentahydrate (Cd2+), lead (II) nitrate (Pb2+), mercury (II) chloride (Hg2+), and hydrogen peroxide (H2O2, 30% in water) were purchased from Fisher Scientific. Buthionine sulfoximine (BSO) ...
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bioRxiv - Neuroscience 2023Quote: ... and whole media fractions were mixed with the fluorescent membrane dye Octadecyl Rhodamine B Chloride R18 (0.33 ng/ul, Life Technologies, O246) and incubated for 10 min at room temperature ...
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bioRxiv - Genetics 2023Quote: ... tissue samples were incubated overnight in lysis buffer (50 mM Tris-HCl pH 8.0, 100 mM EDTA, 100 mM sodium chloride, and 1% SDS; Thermo Fisher Scientific) at 55°C and 300 rpm ...
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bioRxiv - Microbiology 2023Quote: ... and ATTO647N (Atto-Tec) were dissolved in DMSO (10 and 5 mg.mL-1, respectively) while octadecyl rhodamine B chloride (R18, Thermo Fisher Scientific) was dissolved in ethanol (10 mM).
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bioRxiv - Cancer Biology 2023Quote: ... (4) 30 min incubation in ammonium chloride (NH4Cl) and (5) 4 min incubation in Tissue Autofluorescence Quenching Kit (ReadyProbes, ThermoFisher Scientific).Secondary antibodies were used as follows ...