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Citations for Thermo Fisher :
501 - 550 of 619 citations for Methylene Chloride 13C 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... at 100 µg/mL in complete DMEM medium for 4 h and treated with LysoTracker Red DND-99 (Invitrogen #L7528) for 15 min and subsequently imaged on a Nikon CSU-W1 SoRa microscope ...
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bioRxiv - Developmental Biology 2023Quote: Acidic organelles in the oocytes were visualized using LysoTracker Red DND-99 and LysoSensor Green DND-189 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... The conductivities of each alkali chloride were recorded using an Accumet AB200 pH/Conductivity Benchtop Meter (Fisher Scientific). The results were as follows ...
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bioRxiv - Bioengineering 2022Quote: ... filtered trypan blue solution (0.4% trypan blue in 0.81% sodium chloride and 0.06% dibasic potassium phosphate; Life Technologies) was prepared and incubated for 2 min at room temperature for viable/non-viable cell imaging under the phase contrast microscope ...
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bioRxiv - Biochemistry 2019Quote: ... was incubated overnight in 0.1 M sodium formate / 0.15M sodium chloride/ 0.1% reduced Triton X-100 pH 3.5 with dialysis against the same buffer (20kDa MWCO, ThermoFisher) to convert TPP1 to a mature/active form and to remove the cleaved pro-domain ...
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bioRxiv - Bioengineering 2020Quote: ... EVs are either non-specifically labeled using the fluorescent membrane dye Octadecyl Rhodamine B Chloride R18 (Life Technologies, catalog# O246 ...
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bioRxiv - Microbiology 2020Quote: ... diluted in Earle’s balanced salt solution supplemented with 200 mg/ml Calcium Chloride (Gibco, Life Technologies, Renfrew, UK), at 37°C with continual gentle mixing of the tube ...
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bioRxiv - Microbiology 2020Quote: ... diluted in Earle’s balanced salt solution supplemented with 200 mg/ml Calcium Chloride (Gibco, Life Technologies, Renfrew, UK), at 37°C with continual gentle mixing of the tube ...
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bioRxiv - Microbiology 2020Quote: ... in ice-cold RIPA buffer (150 mM sodium chloride, 1.0% Triton X-100, 0.5% sodium deoxycholate, 0.1% SDS, 50 mM Tris, pH 8.0; Thermo Fisher Scientific), supplemented with complete Protease Inhibitor Cocktail (Sigma Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed once with Krebs-Ringer’s buffer containing 1.8 mM calcium chloride and loaded with Fluo-4 AM (1 μg/μl; ThermoFisher) plus 10 μl 0.2 % pluronic acid for 30 min at 37 °C ...
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bioRxiv - Physiology 2023Quote: ... The pellets were then resuspended and incubated with ammonium-chloride-potassium (ACK) lysing buffer (Thermo Fisher Scientific, USA) for 3 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... For nuclear staining 0.5µg/µL of DAPI (4’, 6-diamidino-2-phenylindole, dihydro-chloride, Invitrogen. Cat. No.: D3571) was added along with secondary antibody ...
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bioRxiv - Microbiology 2024Quote: ... paramagnetic beads were washed three times with 1X wash buffer (50 mM Tris-HCl pH 7.5 – 150 mM sodium chloride – 0.05% Tween-20 – 1 mM dithiothreitol) using DynaMag-2 magnetic stand (Invitrogen). Mpro-Avi (6 ug ...
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bioRxiv - Cell Biology 2019Quote: ... To monitor for bacterial incorporation into phagolysosomes OCs were further incubated with the acidotrophic probe Lysotracker Red® DND-99 (100 nm, Invitrogen) for 30 min prior to fixation ...
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bioRxiv - Biophysics 2021Quote: ... and biotin-PEG-SVA at a ratio of 99:1 (w/w) (Laysan Bio) in 0.1 M sodium bicarbonate (Thermo Fisher Scientific) for 3 h.10 Excess PEG was rinsed with water ...
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bioRxiv - Genetics 2021Quote: ... worms grown at 25°C were incubated 48 hours prior to imaging with 2 μM LysoTracker Red DND-99 (Life Technologies). Worms were imaged as day 4 adults as described above ...
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bioRxiv - Cell Biology 2020Quote: ... for 10 minutes in live-cell imaging medium and lysosomes were visualized by staining cells with 50nM Lysotracker Red DND-99 (Thermo Fisher) for 30 minutes in live-cell imaging medium according to manufacturer’s description ...
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... each well was gently rinsed once with RPMI and then starved for 50 min in 2 mL of RPMI (without amino acids, glucose or FBS) with 20 μM Lysotracker Red DND-99 (Invitrogen, L7528). After 50 min of starvation ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell differentiation was induced when cells reached around 90% confluence by replacing the proliferation medium with 99% (v/v) high glucose DMEM (Gibco, UK), 1% (v/v ...
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bioRxiv - Cell Biology 2020Quote: ... Live glands requiring staining were treated for 5 min in a 1:100 dilution of LysoTracker Red DND-99 (Life Technologies) in ice-cold PBS.
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bioRxiv - Microbiology 2021Quote: ... CAS number: 328–42–7), 2-Ketoglutaric acid, disodium salt, dehydrate (>99%, CAS number: 305–72–6) were purchased from Acros Organics, Belgium ...
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bioRxiv - Immunology 2022Quote: Acidic vesicles were visualised by incubating RAW 264.7 cells in medium enriched with LysoTracker™ Red DND-99 (1:2500) (Invitrogen, L7528) for at least 1 hour prior to fixation at 37°C [35] ...
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bioRxiv - Cell Biology 2022Quote: Lysosomal mass was estimated by incubating cells under various genetic perturbations (7 days after sgRNA transduction) with LysoTracker Red DND-99 at 100 nM (Invitrogen; L7528). After incubation at 37ºC for 1 hour ...
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bioRxiv - Physiology 2023Quote: ... the gut of control or rapamycin-treated flies were dissected out in 4°C chilled PBS and then stained in a 10 μM Lysotracker Red:1μg/ml Hoechst solution (LysoTracker™ Red DND-99, Hoechst 33342 Solution, Thermofisher Scientific) for 3 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... LMD samples were incubated at 99 °C for 30 min followed by 50 °C for 10 min with SMART trypsin (ThermoFisher Scientific). SMART trypsin was added at a ratio of 1 μg per 30 mm2 tissue ...
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bioRxiv - Bioengineering 2023Quote: ... Samples were incubated for 5 min at 99°C before loading on 4-12 % SDS-PAGE with MOPS as running buffer (Thermo-Fisher Life Technologies). BenchMark protein ladder was used as marker (Invitrogen) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Lot 134874), and 4-(2-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, purity ≥ 99.%, CAT #BP310-1, Lot 052975) were from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Neuroscience 2024Quote: For the lysosomal functionality assay fibroblast cells were incubated with CM containing 100 nM LysoTracker™ Red DND-99 (Thermo Fisher) for 5h ...
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bioRxiv - Bioengineering 2021Quote: ... The virus was then dialyzed with 1× phosphate buffered saline (pH 7.2) supplemented with 50 mM sodium chloride and 0.0001% Pluronic F68 (Thermo Fisher) using 50 kDA filters (Millipore) ...
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bioRxiv - Microbiology 2021Quote: ... was accomplished by reacting the respective oligosaccharide glycamines with Lissamine rhodamine B sulfonyl chloride (LRSC) (Acros Organics, Belgium, USA) as described previously (64) ...
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bioRxiv - Developmental Biology 2020Quote: ... followed by Poly(A) tailing procedure and lithium chloride precipitation (using the mMESSAGE mMACHINE T7 Ultra kit #AM1345, Ambion). pCMV-ancBE4max (pCMV_AncBE4max was a gift from David Liu (Addgene plasmid # 112094 ...
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bioRxiv - Microbiology 2020Quote: Approximately 5 μg of BAC DNA was linearized with NotI and full-length RNA was obtained by in vitro transcription with T7 RNA polymerase followed by lithium chloride precipitation according to the manufacturer’s protocol (mMessage-mMachine T7 Kit; Ambion). 5 μg of RNA was electroporated into 5×106 BHK-21 cells using the Amaxa nucleofector 2b (program A-031 ...
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bioRxiv - Immunology 2022Quote: ... for 5 minutes and pellets were resuspended in Ammonium-Chloride-Potassium (ACK) lysing buffer (Thermo Fisher Scientific, Catalog # A1049201) to lyse RBC (erythrocytes removal performed at R.T ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were fixed and stained with 200uL of 1 mg/mL solution of Nitro Blue Tetrazolium Chloride (Invitrogen, N6495). Six biological replicates were performed ...
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bioRxiv - Bioengineering 2023Quote: ... HA-tetrazine was synthesized as described previously using 79 kDa HA with molar equivalents of 1:1:0.25 of HA-repeat units to 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM) (Thermo Fisher Scientific) to tetrazine-amine (Chem-Impex).[16] 1H NMR shifts of pendant tetrazine groups at δ8.5 (2H ...
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bioRxiv - Neuroscience 2024Quote: ... Virus was diluted in sterile 0.9% w/v sodium chloride in low protein binding collection tubes (Life Technologies Ltd) and stored at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were washed gradually into 2X SSC buffer (0.3 M sodium chloride, 30 mM sodium citrate or AM9763, Invitrogen), and then gradually from SSC to PBT ...
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bioRxiv - Microbiology 2023Quote: ... Red blood cells (RBCs) were then lysed by resuspending cells in ammonium-chloride-potassium (ACK) lysis buffer (Thermo Fisher) for 2 min ...
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bioRxiv - Cell Biology 2020Quote: ... washed with organoid media and then incubated with 100 cell pellet volumes of organoid media with 10 nM LysoTracker Red DND-99 (Thermo Fisher L7528) at 37C for 30 minutes ...
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bioRxiv - Immunology 2019Quote: ... was carried out in 10 mM Tris-HCl – 1 mM EDTA buffer (pH 9) in +99°C for 20 min (PT Module; Thermo Fisher Scientific). Tissue peroxide quenching with 0.9% H2O2 for 15 min was followed by protein blocking with 10% normal goat serum (TBS-NGS ...
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bioRxiv - Immunology 2022Quote: ... Grids were blotted from the backside for 10 seconds at 4℃ under 99% humidity and plunge frozen in liquid ethane using Vitrobot Mark IV (Thermo Fisher Scientific). We removed one of the blotting arms for one-side blotting because Birbeck granules would be absorbed onto the filter paper ...
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bioRxiv - Biophysics 2022Quote: Lysosomes were stained by loading the cells with LysoTracker™ Red DND-99 (1 mM, ref. L7528) at a final concentration of 50 nM purchased from Life Technologies | Thermo Fisher Scientific (Invitrogen) ...
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bioRxiv - Cell Biology 2019Quote: ... the salivary glands were incubated 30 min at RT in Grace’s medium containing 150 nM LysoTracker® Red DND-99 (Molecular Probes L7528), and immediately imaged after incubation ...
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bioRxiv - Immunology 2021Quote: ... the plate was again washed six times and 100 μL/well substrate (200 μL 40 mg/mL o-Phenylenediamine dihydrochloride 99+% (OPD, Acros Organics BVBA) + 2 μL H2O2 (Merck ...
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bioRxiv - Bioengineering 2023Quote: ... All samples were heated at 99 °C for 5 min and separated using Novex Precast 4–20% gels (Invitrogen, Waltham, MA, #WXP42020BOX) in SDS-PAGE ...
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bioRxiv - Bioengineering 2023Quote: ... Dodecane overlay cultivations were performed under the same conditions as controls with no extraction aid but with the addition of 20% of the cultivation volume of sterile dodecane at 99% purity (Acros Organics, UK). All microplate cultivations were made in triplicates to increase the statistical robustness of the results ...
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bioRxiv - Genomics 2023Quote: Adults were relaxed by incubating for 3–16 hours at 4 °C in about 400 mL ASW with approximately 5 g of 99% L-menthol crystals (Thermo Scientific A10474) (3 adults per 400 mL volume) ...
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bioRxiv - Cell Biology 2023Quote: BMDMs were seeded in 96 well plates (6055302, Revvity) and stained with 25 nM LysoTracker DND-99 (LTR) (L7528, Thermo Fisher Scientific) for 30 minutes ...
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bioRxiv - Systems Biology 2023Quote: ... and 99%) were used to dehydrate the slides and a coverslip was mounted with Diamond anti-fade mounting medium (Invitrogen, cat.no. P36961).
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bioRxiv - Microbiology 2020Quote: ... Sections were then incubated in a 48 °C prewarmed saline-sodium citrate wash buffer (30 mM sodium citrate, 300 mM sodium chloride, pH7.4, Invitrogen, France) for 20 minutes ...