Labshake search
Citations for Thermo Fisher :
551 - 600 of 8852 citations for M CSF Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: HEK293 cells were maintained in DMEM/F-12 (Gibco, cat no. 11320033) supplemented with 1% Penicillin-streptomycin (Gibco ...
-
bioRxiv - Biophysics 2023Quote: HEK293 cells were transfected with ICD constructs using Lipofectamine 2000 (Thermo Fisher) and were selected with G418 ...
-
bioRxiv - Cancer Biology 2024Quote: ... HEK293 cells were seeded overnight and transfected with Lipofectamine 3000 (Thermo Fisher) according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2023Quote: HEK293-6E suspension cells were cultured in FreeStyle F17 expression medium (Gibco) supplemented with 4 mM L-glutamine (Gibco) ...
-
bioRxiv - Biochemistry 2024Quote: HEK293 cells (ATCC) were grown in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) with 10% fetal bovine serum (FBS ...
-
bioRxiv - Bioengineering 2024Quote: HEK293-F suspension cells were cultured in FreeStyle 293 Expression Medium (Gibco) in shake flasks at 135 rpm ...
-
bioRxiv - Biochemistry 2024Quote: HEK293 Freestyle suspension cells were cultured in HEK Freestyle Media (Invitrogen, 12338018) grown at 37° C in a humidified shaking platform incubator with 10% CO2 ...
-
bioRxiv - Molecular Biology 2021Quote: ... fluorescence-labeled HIV-1JR-FL Env(-) carrying the (His)6 epitope tag was incubated with biotin-conjugated anti-(His)6 tag antibody (HIS.H8, Invitrogen) at 4° for two hours.
-
bioRxiv - Cell Biology 2019Quote: ... The plasmids encoding for His-SEPT2 or His-SEPT2-mCherry and SEPT6/7-strep were co-transformed into E.coli BL21 (DE3) (Invitrogen). Bacterial cultures were grown to OD600 of 2-3 and induced with 1 mM IPTG for 1h at 37°C (His-SEPT2 ...
-
bioRxiv - Immunology 2021Quote: ... RBD-His and Spike-his containing supernatants were batch purified using the HisPur™ Ni-NTA Resin (ThermoFisher Scientific). Supernatants were incubated with 6 ml of resin for 1h at RT ...
-
bioRxiv - Developmental Biology 2021Quote: ... containing 500 μm disk patterns were coated with 10 μg/ml recombinant human laminin 521 (BioLamina) diluted in pre-warmed DPBS (Thermo Fisher Scientific) for 3 hours at 37°C ...
-
bioRxiv - Cell Biology 2021Quote: ... Phages displaying human Fabs were enriched after three rounds of biopanning on biotinylated recombinant human ACE2 immobilised to streptavidin Dynabeads (Dynal M-280, Invitrogen, cat # 112.06D). After the third round of panning ...
-
bioRxiv - Physiology 2023Quote: ... 0.5-1 µg of total RNA was isolated from human islets and reverse transcribed using an M-MLV RT kit (Invitrogen, MA, USA). TaqMan primers (Applied Biosystems ...
-
bioRxiv - Biochemistry 2023Quote: ApoE was immunoprecipitated from matched participant samples of 20 μL CSF and 2 μL plasma via a monoclonal antibody to human apoE (HJ15.3) (provided by the Holtzman Lab) conjugated to M-270 Epoxy Dynabeads (Life Technologies/Invitrogen, Carlsbad, CA). The CSF samples were prepared by combining 20 μL of neat CSF with 100 μL of the apoE3*/apoE4* astrocyte media internal standard ...
-
bioRxiv - Cell Biology 2024Quote: Knockdown of RORα in human cells was performed using the siGenome SMARTpool Human RORA (#6095) siRNA from the Horizon Discovery “Dharmacon” portfolio (#M-003440-01-005) and Lipofectamine 2000 (Invitrogen, #11668-030). The siRNA stock was diluted to 20 µM in RNase-free water following the product protocols ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... 0.1 M DTT and 200 U of M-MLV reverse transcriptase (Invitrogen).
-
bioRxiv - Biochemistry 2020Quote: ... 17.5ml M-199 (Invitrogen), 10ml Horse serum ...
-
bioRxiv - Microbiology 2020Quote: ... using M-MLV (Invitrogen) or Superscript IV (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2022Quote: ... 0,018 M HEPES (Invitrogen) and 0.2 M NaOH to adjust pH to 7,4 ...
-
bioRxiv - Neuroscience 2021Quote: ... 0.15 M NaCl (ThermoFisher), 25 mM EDTA (Sigma ...
-
bioRxiv - Developmental Biology 2020Quote: ... 0.01 M DTT (Invitrogen) and 150 U SuperScript® II Reverse Transcriptase (Invitrogen ...
-
bioRxiv - Molecular Biology 2022Quote: ... M-MLV (Thermo Fisher), AMV (New England Biolabs) ...
-
bioRxiv - Systems Biology 2023Quote: ... 2 M thiourea (Invitrogen), and 20mM HEPES ...
-
bioRxiv - Cell Biology 2024Quote: ... 1 M HEPES (Gibco) and 1 % (v/v ...
-
bioRxiv - Immunology 2023Quote: ... PBMCs were further stimulated with GM-CSF (2000 IU/mL, Gibco, MT, USA) and IL-4 (1000 IU/mL ...
-
bioRxiv - Cancer Biology 2023Quote: ... PBMCs were further stimulated with GM-CSF (2000 IU/mL, Gibco, MT, USA) and IL-4 (1000 IU/mL ...
-
bioRxiv - Cell Biology 2020Quote: ... Stable transformants of ZP161 expressing rat Pex14 variants tagged with N-terminal hexahistidine (His-Pex14) were isolated by transfection of pcDNAZeo-D/His-RnPEX14 variants (see below) followed by selection with Zeocin (Invitrogen), as described (Okumoto et al. ...
-
Lactic Acid Containing Polymers Produced in Engineered Sinorhizobium meliloti and Pseudomonas putidabioRxiv - Microbiology 2019Quote: ... the gel was used for His-tag staining following the InVision(tm) His-tag In-Gel Stain protocol provided by Invitrogen.
-
bioRxiv - Immunology 2022Quote: ... pAc5.1A/V5-His-CBF and pAc5.1A/V5-His-EF-1α were transfected into S2 cells using Cellfectin II Reagent (Invitrogen, USA), respectively ...
-
bioRxiv - Cell Biology 2020Quote: ... His-tags were removed with AcTEV protease (Invitrogen) overnight and proteins further purified with a HiTrap SP HP cation exchange column (GE Healthcare).
-
bioRxiv - Molecular Biology 2020Quote: A pcDNA3.1/V5-His TOPO expression vector (Invitrogen) encoding the protein-coding sequence of human SM (NM_003129.4 ...
-
bioRxiv - Microbiology 2019Quote: ... mouse anti-His antibody (Thermo Fisher, Waltham, MA) was diluted 1:2000 in 1x iBind solution ...
-
bioRxiv - Biochemistry 2020Quote: ... into pT7-N-His-GST (Thermo Fisher Scientific) or pET28a (EMD Millipore) ...
-
bioRxiv - Biophysics 2021Quote: ... supplemented with 10% HI-FBS (16140-071, Gibco), 100 U/mL Penicillin-Streptomycin (15140122 ...
-
bioRxiv - Biochemistry 2021Quote: ... then cloned into pIB/V5-His vector (Invitrogen), fused with V5 epitope at the C-terminus ...
-
bioRxiv - Biophysics 2020Quote: ... 10 % v/v Hi-Di formamide (Thermo Scientific), supplemented with 2 mM vanadyl ribonucleoside complex) ...
-
bioRxiv - Microbiology 2022Quote: ... and mouse anti His Alexa Fluor 555 (Invitrogen catalogue number MA1-135-A555 ...
-
bioRxiv - Biochemistry 2021Quote: ... 10 % v/v Hi-Di formamide (Thermo Scientific), supplemented with 2 mM vanadyl ribonucleoside complex) ...
-
bioRxiv - Cell Biology 2019Quote: ... p38β (MAPK11, His-tagged, Thermo Fisher Scientific, #PV3679), p38γ (MAPK12 ...
-
bioRxiv - Cell Biology 2019Quote: ... p38γ (MAPK12, His-tagged Thermo Fisher Scientific, #PV3654), p38d (MAPK13 ...
-
bioRxiv - Cell Biology 2019Quote: ... p38d (MAPK13, His-tagged, Thermo Fisher Scientific, #PV3656) and hPI31 (UBPBio ...
-
bioRxiv - Cancer Biology 2020Quote: ... in 1X Hi-Fi PCR buffer (Invitrogen #52045) supplemented with 2 uL of 50 mM MgSO4 and dNTPs (Roche#11-581-295-001 ...
-
bioRxiv - Genetics 2021Quote: ... and Hi - Di™ Formamide (Applied Biosystems, USA). The DNA (PCR products ...
-
bioRxiv - Microbiology 2020Quote: ... MprA was detected using Anti-His antibody (Invitrogen) and anti-mouse HRP-labelled secondary antibody (Santa Cruz) ...
-
bioRxiv - Immunology 2022Quote: ... 10% hi-FBS and 1% penicillin/streptomycin (ThermoFisher) in absence or presence of APRIL (Recombinant Human APRIL/TNFSF13 Protein ...
-
bioRxiv - Microbiology 2022Quote: ... DMEM 2% Hi-FBS 0.5% Ultrapure agarose (Invitrogen) was used to overlay the wells ...
-
bioRxiv - Immunology 2023Quote: ... supplemented with 10% (v/v) HI-FBS (Gibco), 10 U/mL penicillin and 100 U/mL streptomycin (Corning) ...
-
bioRxiv - Cell Biology 2023Quote: ... mouse anti-His (Invitrogen, #MA1-21315, 1:1000), rabbit anti-GST (Merck,#ZRB1223,1:1000) ...
-
bioRxiv - Microbiology 2023Quote: ... anti-6x-His mouse monoclonal (Invitrogen 37-2900), anti-CD99 mouse monoclonal (Invitrogen MA5-12954) ...
-
bioRxiv - Immunology 2023Quote: ... 6x His Tagged monoclonal antibody (Thermo Fisher Scientific) was also plated as an experimental control ...