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Citations for Thermo Fisher :
451 - 500 of 8852 citations for M CSF Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... HEK293S GnTI-cells (ATCC) were cultured in Freestyle 293 medium (GIBCO) supplemented with 2% (v/v ...
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bioRxiv - Neuroscience 2023Quote: ... The siRNAs were transfected into HEK293 cells using Lipofectamine 2000 (Invitrogen). The effect of knockdown was validated by western blot.
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bioRxiv - Biochemistry 2023Quote: ... HEK293-F cells were grown in FreeStyle 293 expression medium (Gibco) (20 ...
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bioRxiv - Biochemistry 2023Quote: HEK293-F cells were grown in FreeStyle 293 Expression Medium (Gibco) at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: HEK293 cells were grown in Dulbecco’s modified Eagle medium (DMEM; Gibco) containing 10% (v/v ...
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bioRxiv - Physiology 2023Quote: HEK293 cells were cultured in Dulbecco’s modified Eagle’s medium (Gibco, France) supplemented with 10% fetal calf serum (Eurobio ...
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bioRxiv - Evolutionary Biology 2023Quote: ... HEK293 cells were washed with Opti-MEM Reduced Serum Media (Gibco) and incubated with transfection mix containing 1 µg total of the indicated plasmid DNA and 3 µL Lipofectamine 3000 Transfection Reagent (Invitrogen ...
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bioRxiv - Physiology 2023Quote: ... HEK293 cells were washed with Opti-MEM Reduced Serum Media (Gibco) and transfected using lipofectamine 2000 (Invitrogen/Life Technologies Cat #11668019 ...
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bioRxiv - Biochemistry 2023Quote: ... After transfection the Flp-In TRex HEK293 (Flp-In-293, Invitrogen) cells were selected in the presence of Hygromycin B (100 µg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 and C166 cells were maintained in DMEM (high glucose, Gibco) supplemented with 10% FBS (Giboco) ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293-FT (RRID:CVCL_6911) were culture similarly but in DMEM medium (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: HEK293-T cells were cultured in DMEM medium (#31966-021, Gibco) supplemented with 10% fetal bovine serum (#10270-106 ...
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bioRxiv - Neuroscience 2023Quote: HEK293 cells were grown in Dulbecco’s Modified Eagle Medium (DMEM, ThermoFisher) containing 10% fetal calf serum and 0.5% penicillin/streptomycin ...
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bioRxiv - Microbiology 2020Quote: ... upon which 1 μg of yeast RNA and 10× structure buffer (0.1 M Tris at pH 7, 1 M KCl, 0.1 M MgCl2; Ambion) were added ...
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bioRxiv - Biochemistry 2021Quote: ... Then pH standard buffers (pH ~6.5 and ~7.5 (0.025 M HEPES, 0.105 M KCl, 0.001 M MgCl2)) were prepared with 10 μM nigericin (Invitrogen, N1495) and added to cells to equilibrate pHe and pHi as previously described.28 For each cell ...
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bioRxiv - Cell Biology 2021Quote: Human PCNT siRNA (Smart Pool) (M-012172-01-0005; Dharmacon) was transfected into cells with Lipofectamine RNAi MAX (13778100; ThermoFisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... His-tagged GOT2 protein was immunoprecipitated using the His-tag isolation and pull-down Dynabeads system (Thermo Fisher) using the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and further ligated into pcDNA 3.1 V5/His TOPO vector using pcDNA 3.1 V5/His TOPO TA cloning kit (Invitrogen). The ACRD etoll was then transferred to pGADT7-AD vector using Nde1 and BamH1 sites ...
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bioRxiv - Cell Biology 2022Quote: ... The His SNX32ΔC protein bound to the lipids was detected by anti-His antibody [1:10,000 (Thermo Scientific)]
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bioRxiv - Molecular Biology 2023Quote: ... ceSMSγ and ceSMSr cDNAs were cloned into yeast expression vector pYES2.1/V5-His-TOPO and mammalian expression vector pcDNA3.1/V5-His-TOPO (Invitrogen) as described previously(18).For expression studies in Drosophila S2 cells ...
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bioRxiv - Microbiology 2023Quote: ... whereas His-tagged EF-P was detected with primary monoclonal antibodies against the His-Tag (1:10,000; AB_2536841, Invitrogen). Membranes were incubated with the secondary antibodies conjugated with a fluorophore (1:20,000 in TBS ...
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bioRxiv - Immunology 2023Quote: ... M-IgA was purified by using Peptide M Agarose (ThermoFisher Scientific). S-IgA was purified by two-step chromatography using the Capturem His-Taged Purification Kit (Takara Bio ...
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bioRxiv - Pathology 2021Quote: CSF sPDGFRβ concentration was measured by sandwich ELISA (Thermo Fisher Scientific, USA), as previously described17 ...
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bioRxiv - Microbiology 2022Quote: ... and (iii) CSF sample treated with PrepMan™ Ultra (Thermo-Fisher Scientific) and heated at 100°C for 10 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... The fluorescent CSF tracer (bovine serum albumin, Alexa Fluor 647 conjugate, Invitrogen, Life Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... CNO was dissolved in 0.5% DMSO in artificial CSF (ACSF, Fisher Scientific; 1mM/0.3 µl ...
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bioRxiv - Cancer Biology 2024Quote: ... directed against CSF-1 or negative control (cat#12935300, Thermo Fisher Scientific) at a concentration of 200 pmols using Lipofectamine 2000 reagent (cat# 11668027 ...
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bioRxiv - Genetics 2021Quote: ... 12 µL Hi-Di Formamide (Applied Biosystems) and 0.3 µL MapMarker 500 labelled with DY-632 (Eurogentec) ...
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bioRxiv - Microbiology 2019Quote: ... we used Dynabeads™ His-Tag (Invitrogen) for Ni-affinity purification ...
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bioRxiv - Bioengineering 2022Quote: 6x-His tag antibodies (Invitrogen, MA1-21315) were coated at 2 µg/mL in PBS onto 96-well half- area high binding plates (Corning ...
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bioRxiv - Immunology 2021Quote: ... blot using an anti-His Ab (ThermoFisher). MSP-142 and AMA-1 were biotinylated and tetramerized with streptavidin-PE (SA-PE ...
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bioRxiv - Microbiology 2021Quote: ... eluted in Hi-Di formamide (Applied Biosystems) with ROX-350 ladder ...
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bioRxiv - Biochemistry 2021Quote: ... anti-His (MA1-21315, Invitrogen, 1:1,000). Proteins separated by SDS-PAGE were detected following the manufacturer’s guidelines (GE Healthcare ECL Western Blotting detection system ...
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bioRxiv - Microbiology 2020Quote: Hi-sensitivity Qubit DNA kit (Thermo Fisher) was used to measure the DNA concentration directly from the lysate ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% HI FBS (Gibco 10500), 20% D4T conditioned medium (24) ...
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bioRxiv - Microbiology 2020Quote: ... and cloned into pcDNA-V5/His (Invitrogen). The M fragment of CCHFV was PCR amplified with primers shown in Table II from cDNA generated from CCHFV genomic RNA ...
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bioRxiv - Microbiology 2021Quote: ... mouse anti-His (MA1-21315, Thermo Scientific), mouse anti-β-actin HRP (Santa Cruz Biotechnologies ...
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bioRxiv - Biochemistry 2020Quote: ... was loaded onto His Dynabeads (Invitrogen 10103D), relying on the intrinsic metal-binding properties of untagged PrP ...
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bioRxiv - Zoology 2019Quote: ... Primary (rabbit anti-His, Thermo Fisher Scientific) and secondary (anti-rabbit Alexa Fluor 594 ...
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bioRxiv - Synthetic Biology 2020Quote: ... supplemented with 10% HI FBS (Thermo Fisher). 30,000 HEK293AAV cells were added to each well of a 48 well plate one day before transfection ...
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bioRxiv - Cell Biology 2020Quote: ... 6x-His Tag antibody is from ThermoFisher Scientific (MA1-21315-D550) ...
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bioRxiv - Immunology 2020Quote: ... with 10 % V/V Hi FCS (Gibco), 1 mM sodium pyruvate (Gibco ...
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bioRxiv - Biochemistry 2021Quote: ... terbium-coupled anti-his at 1nM (Invitrogen) and decreasing concentrations of compounds in 384-well microplates (Corning ...
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bioRxiv - Biochemistry 2021Quote: ... mouse anti-His Tag (Invitrogen MA1-21315). Secondary antibody used are ...
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bioRxiv - Neuroscience 2021Quote: ... HI Horse Serum (Invitrogen, 2% vol/vol), GlutaMax (2 mM ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 17.4 μl Hi-Di Formamide (Applied Biosystems), and 2 μL of RT reaction mixture were loaded in a 96-well plate ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Anti-His antibody was from Thermo Fisher Scientific (37-2900 ...
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bioRxiv - Microbiology 2022Quote: ... mouse monoclonal anti-6x-His (Thermo Fisher) diluted 1:1,000 ...
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bioRxiv - Cell Biology 2022Quote: ... anti-His tag (ThermoFisher Scientific, MA1-21315), and anti-tubulin (DHSB ...
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bioRxiv - Zoology 2022Quote: ... AeStaufen cDNA synthesized by Genscript was cloned into pIZT/ V5-His (Invitrogen). The same plasmid containing Chloramphenicol acetyltransferase (CAT ...