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Citations for Thermo Fisher :
251 - 300 of 7362 citations for KLK4 Human H197Q HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... Reporter plasmids (2 μg) were transfected into HEK-293 cells in 6-well plates at ∼60-90% confluency using Lipofectamine 3000 (ThermoFisher Scientific). Cells were harvested after 1 day by aspirating the media and resuspending the cells in 1 mL Trizol reagent (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... HEK-293 cells were maintained at 37°C with 5% CO2 in DMEM/F12 medium (Gibco, Life Technologies, Grand Island, NY) supplemented with 10% heat-inactivated FBS (Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... HEK-293 cells were maintained at 37°C with 5% CO2 in DMEM/F12 medium (Gibco, Life Technologies, Grand Island, NY) supplemented with 10% heat-inactivated FBS (Gibco) ...
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bioRxiv - Biochemistry 2022Quote: The pEG BacMam plasmids with WT or mutant Fpn or the empty plasmid were transfected into HEK 293S cells on black wall 96-well microplates coated with poly-D-lysine (Invitrogen/Thermo Fisher). After 2 days ...
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bioRxiv - Biochemistry 2022Quote: The pEG BacMam plasmids with HsFpn or the empty plasmid were transfected into HEK 293S cells on black wall 96-well microplates coated with poly-D-lysine (Invitrogen/Thermo Fisher). After 2 days ...
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bioRxiv - Cell Biology 2022Quote: ... MEFs were cultured in the same medium as HEK 293 cells but supplemented with 1x non-essential amino acids (Gibco, 11140050). All cells were kept at 37°C in a 5% CO2 humidified atmosphere.
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bioRxiv - Cell Biology 2023Quote: ... HEK 293-TrkB cells were treated with drugs prior to cell lysis with cold Pierce RIPA lysis buffer (Thermo Scientific, 89901) supplemented with protease inhibitor set I (Sigma ...
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bioRxiv - Cell Biology 2023Quote: For proximity ligation assay transfected HEK-293 cells were seeded in an appropriate dilution on Nunc Chamber Slides (Thermo Fisher, USA). After fixation in 4% paraformaldehyde for 15 min at RT ...
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bioRxiv - Bioengineering 2024Quote: ... and m6A-Tracer-NES plasmid (m6A-Tracer; addgene, #159607) were simultaneously transfected into HEK-293 cells using Lipofectamine 3000 Transfection Reagent (Invitrogen, #L3000008). After 48-h co-transfection ...
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bioRxiv - Bioengineering 2020Quote: Adherent human embryonic kidney cells (HEKs) were grown in DMEM (Gibco, Gaithersburg, MD) + 10% FBS ...
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bioRxiv - Genomics 2022Quote: Human HEK 293T cells (ATCC # CRL-3216) were grown in DMEM (ThermoFisher #11995073) supplemented with 10% FBS (ThermoFisher #A3840001 ...
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bioRxiv - Molecular Biology 2023Quote: ... Human HEK 293T Lenti-X cells were grown in DMEM + GlutaMax (ThermoFisher Scientific) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2024Quote: Human Embryonic Kidney (HEK) 293T cells were cultured in DMEM high glucose (Invitrogen), supplemented with 10% fetal bovine serum (GE Healthcare) ...
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bioRxiv - Molecular Biology 2021Quote: ... Stable integration of CLIP-UPF1SL into human Flp-InTM T-RExTM-293 cells (Invitrogen) and subsequent maintenance of this stable line was previously described (Kishor et al ...
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bioRxiv - Immunology 2019Quote: ... Human and rabbit Abs were transiently expressed with the FreeStyle 293 Expression System (Invitrogen). Abs were purified using affinity chromatography (Protein A Sepharose Fast Flow ...
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bioRxiv - Immunology 2019Quote: ... Human and rabbit Abs were transiently expressed with the FreeStyle 293 Expression System (Invitrogen). Abs were purified using affinity chromatography (Protein A Sepharose Fast Flow ...
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bioRxiv - Molecular Biology 2021Quote: Flp-In T-Rex 293 (human, female, embryonic kidney, epithelial; Thermo Fisher Scientific, RRID:CVCL_U427) cells were cultured in high-glucose ...
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bioRxiv - Systems Biology 2020Quote: ... 293-H and Freestyle™ 293-F (Gibco) were obtained from Thermo Fisher Scientific and cultivated in 293 SFM II medium (Gibco ...
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bioRxiv - Neuroscience 2023Quote: The plasmid for heterologous expression of fshr-1 in HEK cells was obtained by directionally cloning the fshr-1 cDNA into the pcDNA3.1/V5-His-TOPO vector (Invitrogen). The cDNA sequence of the fshr-1a gene isoform was amplified by PCR using cDNA from mix-staged wild-type C ...
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bioRxiv - Neuroscience 2021Quote: ... 3-3.5 µg prestin plasmids and 0.4 µg eGFP plasmid were transfected to HEK 293 cells using 10 µl of Lipofectamine ® 3000 (reagent 2:1 ratio; ThermoFisher Scientific) in 500-ml Opti-MEM (Life Technologies) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: HEK-293 cells or HEK-293 cells stably expressing PAR4-YFP were loaded with calcium sensitive dye (Fluo-4 NW, F36206, Life Technologies, Thermo Fisher) and aliquoted into cuvettes with Hanks buffered salt solution (HBSS containing Ca2+ and Mg2+) ...
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bioRxiv - Bioengineering 2021Quote: ... WS1 and HEK-293 cells were obtained from ATCC (Manassas, VA) and cultured in Eagle’s Minimum Essential Medium (Life Technologies, Carlsbad, CA) supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2021Quote: ... HEK 293 cells or rNLS mice brains were lysed in lysis buffer (50 mM Tris-HCl, protease inhibitors cocktail (Thermo Fisher Scientific), phosphatase inhibitor (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... HEK-293 cells (ATCC) were maintained at 37°C under 5% CO2 in Dulbecco’s modified Eagle’s medium (Gibco, Cat. no. 12800-017) supplemented with 10% FBS (Gibco ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... HEK-293 cells were transferred with a density of 12000 cells per well in white 96-well plates (Thermo Scientific™ Nunc™ F96 MicroWell™ ...
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bioRxiv - Biochemistry 2022Quote: The pEG BacMam plasmids with WT or mutant Fpn or the empty plasmid were transfected into HEK 293S cells on black wall 96-well microplates coated with poly-D-lysine (Invitrogen/Thermo Fisher). After 2 days ...
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bioRxiv - Biochemistry 2022Quote: The pEG BacMam plasmids with HsFpn or the empty plasmid were transfected into HEK 293S cells on black wall 96-well microplates coated with poly-D-lysine (Invitrogen/Thermo Fisher). After 2 days ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting plasmids with Fpn or the empty plasmid were transfected into HEK 293S cells on 6-well plates with 293fectin™ transfection reagent (Invitrogen/Thermo Fisher) per the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: HeLa and HEK 293 cell lines were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Life Technologies, Thermo Fisher Scientific, Waltham MA, USA), while SH-SY5Y cells were cultured in 1:1 DMEM:Ham’s F12 (MSD ...
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bioRxiv - Genetics 2023Quote: ... Reverse transfection in HEK 293 cells was performed with 3 nM final siRNA concentration with Lipofectamine™ RNAiMAX (ThermoFisher, Cat. No. 13778075) according to manufactural procedure ...
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bioRxiv - Immunology 2023Quote: ... Consensus and Vietnam matrix protein 2 (M2)-inducible HEK cells were generated using the Flp-In™ T-REx™ 293 Cell Line system (Invitrogen) and grown following the methods in the previous papers 53 ...
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bioRxiv - Neuroscience 2023Quote: ... with fewer than 15 passages onto 12-well plates in HEK-293 growth medium (DMEM [Gibco # 10566-061] with 10% FBS [Gibco #16140-071] and 1x Pen Strep [Gibco #15070-63]) and transfected them when 50-70% confluent ...
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bioRxiv - Immunology 2023Quote: ... Resulting plasmid DNA was transiently transfected into HEK Expi293-F cell line using the ExpiFectamine™ 293 Transfection Kit (Thermo Fisher Scientific). Six days after transfection ...
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bioRxiv - Bioengineering 2023Quote: HEK 293 cells were cultured at 37 °C and 5% CO2 using DMEM high glucose without phenol red (Gibco, Thermo Fisher Scientific), supplemented with 10% fetal bovine serum ...
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bioRxiv - Bioengineering 2023Quote: HEK 293 cells were cultured at 37 °C and 5% CO2 using DMEM high glucose without phenol red (Gibco, Thermo Fisher Scientific), supplemented with 10% fetal bovine serum ...
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bioRxiv - Biochemistry 2024Quote: HEK-293 cells (ATCC, CRL-1573) were subcultured every 3-4 days in Dulbecco’s modified Eagle’s medium (Thermo Fisher Scientific, 41965-039) supplemented with 10% v/v fetal bovine serum (Sigma Aldrich ...
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bioRxiv - Bioengineering 2021Quote: Human embryonic kidney (HEK)293T cells (ATCC, CRL-11268) were grown in DMEM (Gibco) plus 10% heat-inactivated fetal calf serum (FCS ...
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bioRxiv - Neuroscience 2021Quote: Human embryonic kidney (HEK) 293T cells were cultured in DMEM (Gibco, Thermo Fisher Scientific) supplemented with 10% FBS (Gibco ...
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bioRxiv - Neuroscience 2019Quote: Human epithelial kidney (HEK 293T) cells were maintained in Dulbecco’s Modified Eagle’s Medium (Gibco) supplemented with 10% fetal bovine serum (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: Human embryonic kidney (HEK) 293T cells were cultured in DMEM (Gibco, Thermo Fisher Scientific) supplemented with 10% FBS (Gibco ...
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bioRxiv - Cancer Biology 2021Quote: Human embryonic kidney (HEK)293T cells (ATCC, CRL-11268) were grown in DMEM (Gibco) plus 10% heat-inactivated fetal calf serum (FCS ...
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bioRxiv - Synthetic Biology 2021Quote: The human embryonic kidney (HEK) 293T cell line was cultured in DMEM medium (Invitrogen) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2020Quote: ... human embryonic kidney HEK 293T and 293FT epithelial cells were cultured in DMEM (Gibco) supplemented with 2 mM L-Alanyl-L-Glutamine ...
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bioRxiv - Immunology 2020Quote: Human embryonic kidney (HEK) 293T cells (ATCC, CRL-11268) were grown in DMEM (Gibco) plus 10% heat-inactivated fetal calf serum (FCS ...
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bioRxiv - Immunology 2022Quote: ... Proteins were then produced in human embryonic kidney (HEK)293F cells (Thermo Fisher Scientific) maintained in Freestyle medium (Life Technologies) ...
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bioRxiv - Genetics 2023Quote: Human embryonic (HEK)-293T cells were cultured in 10% FBS DMEM (Gibco 11965-092), supplemented with penicillin (50 U per mL ...
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bioRxiv - Biophysics 2021Quote: Wild-type SCN5A (Q1077del NaV1.5)16 was co-transfected with pEGFP-C1 into HEK-293 cells with Lipofectamine 3000 reagent (Thermo Fisher Scientific, Massachusetts, USA).
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bioRxiv - Pharmacology and Toxicology 2019Quote: HEK-293 cells or HEK-293 cells stably expressing PAR4-YFP were loaded with calcium sensitive dye (Fluo-4 NW, F36206, Life Technologies, Thermo Fisher) and aliquoted into cuvettes with Hanks buffered salt solution (HBSS containing Ca2+ and Mg2+) ...
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bioRxiv - Neuroscience 2020Quote: Destabilized EGFP reporter with or without 3’UTR of interest was transduced into HEK 293 cells to establish a stable reporter containing cell line with Blasticidin S HCl (Life Technologies, A11139-03) selection ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting plasmids with Fpn or the empty plasmid were transfected into HEK 293S cells on 6-well plates with 293fectin™ transfection reagent (Invitrogen/Thermo Fisher) per the manufacturer’s protocol ...