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Citations for Thermo Fisher :
101 - 150 of 7362 citations for KLK4 Human H197Q HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Structural and mechanistic insights into disease-associated endolysosomal exonucleases PLD3 and PLD4bioRxiv - Biochemistry 2023Quote: ... incubated 20 min at RT and added to 500 mL 3×106/mL HEK 293S cells culture in Freestyle 293 expression medium (ThermoFisher). The cells were cultured for 5 days ...
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bioRxiv - Immunology 2020Quote: ... Recombinant His-HMGB1 was produced in 293-freestyle cells (ThermoFisher Scientific). Purification of His-HMGB1 from 293-freestyle cell lysate was carried out using Ni SepharoseTM 6 Fast Flow gel (GE Healthcare) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... HEK 293 FlpIn cells were originally obtained from Life Technologies (now Thermofisher, #75007).
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bioRxiv - Cancer Biology 2019Quote: ... HEK-293 cells were cultured in MEM media (Thermo Fisher Scientific, catalog #11095080,) supplemented with 10% FBS (fetal bovine serum ...
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bioRxiv - Microbiology 2021Quote: HEK 293F cells were cultured in FreeStyle™ 293 Expression Medium (Life Technologies) at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2020Quote: HEK 293 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco, USA) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Microbiology 2020Quote: ... The virus was rescued and propagated in T-Rex 293 HEK cells (Invitrogen) which repress antigen expression during virus propagation ...
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bioRxiv - Microbiology 2020Quote: ... Antibodies were transiently expressed in FreeStyle™ HEK 293‐F cells (Life Technologies) using 293fectin transfection reagent according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... suspension-adapted HEK-293T cells grown in FreeStyle 293 Expression Medium (Thermo Fisher) supplemented with 1% IFS were transiently transfected with cDNAs encoding FLAG-tagged Rap2A or FLAG-tagged wild-type ...
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bioRxiv - Biophysics 2022Quote: ... was used to conduct protein expression in HEK 293-F cells (Gibco, USA) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: HEK 293 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco, USA) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Cell Biology 2020Quote: Human embryonic kidney 293 cells (ThermoFisher, R70507), human cervical cancer HeLa cell line (ATCC) ...
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bioRxiv - Biophysics 2021Quote: ... G protein BRET assays were performed as previously described (32367019) with the following modifications: HEK-293S cells grown in FreeStyle 293 suspension media (Thermo Fisher) were transfected at a density of 1 million cells/mL in 2 mL volume using 1200 ng total DNA at 1:1:1:1 ratio of receptor:Gα:Gβ:Gγ and a DNA:PEI ratio of 1:5 ...
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bioRxiv - Microbiology 2020Quote: ... suspension-adapted and serum-free HEK 293S cells were seeded at 3 x 106 cells/mL in Freestyle-293 medium (ThermoFisher Scientific). Next ...
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bioRxiv - Biophysics 2022Quote: BRET assays were performed and analyzed as previously described4 with the following modifications: HEK-293S cells grown in FreeStyle 293 suspension media (Thermo Fisher) were transfected at a density of 1 million cells/mL in 2 mL volume using 600 ng total DNA at 1:1 ratio of Receptor-rLuc:Nb6-mVenus and a DNA:PEI ratio of 1:5 ...
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bioRxiv - Immunology 2023Quote: ... Resulting expression constructs were transiently transfected or co-transfected into HEK Expi-293-F cells with the ExpiFectamine 293 transfection kit (ThermoFisher, A14525), according to company protocol ...
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bioRxiv - Biochemistry 2021Quote: Human embryonic kidney HEK-293FT cells (Invitrogen) were cultivated in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Cell Biology 2022Quote: ... and neonatal human epidermal keratinocytes (HEK, GIBCO). HDFn were cultured in high glucose Dulbecco’s Modified Eagle medium (DMEM ...
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bioRxiv - Biochemistry 2020Quote: ... HEK 395F cells were grown in unsupplemented Freestyle 293 media (Life Technologies, 12338-018) until cell density reached 2×106 cells/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... To generate stably-expressing HEK-293 cell lines 150 μg/ml hygromycin B (Invitrogen) was added to the medium ...
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bioRxiv - Biophysics 2020Quote: HEK 293 cells were maintained in DMEM supplemented with 10% v/v FBS (Gibco) at 37° C (5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... Humanized antibodies were produced by transfecting HEK-293 F (Thermo Fisher Scientific, Waltham, USA) with the respective plasmids with the FreeStyle™ 293 Expression System according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: HEK 293 cells were routinely grown in DMEM/F-12 GlutaMAXTM (Thermo Fisher Scientific) supplemented with 10% foetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2021Quote: HEK 293 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Life Technologies, Switzerland) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2021Quote: ... Suspension-adapted HEK-293F were maintained in serum-free FreeStyle 293 Expression Medium (Gibco). On the day of transfection ...
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bioRxiv - Cell Biology 2023Quote: ... The HEK-293 cells were cultured in Eagle’s Minimum Essential Medium (Thermo Fisher Scientific) supplemented with FBS (Corning ...
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bioRxiv - Biophysics 2022Quote: HEK 293-T cells were cultured with Dulbecco’s modified Eagle’s medium (DMEM) (Gibco, USA) supplemented with 15% (v/v ...
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bioRxiv - Bioengineering 2023Quote: ... HEK 293-6E cells were grown and passaged in F17 media (Fisher Scientific, A1383502) supplemented with glutamine (Invitrogen ...
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bioRxiv - Genetics 2024Quote: ... HEK-293 cells were transfected with respective DNA plasmids and Lipofectamine 2000 (Life Technologies) for 48 hours before lysis ...
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bioRxiv - Cell Biology 2020Quote: ... Human Embryonic Kidney 293 cells (HEK293T – Invitrogen, UK) were grown in Dulbecco’s modified Eagle medium (DMEM - Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: Human embryonic kidney cells (HEK-293A, Life Technologies) were cultured in high glucose (4.5 g/L ...
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bioRxiv - Neuroscience 2023Quote: Human embryonic kidney (HEK) 293FT cells (ThermoFisher Scientific) were grown at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... HEK-293 cells were maintained in EMEM with 10% v/v heat-inactivated FBS (Gibco) at 37 °C ...
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bioRxiv - Immunology 2019Quote: ... HEK 293-F cells were transiently transfected using the FreeStyle MAX293 expression system (Life Technologies) and proteins purified as for CHO-K1 cells.
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bioRxiv - Neuroscience 2020Quote: HEK 293 cells (ATCC ref CRL-1573) were transfected using Lipofectamine 2000 (Invitrogen 11668-019) and 2 μg of pcDNA3.1 CMV V5 Csmd1 (Mus Musculus ...
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bioRxiv - Cancer Biology 2019Quote: HEK-293 and A549 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Life Technologies) supplemented with 10% FBS and penicillin-streptomycin ...
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bioRxiv - Biochemistry 2020Quote: HEK-293 cells were maintained in Dulbecco’s modified Eagle medium (DMEM; Gibco, Cat# 31966-021) containing 10% FBS and streptomycin and penicillin (10 μg/ml ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... HEK 293 cell lines were routinely cultured in DMEM/F-12 GlutaMAX (Thermo Fisher Scientific) supplemented with 10% FBS (F9665 ...
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bioRxiv - Cell Biology 2020Quote: HEK 293 cells (ATCC) were cultured in complete growth Dulbecco’s modified Eagle’s medium (DMEM, Gibco) and supplemented with 10% fetal bovine serum (UCSF Cell Culture Facility) ...
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bioRxiv - Neuroscience 2021Quote: ... These plasmids were further co-transfected into pre-grown HEK-293 cells using lipofectamine (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: HEK-293 and U2OS cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco, 11965092) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2023Quote: Human embryonic kidney 293 cells (Expi293F™, Thermo Fisher) were cultured in Expi293™ Expression medium at a cell density between 5 x 105 cells/ml and 4 x 106 cells/ml in suspension ...
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bioRxiv - Cancer Biology 2019Quote: ... PCR8-KLK4-KLKP1 (DDK tagged) was recombined with pLenti6/V5-Dest™ (Life Technologies) or pAD/CMV/V5-Dest™ (Life Technologies) ...
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bioRxiv - Immunology 2020Quote: HEK-293 cells were co-transfected with 100 µM miR29c-5p miRVANA miRNA mimic (4464066, ThermoFisher) using Lipofectamine and one of the following Luciferase vectors ...
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bioRxiv - Bioengineering 2022Quote: ... Suspension-adapted HEK-293F cells were routinely maintained in serum-free FreeStyle 293 Expression Medium (Gibco). On the day of transfection ...
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bioRxiv - Microbiology 2021Quote: ... HEK 293 cells and CaCo-2 cells were cultured in DMEM/F-12 and DMEM (Gibco), respectively ...
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bioRxiv - Microbiology 2021Quote: RNA was isolated from transfected HEK 293 cells with TRIzol® Reagent (Thermo Fisher Scientific, #15596026) and DNase-treated with TURBO™ DNase (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... while HEK-293-T cells were maintained in Dulbecco’s modified Eagle medium (DMEM) (Thermo Fisher Scientific). All cell culture media were supplemented with 10% (volume/volume ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... HEK 293 cell lines were routinely cultured in DMEM/F-12 GlutaMAX™ (Thermo Fisher Scientific) supplemented with 10% FBS (F9665 ...
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bioRxiv - Bioengineering 2021Quote: ... Suspension-adapted HEK-293F cells were maintained in serum-free Free Style 293 Expression Medium (Gibco). On the day of transfection ...