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Citations for Thermo Fisher :
651 - 700 of 10000+ citations for Interstitial collagenase MMP 1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... and cloned into pcDNA-V5/His (Invitrogen). The M fragment of CCHFV was PCR amplified with primers shown in Table II from cDNA generated from CCHFV genomic RNA ...
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bioRxiv - Microbiology 2021Quote: ... mouse anti-His (MA1-21315, Thermo Scientific), mouse anti-β-actin HRP (Santa Cruz Biotechnologies ...
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bioRxiv - Biochemistry 2020Quote: ... was loaded onto His Dynabeads (Invitrogen 10103D), relying on the intrinsic metal-binding properties of untagged PrP ...
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bioRxiv - Zoology 2019Quote: ... Primary (rabbit anti-His, Thermo Fisher Scientific) and secondary (anti-rabbit Alexa Fluor 594 ...
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bioRxiv - Synthetic Biology 2020Quote: ... supplemented with 10% HI FBS (Thermo Fisher). 30,000 HEK293AAV cells were added to each well of a 48 well plate one day before transfection ...
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bioRxiv - Cell Biology 2020Quote: ... 6x-His Tag antibody is from ThermoFisher Scientific (MA1-21315-D550) ...
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bioRxiv - Immunology 2020Quote: ... with 10 % V/V Hi FCS (Gibco), 1 mM sodium pyruvate (Gibco ...
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bioRxiv - Biochemistry 2021Quote: ... terbium-coupled anti-his at 1nM (Invitrogen) and decreasing concentrations of compounds in 384-well microplates (Corning ...
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bioRxiv - Biochemistry 2021Quote: ... mouse anti-His Tag (Invitrogen MA1-21315). Secondary antibody used are ...
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bioRxiv - Neuroscience 2021Quote: ... HI Horse Serum (Invitrogen, 2% vol/vol), GlutaMax (2 mM ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 17.4 μl Hi-Di Formamide (Applied Biosystems), and 2 μL of RT reaction mixture were loaded in a 96-well plate ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Anti-His antibody was from Thermo Fisher Scientific (37-2900 ...
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bioRxiv - Microbiology 2022Quote: ... mouse monoclonal anti-6x-His (Thermo Fisher) diluted 1:1,000 ...
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bioRxiv - Cell Biology 2022Quote: ... anti-His tag (ThermoFisher Scientific, MA1-21315), and anti-tubulin (DHSB ...
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bioRxiv - Zoology 2022Quote: ... AeStaufen cDNA synthesized by Genscript was cloned into pIZT/ V5-His (Invitrogen). The same plasmid containing Chloramphenicol acetyltransferase (CAT ...
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bioRxiv - Molecular Biology 2023Quote: ... The antibody against His (631212, Fisher Scientific) is commercially available ...
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bioRxiv - Neuroscience 2023Quote: The plasmid pcDNA6.1/V5-His B (Thermofisher) was used as the template to subclone in the full-length PNPLA6 cDNA (from RefSeq transcript NM_001166111.1) ...
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bioRxiv - Microbiology 2023Quote: ... and probed with □-His mAb (Thermo Scientific) diluted 1:1,000 in 6% milk in PBS-T followed by α-mouse IgG HRP (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... His Pur cobalt spin columns (Thermo Scientific) were used to purify the in vitro-translated proteins ...
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bioRxiv - Microbiology 2024Quote: ... 10% HI-FBS (v/v, Gibco 10082147) 100 U/mL penicillin ...
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bioRxiv - Immunology 2024Quote: ... Trichoplusia ni (Hi-5, Thermo Fisher Scientific) and Spodoptera fugiperda (Sf9 ...
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bioRxiv - Cancer Biology 2022Quote: ... Gels were chopped into smaller pieces to increase their surface area and incubated with 1:1 ratio of collagen to type I collagenase (Gibco) dissolved in PBS at 2 mg/ml ...
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bioRxiv - Neuroscience 2023Quote: ... iPS cells were passaged once a week (∼80% confluence/well) at 1:4-1:6 ratios using 1mg/ml collagenase (Gibco) to detach colonies and onto fresh ir-MEF plates.
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bioRxiv - Immunology 2023Quote: ... and resuspended with 5 mg/mL collagenase and 1 mg/mL DNase I in 1 mL of RPMI 1640 without phenol red (Gibco). After incubation at 37°C for a half hour ...
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bioRxiv - Cell Biology 2023Quote: ... Cells in the liver were obtained by gentle grinding with slide glasses followed by digestion at 37°C for 30 min in 1 mg ml−1 collagenase type IV (17104019, Gibco), 2 mg ml−1 dispase (17105041 ...
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bioRxiv - Cell Biology 2020Quote: ... minced into 1-2 mm squares and digested with Collagenase Type I (2 mg/ml, Gibco) at 37°C for 1 hour with agitation ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were passaged as cell clumps by dissociating with collagenase IV (1 mg/ml; Invitrogen), and maintained in the medium described as above but supplemented with 20% KSR ...
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bioRxiv - Molecular Biology 2022Quote: ... Cultures were passaged every 5 to 7 days with collagenase type IV (Invitrogen; 1 mg/mL). The identities of all parental hESC and hiPSC lines were confirmed by DNA fingerprinting and all cell lines were regularly tested to exclude mycoplasma contaminations using a PCR-based assay ...
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bioRxiv - Neuroscience 2020Quote: ... and then digested to isolating cells with type-I collagenase (1 mg/mL, Thermo Fisher Scientific) and dispase II (5 mg/mL ...
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bioRxiv - Cell Biology 2019Quote: ... minced into 1-2 mm squares and digested with Collagenase Type I (2 mg/ml, Gibco) at 37°C for 2 h with occasional agitation ...
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bioRxiv - Physiology 2019Quote: ... followed by two incubations in digestion buffer [collagenase Type 1 at 145 units/mL (Life Technologies) with DNase I at 25 units/µL (Invitrogen ...
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bioRxiv - Immunology 2019Quote: ... Tissue was digested for 45 minutes in 0.3% Type 1 Collagenase and 0.4% Dispase II (Gibco) in Roswell Park Memorial Institute (RPMI ...
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bioRxiv - Developmental Biology 2021Quote: Seminiferous tubules were isolated by enzymatic digestion (0.75-1 mg/ml collagenase IV, Gibco 17104-019) for 10 min in a 37°C water bath as described previously (Bryant et al. ...
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bioRxiv - Immunology 2024Quote: ... followed by 1 hr incubation with 150 U/mL collagenase (Life Technologies, cat#17-104-019)) diluted in RPMI (Life Technologies ...
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bioRxiv - Biochemistry 2021Quote: 1-2 μg His-tagged calcineurin was first bound to magnetic Dynabeads (Thermo Fisher Sci. USA) in base buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Immunology 2021Quote: ... with 10% FBS (Nucleus Biologics, #AU FBS-500ml L1 HI) and 1% Penicillin-Streptomycin (Gibco, #15140122) and transferred to a new tube ...
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bioRxiv - Developmental Biology 2021Quote: ... decapsulated and transferred in low-binding Eppendorf tube containing 100-150µL collagenase solution in HBSS (Collagenase type IV (Gibco), 2X AAs (Gibco) ...
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bioRxiv - Genetics 2021Quote: ... We dissociated the brains as previously described after incubation with 450μl of collagenase solution (50 ul of fresh 25mg/ml collagenase (Gibco) in sterile water + 400μl of Schneider’s medium ...
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bioRxiv - Cell Biology 2022Quote: ... the human organoids were cultured with 1:1 mix of Human IntestiCult™ OGM Basal Medium and DMEM/F-12 (ThermoFisher) in the presence of 100 IU Penicillin and 100 μg/ml Streptomycin ...
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bioRxiv - Immunology 2024Quote: ... blocked then incubated overnight at 4°C in primary antibody (rabbit anti-human STAT1 1:500, HPA000931, Sigma-Aldrich; mouse anti-human pSTAT1 1:1000, 33-3400, Invitrogen; mouse anti-human STAT2 ...
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bioRxiv - Molecular Biology 2021Quote: 10,000 HEK293 FRT were seeded in 96 well plates (M33089, ThermoFisher UK) and induced with tetracycline for 24 hours ...
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bioRxiv - Genomics 2019Quote: Total RNA was prepared from harvested knockdown HEK293 cells using TRIzol (ThermoFisher). Single-stranded DNA in the sense orientation was synthesized from total RNA with random priming using the GeneChip WT Amplification kit (Affymetrix) ...
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bioRxiv - Molecular Biology 2021Quote: HEK293T and HEK293 cells were cultured in DMEM 1X (Thermo Fisher Scientific) supplemented with 0.5 % Peniciline/Streptomycin 100X Solution (Biowest ...
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bioRxiv - Cell Biology 2019Quote: HeLa and HEK293 cell samples prepared in 6-well plates (Fisher Scientific) were used for the assay ...
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bioRxiv - Genetics 2019Quote: ... Plasmids were transfected into podocytes or HEK293 cells using Lipofectamine 2000 (Invitrogen). HEK293 cells stably expressing p3xFLAG-ADCK4 or BAP were selected and maintained with 1 mg/mL G418.
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bioRxiv - Neuroscience 2021Quote: HEK293 cell line were plated in 24-well plates in DMEM (Invitrogen) supplemented with 10% fetal bovine serum ...
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bioRxiv - Systems Biology 2021Quote: ... Ptk2 and HEK293 cells were incubated in imaging medium (FluoroBrite DMEM (Gibco) supplemented with 10 % FBS (Gibco) ...
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bioRxiv - Biochemistry 2021Quote: ... plasmids were transfected into HEK293-T cells using Lipofectamine 2000 (Life Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2020Quote: ... The tetracycline inducible cell line T-REx™-HEK293 (Thermo Fisher Scientific) was cultured under the following conditions ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmids were transfected into HEK293 cells using either Lipofectamine 2000 reagent (Invitrogen) or calcium phosphate transfection.