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Citations for Thermo Fisher :
601 - 650 of 10000+ citations for Interstitial collagenase MMP 1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... pX330 plasmid harboring a p53-specific gRNA (Supplementary Table 1) was transfected into HEK293 and HCT116 cells using Lipofectamine 2000 (Invitrogen). Two days later ...
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bioRxiv - Microbiology 2021Quote: ... Transfections were carried out using 1 mg of total DNA (500 μg each of VH and Vκ plasmid DNA) and 1 l of cultured HEK293-F cell suspension maintained in sterile Freestyle 293 expression medium (Invitrogen) without antibiotics at 37 °C ...
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bioRxiv - Biochemistry 2024Quote: ... plasmid DNA into 1 × 106 HEK293 cells plated per well of a 6-well plate using Lipofectamine 2000 transfection reagent (Invitrogen). Each well was transfected with 2 µg of total plasmid DNA (50 ng Cg4-puro ...
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bioRxiv - Genomics 2022Quote: Human total RNA was extracted from THP-1 human cells using TRIzol (ThermoFisher, cat# 15596026) as per manufacturer instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... His-tagged GOT2 protein was immunoprecipitated using the His-tag isolation and pull-down Dynabeads system (Thermo Fisher) using the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and further ligated into pcDNA 3.1 V5/His TOPO vector using pcDNA 3.1 V5/His TOPO TA cloning kit (Invitrogen). The ACRD etoll was then transferred to pGADT7-AD vector using Nde1 and BamH1 sites ...
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bioRxiv - Molecular Biology 2023Quote: ... ceSMSγ and ceSMSr cDNAs were cloned into yeast expression vector pYES2.1/V5-His-TOPO and mammalian expression vector pcDNA3.1/V5-His-TOPO (Invitrogen) as described previously(18).For expression studies in Drosophila S2 cells ...
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bioRxiv - Systems Biology 2020Quote: ... 2 mg/mL collagenase type II (Gibco) in DMEM for 60 minutes at 37oC with inversion of the tubes to mix every 5 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... 42.000 U Collagenase type 2 (Life technologies) and 23.000 U DNase (Life technologies) ...
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bioRxiv - Physiology 2021Quote: ... 0.025% (w/v) collagenase type IV (Gibco) and 5 mM CaCl2 was added to Leffert perfusion buffer containing 10 mM HEPES ...
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bioRxiv - Cell Biology 2020Quote: ... and type IV collagenase (Gibco,17104-019). Glands were shaken at 37°C for 35 min ...
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bioRxiv - Microbiology 2021Quote: ... and Collagenase IV (4.8 mg/ml, Invitrogen) in PBS at 37°C for 45–60 min ...
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bioRxiv - Developmental Biology 2021Quote: ... Collagenase and FBS were from Gibco (Australia). FITC Phalloidin FITC and TRITC Phalloidin rhodamine were from Yeasen (Cat#40735ES75 ...
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bioRxiv - Genomics 2019Quote: ... 1000 U/mL collagenase type II (Invitrogen) and 10 mg/mL DNaseI (Roche) ...
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bioRxiv - Immunology 2022Quote: ... and 2 mg/ml collagenase 8 (Gibco) for 45 min at 37 °C ...
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bioRxiv - Cancer Biology 2019Quote: ... and 100U/ml Collagenase Type I (Invitrogen); this was incubated for 45min in rotation at 37°C ...
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bioRxiv - Molecular Biology 2019Quote: ... then with collagenase type I (Thermo Fisher) (0.65% ...
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bioRxiv - Genetics 2020Quote: iPS were digested with Collagenase IV (Gibco), and neural induction was induced by switching from ESC growth media to differentiation media in suspension culture [Advance DMEM/F12(Invitrogen) ...
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bioRxiv - Cancer Biology 2021Quote: ... and digested with collagenase type I (Gibco). All samples were stained with Zombie Aqua (BioLegend ...
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bioRxiv - Cancer Biology 2020Quote: ... re-suspended in 0.5mg/ml collagenase (ThermoFisher) and digested with shaking (55rpm for 75 mins at 37°C) ...
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bioRxiv - Immunology 2021Quote: ... digested in 0.2% Collagenase II (Life Technologies) and 0.2% Collagenase V (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... 250 U/mL collagenase IV (Life Technologies), 100μg/mL Primocin ...
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bioRxiv - Cell Biology 2023Quote: ... except that collagenase Type IV (17104019; Gibco) was used instead of collagenase H to minimize unspecific digestion of proteins other than collagen ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1000 U of collagenase II (Thermofisher). Digestion was performed at 37°C during 20 minutes and mechanical dissociation every 5 minutes through 10 back-and-forth passages of the pancreatic tissue into sterile serological pipettes of decreasing size (25 ...
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bioRxiv - Immunology 2023Quote: ... and collagenase XI (250µg/ml; Life Technologies) in RPMI-1640 as described previously72 ...
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bioRxiv - Cell Biology 2023Quote: ... 500 U/mL Collagenase IV (17104019; Gibco) was added to breakdown the scaffold and incubated for 10 minutes at 37ºC ...
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bioRxiv - Immunology 2023Quote: ... and collagenase (10 mg/ml, Life technologies) and incubated for 60 minutes at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... Collagenase 11 (2mg/mL; Gibco; 17101-015), DNAse (20U/mL ...
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bioRxiv - Cell Biology 2023Quote: ... for30 min and 0.25% collagenase II (Invitrogen) at 37°C for 6 h sequentially ...
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bioRxiv - Immunology 2023Quote: ... 0.25% collagenase IV (17104019, Thermo Fisher Scientific) and 15 μg/mL DNase (D4527-10KU ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... A mix of Collagenase II (Thermo Fisher), Dispase II (Solarbio) ...
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bioRxiv - Genomics 2024Quote: ... or collagenase I (Gibco, Thermo Fisher Scientific) in 870 mM HBSS ...
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bioRxiv - Genomics 2024Quote: ... or collagenase I (Gibco, Thermo Fisher Scientific) in 870 mM HBSS ...
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bioRxiv - Systems Biology 2020Quote: ... human buffy coat samples were diluted 1:1 in PBS (Gibco) and mononuclear cells were isolated with a Histopaque-1077 density gradient (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2019Quote: ... polyclonal rabbit anti-mouse/human ZO-1 (1:200; Life Technologies), monoclonal rat anti-mouse ICAM-1 (1:100 ...
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bioRxiv - Neuroscience 2020Quote: ... supplemented with 1% human keratinocyte growth supplement (Gibco), 0.2% GibcoAmphotericin B (250 µg/mL of Amphotericin B and 205 µg/mL sodium deoxycholate ...
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bioRxiv - Cell Biology 2022Quote: ... 1% P/S and human TLR agonists (InvitroGen) in 96-well round bottom plates. ...
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bioRxiv - Immunology 2019Quote: ... anti-human CD45RO (UCHL-1, Thermo Fisher Scientific), anti-mouse CD3 (17A2 ...
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bioRxiv - Neuroscience 2022Quote: ... 10μg ml−1 human recombinant insulin (GIBCO, 12585014).
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bioRxiv - Neuroscience 2023Quote: ... Human tau-specific (HT7, 1:2,000; Invitrogen MN1000) and TaupThr231/pSer235(AT180 ...
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bioRxiv - Cell Biology 2023Quote: ... and 1% Human Keratinocyte Growth Supplement (#S0015 Gibco), 1% Pen/Strep and 2.5 µg/mL amphotericin B ...
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bioRxiv - Cell Biology 2023Quote: ... anti-human PSGL-1-PE (TC2, Life Technologies) at 1:300 ...
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bioRxiv - Cell Biology 2023Quote: ... rabbit anti-human FRα (1:1000 pH9, Thermofisher).
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bioRxiv - Genetics 2021Quote: ... 12 µL Hi-Di Formamide (Applied Biosystems) and 0.3 µL MapMarker 500 labelled with DY-632 (Eurogentec) ...
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bioRxiv - Microbiology 2019Quote: ... we used Dynabeads™ His-Tag (Invitrogen) for Ni-affinity purification ...
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bioRxiv - Bioengineering 2022Quote: 6x-His tag antibodies (Invitrogen, MA1-21315) were coated at 2 µg/mL in PBS onto 96-well half- area high binding plates (Corning ...
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bioRxiv - Immunology 2021Quote: ... blot using an anti-His Ab (ThermoFisher). MSP-142 and AMA-1 were biotinylated and tetramerized with streptavidin-PE (SA-PE ...
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bioRxiv - Microbiology 2021Quote: ... eluted in Hi-Di formamide (Applied Biosystems) with ROX-350 ladder ...
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bioRxiv - Microbiology 2020Quote: Hi-sensitivity Qubit DNA kit (Thermo Fisher) was used to measure the DNA concentration directly from the lysate ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% HI FBS (Gibco 10500), 20% D4T conditioned medium (24) ...