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Citations for Thermo Fisher :
701 - 750 of 10000+ citations for IL 7 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... HEK293 WT cells were transfected with both sgRNA vectors using Lipofectamine 2000 (Invitrogen). 24-hours after transfection cells were subjected to Puromycin selection (2ug/mL ...
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bioRxiv - Neuroscience 2020Quote: HEK293 cells were grown in DMEM medium with fetal bovine serum (Life Technologies) and 1% penicillin/streptomycin (Life Technologies ...
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bioRxiv - Biochemistry 2021Quote: ... HEK293S GnTi- cells were cultured in Freestyle 293 expression medium (Thermo Fisher Scientific) at 37 °C with 5% CO2 ...
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bioRxiv - Biochemistry 2021Quote: ... The HEK293S-GnTi− cells were cultured in Freestyle 293 medium (Thermo Fisher Scientific) supplemented with 5% CO2 at 37 ° C ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK293 and HCT116 cells were cultured in Dulbecco’s Modified Eagle Medium (Gibco, 11995065) with 10% FBS (VWR ...
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bioRxiv - Cell Biology 2020Quote: ... HeLa and HEK293 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) containing 5 - 10% FBS (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... HEK293T and HEK293 cells were grown in Dulbecco’s Modified Eagle Medium (DMEM, Invitrogen) which was supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Neuroscience 2022Quote: HEK293 FT cells were transfected with the BioID2 constructs using Lipofectamine 2000 (Invitrogen) in Opti-MEM ...
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bioRxiv - Physiology 2022Quote: ... HEK293 cells were transfected with human TRPA1 expressing plasmid using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... HEK293 and MC38 cells were incubated in DMEM (Meilunbio) with 10% FBS (Gibco). THP1-dual cells were incubated in RPMI-1640 (Meilunbio ...
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bioRxiv - Biochemistry 2023Quote: HEK293S GnTI− cells were grown at 37°C in Freestyle 293 medium(Invitrogen) supplemented with 1.5% FBS ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmids (3ug per well) were transfected into HEK293 cells using lipofectamine 2000 (Invitrogen), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293S MGAT−/− cells were maintained in a 1:1 ratio of Freestyle (ThermoFisher) and Ex-Cell (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: Human embryonic kidney (HEK293, ATCC #CRL-1573) cells were cultured in DMEM (Gibco), supplemented with 10% (vol/vol ...
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bioRxiv - Cell Biology 2023Quote: HEK293 and HEK293T cells were cultured in DMEM containing Glutamax (Thermo Fisher Scientific) and K562 cells were cultured in RPMI 1640 containing Glutamax (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293 and HEK293T cells were cultured in Dulbecco’s modified Eagle medium (DMEM, Gibco), HCT116 cells were cultured in McCoy’s 5A modified Medium ...
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bioRxiv - Immunology 2022Quote: ... To produce the HEK293 Flp-In T-REx cells (R78007, Thermo Fisher Scientific) cell line with conditional knock-down of CNOT1 we designed a tri-miRNA construct (listed below ...
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bioRxiv - Cell Biology 2023Quote: ... and pMD2G plasmids were transfected into HEK293-T cells using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 or U2OS cells were transfected using Lipofectamine 3000 (Thermo Fisher Scientific L3000015) according to the manufacturer’s instructions ...
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Machine Learning Ensemble Directed Engineering of Genetically Encoded Fluorescent Calcium IndicatorsbioRxiv - Bioengineering 2023Quote: HEK293 cells were cultured in Dulbecco’s Modified Eagle Medium + GlutaMAX (Gibco; 10569-010) supplemented with 10% fetal bovine serum (Biowest ...
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bioRxiv - Cell Biology 2023Quote: ... and suspended in serum free medium HEK293 SFM II (Gibco, catalog #: 11686-029), supplemented with 25 mM HEPES (Gibco ...
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bioRxiv - Cell Biology 2023Quote: Hela and HEK293 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biophysics 2023Quote: Adherent HEK293S GnTI- cells were grown in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... Twin-Streptagged IGF2BP2 was expressed in HEK293 Flp-In T-Rex cells (Invitrogen) by tetracycline induction (200 ng/ml ...
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bioRxiv - Molecular Biology 2023Quote: For HEK293T (ATCC CRL-3216) and HEK293/Flp-In/T-Rex (Invitrogen R71007) cell lines ...
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bioRxiv - Cell Biology 2023Quote: ... the HEK293-derived cells Flp-InTM T-RexTM 293 Cell Line (ThermoFisher Scientific) were stably transfected to express HA-TDP-43 upon induction with tetracycline (1 μg/mL) ...
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Nucleocytoplasmic transport rates are regulated by cellular processes that modulate GTP availabilitybioRxiv - Cell Biology 2023Quote: ... HEK293 Phoenix cells were transfected with the plasmid using Lipofectamine 3000 (ThermoFisher Scientific). The transfected cells were incubated at 37°C for 5 hr ...
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bioRxiv - Developmental Biology 2024Quote: ... HEK293 cells were grown in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific) supplemented with 10% (v/v ...
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bioRxiv - Cell Biology 2024Quote: ... HEK293-T cells were seeded in 10-tray Cell Factories (Thermo Fisher Scientific) 24 hours before transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK293 cells were transfected using Lipofactamine 2000 according to the manufacturer’s instructions (Invitrogen).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... while IL-8 production was evaluated using human IL-8 ELISA kit (Thermo Scientific), with assay range of 2-250 pg/mL.
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bioRxiv - Immunology 2021Quote: ... 5 ng/mL IL-1β and 5 ng/mL IL-23 from Invitrogen (14823163) (Th17.1s ...
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bioRxiv - Cancer Biology 2022Quote: ... Plasma IL-6 was measured by immunoassay (ProQuantum Human IL-6 Immunoassay Kit, ThermoFisher) and run on the ABI 7300 (Applied Biosystems ...
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bioRxiv - Immunology 2022Quote: ... together with IL-4 (100 ng/ml) and IL-21 (50 ng/ml; Invitrogen) for five days.
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bioRxiv - Microbiology 2020Quote: Luminex profiling was performed on whole blood that was allowed to clot for 20 minutes and then spun down using a custom mouse IFN kit (IFN alpha, IFN beta, IL-28, Invitrogen), mouse cytokine 23-plex (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... Luminex kits that were used are as follows a custom mouse IFN kit (IFN alpha, IFN beta, IL-28, Invitrogen), mouse cytokine 23-plex (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: NK cells were enriched and incubated with 50 ng/ml recombinant mouse IL-15 (Preprotech) for four days in RPMI plus 10 % FBS with penicillin and streptomycin (Thermofisher) added in a U-bottom 96-well plate at the concentration of two million cells per ml ...
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bioRxiv - Bioengineering 2022Quote: ... Wells were rinsed with PBS and CD8 T cells were added at 1×106 cells/mL in complete RPMI supplemented with 50 U/mL IL-2 and mouse T-activator Dynabeads (ThermoFisher) at 1:1 ratio ...
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bioRxiv - Immunology 2023Quote: ... Cells were then stained with the following fluorophore-conjugated anti-mouse antibodies for 30 min at 4°C: IL-15Rα-PE (clone DNT15Ra; Invitrogen), CD40-BV421 (clone 3/23 ...
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bioRxiv - Cell Biology 2023Quote: ... commercially available kits were purchased and the assays done following the manufacturer’s protocol: IL-1β (Mouse: BMS6002; Human: BMS224INST, Thermo Fisher) and HMGB1 (Mouse ...
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bioRxiv - Immunology 2023Quote: ... After a 2 day culture with interleukin (IL)-21 and irradiated mouse fibroblast (L cells) expressing CD40-ligand in DMEM F12 media (Gibco), the B cells were immortalized by retroviral transduction with BcL6 ...
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bioRxiv - Immunology 2024Quote: ... The amount of TNF-α and IL-6 in serum was quantified using commercial mouse ELISA kits (Thermo Fisher Scientific). For ex vivo experiments using splenic myeloid cells ...
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bioRxiv - Biochemistry 2019Quote: ... SAMS peptide substrate (His-Met-Arg-Ser-Ala-Met-Ser-Gly-Leu-His-Leu-Val-Lys-Arg-Arg, obtained from Invitrogen, USA) was used for the assay ...
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bioRxiv - Biochemistry 2020Quote: ... the magnetic beads were resuspended in 10.0625 μL ROX/Hi-Di (0.0625 μL of ROX 350 ladder, Applied Biosystems, in 10 μL of Hi-Di formamide, Applied Biosystems) and incubated at room temperature for 20 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... the magnetic beads were resuspended in 10.0625 μL ROX/Hi-Di (0.0625 μL of ROX 350 ladder [Applied Biosystems] in 10 μL of Hi-Di formamide [Applied Biosystems]) and incubated at room temperature for 20 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... Cry1 pcDNA4A-myc-his and Cry2 pcDNA4A-myc-his plasmids were double digested with NotI and XhoI FastDigest enzymes (Thermo Scientific) and used as host plasmids ...
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bioRxiv - Biochemistry 2023Quote: Plasmids of pET28a-his TEV-glnR or pET28a-his TEV-glnR derivative was firstly transformed into expression strain BL21(DE3) (Invitrogen, Inc.). then single colonies of the positive transformants were inoculated and amplified with 5 L LB broth supplemented with 50 μg/ml kanamycin at 37 °C with shaking ...
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bioRxiv - Immunology 2021Quote: ... TNF and IL-6 (Invitrogen) ELISA kits were used according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2019Quote: ... 8ng/mL IL-2 (Gibco), and 50 μM β-mercaptoethanol (MilliporeSigma) ...
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bioRxiv - Immunology 2019Quote: ... IL-1β (Invitrogen, Carlsbad, CA), and macrophage inflammatory protein-2 (MIP-2 ...