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Citations for Thermo Fisher :
601 - 650 of 10000+ citations for IL 7 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... while HEK293 cells were cultured at 37°C in DMEM Medium (Gibco). Both media were supplemented with 10% heat-inactivated fetal bovine serum and 1% penicillin/streptomycin ...
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bioRxiv - Molecular Biology 2023Quote: HEK293 cells were maintained in DMEM/F-12 (Gibco, cat no. 11320033) supplemented with 1% Penicillin-streptomycin (Gibco ...
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bioRxiv - Biophysics 2023Quote: HEK293 cells were transfected with ICD constructs using Lipofectamine 2000 (Thermo Fisher) and were selected with G418 ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293 cells were seeded overnight and transfected with Lipofectamine 3000 (Thermo Fisher) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: HEK293-6E suspension cells were cultured in FreeStyle F17 expression medium (Gibco) supplemented with 4 mM L-glutamine (Gibco) ...
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bioRxiv - Biochemistry 2024Quote: HEK293 Freestyle suspension cells were cultured in HEK Freestyle Media (Invitrogen, 12338018) grown at 37° C in a humidified shaking platform incubator with 10% CO2 ...
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bioRxiv - Bioengineering 2024Quote: HEK293-F suspension cells were cultured in FreeStyle 293 Expression Medium (Gibco) in shake flasks at 135 rpm ...
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bioRxiv - Biochemistry 2024Quote: HEK293 cells (ATCC) were grown in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2020Quote: ... were coated overnight at 4°C with 2 ug/ml of mouse anti-His-tag antibody (Invitrogen cat. #MA1-21315-1MG, Thermo Fisher Scientific) in PBS ...
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bioRxiv - Synthetic Biology 2023Quote: ... Membranes were incubated overnight (50 rpm, 4°C) with either mouse anti-His monoclonal (Thermo Fisher; 1:3000 in 5% skimmed milk) or anti-IFNa2 primary antibody (abcam ...
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bioRxiv - Bioengineering 2023Quote: ... Yeast cells were incubated with 1:1000 dilution of mouse anti-Myc antibody and 1:1000 dilution of chicken anti-His (Invitrogen, PA1-9531) antibodies on ice for 20 min ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were washed once with ice-cold PBSB and incubated with 1000x dilution of mouse anti-Myc and chicken anti-His (Invitrogen, PA1-9531) antibodies on ice for 20 min ...
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bioRxiv - Molecular Biology 2021Quote: ... fluorescence-labeled HIV-1JR-FL Env(-) carrying the (His)6 epitope tag was incubated with biotin-conjugated anti-(His)6 tag antibody (HIS.H8, Invitrogen) at 4° for two hours.
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bioRxiv - Immunology 2021Quote: ... RBD-His and Spike-his containing supernatants were batch purified using the HisPur™ Ni-NTA Resin (ThermoFisher Scientific). Supernatants were incubated with 6 ml of resin for 1h at RT ...
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bioRxiv - Immunology 2019Quote: ... in OT-1 culture medium supplemented with 100 U/ml mouse recombinant IL-2 (Thermo Fisher Scientific, catalog 14802164) in a 37 °C/5% CO2 incubator ...
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bioRxiv - Developmental Biology 2021Quote: ... re-suspended in PBS containing a viability marker (7-AAD – 7-Aminoactinomycin, Invitrogen – A1310), filtered again through 70-µm cell strainers and incubated on ice for 5 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... 7 and 7 fish respectively and dissociated in 0.25% Trypsin-EDTA (Gibco, ThermoFisher Scientific) and 8 mg/ml Collagenase from Clostridum histolyticum (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2022Quote: ... 7 and 7 fish respectively and dissociated in 0.25% Trypsin-EDTA (Gibco, ThermoFisher Scientific) and 8 mg/ml Collagenase from Clostridum histolyticum (Sigma-Aldrich ...
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bioRxiv - Synthetic Biology 2023Quote: ... The samples were incubated overnight with Paired Box 7 (PAX 7) antibodies (Invitrogen, USA) in blocking solution at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... wells were treated with Caspase-3/7 (CellEvent™ Caspase-3/7 Green, Invitrogen) 1:1000 in treatment media ...
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bioRxiv - Neuroscience 2022Quote: ... high dose IL-6 stimulation received 100 ng/ml IL-6 (Gibco; PHC0066) or 100 pM acetic acid vehicle stimulation for either 3-or 24-hours and immediately collected for analysis ...
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bioRxiv - Immunology 2023Quote: ... IL-10 and IL-17 were tested using cytokine measuring kits (Invitrogen, USA) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... IL-1β and IL-1α signal was amplified with Tyramide SuperBoostTM (Thermo Fisher) using biotinylated horse–anti-goat IgG (7.5 µg/mL ...
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bioRxiv - Genetics 2019Quote: ... Day 6–7: DMEM (Gibco) with 25 mM glucose containing 1:100 B27 (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... and 7-AAD (Thermofisher Scientific) for 30min ...
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bioRxiv - Developmental Biology 2021Quote: ... 7 (Applied Biosystems, Zug, Switzerland).
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bioRxiv - Cancer Biology 2021Quote: ... 7-AAD (ThermoFisher Scientific, #A1310), AnnexinV-PE (BioLegend ...
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bioRxiv - Cancer Biology 2022Quote: ... 7’-Dichlorofluorescin diacetate (DCFDA; Invitrogen). DCFDA is de-esterified into its fluorescent form after action of intracellular esterases and oxidation by reactive oxygen species within the cell (Ishii et al. ...
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bioRxiv - Microbiology 2021Quote: ... MgCl2 (ThermoFisher AM9530G; 7 mM), and SmartScribe Reverse Transcriptase (TaKaRa 639538 ...
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bioRxiv - Neuroscience 2021Quote: ViiA 7 system (Applied Biosystems)
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bioRxiv - Cell Biology 2021Quote: ... 7’ dichlorofluorescein diacetate (DCFDA, Invitrogen at 37°C for 30 min ...
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bioRxiv - Developmental Biology 2020Quote: Cos-7 cells (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... 7-AAD (Thermo Fisher Scientific) and Annexin-V PE (BD ...
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bioRxiv - Biophysics 2022Quote: ... 7 kDa MWCO (Thermo Scientific). Terminase protomer was exchanged into a 200 mM ammonium acetate salt ...
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bioRxiv - Developmental Biology 2023Quote: ... using 7-AAD (ThermoFisher Scientific) as a dead stain.
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bioRxiv - Developmental Biology 2023Quote: ... using 7-AAD (ThermoFisher Scientific) as a dead cell stain ...
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bioRxiv - Bioengineering 2024Quote: ... 7 kDa MWCO (Thermo Scientific). The extent of biotinylation was measured using the QuantTag Biotin Quantification kit (Vector Laboratories ...
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bioRxiv - Neuroscience 2022Quote: ... 350ng of pCAGIG-VENUS was used to transfect the neurons two days after adding mouse glia (PNI day 7) using Lipofectamine 2000 (ThermoFisher). A full media change using conditioned media was performed 5-6 hours post-transfection ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then washed and stained for 20 min at 4 °C with the following antibodies: anti-mouse CD8α APC-efluo780 (clone 53-6-7, ebioscience/ Thermofisher), anti-mouse CD8β AF700 or BUV495 (clone YTS156 ...
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bioRxiv - Cell Biology 2020Quote: ... Stable transformants of ZP161 expressing rat Pex14 variants tagged with N-terminal hexahistidine (His-Pex14) were isolated by transfection of pcDNAZeo-D/His-RnPEX14 variants (see below) followed by selection with Zeocin (Invitrogen), as described (Okumoto et al. ...
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Lactic Acid Containing Polymers Produced in Engineered Sinorhizobium meliloti and Pseudomonas putidabioRxiv - Microbiology 2019Quote: ... the gel was used for His-tag staining following the InVision(tm) His-tag In-Gel Stain protocol provided by Invitrogen.
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bioRxiv - Immunology 2022Quote: ... pAc5.1A/V5-His-CBF and pAc5.1A/V5-His-EF-1α were transfected into S2 cells using Cellfectin II Reagent (Invitrogen, USA), respectively ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane was probed with primary antibodies including anti-tau mouse ET3 (1:1000 dilution) and 6X - His Tag rabbit monoclonal antibody (Invitrogen, 1:3500 dilution). The secondary antibodies used were ...
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bioRxiv - Cell Biology 2023Quote: Primary antibodies used in western blotting (WB) and immunofluorescence (IF) were anti-6x His mouse monoclonal (Invitrogen, #MA1-21315, 1:5000 in WB), anti-GST mouse monoclonal (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... His-tags were removed with AcTEV protease (Invitrogen) overnight and proteins further purified with a HiTrap SP HP cation exchange column (GE Healthcare).
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bioRxiv - Molecular Biology 2020Quote: A pcDNA3.1/V5-His TOPO expression vector (Invitrogen) encoding the protein-coding sequence of human SM (NM_003129.4 ...
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bioRxiv - Biochemistry 2020Quote: ... into pT7-N-His-GST (Thermo Fisher Scientific) or pET28a (EMD Millipore) ...
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bioRxiv - Biophysics 2021Quote: ... supplemented with 10% HI-FBS (16140-071, Gibco), 100 U/mL Penicillin-Streptomycin (15140122 ...
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bioRxiv - Biochemistry 2021Quote: ... then cloned into pIB/V5-His vector (Invitrogen), fused with V5 epitope at the C-terminus ...
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bioRxiv - Biophysics 2020Quote: ... 10 % v/v Hi-Di formamide (Thermo Scientific), supplemented with 2 mM vanadyl ribonucleoside complex) ...