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Citations for Thermo Fisher :
551 - 600 of 10000+ citations for IL 1beta Mouse CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... plasmids encoding targeted proteins were transiently transfected into ExpiCHO-S cells by using ExpiFectamine™ CHO transfection kit (Thermo Scientific, A29129). Transfected cells were cultured in stackable CO2 incubator shaker (Kühner AG ...
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bioRxiv - Microbiology 2021Quote: ... 100 µg of plasmid and 320 μl of ExpiFectamineTM CHO reagent were mixed in 7.7 ml of cold OptiPRO™ medium (Thermo Fisher). After the first feed on day 1 ...
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bioRxiv - Bioengineering 2021Quote: CHO cells and CHO EVs for SEM were fixed in 4% EM grade glutaraldehyde overnight on poly-L-lysine coated glass coverslips (Fisher Scientific). Samples were processed for SEM imaging by treating the samples to 1% osmium tetroxide for 1 hour ...
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bioRxiv - Biochemistry 2021Quote: ... CHO cells stably expressing LI-cadherin-GFP were established using Flp-In™-CHO Cell Line following the manufacturer’s protocol (ThermoFisher Scientific). Cloning was performed by the limiting dilution-culture method ...
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bioRxiv - Microbiology 2019Quote: ... 100 μg of plasmid and 320 μl of ExpiFectamineTM CHO reagent were mixed in 7.7 ml of cold OptiPRO™ medium (Thermo Fisher). After the first feed on day one ...
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bioRxiv - Biochemistry 2021Quote: ... One ampule of cells from a working cell bank was thawed in CD-CHO basal medium (Thermo Fisher Scientific, Waltham, MA), along with 0.5 % anti-clumping agent (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... ExpiFectamine CHO Enhancer (6 μl per ml of culture) and ExpiCHO Feed (240 μl per ml of culture) (Thermo Fisher Scientific) were added 18-22 hours post-transfection and the temperature was decreased to 33 °C ...
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bioRxiv - Microbiology 2019Quote: ... EDE2 B7 (Dejnirattisai et al., 2015), CR4354 (Kaufmann et al., 2010) were expressed by transient transfection of Expi-CHO-S cells (Cat# A29129; ThermoFisher Scientific). Variable heavy and light chain sequences of the above control mAbs used for gene synthesis and cloning into expression vectors were based on PDB IDs 4UT9 ...
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bioRxiv - Microbiology 2021Quote: ... 100 μg of plasmid and 320 μl of ExpiFectamine CHO reagent were mixed in 7.7 ml of cold OptiPRO™ medium (Thermo Fisher). After the first feed on day one ...
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bioRxiv - Microbiology 2022Quote: ... The CHO cell growth and Spike recombinant plasmid transfection were performed according to the ExpiCHO expression system User Guide (MAN0014337, ThermoFisher website). The S-trimer expression was under the control of a strong CMV promoter ...
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bioRxiv - Microbiology 2022Quote: ... 100 μg of antigen plasmid and 320 μl of ExpiFectamineTM CHO reagent were mixed in 7.7 ml of cold OptiPRO™ medium (Thermo Fisher). After the first feed on day 1 ...
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bioRxiv - Cell Biology 2022Quote: ... Transfections of NIH 3T3 and CHO cells were carried out using a Neon MP-100 microporator system (Life Technologies, Carlsbad, CA). Transfection of HEK293 cells was performed using the calcium phosphate method ...
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bioRxiv - Neuroscience 2023Quote: Chinese hamster ovary (CHO) cells were maintained in Dulbecco’s Modified Eagle Medium: Nutrient Mixture F-12 (Thermo Fisher Scientific, Waltham, MA) supplemented with 10% (v/v ...
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bioRxiv - Immunology 2023Quote: ... trimer was produced and differentially purified from a CZA97.012 SOSIP.664-expressing CHO-cell line maintained in ExpiCHO Expression Medium (Thermo Fisher Scientific) (82) ...
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bioRxiv - Microbiology 2023Quote: ... 100 μg of antigen plasmid and 320 μl of ExpiFectamineTM CHO reagent were mixed in 7.7 ml of cold OptiPRO™ medium (Thermo Fisher). After the first feed on day 1 ...
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bioRxiv - Cell Biology 2022Quote: ... and GlyA-CHO cells (glycine auxotrophic for growth and a gift from Dr. P. J. Stover, Cornell University) using Lipofectamine 2000 (Invitrogen # 11668019). Briefly ...
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bioRxiv - Genomics 2023Quote: A mAb producing CHOK1 cell line (CHO K1 mAb) was seeded at a density of 2 × 105 cells/ml in 50ml SFM-II media (Gibco, 12052098) in 8 replicate shake flasks in a Kuhner orbital shaker at 170rpm at 5% CO2 ...
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bioRxiv - Immunology 2022Quote: The pcDNA3.1 plasmids encoding S and S-Fc were transfected into CHO cells using PEI MAX 40000 (Fisher Scientific, Cat# NC1038561). Stable cell lines were selected and maintained under G418 (1 mg/ml) ...
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bioRxiv - Microbiology 2023Quote: Human lung cancer A549 cells and Chinese hamster ovary (CHO) cells were cultured in DMEM medium (GIBCO, Thermo Fisher Scientific, China) and RPMI1640 medium (GIBCO ...
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bioRxiv - Microbiology 2023Quote: Human lung cancer A549 cells and Chinese hamster ovary (CHO) cells were cultured in DMEM medium (GIBCO, Thermo Fisher Scientific, China) and RPMI1640 medium (GIBCO ...
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bioRxiv - Immunology 2024Quote: ... and soluble membrane protein (SMP) preps were extracted from Chinese hamster ovary (CHO) cells and were biotinylated using an NHS-LC-Biotin kit (ThermoFisher Scientific). Yeast displaying IgGs on their surface were incubated with biotinylated SCP and SMP preps at a 1:10 dilution in PBSF (PBS with 0.1% w/v BSA ...
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bioRxiv - Microbiology 2024Quote: ... 25 μg F antigen plasmid and 80 μl of ExpiFectamineTM CHO reagent were mixed in 1.9 ml of cold OptiPRO™ medium (Thermo Fisher). After the first feed on day 1 ...
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bioRxiv - Biochemistry 2024Quote: ... ExpiCHO-S cells were grown to a density of 6 x 106 cells/mL and transfected using the ExpiFectamine CHO Transfection Kit (ThermoFisher Scientific) and expression carried out for 7-10 days post-transfection at 37°C with 8% CO2 ...
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bioRxiv - Cancer Biology 2021Quote: ... in CD-1 mouse, Sprague−Dawley rat, and human plasma (Bioreclamation, Inc., Hicksville, NY) by equilibrium dialysis using the RED Device (Thermo Fisher Scientific, Rockford, IL). Compounds were added to pooled plasma (n ≥ 3 ...
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bioRxiv - Immunology 2022Quote: ... Murine IFNγ levels and IL-2 levels were measured using the Mouse Interferon gamma (IFNγ) Uncoated ELISA Kit (Thermo Fisher Scientific, 88-7314-76) or IL-2 Mouse Uncoated ELISA Kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated at room temperature for 1 h with secondary antibodies Alexa Fluor 568 goat anti-mouse IgG and Alexa Fluor 488 goat anti-rabbit IgG (1:500; Invitrogen Life Technologies, Rockford, IL, USA), counterstained with Hoechst 33258 (1:1,500 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Supernatant obtained from the BALF was used to measure the endogenous release of IL-6 using a commercially available Murine IL-6 ELISA kit according to the manufacturer’s instructions (ThermoFisher, InVItrogen). The absorbance was read at 450 nm and secreted IL-6 was analyzed using Gen5 data analysis software (BioTek Instruments ...
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bioRxiv - Immunology 2019Quote: Serum IL-6 and IL-17A concentrations were assessed by ELISA according to the manufacturer’s instructions (Thermo Fisher Scientific).
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bioRxiv - Immunology 2023Quote: ... orca-pro-(h)IL-1α and pro-IL-1β genes were synthesized and cloned to pcDNA3.1(+) vectors (Life Technologies). pro-α-mat-β chimeric expressed the Met1to Arg112 of human IL-1α ...
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bioRxiv - Biochemistry 2023Quote: ... The level of IL-8 in cell culture supernatants was determined using a human IL-8 ELISA kit (Invitrogen).
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bioRxiv - Immunology 2021Quote: ... The plasmids were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The plasmids were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2019Quote: ... Batch cultivation was performed in 250 mL shake flasks containing 60-80 mL CD-CHO medium (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 8 mM L-glutamine (Life Technologies ...
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bioRxiv - Immunology 2021Quote: ... microscale transfection were performed (∼1 ml per antibody) of CHO cell cultures using the Gibco ExpiCHO Expression System and a protocol for deep 96-well blocks (Thermo Fisher Scientific). In brief ...
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bioRxiv - Immunology 2021Quote: ... were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... The plasmids were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: Expression SΔTM trimers was performed by transfection of ExpiCHO-S™ cells with the plasmids described above using the ExpiFectamine™ CHO transfection kit (Gibco™ ...
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bioRxiv - Immunology 2021Quote: ... 100 μg of plasmid and 320 μl of ExpiFectamine™ CHO reagent were mixed in 7.7 ml of cold OptiPRO™ medium (Thermo Fisher). After the first feed on day 1 ...
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Sterilizing immunity against SARS-CoV-2 in hamsters conferred by a novel recombinant subunit vaccinebioRxiv - Microbiology 2020Quote: ... The plasmid expression plasmid carrying each SARS-CoV-2-associated protein was transiently transfected into ExpiCHO-S cells by using the ExpiFectamine™ CHO transfection kit according to the manufacturer’s instruction (Thermo Scientific, A29129). After transfection ...
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bioRxiv - Immunology 2021Quote: ... and the corresponding light chain (human IgKappa, IgLambda) expression vectors respectively and produced in transiently expressed in Expi-CHO-S cells (Thermo Fisher, #A29133) at 37°C and 8% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... IgG or Fab were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... GenBank accession number NM_004519) was generated using Flp-In™-CHO cells according to the supplier’s protocol (Thermo Fisher Scientific, Waltham, USA). CHO-Q3 stable cells were maintained under dual selection with Zeocin (100 μg/ml ...
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bioRxiv - Bioengineering 2021Quote: ... To identify these target cells during downstream analysis CHO DP-12 cells were first stained with CellTracker™ Blue CMAC Dye (Thermo Fisher). 85 µm nanovials containing RGD and cells were seeded into a 12-well plate as described above and then incubated at 37°C for 2 hours to allow cells to adhere to the particles ...
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bioRxiv - Immunology 2020Quote: ... and soluble cytosolic protein (SCP) fractions were extracted from Chinese hamster ovary (CHO) cells and biotinylated using NHS-LC-Biotin (Thermo Fisher Scientific) reagent ...
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bioRxiv - Immunology 2021Quote: ... The plasmids were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The plasmids were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The plasmids were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Expression plasmids encoding the heavy and light chains of the COVA1-16 Fab were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (HC:LC) using ExpiFectamine™ CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... microscale transfection was performed (∼1 mL per antibody) of CHO cell cultures using the Gibco ExpiCHO Expression System and a protocol for deep 96-well blocks (Thermo Fisher Scientific). In brief ...
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bioRxiv - Immunology 2020Quote: ... microscale transfection were performed (~1 ml per antibody) of CHO cell cultures using the Gibco ExpiCHO Expression System and a protocol for deep 96-well blocks (Thermo Fisher Scientific). In brief ...