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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for IL 1beta Mouse CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... IL-17A (Invitrogen #88-7371-88), or IL-5 (BD Biosciences #51-1805KZ ...
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bioRxiv - Immunology 2023Quote: ... IL-13 PE/Cy7 (eBio13A, Invitrogen), IL-5 APC (TRFK5 ...
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bioRxiv - Neuroscience 2023Quote: ... Enhanced chemiluminescence (Thermo Scientific, IL, USA) was used to see the bands ...
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bioRxiv - Immunology 2023Quote: ... IL-10 (JES5-16E3, APC, Invitrogen), and GM-CSF (MP1-22E9 ...
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bioRxiv - Cell Biology 2023Quote: ... DAB Substrate (Thermo Scientific, Rockford, IL) was used to achieve peroxidase reaction followed by hematoxylin (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... and IL-10 (ProcartaPlex, Thermo Fisher) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... anti-IL-4-PB (Thermo Fisher Scientific Cat# 48-0042-82 ...
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bioRxiv - Immunology 2024Quote: ... and anti-IL-2-PE (Affymetrix eBioscience ...
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bioRxiv - Immunology 2024Quote: ... human IL-8 (Invitrogen™ 88808677), human IL-1beta (Invitrogen™ 88726177 ...
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bioRxiv - Neuroscience 2020Quote: ... Next the cells were washed with tris buffer with 0.1% triton X and were incubated with secondary antibody goat anti mouse Cy3 (1:500, Invitrogen, Rockland, IL, USA) for Iba-1 and anti-GFAP (1:500 ...
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bioRxiv - Physiology 2021Quote: The concentrations of IL-1β in tissue extracts of ovaries and livers were determined using an IL-1 beta Pro-form Mouse Uncoated ELISA kit (88-8014-22; Thermo Fisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Alexa Fluor 488 conjugated goat anti-rabbit and Alexa Fluor 647 conjugated goat anti-mouse (Thermo Fisher, Rockford, IL, USA, 1:300).
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bioRxiv - Cell Biology 2022Quote: ... Alexa Fluor 488 donkey anti-mouse IgG and Alexa Fluor 647 donkey-rat IgG (1:1,000; Thermo Fisher Scientific, Rockford, IL, USA). Liver ...
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bioRxiv - Immunology 2022Quote: ... Purified molecules or mouse anti-human IL-15 antibodies were coated on a MaxiSorp™ 96-well flat-bottom ELISA plate (ThermoFisher Scientific) for 12h and for 72h ...
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bioRxiv - Immunology 2023Quote: ... Intracellular cytokines were stained at 4°C overnight with FITC or PerCP-e710-conjugated rat anti-mouse IL-1β (NJTEN3; Thermo Fisher Scientific) and PE-conjugated Armenian hamster anti-mouse IL-1α (ALF-161 ...
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bioRxiv - Immunology 2024Quote: Individual cytokines in supernatants from cell culture experiments were measured using the species-appropriate ELISA Kits mouse IL-6 (Invitrogen™ 88706488), mouse IL-1beta (Invitrogen™ 88701388) ...
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bioRxiv - Cell Biology 2020Quote: ... Released IL-1β was quantified using the Human IL-1 beta ELISA Ready-Set-Go! Kit (ThermoFisher) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... previously cultured for 24h with IL-15/IL-15R complex recombinant protein (100ng/ml, Thermo Fisher Scientific) and retinoic acid (100ng/ml) ...
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Incidence of an intracellular multiplication niche amongst Acinetobacter baumannii clinical isolatesbioRxiv - Microbiology 2021Quote: ... The concentration of IL-6 was quantified by ELISA (Human IL-6 ELISA Ready-SET-Go!, Thermofisher) by following the supplier’s protocol.
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bioRxiv - Microbiology 2023Quote: ... the supernatants were collected and we measured the IL-1β concentrations using IL-1β ELISA kits (Invitrogen) and following manufacturer instructions.
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bioRxiv - Biophysics 2021Quote: A stable β2AR-overexpressing cell line was produced by transfecting CHO-K1 cells (ECACC) with a plasmid coding for the human β2AR with C-terminal GFP using lipofectamine 2000 (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... cells stably expressing I-Ek-MCC (CHO-I-Ek) were maintained by passage every 3 days in complete RPMI 1640 medium (ThermoFisher Scientific ...
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bioRxiv - Genetics 2019Quote: ... ExpiCHO-S cells were transfected with full-length pRTL1-GCSF vectors using the ExpiFectamine™ CHO Transfection Kit (Life Technologies Corporation ...
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bioRxiv - Genetics 2021Quote: ... The hGOT1-GFP and mutant hGOT1-GFP constructs were transfected into Chinese Hamster Ovary (CHO) (ExpiCHO-S) cells with an ExpiFectamine Transfection Kit (Gibco) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... were transfected with the appropriate light chain and heavy chain encoding plasmids for each Fab following the ExpiFectamine CHO Transfection Kit (ThermoFisher) high titer protocol ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids containing the Fab heavy chains and light chains were chemically co-transfected into an afucosylated ExpiCHO cell line using the ExpiFectamine CHO Transfection Kit (Gibco). After 9 days of transient expression ...
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bioRxiv - Biophysics 2022Quote: ... were transfected with the appropriate light chain and heavy chain encoding plasmids for each Fab following the ExpiFectamine CHO Transfection Kit (ThermoFisher) high titer protocol ...
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bioRxiv - Biochemistry 2021Quote: The wild-type (WT) and mutant GLP-1R constructs were integrated into FlpIn-CHO cells using FlpIn Gateway technology system (Invitrogen). Stable CHOFlpIn expression cell lines were selected using 600 μg/ml hygromyocin B ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Stable Flp-In-CHO cell lines were generated through co-transfection of the pcDNA5/FRT expression vector (Thermo Fisher Scientific) containing the gene of interest and the Flp recombinase expressing plasmid ...
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bioRxiv - Microbiology 2021Quote: Plasmid pCAG-CD5-S-ecto-6P-Spytag was transiently transfected into ExpiCHO cells using ExpiFectamine CHO transfection kit (Thermo Fisher). After 18-22 hr of transfection ...
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bioRxiv - Cell Biology 2022Quote: Histidine-tagged Mindin was purified from conditioned media collected from CHO-Mindin cells using Ni-NTA beads (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: ... were transfected into HEK293T cells (3×105/well) using PEI or into CHO cells (1.5×105/well) with lipofectamine 3000 reagent (ThermoFisher, L3000001) in 24-well plates ...
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bioRxiv - Microbiology 2020Quote: ... HEK293 and CHO cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12; Gibco, #11320033), supplemented with 10% (w/v ...
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bioRxiv - Microbiology 2021Quote: CHO cells stably expressing SARS-CoV-2 S-glycoprotein were seeded in 96 well plates for microscopy (Thermo Fisher Scientific) at 12’500 cells/well and the following day ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: CHO cell lines overexpressing FPR2 and FPR1 receptors were generated in-house and propagated in Ham’s F12 medium (Gibco Biosciences) supplemented with 10% (v/v ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Total RNA from CHO-FPR2 overexpressing cells or HL-60 cells was isolated using Trizol reagent (Invitrogen, Carlsbad, CA, USA) and purified using RNeasy Mini kits (Qiagen ...
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bioRxiv - Immunology 2020Quote: ... were transfected with the plasmid expressing S-2P protein ectodomains by ExpiFectamine 293 transfection kit and ExpiFectamine CHO transfection kit (ThermoFisher), respectively ...
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bioRxiv - Biophysics 2022Quote: ... and a combination of the glCV30 Hc and glCV30 Lc vectors or a combination of the glCV30-SDF-1α Hc and glCV30 Lc vectors were introduced into the CHO-S cells (Gibco). The transfected cells were grown in the ExpiCHO expression medium (Gibco ...
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bioRxiv - Physiology 2023Quote: Myc-tagged wild-type (WT) and PKA-resistant mutant (Ser791Ala) Raptor vectors7 were transfected into CHO-GLP-1R cells using lipofectamine-3000 (Invitrogen). 48h later ...
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bioRxiv - Immunology 2022Quote: ... expansion and production of the CHO cells from the working cell bank (WCB) into shake flasks and bioreactor (Applikon) using serum-free medium (Gibco). At the end of the growth phase ...
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bioRxiv - Microbiology 2023Quote: ... CHO-DSP1 and MeWo-DSP2 cells were kept under puromycin selection (8 µg/ml for CHO-DSP1 and 5 µg/ml for MeWo-DSP2; Gibco). BHK-21 cells were grown in GMEM supplemented with 20 mM Hepes pH 7.4 ...
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bioRxiv - Immunology 2023Quote: ... CHO and G8 hybridomas cells were loaded respectively with 10 μM of the red cell-labeling agent DDAO (Molecular Probes) [ex/em 635/662] and 1 μM Vybrant CFDA SE [ex/em 492/517] in serum-free RPMI-1640 for 30 minutes at 37°C ...
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bioRxiv - Neuroscience 2024Quote: Chinese Hamster Ovary (CHO) cells were maintained similar to HEK cells with the following differences: Culture media was IMDM (Invitrogen) supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2021Quote: ... for 5 min each and then incubated with 1:200 diluted anti-rabbit or mouse IgG-horseradish peroxidise (HRP) (Thermo Scientific, Rockford, IL) at room temperature for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... the membranes were washed three times in 1X TBST and incubated with horseradish peroxidase-conjugated goat-anti-mouse or -rabbit secondary antibodies for one hour at room temperature (1:10,000, Invitrogen #31430, #31460, Rockford, IL). Membranes were subsequently washed three times in TBST ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Interleukin 6 (IL-6) was measured using an IL-6 ELISA kit following manufacturer’s instruction (Invitrogen, Catalog # CHC1263). Similarly ...
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bioRxiv - Microbiology 2022Quote: ... supernatants were collected for cytokine analysis by ELISA (IL-2 BD-OptEIA™; IL-17A, IFN-γ ThermoFisher) following manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... was made by mixing 2.5ug of IL-2 monoclonal antibody (JES6-1A12, 2B Scientific) with 7.5ug recombinant IL-2 (ThermoFisher) in 200ul of PBS for 20 minutes at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... IL-1β secretion from PMA-THP1 was detected using IL-1 beta Human ELISA kit (#KHC0011, ThermoFisher Scientific) and IL-1 beta Human ELISA kit ...
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bioRxiv - Immunology 2023Quote: ... IL-18 and human IL-1β was analyzed in the cell culture supernatant by commercial ELISA kits (Invitrogen) following the manufacturer’s recommended procedures.