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Citations for Thermo Fisher :
351 - 400 of 8268 citations for IL 17RA Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... and HEK293 or RPMI 1640 (ATCC modification) (ThermoFisher, A1049101) (for Jurkat cells complete with 5% pen/strep and 10% FBS ...
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bioRxiv - Biochemistry 2023Quote: ... HEK293 cells were grown in RPMI 1640 medium (Gibco) supplemented with 10% FBS ...
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bioRxiv - Immunology 2023Quote: ... HEK293 cells were transfected using lipofectamine 2000 (Thermofisher, USA) following the manufacturer protocol ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293 cells were propagated in MEM with GlutaMAXTM (Gibco) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293-FT (RRID: CVCL_6911) was purchased from Thermo Fisher Scientific (#R70007) ...
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bioRxiv - Immunology 2021Quote: ... and the ELISPOT plate was incubated for 4 hours at 4°C with biotinylated goat anti-human IgG Fc (Invitrogen), followed by incubation for 1 hour at room temperature with horseradish peroxidase (HRP)–conjugated avidin (Vector Laboratories) ...
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bioRxiv - Molecular Biology 2020Quote: Mammalian expression plasmids encoding SARS VHH72 and MERS VHH-55 (73) with a C-terminal a monomeric human Fc tag were transfected into ExpiCHO cells (Thermofisher) using ExpiFectamine as manufacterer’s recommendation ...
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bioRxiv - Developmental Biology 2022Quote: ... These zona-free eggs were incubated with 200 nM Fc-fusion proteins in Biggers-Whitten-Whittingham (BWW) medium for 1 h and then stained with goat anti-human IgG Fc antibody DyLight 488 (Invitrogen) at a dilution of 1:50 for 1 h at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... Antibody binding was detected using goat anti-Human IgG Fc Secondary Antibody conjugated to horse radish peroxidase (Thermo Fisher Scientific), diluted 1:5000 in blocking solution and incubated for 1 hr at RT ...
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bioRxiv - Microbiology 2022Quote: ... and then incubated with a 1:10000 dilution (in PBST with 1% BSA) of goat anti-human Fc HRP-conjugated antibody (Invitrogen) at room temperature for 1 hour ...
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bioRxiv - Molecular Biology 2021Quote: ... and S309 and VHH72-Fc or variants thereof was detected with an AF633 conjugated goat anti-human IgG antibody (Invitrogen). Binding of monomeric VHHs to SARS-CoV-1 or SARS-CoV-2 S was detected with a mouse anti-HisTag antibody (AbD Serotec ...
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bioRxiv - Neuroscience 2020Quote: ... β-neurexin-1 extracellular domain was cloned into the pCAGGS vector with a C-terminal human Fc tag and the ATD regions of GluA1–4 were cloned into a modified pDisplay vector (Invitrogen), as described previously (9) ...
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bioRxiv - Biochemistry 2021Quote: ... The slide was washed thrice with PBS-T for 1 minute and further incubated with goat anti-human IgG (Fc)-Cy5 (Invitrogen) antibodies at 1:2500 dilution ...
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bioRxiv - Bioengineering 2019Quote: ... was diluted 1:200 in PBS/5% FCS/0.1% Tween® 20 and primary MMP1 antibody (mouse anti-human; Invitrogen Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The cells were then washed and incubated with PE-conjugated goat anti-human Fc antibodies (Thermo Fisher 12-4998-82) for 30 min at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... and PToV Hemagglutinin Esterase non-binding (F271A) (1176bp) fused to human IgG-Fc followed by a 6xHis tag in the Insect expression vector pFastBac1 (ThermoFisher) was kindly provided by Colin Parrish (PToV-HE_S46A-Fc_Hisx6/pFastBac1 ...
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bioRxiv - Bioengineering 2023Quote: ... The membranes were then incubated with a 1:10,000 dilution of goat anti-human Fc HRP secondary antibody (Invitrogen, A18817) in PBST for 1 h ...
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bioRxiv - Biochemistry 2023Quote: ... Plates were slapped dry and washed 4X in TBST followed by addition of 50 μL 1:5000 Goat anti-Human IgG Fc Secondary Antibody with HRP (Invitrogen) for one hour at 37°C ...
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bioRxiv - Immunology 2023Quote: ... The wells were washed 5x and incubated with 100 µl/well with an HRP-conjugated anti-human IgG Fc antibody (1:10000 dilution, Invitrogen) for 1 hour ...
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Macropinocytosis mediates resistance to loss of glutamine transport in triple-negative breast cancerbioRxiv - Cancer Biology 2024Quote: ... incubated with primary antibody (anti-human ASCT2 from MedImmune diluted 1:3000 in FACS buffer) for 30 min and secondary antibody for 20 min (Goat anti-human IgG Fc PE secondary antibody (Invitrogen) diluted 1:250 in FACS buffer) ...
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bioRxiv - Immunology 2023Quote: ASCs (FVS780-CD20-CD3-CD14-CD16-CD19+CD27hiCD38hi) were sort-purified from human peripheral blood mononuclear cells into culture media (10% FCS, 2 mM L-glutamine (Gibco), 100 units/mL Penicillin and 100 ng/mL Streptomycin (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... plus 10 % FCS (Gibco, Thermo Fisher ...
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bioRxiv - Developmental Biology 2021Quote: ... and 1% FCS (Gibco). Followed by 3 days of culture ...
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bioRxiv - Bioengineering 2019Quote: ... FC from Life Technologies; and 2 ...
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bioRxiv - Neuroscience 2020Quote: ... containing 10% FCS (Invitrogen), and gentamycin (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... dialyzed FCS (Thermofisher, # 26400044), 28 μg/mL L-arginine-HCl (Arg0 or Arg10 ...
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bioRxiv - Biochemistry 2021Quote: ... including 10% FCS (Gibco), 2 mM glutamine (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2021Quote: ... with 10 % FCS (Gibco), 2 mM glutamine (Gibco) ...
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bioRxiv - Microbiology 2022Quote: ... containing 10 % FCS (Gibco). Macrophages were seeded into 24-well plates at 105 cells per well.
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bioRxiv - Microbiology 2022Quote: ... containing 10% FCS (Gibco), 40 mM Hepes (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... and 0.5% FCS (Gibco) in PBS with 0.01% Tween-20 (Sigma-Aldrich) ...
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bioRxiv - Immunology 2020Quote: ... + 10% HI-FCS (Gibco) + 1% Peniciliin/Streptomycin (100x ...
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bioRxiv - Immunology 2021Quote: ... 10% FCS (Gibco, 26400044), 2.5% 1M HEPES buffer (Sigma ...
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The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... containing 10% FCS (Gibco), 1 mg/ml Collagenase VIII and 50 µg/ml DNase I (both Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... and 10% FCS (Gibco). MCF-7 human breast cancer cells were obtained from ATCC and cultured in Roswell Park Memorial Institute medium (RPMI-1640 ...
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bioRxiv - Cancer Biology 2022Quote: ... and 10% FCS (Invitrogen).
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bioRxiv - Cell Biology 2022Quote: ... and 10% FCS (GIBCO). Cells were regularly tested for mycoplasma contamination.
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bioRxiv - Immunology 2022Quote: ... containing 10% FCS (Gibco) and 1% Penicillin/Streptomycin (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2022Quote: ... with 5% FCS (Gibco), MEM only ...
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bioRxiv - Genetics 2023Quote: ... with 15% FCS (ThermoFisher), 0.1 mM beta-mercaptoethanol,100 U/ml penicillin and 100 mg/ml streptomycin (Gibco) ...
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bioRxiv - Cell Biology 2023Quote: ... and 10% FCS (Gibco) for 10 days ...
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bioRxiv - Cell Biology 2023Quote: ... 10% FCS (Invitrogen, 41966029), 10% penicillin/streptomycin at 37°C in 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... with 10 % FCS (Gibco) and 1 % PenStrep ...
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bioRxiv - Cell Biology 2023Quote: Interleukin-6 levels in supernatants were determined using commercially available human IL-6 ELISA kits according to the manufacturer’s protocol (Invitrogen). A detailed description of the procedure are reported in Supporting methods ...
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bioRxiv - Microbiology 2019Quote: ... Plates were washed 6 times and incubated with horseradish peroxidase (HRP)-conjugated mouse anti-human IgG Fc secondary antibody (Cat# 05-4220; ThermoFisher Scientific) diluted 1:1000 in 100 µl blocking buffer for 1 h at room temperature or 37°C ...
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bioRxiv - Immunology 2021Quote: ... residues 306–577 of MERS-CoV S with a C-terminal monomeric human IgG Fc-tag and an 8x HisTag (SARS-CoV RBD-SD1) were transiently transfected into FreeStyle293F cells (Thermo Fisher) using polyethylenimine ...
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bioRxiv - Biochemistry 2021Quote: ... Detection was enabled by addition of 100 µL Goat anti-Human IgG Fc secondary antibody conjugated to horseradish peroxidase (ThermoFisher #A18817), diluted 1:50,000 into PBSM and incubated in the above plate shaker for 1 h at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Serum samples at varying dilutions were incubated for 2 h followed by detection by a goat anti-human Fc-HRP secondary antibody (ThermoFisher Scientific). The plasma concentration of AvFc was calculated by interpolating from a standard curve ...
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bioRxiv - Genomics 2022Quote: ... Microarrays were then washed (three times with PBS-T for 1 minute) and peptide binding was detected with isotype-specific secondary goat anti-human IgG (Fc) DyLight680 (ThermoFisher Scientific) and goat anti-human IgA (alpha chain ...
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bioRxiv - Immunology 2020Quote: ... or residues 319–591 of SARS-CoV-2 S with a C-terminal monomeric human IgG Fc-tag and an 8x HisTag (SARS-CoV-2 RBD-SD1) were transiently transfected into FreeStyle293F cells (Thermo Fisher) using polyethylenimine ...