Labshake search
Citations for Thermo Fisher :
201 - 250 of 8268 citations for IL 17RA Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... the Expi-HEK293 cells (Thermo Scientific) were transfected with the mammalian expression plasmid pHLSec containing the recodonized SUB1 ...
-
bioRxiv - Molecular Biology 2022Quote: HEK293 TREx cells (Invitrogen Canada Inc.) were cultured in high-glucose Dulbecco’s modified Eagle’s medium (DMEM ...
-
bioRxiv - Microbiology 2023Quote: ... and HEK293-S cells (ThermoFisher Scientific) were cultured in FreeStyle293 expression medium (Life Technologies) ...
-
bioRxiv - Immunology 2023Quote: ... FreeStyle™ HEK293-F cells (Gibco) were co-transfected with two plasmids ...
-
bioRxiv - Biochemistry 2019Quote: ... The cells were then incubated using FITC-labelled goat-anti-human IgG-FC antibody (ThermoFisher Scientific) (200 ng/ul ...
-
bioRxiv - Immunology 2019Quote: ... cells were stained with a PE-conjugated anti-human IgG1(Fc) secondary antibody (1:50) (ThermoFisher) for additional 15min ...
-
bioRxiv - Microbiology 2021Quote: ... R-phycoerythrin (PE) conjugated goat anti-human IgG Fc secondary antibody (Invitrogen, Cat#12-4998-82) and PE conjugated mouse anti-PSGL-1 mAb (BD Biosciences ...
-
bioRxiv - Immunology 2022Quote: ... and 1:5,000 diluted goat anti-human-IgG Fc horseradish peroxidase (HRP) conjugated secondary antibodies (Invitrogen) were added ...
-
bioRxiv - Cancer Biology 2022Quote: ... competition was detected by HRP-goat anti-human IgG Fc secondary antibody (A18817, Thermo Fisher Scientific). The kinetics of the antibody fragments were determined using a Biacore X100 (GE Healthcare) ...
-
bioRxiv - Bioengineering 2023Quote: ... binding was quantified by measuring the fluorescence of a PE-conjugated anti-human Fc antibody (Invitrogen) detecting the Fc-fused protein target ...
-
bioRxiv - Bioengineering 2023Quote: ... HRP-conjugated goat anti-human IgG-Fc (10000x dilution) was used for detection (Invitrogen Cat# 31413). Immobilon Western Chemiluminescent HRP Substrate (Millipore Sigma ...
-
bioRxiv - Microbiology 2019Quote: ... CR-Fc or DC-SIGN-Fc (Thermofisher) in binding buffer (BB ...
-
bioRxiv - Biophysics 2019Quote: Human embryonic kidney cells 293 (HEK293-6E, NRC, Canada) were cultured in FreeStyle F17 expression medium (Thermo Fisher Scientific, Waltham, MA) supplemented with 2 mM L-glutamine (Invitrogen ...
-
bioRxiv - Cell Biology 2021Quote: ... N-terminal FLAG-tagged human MCC (P23508-1) constructs (pCMV2B vector) were transfected into HEK293 cells and selected with G418 (Gibco #10131035). G418-resistant cells were grown ...
-
bioRxiv - Biophysics 2021Quote: Human wild type ABCG2 or ABCG2 R184A containing an N-terminal Flag-tag was expressed in HEK293-EBNA (Thermo Fisher Scientific) cells as previously described19 ...
-
bioRxiv - Molecular Biology 2021Quote: ... the coding sequence encoding human TSPO (hTSPO) was amplified from cDNA previously generated by reverse transcription from total HEK293 mRNA isolated using Trizol (Life Technologies) and cloned into either a pENTR/SD/TOPO vector (Invitrogen ...
-
bioRxiv - Genetics 2024Quote: Flag-tagged wild type and mutated human RBBP5 plasmid were transfected to HEK293 cells by lipofectamine 3000 according to manufacturer’s protocol (Invitrogen, CA, USA). Cells were lysed by RIPA buffer and 20μg of whole cell lysate were used to assess protein expression in Western blot ...
-
bioRxiv - Molecular Biology 2024Quote: ... HEK293 and MS2-tagged HEK293:24xMS2-NEAT1 cells were cultured in Dulbecco’s modified eagle’s medium (DMEM, Gibco) with 10% fetal bovine serum (FBS ...
-
bioRxiv - Pathology 2020Quote: ... cells were first synchronized overnight in 1% FCS DMEM and then cultured for 14 days in 10% FCS DMEM and stimulated daily with IL-1β (1 ng/ml; Invitrogen) or IL-17 at (2.5 ng/ml ...
-
bioRxiv - Microbiology 2019Quote: Human IL-18 and TNFα were measured with the human IL-18 ELISA Kit (MBL, Woburn, Massachusetts) and human TNFα ELISA Kit (ThermoFisher, Waltham, Massachusetts) according to the manufacturers’ instructions using cell culture supernatant at a 1:5 dilution ...
-
bioRxiv - Immunology 2022Quote: ... 1% MEM NEAA and 50 Units/mL recombinant human IL-2 (Myltenyi; #130-097-745) with Dynabeads™ Human T-activator CD3/CD28 (Gibco, #11132D) the same day of the purification (day 0 ...
-
bioRxiv - Microbiology 2021Quote: ... The cells were then stimulated with 20 ng/mL of recombinant human IL-4 (Gibco) and 20 ng/mL of recombinant human IL-13 (Sigma-Aldrich ...
-
bioRxiv - Cancer Biology 2020Quote: ... One ng/mL recombinant human interleukin (IL)-6 (Gibco, Thermo Fisher Scientific, Waltham, MA, USA), was added to the media of the IL-6 dependent myeloma cell lines INA-6 ...
-
bioRxiv - Immunology 2022Quote: ... 2-mercaptoethanol (RP-10) and 10 ng/ml recombinant human IL-2 (Thermo Fisher Scientific) for 18-22 hours ...
-
bioRxiv - Biochemistry 2021Quote: ... supplemented with 10% FCS (FCS, Thermo Fisher Scientific), 100 U ml−1 penicillin ...
-
bioRxiv - Molecular Biology 2021Quote: ... ELMSAN1 TRERF1 DKO HEK293 cells were generated from wild-type Flp-In T-REx HEK293 cells (Invitrogen, R78007) by the Center for Advanced Genome Engineering (CAGE ...
-
bioRxiv - Microbiology 2021Quote: ... IL-22 (IL-22JOP, Thermofisher), IFNγ (XMG1.2 ...
-
bioRxiv - Cell Biology 2020Quote: HEK293 Flip-In TREx cells (Thermo Fisher) were cultured at 37°C with 5% CO2 in DMEM (Thermo-Fisher ...
-
bioRxiv - Biochemistry 2020Quote: HEK293-F cells (FreeStyle™, ThermoFisher #R79007) were maintained in suspension culture according to manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2019Quote: ... HEK293T or HEK293 GnTI- cells (Thermo Fisher) were incubated with 200 nM (monomer concentration ...
-
bioRxiv - Molecular Biology 2021Quote: ... HEK293 Flp-In T-REx 293 (Invitrogen), was grown at 37°C in DMEM high glucose supplemented with 5% Fetal Bovine Serum ...
-
bioRxiv - Cell Biology 2022Quote: HEK293-Flp-In T-Rex cells (Invitrogen) were cultured in high glucose DMEM containing 10% FBS at 5% CO2 ...
-
bioRxiv - Cell Biology 2021Quote: ... HEK293 cells were cultured in DMEM (Gibco) supplemented with 10% FBS and 1% penicillin/streptomycin ...
-
bioRxiv - Cell Biology 2021Quote: HEK293 cells grown in DMEM media (Gibco) supplied with 10%FBS and 2mM L-Glutamine were seeded onto coverslips in 24 well plates and allowed to adhere and grow overnight at 37°C incubator supplied with 5% CO2 ...
-
bioRxiv - Cell Biology 2021Quote: ... HEK293-FT cells were purchased from Invitrogen. U87MG cells were Purchased from the ATCC and cultured as previously described (Sabag et al. ...
-
bioRxiv - Immunology 2020Quote: ... HEK293 cells was cultured in DMEM (Invitrogen) supplemented with 10% FBS at 37 °C and 5% CO2 environment ...
-
bioRxiv - Cancer Biology 2022Quote: ... Flp-In T-REx HEK293 cells (ThermoFisher) were cultured in DMEM (Gibco) ...
-
bioRxiv - Molecular Biology 2023Quote: Flp-In 293 cell line (HEK293; ThermoFisher Scientific ...
-
bioRxiv - Molecular Biology 2023Quote: HEK293 cells were maintained in DMEM (Gibco) supplemented with 10% FBS (Gibco ...
-
bioRxiv - Genetics 2023Quote: HEK293 cells (Thermo Fisher, Waltham, MA, USA) were cultured at 37°C in Dulbecco’s Modified Eagle’s Medium containing high levels of glucose (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2023Quote: HEK293 cells were grown in DMEM (Gibco) containing penicillin (100 U/mL ...
-
bioRxiv - Genetics 2024Quote: ... HEK293 cells were cultured in DMEM (Gibco) supplemented with 10% FBS (Gibco ...
-
bioRxiv - Cancer Biology 2024Quote: ... HEK293 cells were grown in DMEM (Gibco) medium with 10% FBS ...
-
bioRxiv - Microbiology 2024Quote: HEK293 cells were maintained in DMEM (Invitrogen) supplemented with 10% FBS (Cytiva) ...
-
bioRxiv - Molecular Biology 2024Quote: Flp-InTM T-RexTM HEK293 (Thermo Fisher), Flp-InTM T-RexTM HeLa (gift from S ...
-
bioRxiv - Immunology 2024Quote: ... the blots were probed for the presence of heavy and light chain bands using either horseradish peroxidase (HRP)-conjugated polyclonal goat anti-human IgG Fc antibody or polyclonal goat anti-human kappa chain antibody (Thermo Fisher Scientific, Waltham, MA, USA). Antibody-coupled HRP activity was detected with Supersignal West Dura Extended Duration Substrate (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2019Quote: ... the human IL-2 ELISA kit was used in accordance with manufacturer’s instructions (Thermo Fisher Scientific). Murine IL-2 ELISA’s were performed using the mouse IL-2 ELISA kit (Thermo Fisher Scientific).
-
bioRxiv - Immunology 2023Quote: ... The supernatant was collected on day 3 and analyzed by human IL-2 ELISA Kit (ThermoFisher) according to the manufacturer’s protocol.
-
bioRxiv - Immunology 2021Quote: Human ACE2-transfected HEK293 cells (prepared in-house) were collected and washed with FACS buffer supplemented with 2% FBS (GIBCO, CN: 10099-148) in 1X PBS ...
-
bioRxiv - Cancer Biology 2021Quote: Cells (1-1.5×10^6) were incubated with FACS sample buffer (DPBS, 0.5% FBS) and human FC Block (Invitrogen) for 20 minutes on ice followed by incubation at 4°C for 30 minutes with the following - antibodies ...