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Citations for Thermo Fisher :
701 - 750 of 3080 citations for IFT122 siRNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... cells were transfected with 50 nM siRNA SMARTpools or individual siRNA using lipofectamine RNAi-Max transfection reagent (13778150, Invitrogen, Paisley, UK) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: For siRNA transfections conducted in a 24-well plate 5pmol siRNA was incubated in Opti- MEM Reduced Serum Media (Gibco, 31985062) with Lipofectamine RNAiMax Transfection Reagent (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... Each nAChR siRNA or control siRNA was co-transfected with BLOCK-iT™ Alexa Fluor Red Fluorescent Control (Thermo Fisher Scientific) to the cells using Lipofectamine RNAiMAX Reagent (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... or a non-targeting control siRNA (siGENOME Non-Targeting siRNA 5, Horizon Discovery, # D-001210-05-05) and 5 µl Lipofectamine RNAiMAX (Invitrogen, #13778075) in a total volume of 2 ml in antibiotics free 10% DMEM ...
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bioRxiv - Molecular Biology 2020Quote: Down-regulation of Tspan1 in BxPC3 cells and PNAC-1 cells was achieved using specific siRNAs targeting Tspan1 (Tspan1 siRNA, cat.no.4392420; Thermo Fisher Scientific, Inc.). The scramble-siRNA (siNC ...
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bioRxiv - Cancer Biology 2021Quote: ... 5’ CAGACAGUGCAGUAUUCACTT 3’) and control siRNA (#4427037) as in (56) and OSR1 siRNA (s19303 Silencer® Select) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were grown to ~70% confluency in complete DMEM for 1 day in a 6-well plate and transfected with siRNA for each well as follows: 30 pmol of siRNA and 1 μl of Lipofectamine™ RNAiMAX Transfection Reagent (#13778030, Thermo Fisher) were diluted separately in two vials of 150 μl Opti-MEM (#31985062 ...
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bioRxiv - Cell Biology 2021Quote: ... or siGENOME SMARTpool siRNA D-019800-17 DSP (Dharmacon, Lafayette, CO) (siDP1) or stealth siRNA oligonucleotides 5′-CAGGGCUCUGUCUUCUGCCUCUGAA-3′ from Life Technologies (siDP2) using the Amaxa Nucleofector System (Lonza ...
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bioRxiv - Molecular Biology 2021Quote: ... Transfections were carried out in AML12 cells in a 12-well or 6-well plate or four-well chambered slides or 24-well Seahorse XF plate using 30 nM of the above indicated siRNAs and negative control siRNA with Lipofectamine RNAiMAX (Invitrogen; Life Technologies Inc.) following the reverse transfection protocol ...
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bioRxiv - Genomics 2020Quote: ... and LECs were treated for additional 24h with 20nM of high GC scramble control siRNA or two siRNAs targeting KLF4 (Thermo Fisher Scientific) and 32µL Lipofectamine RNAiMAX previously mixed in 800µL Opti-MEM.
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bioRxiv - Biochemistry 2019Quote: ... Cells were transfected with 120 ng PEX3-mEMerald-FKBP and BicD2NS-V5-FRB constructs per well as well as 20 pmol of either ON-TARGETplus Non-targeting siRNA #1 (Dharmacon) or SMARTpool: ON-TARGETplus PAFAH1B1 siRNA (Dharmacon) using Lipofectamine 2000 (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2019Quote: ... The cells were transfected after 24h with 30pmol concentration of siRNA (Integrated DNA Technologies) or scrambled control (Mission siRNA Universal Negative Control) using Lipofectamine® RNAiMAX Reagent (Invitrogen/Life Technologies) in Gibco™ OptiMEM ...
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bioRxiv - Physiology 2020Quote: ... A transient knockdown experiment was performed by transfecting siRNAs against Angptl4 and Ampk (sets of 4 SMART POOL siRNAs, Dharmacon, USA) in HepG2 cells using RNAiMAX (Life Technologies, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Differentiating monocytes at day 2 of culture were transfected with two different MyD88 Silencer Select Validated siRNA or with a control siRNA (all at 50 nM final concentration; Ambion, Thermo Fisher Scientific) using Opti-MEM I reduced serum medium and Lipofectamine RNAiMAX transfection reagent (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... BMDMs were transfected after 6 days of differentiation with 25nM ON-TARGETplus SMARTpool Fads2 siRNA (Dharmacon) or the same concentration of non-targeting siRNA pool (siCtrl) using lipofectamine RNAiMax (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The 293T cells were transfected with a pool of 3 Nrf2-targeting siRNAs (TGACAGAAATTGACAACAA; AGACAAGAACAACTCCAAA; ACAGTGTCTTAACATTCAA) or control siRNA (CAAACAGAAUGGUCCUAAA) (50mM) using Lipofectamine™ RNAiMAX transfection reagent (Invitrogen, 13778100) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... Cells were then incubated with transfection mixtures containing 75 nM of siRNAs or Silencer Select Negative Control-1 siRNA (Ambion, Austin, TX) for 4 h ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then treated with either 10nM of non-silencing control siRNA or 10 nM PCSK9 siRNA using Lipofectamine RNAiMAX reagent (ThermoFisher, cat# 13778030). Cells were incubated at 37C ...
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bioRxiv - Cell Biology 2020Quote: SMC were transfected with siRNA to ATM, ATR, DNA-PK or scrambled control (20 nM, ONTARGETplus siRNA, Dharmacon) using Lipofectamine 2000 reagent (Thermo Fisher Scientific), according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... cells were transfected with a 100 nM suspension of relevant siRNA duplexes (Eurofins) or siRNA SMARTpools (Dharmacon) using Lipofectamine RNAi-MAX transfection reagent (13778150, Invitrogen, Paisley, UK) as per manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2020Quote: Cells were transfected by adding 1 ml serum-free culture media with Trans-IT X2 transfection reagent (MirusBio) and targeting (On-Target Smartpool siRNA) or scrambled (siCTRL) siRNA (Dharmacon, Thermo Fisher Scientific) twice on two consecutive days ...
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bioRxiv - Physiology 2022Quote: Optimal siRNA concentration (30 nM) and conditions for small interfering RNA (siRNA) transfection using Lipofectamine RNAiMAX lipid reagent (Invitrogen, Carlsbad, CA, USA) were previously established (Santin et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were nucleofected with 100 nM of the above described siRNA NTC and siRNA SOX11 using the Neon Transfection System (Thermo Fisher Scientific) and subsequently seeded in 6-well or T25 tissue culture plates.
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bioRxiv - Genetics 2022Quote: ... cells were transfected with 50nm siRNA CTRL or siRNA GLB1 (IDT, sequence available in Table S1) with lipofectamine RNAimax (Thermo fisher scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... The screen used reverse transfection of siRNAs in which all wells had 250 nM siRNA concentration in 10 µL of Opti-MEM (Gibco; Thermo Fisher Scientific). Lipofectamine2000 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: To transfect SW480 cells, 50 nM LinCode LincRNA siRNA or negative control siRNA (Dharmacon, Lafayette, CO) was diluted in warm Opti-MEM (Gibco, Waltham, MA) and 11 µL/mL of Lipofectamine RNAiMax (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: Pre-designed and validated specific small interfering RNA (siRNA) oligos for knockdown of endogenous SRRM1 (#s20018; Silencer® Select siRNAs; ThermoFisher Scientific) were used ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... A second siRNA oligo (McIdas siRNA_2) was also used for McIdas depletion: 5’-CAACTGCACGTGACATTGATT-3’ (s51151, Silencer® Select siRNA, Thermo Fisher Scientific). The Silencer® Select No ...
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bioRxiv - Cell Biology 2019Quote: ... cells were grown to 90% confluence and transfected with 20 nM Piezo1-specific Silencer Select Pre-designed siRNA (#4392420, siRNA ID: s18891, Thermo Fisher Scientific) or ON-TARGETplus Non-targeting Pool siRNA (#D-01810-10-20 ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were transfected with Silencer Select Pre-Designed siRNAs either as negative control (non-targeting, Silencer Select Negative Control No. 1 and 2 siRNA) or NFIB-targeting siRNA constructs (Thermofisher, s9494, s9496) using 30 pmol of siRNA in 9 μl of Lipofectamine RNAi Max ...
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bioRxiv - Molecular Biology 2021Quote: ... HeLa cells in 35-mm dishes were transfected with 100 pmol of each siRNA in the Stealth siRNA library targeting 154 human nuclear proteins (Invitrogen–Thermo Fisher Scientific) using Lipofectamine RNAiMax (Invitrogen–Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: 8,000 cells were transfected with 4.8 pmoles of ASH2L siRNA (Dharmacon, M-019831-01-0005) or non-targeting siRNA (Dharmacon, D-001210-02-05) using Lipofectamine RNAiMax (Invitrogen, 13778-150) in OPTI-MEM I Reduced serum medium (Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... siRNAs targeting three regions within the Stmn2 mRNA (Cat# s73356, s73354, s73355) were chosen from pre-designed mouse siRNAs (Silencer siRNA, Thermo Fisher Scientific). The control group was treated with Mission siRNA Universal Negative Control # 1 (Cat# SIC001 ...
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bioRxiv - Cancer Biology 2021Quote: ... siRNA and the negative control siRNA (siNC) with 20 nM in 6-well plates were transfected using Liopfectamine 3000 (Invitrogen, Carlsbad, USA) according to the manufacturers’ protocols ...
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bioRxiv - Cell Biology 2021Quote: ... or 5 nM Silencer select siRNAs (Silencer Select Negative Control #1 siRNA, 4390843; OMA1, s41776; LONP, s17903; PTPMT1, s229947) (Thermo Fisher Scientific) were transfected using Lipofectamine RNAi max transfection reagent (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2020Quote: 3×104 ESF cells were plated per well of a 24-well plate and 18 hours later cells were transfected in Opti-MEM with 10nM (per well) of siRNA targeting HAND2 (s18133; Silencer Select Pre-Designed siRNA, cat#4392420; Thermo Fisher Scientific) or IL15 (s7377 ...
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bioRxiv - Immunology 2021Quote: Two different siRNA for thymidylate synthase (TYMS silencer select s14538, s14539) and one control siRNA (silencer select Negative Control #1) (Ambion, Life Technologies) were used at 100 nM ...
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bioRxiv - Genomics 2019Quote: ... MPO and GRN as well as 500nM of siRNA targeted against GP5 or control eGFP siRNA oligonucleotides using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... cells at 60–70% confluence were transfected with 10 nM of siRNA targeting circGLIS3 or a scrambled siRNA for 24 and 48 hours using Lipofectamine™ 3000 (ThermoFisher Scientific). To overexpress circGLIS3 ...
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bioRxiv - Molecular Biology 2022Quote: ... we transfected the third passage of keratinocytes at 60-70% confluence with a 10 nM siRNA pool targeting HOXC13-AS or negative control siRNAs (Supplementary Table 3) for 24 hours with Lipofectamine™ 3000 (ThermoFisher Scientific). To identify the function of HOXC13-AS-bound proteins ...
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bioRxiv - Cell Biology 2022Quote: HUVECs expressing PIK3CA p.H1047R or PIK3CA wt were transfected with a Silencer Select siRNA targeting to TGFA (ID: s14053) or negative control siRNAs (#1 and #2 mixed in ratio 1:1; all siRNA oligonucleotides from Thermo Fisher Scientific) as previously described (58) ...
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bioRxiv - Microbiology 2023Quote: ... and two Silencer Select negative-control siRNAs (Silencer Select negative control no. 1 and no. 2 siRNA) were purchased from Ambion (Life Technologies). Three days before infection ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA from transfected U251 and U343 cells (siRNA control vs. siRNA MAGOH/MAGOHB) was extracted using the TRIzol reagent (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... human FLOT2 (ON-TARGETplus Human FLOT2 siRNA SMART POOL, L-003666-01-0010) (Horizon Discovery Ltd.), non-targeting control siRNA (#4390843, 40 nM) (Thermo Fisher Scientific). In brief ...
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bioRxiv - Cell Biology 2023Quote: ... 5’-GAGACCCUAUCCGUGAUUAtt-3’ and antisense: 5’- UAAUCACGGAUAGGGUCUCtt-3’ (Silencer Select, rat negative control #1; scrambled siRNA and all siRNAs were from Ambion, Life Technologies). Transfection complexes were prepared in accordance with the instructions provided by the manufacturer and added to 2×105 cells seeded per well in 24-well plates.
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SHARPIN enhances ferroptosis in synovial sarcoma cells via NF-κB- and PRMT5-mediated PGC1α reductionbioRxiv - Cancer Biology 2023Quote: ... cells were transfected with an siRNA targeting SHARPIN or with a corresponding scrambled siRNA (final siRNA concentration: 10 nM) (SMARTpool reagents, Horizon Discovery) using Lipofectamine RNAiMAX Transfection Reagent (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2023Quote: ... The cells were transfected with MRPP1 siRNA1 and siRNA3 along with a scrambled siRNA as a negative control (final siRNA concentration 30 nM) using Lipofectamine RNAiMAX reagent (Invitrogen, 13778-075). Galactose media were prepared by using DMEM without glucose (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: ... A nontargeting siRNA scramble control (siNC) and siRNA targeting CCR5 (siCCR5) were transfected into TNBC cells using lipofectamineTM 2000 transfection reagent (ThermoFisher Scientific, 11668019) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: The role of Nrf2 signaling in MEF cells was investigated by inhibiting Keap1 using small interfering RNA (siRNA; siRNA mouse silencer select, Keap1 ID: s78526; Thermo Fisher Scientific). MEFs at 70% confluence were plated in 6-well plates 24 hours before transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... HeLa cells (in 100-mm dishes) were transfected with 500 pmol SAP30BP siRNA or negative control siRNA (Nippon gene) using Lipofectamine RNAiMAX (Invitrogen–Thermo Fisher Scientific) according to the manufacturer’s protocol ...