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Citations for Thermo Fisher :
201 - 250 of 10000+ citations for Human Procollagen II C Terminal Propeptide PIICP CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... using the BP Clonase II kit (ThermoFisher Scientific). Plasmid DNA from a sequence-verified clone was then recombined into pET-DEST42 ...
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bioRxiv - Cell Biology 2023Quote: ... the Cells-to-cDNA II Kit (ThermoFisher Scientific) was used for RNA isolation with cDNA synthesis as described (62) ...
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bioRxiv - Molecular Biology 2023Quote: ... using the Gateway LR Clonase II kit (Invitrogen). Integrity and orientation of inserts was checked further by Sanger sequencing before using plasmids to generate transgenic Drosophila.
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bioRxiv - Cell Biology 2020Quote: ... The cells were labeled with the rabbit anti-tau (C-terminal) antibody (MilliporeSigma) or the mouse anti-phospho-Tau (Thr181) Clone AT270 antibody (Thermo Fisher Scientific) at room temperature at dilution of 1:1000 in 1% BSA for 1 hour and then labeled with Alexa Fluor488 goat anti-mouse IgG secondary antibody at a dilution of 1:500 for 30 minutes at room temperature ...
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bioRxiv - Genetics 2021Quote: ... aliquots of a custom-made rabbit anti-NHE6 antibody (C-terminal epitope: GDHELVIRGTRLVLPMDDSE, Covance #048) (Ouyang et al., 2013) were conjugated to Dynabeads Protein G (Thermo Fisher #10004D) at room temperature for 2 h according to the manufacturer’s instructions ...
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Interaction of the Xanthomonas effectors XopQ and XopX results in induction of rice immune responsesbioRxiv - Molecular Biology 2020Quote: ... from the pENTR clones to the BiFC vector pDEST-VYCE(R)GW carrying the C-terminal region of the Venus Fluorescent Protein (VFP) (Gehl et al., 2009) by Gateway® cloning (Invitrogen, California) from the pENTR clones to yield the constructs as listed in Supplementary table S2 ...
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bioRxiv - Biophysics 2019Quote: ... the MerMAID1 gene was subcloned with a C-terminal TEV protease restriction site and a 6× His-Tag into the pPiCZ vector (Invitrogen, Carlsbad, CA). Zeocin™-resistant positive clones were selected from electroporation-transformed yeast cells ...
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bioRxiv - Biochemistry 2020Quote: Recombinant constructs for NT-mini (Gly497-Leu875) and C-terminal Agrin substrates (Pro1635-Pro2067) were amplified using a Phusion DNA polymerase (Thermo Fisher Scientific) starting from a source synthetic gene (GeneWiz ...
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bioRxiv - Plant Biology 2020Quote: ... each slide was incubated overnight at 4°C with 100 μL of rabbit polyclonal anti-β-glucuronidase antibody (N-Terminal, 5420, Molecular Probes, Invitrogen) diluted 1:200 in fresh blocking buffer (3% BSA in 1X PBS) ...
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bioRxiv - Cell Biology 2020Quote: ... For transient overexpression (Fig 2 E-H, S2G) seipin tagged with C-terminal EGFP [34] was inserted into pcDNA5/FRT/TO (Thermo Fisher Scientific) through HindIII/NotI sites ...
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bioRxiv - Cancer Biology 2021Quote: ... Dominic Esposito Lab) along with mPol2 promoter and C-terminal V5 tag entry vectors using the gateway LR clonase reaction (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2021Quote: The SFI1 antibody was raised in rabbit against the GST-fused C-terminal domain of SFI1 (aa1021 to aa1240) (Figure S1) and affinity-purified on AminoLink® Coupling Resin (20381 Thermo Fisher) coupled to the MBP-fused C-terminal domain (using the same aa sequence).
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bioRxiv - Neuroscience 2020Quote: The C-terminal region of Spg11 (amino acid residues 1468-1815) was amplified using Phusion Flash High-Fidelity polymerase (Thermo Fisher F548L) and extracted from electrophoresis gel using QIAquick Gel Extraction Kit (Qiagen 28704 ...
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bioRxiv - Microbiology 2022Quote: ... Cells were transfected with 2 μg of phCMV3 plasmids encoding the C-terminal HA-tagged NA from the strain of interest using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were transferred from the gel onto a polyvinylidene difluoride membrane [36] and detected with the anti-His (C-terminal)-HRP antibody (Thermo Fisher/Invitrogen) according to the manufacturer's instructions ...
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bioRxiv - Immunology 2021Quote: All SARS-CoV-2 S NTD domain constructs (residues 14-307) with a C-terminal 8XHis-tag were produced in 100 mL culture of Expi293F™ Cells (ThermoFisher Scientific) grown in suspension using Expi293™ Expression Medium (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: The expression plasmid encoding for codon-optimized SARS-CoV-2 RBD (329-538) with a C-terminal hexa-histidine tag was transfected into Expi293™ GnTI-(Thermo Fisher) cells (1×106 cells/mL ...
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bioRxiv - Bioengineering 2021Quote: ... sequence coding for the amino acid 319-541 and fused with the N-terminal SARS-CoV-2 spike secretory signal and a C-terminal hexa-histidine tag was transfected into 293F cells cultivated in Freestyle 293 expression media (Thermo Fisher, #12338018) at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... the expression plasmid encoding for codon-optimized SARS-CoV-2 RBD (329-538) with a C-terminal hexa-histidine tag was transfected into Expi293™ GnTI- (Thermo Fisher) cells (1×106 cells/mL ...
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bioRxiv - Microbiology 2023Quote: ... was cloned into pFastBac with a C-terminal His6-tag for expression in Sf9 insect cells using the Bac-to-Bac™ Baculovirus Expression System (Thermo Fisher). Following transformation of DH10Bac cells ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... ORP9 and ORP11 cDNAs were custom synthesized by Twist Biosciences to include a C-terminal V5 tag and attB sites and cloned into pDONR221 (Thermo Fisher, 12536017). ORP1 cDNA was purchased in a Gateway compatible pGenDONR vector from GenScript ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... mammalian expression vector with an amino-terminal protein C tag followed with a 3C protease cleavage site and transfected into Expi293 cells (Thermo Fisher Scientific) using FectoPRO (Polyplus-transfection ...
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bioRxiv - Biochemistry 2024Quote: ... and cloned into a pRK5-derived plasmid with a C- or N-terminal FLAG tag using the gateway vector conversion system (Invitrogen, cat # 11828029). All gene constructs were verified by DNA sequencing.
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bioRxiv - Neuroscience 2020Quote: Levels of total APOE protein were analysed by Human ELISA Kit from Invitrogen (Apolipoprotein E Human ELISA Kit). Levels of Aβ were analysed by ELISA (Human β Amyloid 42 ELISA Kit Wako high sensitive #298-64401 and Human β Amyloid 40 ELISA Kit Wako #298-64601 ...
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bioRxiv - Plant Biology 2022Quote: ... approximately 700 ng of total RNA was denatured at 65°C for 5 min and then reverse-transcribed using the Super-Script II kit (Invitrogen, Carlsbad, CA, USA) at 25°C for 10 min ...
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bioRxiv - Neuroscience 2022Quote: ... the human Aβ42 Ultrasensitive ELISA Kit (Invitrogen) was used according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... the Human Osteopontin ELISA kit (Thermo Fisher) was used following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... and the HGF Human ELISA Kit (Invitrogen) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Human TNF-α Ultrasensitive ELISA Kit (Invitrogen,KHC3014 [96 tests] and Human IFNα ELISA Kit [Invitrogen,BMS216TEN] ...
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Kinetics Of Interferon-λ And Receptor Expression In Response To In Vitro Respiratory Viral InfectionbioRxiv - Immunology 2021Quote: ... A549 cells (human type II alveolar epithelial line) were cultured in F12K medium (Gibco, USA) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Biochemistry 2021Quote: ... 5% (v/v) human serum (PAA Laboratories GmbH) and 5 % v/v Albumax II (Invitrogen) at 37°C in a gas environment of 5 % CO2 ...
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bioRxiv - Genomics 2021Quote: ... and HLA-DR/human MHC class II antibodies (Invitrogen Dynabeads®, Life Technologies AS, Norway). An initial positive selection was done with CD3 beads followed by a positive HLA-DR selection ...
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bioRxiv - Bioengineering 2020Quote: ... and c-Kit(Invitrogen, NOVUS Biological) were conducted ...
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bioRxiv - Cell Biology 2023Quote: ... and c-kit–APCeFluor780 (Thermo Fisher), Sca1-PB (Biolegend) ...
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bioRxiv - Biochemistry 2023Quote: The ibpA 5’ UTR-gfp mRNA produced from CUGA®7 in vitro transcription kit (Nippon Gene) was attached to a 3’-terminal biotinylated nucleotide using Pierce™ RNA 3’ End Biotinylation kit (Thermo Scientific). The biotin-labeled mRNA (0.1 µM ...
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bioRxiv - Biophysics 2019Quote: ... The construct was cloned into a pFastBac vector with a C-terminal FLAG affinity tag and used for baculovirus production according to manufacturers’ protocol (Invitrogen, Bac-to-Bac). The protein was expressed by infecting Sf9 cells with baculovirus at a density of 1.3M cells ml-1 and incubating at 27° C for 72 hours in an orbital shaker ...
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bioRxiv - Plant Biology 2020Quote: ... each slide was incubated overnight at 4°C with 100 μL of rabbit polyclonal anti-β-glucuronidase antibody (N-Terminal, 5420, Molecular Probes, Invitrogen) diluted 1:200 in fresh blocking buffer (3% BSA in 1X PBS) ...
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bioRxiv - Cell Biology 2020Quote: ... was expressed with a C-terminal Myc-DDK tag from a pCMV6-Entry backbone in Expi 293F suspension cells (Thermo Fisher Scientific, A14527). Cells were grown at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... HCV nsp5b RdRp was purified using Ni-NTA affinity chromatography based on the C-terminal eight-histidine tag according to the manufacturer’s specifications (Thermo Scientific, Rockford, IL, USA). NiV RdRp P/L complex coding sequence (AEZ01385 and AEZ01390 ...
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bioRxiv - Microbiology 2020Quote: ... and a C-terminal His8 tag followed by an AviTag (39) cloned into the pcDNA-3.4 vector backbone (Thermo Fisher, Waltham, MA, USA). Constructs encoding tagged human PDGF-B (residues 82–190 ...
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bioRxiv - Biophysics 2024Quote: Labeling of C-terminal Cysteine on RepA-Titinx was carried out using a C2-maleimide reaction with Alexa Fluor™ 555 fluorophore (Invitrogen, Waltham, MA) following the manufacturer’s protocol except 3 (fluorophore ...
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bioRxiv - Immunology 2022Quote: ... with terminal deoxynucleotidyl transferase (Thermo Fisher Scientific: EP0161) as described in Gaspar et al. ...
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bioRxiv - Biochemistry 2020Quote: ... Terminal deoxy transferase (TDT) was obtained from ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... and terminal deoxynucleotidyl transferase (TdTase ThermoFisher Scientific #10533065). The reaction was kept at room temperature for 3-4 days and poly-dT was purified using phenol chloroform extraction and suspending the air-dried pellet in water ...
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bioRxiv - Immunology 2021Quote: ... with terminal deoxynucleotidyl transferase (Thermo Fisher Scientific: EP0161) as described in (Gaspar et al. ...
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bioRxiv - Immunology 2021Quote: ... with terminal deoxynucleotidyl transferase (Thermo Fisher Scientific: EP0161) as described in (34).
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bioRxiv - Molecular Biology 2023Quote: ... they reacted with terminal deoxynucleotidyl transferase enzyme (Invitrogen) and biotinylated 16-dUTP (Roche Diagnostics ...
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bioRxiv - Cell Biology 2024Quote: ... and terminal deoxynucleotidyl transferase (Thermo Fisher Scientific: EP0161) as described in Gaspar et al ...
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bioRxiv - Plant Biology 2020Quote: ... the fragments were cloned into entry vector pDONR207 and yeast expression vector pYesdest52 using Gateway BP Clonase II Enzyme Kit and LR Clonase II Enzyme Kit (Invitrogen, MA, USA), respectively.
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bioRxiv - Genetics 2020Quote: The pCMV-c-JUN-MYC plasmid was generated by cloning the human c-JUN coding sequence in the pCMV-MYC plasmid (ThermoFisher). Primers used for generation of the construct are shown in supplementary table S5 ...