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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Human Procollagen II C Terminal Propeptide PIICP CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... were cloned into pCEP4 mammalian expression vector containing N-terminal human Ig kappa leader sequence and C-terminal Avi-tag and His-tag (Invitrogen, USA). Expi293-Freestyle cells cultured at 37°C and 8% CO2 in growth medium containing Expi293 Expression Medium at 3×106/mL in 50 mL media were transfected overnight at 37 °C with 50μg of plasmid in 160μL of ExpiFectamine plus 6mL of OptiMEM-I (all from Invitrogen) ...
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bioRxiv - Cell Biology 2019Quote: ... and pENTR-Megator constructs with pHWG (blasticidinR; C-terminal EGFP tag) using Gateway LR Clonase II (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... with either a C-terminal (Thermo Scientific) or N-terminal poly-histidine-tag (Genscript ...
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bioRxiv - Molecular Biology 2020Quote: ... vector using Gateway cloning in frame with the C-terminal V5 tag (Gateway LR Clonase II mix; Life Technologies). The Gli2 P1-6A mutant was previously described (15) ...
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bioRxiv - Plant Biology 2023Quote: ... into the plant expression vectors pB7YWG2 (35S promoter; C-terminal YFP) or pB7RWG2 (35S promoter; C-terminal RFP) using Gateway LR clonase (Invitrogen). To generate the chloroplast-targeted YFP and RFP proteins ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-APP C-Terminal (clone CT695; Invitrogen), mouse anti-APP N-terminal (clone 22C11 ...
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bioRxiv - Cancer Biology 2024Quote: ... FOXA1 C-terminal (Thermo Fisher Scientific: PA5-27157); AR (Millipore ...
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bioRxiv - Plant Biology 2019Quote: ... or the stop codon-less full CDS of PNPAR was recombined into the destination vector pGWB651 for C-terminal fusion with GFP or pGWB617 for C-terminal fusion with 4xMYC through Gateway LR reaction (Thermo Scientific) to create p35S:tPNP-A-GFP and p35S:PNPAR-4xMYC ...
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bioRxiv - Microbiology 2022Quote: ... The two pAcgp67-RBD (residues 333–530) plasmid with a C-terminal 8×His tag were transfected into Sf9 cells using Cellfectin II Reagent (Invitrogen) to produce the recombinant baculoviruses ...
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bioRxiv - Plant Biology 2023Quote: ... with a constitutive CaMV 35S promoter and either a YFP or CFP C-terminal tag using the LR Clonase II enzyme mix (Invitrogen). The YFP-tagged construct was used for the generation of stable transgenic Arabidopsis lines of and co-IP experiments ...
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bioRxiv - Developmental Biology 2024Quote: ... Flow cytometry was used to evaluate the efficiency of differentiation to definitive endoderm at day 3 using the endoderm markers CXCR4 and c-KIT (Anti-human CXCR4 PE conjugate, Thermo Fisher, MHCXCR404,1:20; Anti-human c-kit APC conjugate, Thermo Fisher, CD11705 ...
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bioRxiv - Biophysics 2021Quote: A stable β2AR-overexpressing cell line was produced by transfecting CHO-K1 cells (ECACC) with a plasmid coding for the human β2AR with C-terminal GFP using lipofectamine 2000 (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: Mammalian expression plasmids encoding SARS VHH72 and MERS VHH-55 (73) with a C-terminal a monomeric human Fc tag were transfected into ExpiCHO cells (Thermofisher) using ExpiFectamine as manufacterer’s recommendation ...
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bioRxiv - Neuroscience 2020Quote: ... β-neurexin-1 extracellular domain was cloned into the pCAGGS vector with a C-terminal human Fc tag and the ATD regions of GluA1–4 were cloned into a modified pDisplay vector (Invitrogen), as described previously (9) ...
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bioRxiv - Physiology 2023Quote: ... followed by C-terminal region of human FGF-21 (amino acids 166-209) into a vector pCEP4 (Thermo Fisher Scientific). The construct was then transiently transfected to Expi293F cells (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Extracellular fragment of human CD4 comprising domains 1-4 of human CD4 and a C-terminal His6-tag was expressed in Expi293 cells according to the manufactureŕs protocol (Thermo Fisher Scientific). Cell supernatant was harvested by centrifugation 4 days after transfection ...
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bioRxiv - Developmental Biology 2021Quote: ... the cDNA fragment was subcloned from pDONR221 to pAWH (a GATEWAY Destination vector for C-terminal 3xHA tagging, DGRC#1096) using LR Clonase II Enzyme mix (11791020, Thermo Fisher Scientific), resulting in pAWH-nord ...
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bioRxiv - Plant Biology 2020Quote: ... for C-terminal fusion using LR reactions (Thermo Fisher Scientific). Rice protoplasts were transformed with these vectors as described previously (Wong et al ...
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bioRxiv - Immunology 2021Quote: ... or for intracellular expression using the pYES2/NTC vector (N-terminal Xpress and C-terminal V5 epitope, Invitrogen). The pYD1 system used the yeast internal Apa1/Ap2 system ...
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bioRxiv - Microbiology 2022Quote: ... was synthesized as a custom peptide with N-terminal acetylation and C-terminal amidation (Life Technologies Europe Bv).
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bioRxiv - Plant Biology 2022Quote: ... the construct was recombined with pB7FWG2 destination vector (YFP in C-terminal) using LR ClonaseMixII kit (Invitrogen) and introduced into Arabidopsis (sbt3.3 ...
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bioRxiv - Microbiology 2023Quote: ... a mammalian secretion sequence and a C-terminal C- tag) was transiently expressed using Expi293F™ cells using the Expi293™ Expression System Kit (Thermo Fisher) as recommended ...
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bioRxiv - Molecular Biology 2020Quote: ... and hybridized to the Affymetrix Human Gene 1.0 ST Arrays using the Gene Chip WT Terminal Labeling and Hybridization Kit (Affymetrix). GeneChip fluidics station 450 was used for processing of the arrays and fluorescent signals were detected with the GeneChip scanner 3000-7 G ...
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 Beta RBD for crystallization (residues 328-531 of S protein from GenBank NC_045512.2 with N-terminal signal peptide and C-terminal 8xHis-tag) was expressed in Expi293F (Thermo Fisher Scientific) cells in the presence of 10 µM kifunensine at 37°C and 8% CO2 ...
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bioRxiv - Plant Biology 2021Quote: ... or GLK2 was recombined into the destination vector pGWB605 for C-terminal fusion with sGFP or into pGWB617 for C-terminal fusion with 4×Myc through the Gateway LR reaction (Thermo Scientific). For the 35S:GLK1-4×Myc (or sGFP ...
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bioRxiv - Immunology 2021Quote: ... GenBank: QHD43416.1) with an N-terminal IgKappa signal sequence (METDTLLLWVLLLWVPGSTG) and C-terminal TwinStrep- and 3xFLAG-tags (GeneArt, ThermoFisher, Regensburg, Germany) into a pcDNA3.4(+ ...
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bioRxiv - Plant Biology 2022Quote: The full-length coding sequence of the genes were cloned into pSPYNE-35SGW (N-terminal YFP) and/or pSPYCE-35SGW (C-terminal YFP) through gateway cloning (Invitrogen™). BiFC was carried out as described (Gou et al. ...
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bioRxiv - Developmental Biology 2024Quote: HEK293T cells were transferred with C-terminal HA-tagged Kayak and N-terminal GFP-tagged Jun-epi or Kayak using Lipofectamine 2000 (11668030, Thermo Fisher). The GFP-trap agarose beads (ABIN509397 ...
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bioRxiv - Microbiology 2021Quote: ... and one derived from the C-terminal region (SDGGRDEVEKNNGLDVIR, Thermo Fisher, USA) (1:4000 dilution of affinity purified antibody stock of 2.95 mg/ml) ...
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bioRxiv - Microbiology 2021Quote: SARS-CoV-2 RBD WT for SPR binding assays (with N-terminal signal peptide and C-terminal thrombin cleavage site-TwinStrep-8xHis-tag) was expressed in Expi293F (Thermo Fisher Scientific) cells at 37°C and 8% CO2 ...
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bioRxiv - Immunology 2021Quote: ... NC_045512.2 with N-terminal signal peptide and C-terminal thrombin cleavage site-TwinStrep-8xHis-tag) were expressed in Expi293F (Thermo Fisher Scientific) cells at 37°C and 8% CO2 ...
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bioRxiv - Biochemistry 2021Quote: SARS-CoV-2 RBD proteins for SPR binding assays (residues 328-531 of S protein from GenBank NC_045512.2 with N-terminal signal peptide and C-terminal thrombin cleavage site-TwinStrep-8xHis-tag) were expressed in Expi293F (Thermo Fisher Scientific) cells at 37°C and 8% CO2 ...
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bioRxiv - Biochemistry 2021Quote: ... and a C-terminal hexa-histidine tag (GeneArt Gene Synthesis, Thermo Fisher Scientific). The HexaPro expression plasmid was a gift from Jason McLellan (Addgene plasmid #154754 ...
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bioRxiv - Developmental Biology 2022Quote: ... C-terminal HCFC1 (Bethyl labs Cat # 50- 156-0286 purchased through Fisher Scientific) (anti-rabbit ...
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bioRxiv - Cancer Biology 2020Quote: ... and labeled with GeneChip WT Terminal Labeling Kit (Affymetrix) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... Terminal sequences were obtained using 5’ RACE Kit (ThermoFisher) and an anchored oligo-dT primer for the 3’end ...
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bioRxiv - Microbiology 2021Quote: ... [C]-Ahx-QAHAEERLEDIDYVE-acid (Cambridge Research Biochemicals, Billingham, UK) and C-terminal region of FtsZ2: NH2-[C]-SDGGRDEVEKNNGLDVIR-COOH (Thermo Fisher Scientific). H ...
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bioRxiv - Cell Biology 2023Quote: ... and GeneChip WT Terminal labeling Kit and Controls Kit (Thermo Fisher Scientific) following the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2024Quote: ... fixed fibroblasts were incubated with primary antibodies specific to procollagen I (Invitrogen, Waltham, Massachusetts, 1:200), αSMA (sigma ...
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bioRxiv - Microbiology 2021Quote: ... 5% human serum and 5% AlbuMAX-II (Gibco)) was replaced daily until reaching maturity at day 14-post induction ...
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bioRxiv - Cancer Biology 2020Quote: ... RELA with C-terminal HA tag into Gateway donor vector (pCR8/GW/TOPO, Invitrogen). Then LR clonase II reactions were used to shuttle ORFs into pCW-DEST (lentiviral Dox-inducible expression ...
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bioRxiv - Microbiology 2022Quote: ... and a C-terminal isotope-labeled (“heavy” peptide) version was synthesized (Thermo Fisher Scientific).
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bioRxiv - Biophysics 2023Quote: ... The C-terminal cysteine residue was labeled with Alexa 488 C5-maleimide (ThermoFisher Scientific). The labeling was performed in 50 mM phosphate buffer at pH 7 with 100 mM NaCl and 8 M Urea ...
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bioRxiv - Biochemistry 2021Quote: ... mature factor X (FX) protein preceded by the prothrombin signal sequence and propeptide were purchased from GenArt (Thermo Fisher Scientific). N-glycosylation acceptor sites were introduced successfully at modelled surface exposed sites of publicly available crystal structure of FXa (PDB id 1FAX and residues 60 ...
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bioRxiv - Microbiology 2019Quote: ... each fused to a C-terminal HA-tag using lipofectamine 3000 transfection reagent (ThermoFisher, L3000015) according to manufacturer instructions ...
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bioRxiv - Bioengineering 2022Quote: ... a C-terminal gp160 trimerization domain and SnoopTagJr) were expressed in suspension Expi293F (Thermo Fisher) and ExpiCHO-S cells respectively and purified as described above for DogCatcher-RBD.
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bioRxiv - Neuroscience 2020Quote: ... mouse monoclonal antibody generated against the C-terminal domain of N-cadherin (Invitrogen; 1:500); anti-CRB1 (a kind gift of Dr ...
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bioRxiv - Genomics 2021Quote: ... and HLA-DR/human MHC class II antibodies (Invitrogen Dynabeads® ...
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bioRxiv - Immunology 2020Quote: ... for BALC/c cohorts and IgG (Total) Human Uncoated ELISA Kit (Invitrogen, #88-50550-22) for Hu-mice cohorts according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... and labelled with the GeneChip WT Terminal Labeling Kit (ThermoFisher Scientific) as per manufacturer’s instructions ...