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Citations for Thermo Fisher :
101 - 150 of 10000+ citations for Human KRT12 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The human Rab6a coding sequence (DNASU Plasmid Repository, #HsCD00296778, NCBI Nucleotide reference BC096818) was inserted by Gateway recombination (Invitrogen) to make an in-frame EmGFP-Rab6a fusion.
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bioRxiv - Cell Biology 2020Quote: ... Targeting sequence of SEPT9 shRNA was designed using BLOCK-iT™ RNAi Designer (Thermofisher) and the sequence was scrambled using the Invivogen shRNA wizard.
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bioRxiv - Developmental Biology 2022Quote: ... and FOXA2 shRNA knockdowns VFG culture was fixed in 1% formaldehyde (Thermo Scientific, 28906) in VFG media for 10 minutes at room temperature followed by a 5-minute quench with glycine (in True MicroChIP kit ...
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bioRxiv - Neuroscience 2021Quote: ... A control vector with scrambled non-silencing shRNA 59 was obtained from Thermo Scientific, and the shRNA to knock-down plasmid for mouse eIF4EBP1 was obtained from Sigma Aldrich (TRCN0000335381).
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bioRxiv - Microbiology 2023Quote: ... and 100ng of the TMER or shRNA vector was transfected with Lipofectamine 2000 (Thermofisher). After 48 hours the efficiency was checked visually by GFP control plasmid transfection ...
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bioRxiv - Genomics 2024Quote: ... and from SW480 cells stably expressing Ctrl or TOP1 shRNAs using TRIzol reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: A stable β2AR-overexpressing cell line was produced by transfecting CHO-K1 cells (ECACC) with a plasmid coding for the human β2AR with C-terminal GFP using lipofectamine 2000 (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... The plasmid was transfected into human osteosarcoma U-2 (U2OS) cells by electroporation using the Neon Transfection System (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... the ST1 cells were transfected with NICD pCMV Neo/intracellular domain of human Notch1 (NIC-1) plasmid using Lipofectamine 200 (Invitrogen), and then were harvested 48 hours after transfection for western blot and in vitro angiogenesis assay.
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bioRxiv - Microbiology 2020Quote: ... cells were transfected with an expression plasmid encoding human ACE2 - C-terminally fused to the GFP - using Lipofectamine 2000 (Invitrogen). Two days post transfection ...
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bioRxiv - Cell Biology 2022Quote: ... the pFastBac plasmid containing full-length human dynein or tail-truncated dynein was transformed into DH10Bac competent cells (Gibco, #10361012) with heat shock at 42 °C for 45 seconds followed by incubation at 37 °C for 4 hours in S.O.C ...
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bioRxiv - Molecular Biology 2022Quote: ... and 24 hours later cells were transfected with the Bluescript vector (empty vector control) and Human L1 plasmid (used in L1 retrotransposition assay) using Lipofectamine 2000 reagent (Invitrogen). Cells were harvested 36 hours after transfection ...
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bioRxiv - Genetics 2023Quote: ... The donor template for homologous recombination was generated by PCR amplification of a human DNA sample carrying 51 CAG repeat into the pCR-Blunt II TOPO plasmid (Invitrogen). Subsequently ...
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bioRxiv - Microbiology 2023Quote: ... The Pro-Pol fusion HA-tagged (ProWTPolWT and ProMUTPolWT) plasmids were generated using an in-house human PGK promoter-driven lentiviral vector via Gateway cloning (Invitrogen). Plasmids expressing HuSaV (GI.2 ...
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bioRxiv - Developmental Biology 2019Quote: Five shRNAs (Table S1) targeting to porcine Bmp15 mRNA was designed and selected by Invitrogen’s web-based siRNA design software (https://rnaidesigner.invitrogen.com/rnaiexpress/) ...
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bioRxiv - Molecular Biology 2019Quote: Short hairpin RNA (shRNA) sequences specifically targeting circE7 back-splice junction were designed from Invitrogen BLOCK-ItTM RNAi Designer (https://rnaidesigner.thermofisher.com/rnaiexpress/) ...
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bioRxiv - Molecular Biology 2019Quote: ... Media for HeLa1.2.11 and HCT116 shRNA knockdown cells also contained puromycin (1 μg/mL, Gibco) to maintain selection (Stewart et al. ...
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bioRxiv - Cell Biology 2020Quote: ... The corresponding direct and reverse oligos for every shRNA (Invitrogen; Thermo Fisher Scientific, Waltham, USA) were annealed and subcloned on a MLP plasmid vector which contains a green fluorescent protein sequence (GFP ...
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bioRxiv - Neuroscience 2021Quote: ... The shRNA sequence was selected using BLOCK-iT™ RNAi Designer provided by Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: NHE6-GFP in FuGW and shRNA constructs were transfected using lipofectamine 3000 (Invitrogen, Carlsbad, CA) into HEK293-FT cells to be packaged into virus using pCMV-Δ8.9 and PMDG at a ratio of 9:8:1 ...
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bioRxiv - Neuroscience 2023Quote: ... 1 μg of corresponding shRNA vectors were transfected into the cells using Lipofectamine 3000 (Invitrogen). Cells were harvested 48 hours after transfection for mRNA extraction using the RNAeasy mini plus kit (Qiagen) ...
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Transcriptional Isoforms of NAD+ Kinase regulate oxidative stress resistance and melanoma metastasisbioRxiv - Cancer Biology 2023Quote: miR-E shRNA knockdown constructs were generated by the splashRNA program and ordered from Invitrogen as oligos ...
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bioRxiv - Cancer Biology 2023Quote: miR-E shRNA knockdown constructs were generated by the splashRNA program and ordered from Invitrogen as oligos ...
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bioRxiv - Cancer Biology 2024Quote: ... Selection for shRNA transduced cells was performed using 2 µg/ml Puromycin DiHCL (Thermo Scientific).
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bioRxiv - Microbiology 2024Quote: ... and human (goat anti-human IgG, ThermoFisher) were also used ...
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bioRxiv - Biophysics 2020Quote: The DNA sequences of the human CE GTase and each sequence variant were synthesised and subcloned into the PGEX6p1-C-His plasmid vector by Thermo Fisher GeneArt ...
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bioRxiv - Cell Biology 2019Quote: ... we transfected one 100 mm plate of DLD1 BRCA2-/- cells at 70% of confluence with 10 µg of a plasmid containing human EGFP-MBP-tagged BRCA2 cDNA (corresponding to accession number NM_000059) using TurboFect (Thermo Fisher Scientific), 48h post-transfection the cells were serial diluted and cultured in media containing 1 mg/ml G418 (Sigma-Aldrich ...
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bioRxiv - Systems Biology 2019Quote: DNA encoding the SNAP-Tag was PCR-amplified from the pSNAPf vector (NEBs, #N9183S) to be inserted in frame into the pT7CFE1-CHis plasmid provided in the human IVT kit (ThermoFisher, #88882). The Gateway cloning cassette was introduced immediately after the SNAP tag sequence using the Gateway vector conversion system (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... The resulting plasmid DNA construct was used to transfect Expi293F human cells using the Expi293F transfection kit and Expi293F expression medium (Thermo Fisher). Cells were harvested by centrifugation at 1,000 g for 30 min and resuspended in 1 x PBS supplemented with 30 mM imidazole ...
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bioRxiv - Cancer Biology 2020Quote: ... envelope and one of the transfer plasmids were co-transfected into human embryonic kidney (HEK 293T) cells using Lipofectamine 3000 (ThermoFisher Scientific) according to the manufacturer’s lentiviral production protocol ...
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bioRxiv - Cell Biology 2020Quote: C2C12 myoblasts were transfected with a plasmid containing the N-terminal 88 amino acids of human cyclin B1 fused to EGFP using Lipofectamine 3000 (Invitrogen, L3000001) with the P3000 reagent according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Lenti-X Human Embryonic Kidney (HEK) cells were transfected with the sgRNA plasmids using Lipofectamine 3000 (Life Technologies, Cat. No. L3000150), then cultured for 2-3 days in the following media ...
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bioRxiv - Immunology 2021Quote: ... HEK293T cells were transfected with plasmid encoding Myc-tagged human NLRP3 using the Lipofectamine™ 3000 transfection kit (L3000015, Thermo fisher). At 24 h after transfection ...
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bioRxiv - Cell Biology 2023Quote: Methods used for these assays have been described.28 Briefly, human FLAG-tagged CDKL5 WT or kinase dead (KD, CDKL5 K42R) constructs were subcloned into pT7CFE1-CHis plasmid (Thermo Fisher). These constructs were then used for in vitro translation using a HeLa cell lysate-based Kit (1-Step Human Coupled IVT Kit—DNA ...
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bioRxiv - Immunology 2023Quote: ... Plasmids encoding paired Ig heavy and light chains were co-transfected into human embryonic kidney (HEK) 293F cells (Thermo Fisher Scientific) for recombinant mAb production ...
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bioRxiv - Neuroscience 2023Quote: ... FLAG-tagged WT or kinase dead (KD, CDKL5 K42R) constructs of human CDKL5 were subcloned into a pT7CFE1-CHis plasmid (Thermo Fisher). A HeLa cell lysate-based Kit (1-Step Human Coupled IVT Kit—DNA ...
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bioRxiv - Genetics 2024Quote: Flag-tagged wild type and mutated human RBBP5 plasmid were transfected to HEK293 cells by lipofectamine 3000 according to manufacturer’s protocol (Invitrogen, CA, USA). Cells were lysed by RIPA buffer and 20μg of whole cell lysate were used to assess protein expression in Western blot ...
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bioRxiv - Cancer Biology 2019Quote: ... Stable transfections of NIH – vGPCR for different shRNAs were performed using the Lipofectamine Plus Reagent (Invitrogen). NIH-vGPCR cells were plated at 60% confluence in 10 cm plates and transfected with 2ug of shRNA (shScramble and shRNAs targeted against Nrf2 - GIPZ Nfe2l2 shRNA Thermo RMM4532-EG18024) ...
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bioRxiv - Biochemistry 2021Quote: ... adenoviruses expressing LacZ and Tpi shRNA were cloned into the Adenoviral Gateway Expression System (Invitrogen, CA). In brief ...
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bioRxiv - Genomics 2021Quote: ... The WTC11 iPSCs transduced with shRNA lentivirus were treated with hygromycin (Gibco, 10687010, 150 µg/mL) for 7 days and then collected for RNA extraction and qPCR.
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bioRxiv - Evolutionary Biology 2022Quote: ... and shRNA for TP53 into 300,000 fibroblasts from each individual with a Neon Electroporation Device (Invitrogen), using a 100 μL kit ...
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bioRxiv - Cell Biology 2023Quote: ... Stable clones expressing the specific shRNAs were selected using 5μg/ml of puromycin (Gibco-life technologies) and pooled to generate the cells used in the experiments.
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bioRxiv - Cell Biology 2023Quote: ... Selection of stably shRNA-expressing polyclonal cell lines was achieved using puromycin (1 µg/mL; Gibco). pLKO.1 with non-targeting sequence 5’-AATTGCCAGCTGGTTCCATCA-3’ was used to generate shRNA control (shCON ...
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bioRxiv - Pathology 2022Quote: ... human CTRP3 (Invitrogen, PA5-115061, rabbit anti-human), α-SMA-Cy3 (Sigma ...
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bioRxiv - Cancer Biology 2021Quote: ... human (Invitrogen). Cells were cultured in DMEM (Corning Cellgro 10-013-CV ...
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bioRxiv - Cell Biology 2020Quote: The pCS2 Lrp6-APEX2-IRES-GFP-PAC plasmid was transfected into Human Embryonic Kidney 293T (HEK293T) cells using Lipofectamine2000 (Thermo Fisher Sci.). 48 hours after transfection puromycin was added to the culture medium ...
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bioRxiv - Molecular Biology 2020Quote: The dual pri-miRNA expression plasmids were transfected to human embryonic kidney (HEK) 293 cells in 6-well plates using the Lipofectamine 3000 reagents (Thermo Fisher Scientific) following standard procedures ...
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bioRxiv - Systems Biology 2021Quote: ... 400 ng of the constructed plasmids containing the human gene transcriptional units (Table S9D) were linearized by digestion with NotI (FastDigest, Thermo Fisher Scientific) according to the manufacturer’s protocol for 30 min and subsequently the digestion mix was directly transformed to IMX1076 ...
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bioRxiv - Biophysics 2020Quote: ... while hNAA25 contained a Tobacco-etch virus (TEV)-cleavable N-terminal 6xHis-tag. Human NatB complex (hNAA201-163/hNAA25FL) was obtained by coexpressing these two plasmids in Sf9 (S. frugiperda) cells (ThermoFisher, cat# 12659017), and purified as described previously[35] ...
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bioRxiv - Cancer Biology 2021Quote: ... together with 0.5µg of a plasmid expressing the renilla luciferase under the control of the human β-actin promoter using Lipofectamine 3000 (ThermoFisher Scientific) according to manufacturer’s protocol ...