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Citations for Thermo Fisher :
201 - 250 of 10000+ citations for Human Chemokine C X C Motif Receptor 5 CXCR5 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... antibodies overnight at 4°C and then incubated for an additional 3 hours at 4°C with 50 μL DynabeadsTM protein G (Life Technologies, 10004D). Following three washes with 1% Triton-TBS lysis buffer ...
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bioRxiv - Biochemistry 2021Quote: ... and protein was quantified in the supernatant for temperatures 37°C and 41°C by micro BCA assay (Thermo Fisher Scientific - Pierce). Following quantification ...
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bioRxiv - Cancer Biology 2021Quote: ... and protein was quantified in the supernatant for temperatures 37°C and 41°C by micro-BCA assay (Thermo Fisher Scientific - Pierce). Following quantification ...
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bioRxiv - Cancer Biology 2020Quote: ... or Insulin Receptor beta (ab69508) overnight at 4°C followed by anti-rabbit secondary antibody conjugated to horseradish peroxidase (ThermoFisher Scientific). Proteins were detected by Luminata Crescendo immunoblot HRP substrate (WBLUR0500Millipore Corporation ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... SNAP-tagged human β2-adrenoceptor or human EP2 receptors fused at the C terminus to a thermostable Nanoluciferase (Hek-ssβ2-AR-tsNluc) using Lipofectamine 3000 reagent (Invitrogen, Paisley, UK). Original SNAP tag and Nanoluciferase sequences were from NEB (Hitchen UK ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were then incubated overnight at 4°C with a 1:100 dilution of leptin receptor antibody (cat#PA1-053, ThermoFisher Scientific) in blocking buffer ...
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bioRxiv - Microbiology 2020Quote: ... cultivated at 37°C/5% CO2 with DMEM/F-12+Glutamax (Gibco) and 1% PIS ...
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bioRxiv - Developmental Biology 2019Quote: ... 5% dialyzed fetal bovine serum (FBS) (GIBCO ref. 26400-036; −20°C) and 1% of a 10000 U/ml stock solution of Penicillin/Streptomycin (GIBCO ref ...
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bioRxiv - Genomics 2019Quote: ... fragmented for 5 min at 37°C with 40U RNase I (Ambion), centrifuged at 15k RPM for 3 minutes at 4°C (with supernatant kept ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were cultured at 37°C and 5% CO2 in DMEM (Gibco; high glucose ...
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bioRxiv - Neuroscience 2020Quote: ... and cultured at 37°C in 5% CO2 in Neurobasal medium (Gibco) containing 2% B-27 supplement (Gibco) ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were cultured at 37°C with 5% CO2 on dishes (Nunc) coated with 0.1% gelatin (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... Nicola and cultured at 37 °C/5% CO2 in DMEM (ThermoFisher 11965) supplemented with 10% FBS (Atlas BiologicalsFS-0500-A ...
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bioRxiv - Molecular Biology 2021Quote: ... at 33 °C with 5% (v/v) CO2 in DMEM/F12 (Gibco) supplemented with 5 μg/mL insulin ...
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bioRxiv - Immunology 2022Quote: ... Cells were cultured at 37°C with 5% CO2 in DMEM (GIBCO) plus 10% heat-inactivated FCS with penicillin ...
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bioRxiv - Microbiology 2021Quote: ... at 37°C in a humidified 5% CO2 incubator (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2019Quote: ... Nicola and cultured at 37 °C/5% CO2 in DMEM (ThermoFisher 11965) supplemented with 10% FBS (Atlas BiologicalsFS-0500-A ...
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Mechanotransduction-dependent control of stereocilia dimensions and row identity in inner hair cellsbioRxiv - Cell Biology 2019Quote: ... Explants were cultured at 37°C and 5% CO2 in DMEM (Invitrogen) supplemented with 7% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2022Quote: ... Islets were cultured overnight (37°C, 5% CO2) in RPMI1640 medium (Thermofisher) with 11 mM glucose (Sigma) ...
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bioRxiv - Molecular Biology 2022Quote: ... for 5 minutes at 37°C and then with RNase A (ThermoFisher) for 5 minutes at 37°C ...
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bioRxiv - Cell Biology 2022Quote: Cells were cultured at 37°C with 5% CO2 in DMEM (Gibco) supplemented with 10% FCS and 1% penicillin-streptomycin-glutamine (Gibco).
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bioRxiv - Neuroscience 2023Quote: ... Cultures were grown at 37°C and 5% CO2 in Neurobasal (Invitrogen) supplemented with 2% B-27 (Invitrogen) ...
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bioRxiv - Molecular Biology 2022Quote: MEFs were maintained at 37°C (5% CO2) in DMEM media (GIBCO) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2023Quote: ... Nicola and cultured at 37 °C/5% CO2 in DMEM (ThermoFisher 11965) supplemented with 10% FBS (Atlas Biologicals FS-0500-A ...
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bioRxiv - Immunology 2022Quote: ... and cultured at 37 °C in 5% CO2 in RPMI 1640 (Gibco) supplemented with 10% FBS (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... and cultured at 37 °C in 5% CO2 in RPMI 1640 (Gibco,) supplemented with 20% FBS (Gibco,) ...
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bioRxiv - Developmental Biology 2023Quote: ... and trypsinizing for 5 minutes at 37 C (TrypLE Express, Gibco, 12605028). Trypsin was quenched with 2i + LIF media ...
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bioRxiv - Genomics 2023Quote: ... at 37°C with 5% CO2 (all products from Thermo Fisher Scientific). The differentiation medium was changed every day and embryoid bodies were collected by centrifugation for 4 minutes at 700 rpm and resuspended in 10ml of fresh medium.
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bioRxiv - Microbiology 2024Quote: ... MD) were cultured at 37 °C and 5% CO2 in DMEM (Gibco) supplemented with 10% fetal calf serum ...
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bioRxiv - Immunology 2024Quote: ... and labelled with CellTrace Violet (5 μM, 20 min, 37 °C, Invitrogen). Labelled CD4+ T-cells were co-cultured (4 d ...
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bioRxiv - Cell Biology 2023Quote: ... 85°C for 5 minutes (High Capacity cDNA Reverse Transcription Kit, ThermoFisher). Following conversion ...
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bioRxiv - Immunology 2024Quote: ... 5%CO2 and 37°C in Hera Cell Vios 160i incubators (ThermoFisher). In vivo inoculation occurred between 4-6 passages.
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bioRxiv - Cell Biology 2024Quote: ... Sections were blocked in 5% normal serum (Thermo Fisher Scientific, 10000 C) in PBS-T (0.4% Triton X-100 in PBS ...
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bioRxiv - Biophysics 2024Quote: HEK293T were cultured at 37° C and 5% CO2 in DMEM (Gibco) supplemented with 10% FBS (Fetal bovine serum ...
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bioRxiv - Microbiology 2020Quote: ... 5% CO2 and 5% O2 at 37°C in RPMI 1640 containing AlbuMAXII (Thermo Fisher Scientific) supplemented with 2 mM L-glutamine ...
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bioRxiv - Physiology 2022Quote: ... plasma C-reactive protein (CRP) was measured using an ELISA (Invitrogen, EM20RB). The ELISA was performed according to the manufactures protocol and the results were measured using a SpectraMax iD3 microplate reader (OD 450 nm) ...
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bioRxiv - Immunology 2021Quote: ... and anti-human AlexaFluor 647 (1:500, 30 minutes, 4°C; Life Technologies, A21445), and resuspended in CellFix (1:10 in water ...
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bioRxiv - Biochemistry 2020Quote: ... The protein with the C-terminal Fc-fusion was affinity purified with protein-A sepharose (Invitrogen). Samples were eluted with 0.1 M glycine pH 3.0 directly to a neutralizing buffer ...
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bioRxiv - Biochemistry 2021Quote: ... and an Acclaim PepMap C-18 column (50 cm x 75 μm, Thermo Fisher Scientific) coupled to a Q Exactive HF-X hybrid quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... 5 μg of FGFb Recombinant Human Protein and 1% Gluta-MaxTM (Gibco) in flasks coated with Matrigel Matrix Basement Membrane (Corning ...
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bioRxiv - Neuroscience 2023Quote: ... which specifically targets on the phosphorylated Thr188 of murine C/EBPβ (a conservative locus to Thr235 of human C/EBPβ) in eBioscience™ Transcription Factor Staining Buffer Set (Invitrogen, Catalog #00-5523-00). For background signal staining ...
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bioRxiv - Molecular Biology 2020Quote: SEC fractions in loading buffer were heated to 95°C for 5 min and separated alongside a spectra multi-color protein ladder (Thermo Fisher 26634) on a 4-15% Tris-Glycine extended Stain-Free gel (Bio-Rad 5678085 ...
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bioRxiv - Immunology 2020Quote: ... USA) at 95°C for 5 min and then mixed with 2μL of 5mg/mL Truecut Cas9 protein (Thermo Fisher Scientific, USA) for 10 min at 37°C in a total volume of 50μL ...
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bioRxiv - Molecular Biology 2021Quote: ... incubating at 95°C for 5 min and then mixed with 2μL of 5mg/mL Truecut Cas9 protein (Thermo Fisher Scientific, USA) for 10 min at 37° ...
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bioRxiv - Cancer Biology 2021Quote: ... and NCI-H23 cells (human NSCLC) were cultivated at 37°C with 5 % CO2 in RPMI 1640 medium (Gibco, Thermo Fisher Scientific, California, USA) supplemented with 10 % fetal calf serum (FCS ...
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bioRxiv - Cancer Biology 2021Quote: ... the sections were washed 3 times for 5 minutes and incubated overnight at 4 °C with ∼100ul primary antibodies diluted in 1% BSA+Triton-X (Thermo Fisher Scientific, Rockford, IL, USA). The following morning ...
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bioRxiv - Neuroscience 2022Quote: ... brain slices were washed (5 min at RT x 3 times) in PBS and incubated (overnight at 4°C) with streptavidin-Alexa 488 (ThermoFisher Scientific, S-11223, 1:500) in PBS-triton 0.05% ...
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bioRxiv - Molecular Biology 2023Quote: ... washed twice in PBS and after centrifugation at 250 x g for 5 min at 4°C resuspended in cell lysing buffer (Cell Signaling Cat# 9803, containing protease inhibitor, Thermo Fisher Scientific, Cat# 78415). Cell lysates were incubated on ice for 45 min with occasional mixing ...
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bioRxiv - Genomics 2019Quote: ... Samples were heat-denatured (95°C/ 5 min) and incubated o/n at 37°C with 150 U of λ-exonuclease (λ-exo; Thermo Scientific) in the presence of 40 U of RNAseOUT ...
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bioRxiv - Developmental Biology 2022Quote: ... 95 °C for 5 seconds and 60 °C for 20 seconds in a QuantStudio 6 Flex Real-Time PCR machine (Thermo Fisher Scientific, USA). The detectors used were FAM-BHQ1 and HEX- BHQ1 ...