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Citations for Thermo Fisher :
151 - 200 of 10000+ citations for Human Chemokine C X C Motif Receptor 5 CXCR5 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Duplicate wells of 200,000 CFSE-stained cells per sample were then incubated for 91 hours at 37°C (5% CO2) at a 1:2 bead-cell ratio with Human T-Activator CD3/CD28 DynaBeadsTM (Thermo Fisher, #11161D) and 1 U/mL of recombinant human IL-2 (BioLegend ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were cultured at 37°C/5% CO2 in DMEM (Gibco) supplemented with 10% FBS (Gibco ...
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bioRxiv - Bioengineering 2019Quote: ... were cultured in 5% CO2 at 37 °C in DMEM (Gibco) supplemented with 10% fetal bovine serum (Thermofisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... were cultured at 37°C and 5% CO2 in MEM (Gibco) supplemented with 1 mM of sodium pyruvate ...
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bioRxiv - Developmental Biology 2022Quote: ... and 5 μl of APC-conjugated anti-c-KIT (Invitrogen, CD11705) antibodies for 20 minutes at room temperature with rotation at 10 revolutions per minutes (rpm ...
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bioRxiv - Cell Biology 2023Quote: ... 5% CO2 and 37°C in DMEM (Thermo Fisher Scientific #10567022) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Molecular Biology 2024Quote: ... in a an incubator (37°C, 5% CO2, Thermo Fisher Scientific) for 14 hours to induce ex vivo ovulation ...
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bioRxiv - Immunology 2022Quote: ... CXCR5-Pe-Vio770 (MU5UBEE, Thermo Fisher Scientific), FOXP3-PerCP-Vio770 (PCH101 ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were pelleted down by centrifugation (10628 g at 25°C for 5 min, Thermo Fisher SCIENTIFIC #F9-6 x 1000 LEX rotor). Cells were washed once with water and suspended into 75 mL ice cold water ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were pelleted down by centrifugation (10628 g at 25°C for 5 min, Thermo Fisher SCIENTIFIC #F9-6 x 1000 LEX rotor). The cells were washed once with sterilized water and re-suspended into 6 L MM composed of 1.34% yeast nitrogen base without amino acids (SIGMA Y0626) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the reaction tubes were centrifuged for 5 min at 4 °C and 23000 rpm (approx. 49000 x g, Heraeus Biofuge Stratos, rotor 3331, Thermo Scientific, Waltham, USA). In the next step ...
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bioRxiv - Cell Biology 2021Quote: ... S1 C (Pierce Unstained Protein MW Marker from Thermo Scientific, 26610) and Fig ...
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bioRxiv - Biochemistry 2021Quote: ... immobilized on Dynabeads Protein A magnetic beads (Thermofisher, c/n 10001D) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were immobilized overnight at 4°C on Maxisorb™ (Nunc) 96 wells microplates at 1µg/well in 100 µl NaHCO3 0.1 M pH 8.9 ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... isolated with Protein A Dynabeads (Invitrogen; 4 h at 4°C), washed thrice with RSB+T ...
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bioRxiv - Cell Biology 2024Quote: ... Cell lysates were clarified by centrifugation for 15 min (21.000 x g and 4 °C) and protein content in the supernatant was determined using a Pierce™ BCA assay kit (Thermo Scientific, USA). Cell lysates were diluted to a final concentration of 2 mg protein/ ml in native lysis buffer and kept on ice until used freshly.
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bioRxiv - Neuroscience 2024Quote: ... Protein samples were concentrated on a trap column (PepMap C18, 5 mm x 300 μm x 5 μm, 100Ǻ, Thermo Fisher Scientific) with 2:98 (v/v ...
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bioRxiv - Immunology 2020Quote: Up to 5×106 isolated cells were incubated for 30 min at 4 °C with anti-CD16/CD32 (Fc receptor) clone 93 (Invitrogen) to block non-specific binding and with fixable viability dye eFluor455UV (eBioscience ...
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bioRxiv - Biochemistry 2023Quote: ... 155-238) were fused to the C-termini of tagged receptors and cloned into the NheI-XhoI sites of pCEP4 (Invitrogen). Plasmids for HIV-1 MN gp160 and BaL gp160 (# 100919 ...
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bioRxiv - Molecular Biology 2023Quote: ... and 5 ng/uL human IL-6 recombinant protein (ThermoFisher PHC0066).
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bioRxiv - Genomics 2021Quote: ... 30 s at 60°C and 30 s at 72°C and a final extension at 72°C for 5 min (performed on a Mastercycler Nexus Gradient (Thermofisher Scientific). Amplicons underwent a second round of PCR to attach flow cell adapters and unique barcodes ...
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bioRxiv - Neuroscience 2022Quote: ... Reverse transcription was performed as follows: 53°C for 45 minutes and 85°C for 5 minutes in a Veriti Therml Cycler (Applied Biosystems). Next ...
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bioRxiv - Immunology 2021Quote: ... (originally isolated from male BALB/c mice) were cultured at 37°C with a humidified atmosphere of 5% CO2 in DMEM (Thermo Fisher) with 10% FBS (Sigma Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... Control RNAi experiments were performed either using siGENOME lamin A/C Control (ThermoFisher Scientific: Fig. 5 B-C, S3 A, B) or ON-TARGET-plus Non-targeting siRNA #1 (Thermo Scientific Dharmacon) ...
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bioRxiv - Biochemistry 2023Quote: ... The temperature was increased from 5 °C to 95 °C over 2 hours and readings taken using a One Step Plus RT-PCR (Applied Biosystems).
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bioRxiv - Cancer Biology 2023Quote: ... a final extension step (68°C for 5 min) and holding the reaction at 4°C in a thermocycler (Applied Biosystems) with a preheated lid (105°C) ...
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bioRxiv - Cell Biology 2024Quote: ... Two sets of 12 100 µL 2.66 ng/ µL enriched resuspended mitochondria aliquots in 200 µL PCR tubes were made per condition and heated on a temperature gradient of 42-64°C (2°C steps) for 5 minutes at each temperature using a Veriti 96-well thermal cycler (Applied Biosystems).
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bioRxiv - Biophysics 2022Quote: Samples were heated at 95°C for 5 minutes before loading the onto the PAA-gel (marker: PageRuler Plus Prestained protein ladder (Thermo Scientific)) ...
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bioRxiv - Cancer Biology 2021Quote: ... The samples were then boiled at 100 °C for 10 min and 5 μl of each sample was used for protein quantification using the MicroBCA assay (Thermo Scientific) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Thawed cell pellets were heated 5 min at 95°C before measuring protein contend by using a micro-BCA assay (Pierce, ThermoFisher Scientific) and a NanoDrop One spectrophometer (ThermoFisher Scientific).
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bioRxiv - Molecular Biology 2022Quote: ... then the supernatant was diluted 10x and pre-cleared for 30 min at 4 °C with rotation using 5 µl protein A- and G-coupled dynabeads (ThermoFisher Scientific). An input sample was taken ...
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bioRxiv - Microbiology 2020Quote: ... Two and a half micro liter of pBAD-C+SPA+gst sample as well as 5 μL of Spectra Multicolor Low Range Protein Ladder (Thermo Scientific) and 10 μl of samples or controls were loaded on tricine-SDS-gels (Schägger ...
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bioRxiv - Biochemistry 2022Quote: MT-4 cells with and without lentiviral plasmids were collected by centrifugation (225× g, 5 min, at 20 °C) and proteins were extracted using lysis buffer (RIPA Thermo Scientific, cOmplete™ ...
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bioRxiv - Immunology 2022Quote: ... 700 μg of protein were mixed and rotated with 0.5 μg of STING antibody for 3 h at 4 °C after which 25 μL of Dynabeads Protein G (Invitrogen, 10004D) were added to each sample and rotated for an additional hour ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were heated at 95°C for 5 min and proteins resolved on 4-20% acrylamide Tris-glycine gradient gels (Life Technologies). Proteins were then transferred to polyvinylidene difluoride membranes (EMD Millipore ...
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bioRxiv - Biophysics 2023Quote: ... heated to 95 °C for 5 min and loaded onto the gel with PageRuler™ Prestained Protein Ladder (Thermo Fisher Scientific) as a reference marker ...
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bioRxiv - Neuroscience 2023Quote: ... centrifuged at 300g for 5 minutes at 4°C and homogenized in cold N-PER Neuronal Protein Extraction Reagent (Thermo Fisher) or cold RIPA lysis buffer (Thermo Fisher ...
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bioRxiv - Molecular Biology 2024Quote: ... cell extracts and purified protein samples were boiled at 95°C for 5 minutes followed by fractionation on NuPAGE Bis-Tris polyacrylamide gels (Thermo Scientific). Separated proteins were transferred to Immobilon FL polyvinylidene difluoride (PVDF ...
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bioRxiv - Immunology 2024Quote: ... Lysates were then incubated overnight at 4 °C with 5 μg of prebound anti-SP100 or rabbit IgG isotype control protein A dynabeads (Thermo Fisher). The next morning ...
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bioRxiv - Immunology 2021Quote: ... for each field.15 Analysis of cytokine secretion in primary human AML samples was performed using the Cytokine/Chemokine/Growth Factor 45-Plex Human ProcartaPlex™ system (ThermoFisher).
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bioRxiv - Molecular Biology 2023Quote: ... The cells stably expressing human recombinant LOXL1 were maintained in culture (37°C, 5% CO2) at log-phase growth in advanced DMEM (Thermo Fisher Scientific, 12491015) with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were pelleted down by centrifugation (10628 g at 25°C for 5 min, F9-6 x 1000 LEX rotor, Thermo Fisher Scientific, Waltham, MA). The cells were washed once with sterilized water and re-suspended into 6 L MM composed of 1.34% yeast nitrogen base without amino acids (SIGMA Y0626) ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were pelleted down by centrifugation (10628 g at 25°C for 5 min, F9-6 x 1000 LEX rotor, Thermo Fisher Scientific, Waltham, MA). Cells were washed once with water and suspended in 75 mL of ice-cold water ...
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bioRxiv - Biochemistry 2023Quote: ... 0.1% FA and injected onto an Acclaim PepMap100 C-18 trap column (5 µm, 100 µm x 20 mm, Thermo Scientific, Sunnyvale, CA, USA) for sample desalting ...
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bioRxiv - Cell Biology 2021Quote: ... with or without inhibitors at 4°C for 5 min and 37°C for 30 min in the presence of FM4-64FX (Thermo Fisher Scientific) to stain wounded cells ...
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bioRxiv - Neuroscience 2022Quote: ... 72°C for 5 minutes and then held at 4°C in a Veriti™ 96-well thermal cycler (Applied Biosystems™). Adaptors from the LSK-109 sequencing kit (Nanopore ...
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bioRxiv - Molecular Biology 2022Quote: ... 95°C for 20sec then 40 cycles of 95°C for 3sec and 58°C for 45sec on the QuantStudio 5 and 7 qRT-PCR systems (Applied Biosystems, CA). A known negative sample and non-template control (NTC ...
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bioRxiv - Neuroscience 2022Quote: ... supplemented with 5% Fetal Bovine Serum (FBS), 4°C) and incubated (37°C, 2 minutes) in protease solution (TrypLE express, Thermo Fisher 12604013) supplemented with DNase (100 U/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... The oligos were annealed in a thermocycler with gradual T reduction from 95°C to 25°C at a rate of 5°C/min and subsequently diluted 1:20 into Nuclease free water (ThermoFisher Scientific AM9938). The pLentiCRISPRv2 plasmid was digested for 1 hr at 55°C with 1U per μg of DNA BsmBI-v2 (NEB ...
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bioRxiv - Immunology 2023Quote: ... into the reaction mix and incubating it at 37 °C for 15 min and 98 °C for 5 min in the Veriti Thermal Cycler (Applied Biosystems, USA). Quantitative analysis of IL-6 and IFNB expression in THP1 dual reporter cells was performed by StepOne Real-Time PCR Systems (Thermo Fisher Scientific ...