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Citations for Thermo Fisher :
351 - 400 of 10000+ citations for HLA A*0201 HPV16 E7 complex Protein Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... FlpIn HEK293 cells were obtained from Thermo Fisher. Hras-/- ...
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bioRxiv - Systems Biology 2022Quote: The Flp-in T-REx HEK293 system (Invitrogen) was utilized for both stable plasmid transfection as well as doxycycline-induced expression ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293 cells were grown in DMEM (Life Technologies) with 10% FBS at 37 °C with 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: HEK293 cells (ATCC) were cultured in DMEM (Gibco) + 10% FBS (Seradigm) ...
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bioRxiv - Cell Biology 2023Quote: HEK293 cells were transfected with Lipofectamine200 (ThermoFisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: HEK293 cells were transiently transfected with Lipofectamine3000 (Invitrogen) according to the manufacturer’s protocols ...
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bioRxiv - Neuroscience 2023Quote: HEK293 cells were transfected with Lipofectamine 3000 (Invitrogen) according to the protocol of the manufacturer and incubated for 24 hours ...
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bioRxiv - Neuroscience 2023Quote: HEK293 cells were transfected using Lipofectamine 2000 (Invitrogen) and plasmid DNA purified with the Plasmid Plus Maxi Kit (Qiagen ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 cells were cultured in DMEM (Gibco, 11995065) + 10% fetal bovine serum (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: HEK293 cells were cultured in DMEM (Gibco-Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (FBS Invitrogen-Thermo ...
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bioRxiv - Biophysics 2022Quote: HEK293 cells were cultured in DMEM (Gibco-Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (FBS Invitrogen-Thermo ...
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bioRxiv - Neuroscience 2023Quote: The HEK293 Flp-InTM cell line (Invitrogen, R78007) was purchased from the Duke Cell Culture Facility ...
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bioRxiv - Molecular Biology 2023Quote: HEK293 cells were cultured in DMEM (Life Technologies), supplemented with 10% FBS (Life Technologies ...
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bioRxiv - Biochemistry 2024Quote: HEK293 cells (ATCC) were grown in DMEM (Gibco) with 10% FBS (Gibco ...
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bioRxiv - Immunology 2021Quote: RBD-His protein was expressed in Expi293 cells and purified on Ni-NTA resin (#88221, Thermo Fisher) followed by size-exclusion chromatography on a Superdex 200 gel filtration column in PBS.51 S1-S2-His (referred as S ...
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bioRxiv - Biochemistry 2022Quote: ... The total protein level was detected by primary anti-His-tag antibody (1:10000, MA1-21315, Invitrogen) and secondary anti-mouse antibodies (1:5000 ...
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bioRxiv - Immunology 2022Quote: For purification of the fusion proteins by His Pur™ Ni-NTA Resin (Thermo Scientific, Beijing, USA), the 250 mL IPTG-induced bacterial solution was centrifuged at 4 °C at 12000 rpm for 10 minutes ...
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bioRxiv - Microbiology 2022Quote: ... His-tagged proteins were purified using HisPur™ Ni-NTA resin (Thermo Fisher Scientific Cat. Nr. 88222) according to manufacturer’s instructions (batch protocol) ...
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bioRxiv - Biochemistry 2020Quote: ... The expression of the recombinant protein was measured by ELISA using anti‐His tag monoclonal antibody (Invitrogen) to capture and biotinylated anti‐ANG1 antibody for detection (R&D System) ...
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bioRxiv - Genetics 2021Quote: Plasmids expressing GST- and His-tagged proteins were transformed into BL21(DE3) competent cells (Thermo Scientific, EC0114) and grown in LB media at 37°C ...
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bioRxiv - Immunology 2022Quote: ... The His-tagged proteins were purified with the HisPur Ni-NTA Resin (Thermo Fisher Scientific cat.# 88221). After washing by wash buffer (25 mM Imidazole ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant AtCSD1 protein containing a 6xHis-tag was purified using Dynabeads His-Tag (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Eluted proteins were then incubated with Dynabeads His-Tag Isolation and Pulldown (Thermo Fisher Scientific, Waltham, Massachusetts) for 20 minutes and then washed 5 x 1 minute in His-tag Isolation Buffer (100 mM Na-phosphate ...
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bioRxiv - Immunology 2023Quote: ... Filtered supernatants containing His-tagged proteins were passed slowly through HisPur Ni-NTA Resin (Thermo Fisher 88221) beads in columns ...
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bioRxiv - Biochemistry 2023Quote: ... and proteins were visualized using Ponceau stain and Western blot with monoclonal anti-His (Invitrogen MA1-21315), and polyclonal anti-GST (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... 10% HI FBS (Gibco), 1X ultraglutamine (Lonza) ...
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bioRxiv - Microbiology 2021Quote: ... His (ThermoFisher, MA1-21315), FLAG (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... anti-His mAb (Invitrogen) and anti-GAPDH mAb (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... HI FBS (2273356P, Gibco). HEPES (54457 ...
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bioRxiv - Immunology 2020Quote: ... or anti-His (Invitrogen) for recombinant ZVrecE80 antigen in 0.1M carbonate buffer pH 9.6 ...
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bioRxiv - Immunology 2020Quote: ... + 10% HI-FCS (Gibco) + 1% Peniciliin/Streptomycin (100x ...
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bioRxiv - Microbiology 2022Quote: ... anti-penta HIS (Invitrogen) and anti-HSP70 (26 ...
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bioRxiv - Molecular Biology 2023Quote: ... 10% HI-FBS (Gibco), 1% penicillin/streptomycin (Gibco) ...
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bioRxiv - Molecular Biology 2023Quote: ... 10% HI-FBS (Gibco), 1% penicillin/streptomycin (Gibco) ...
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bioRxiv - Molecular Biology 2023Quote: ... 10% HI-FBS (Gibco), 1% penicillin/streptomycin (Gibco) ...
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bioRxiv - Cancer Biology 2023Quote: ... and His-tag (Invitrogen) were used ...
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bioRxiv - Immunology 2023Quote: Human-derived cell lines used in this study were initially obtained from accredited commercial suppliers: (1) HEK293 (human embryonic kidney cell line, FreeStyle® 293-F) was obtained from Invitrogen (San Diego, CA) for use as a recombinant protein expression vehicle ...
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bioRxiv - Genomics 2019Quote: ... 500 ng of in situ Hi-C libraries were first mixed with 2.5 μg human Cot-1 DNA (Invitrogen #15279011), 2.5 μg salmon sperm DNA (Invitrogen #15632011) ...
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bioRxiv - Bioengineering 2020Quote: ... T cells were cultured in RPMI-1640 supplemented with 10% HI-FBS and fed with recombinant human IL-2 (ThermoFisher) and IL-15 (Miltenyi ...
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bioRxiv - Immunology 2021Quote: ... The expression vectors encoding mHVEM (Q39-Q206) fused with human IgG1 and a subsequent hexa-His tag sequences were transfected into Expi293 (Gibco) cells using ExpiFectamine 293 transfection kit (Gibco ...
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bioRxiv - Immunology 2021Quote: ... The DNA sequences encoding a protein biologic composed of mHVEM residues (Q39- Q206) followed by human IgG1 and a subsequent hexa-His tag sequences were cloned into pcDNA 3.3 vector (Life technologies) using In-fusion HD cloning enzyme premix (Clontech) ...
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bioRxiv - Systems Biology 2023Quote: ... T cells were expanded in RPMI-1640 media supplemented with 10% heat-inactivated FBS (HI-FBS) as well as recombinant human IL-2 (50 U/mL; ThermoFisher) and IL-15 (1 ng/mL ...
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bioRxiv - Cancer Biology 2020Quote: ... The phage library was incubated with biotinylated CD40-Fc fusion protein or Her2 recombinant protein (Acro biosystems) for 2 hours at RT and the phage-antigen complex was captured by Dynabeads M280 (Life technologies). The bound phages were eluted by Glycin-HCl (pH 2.2 ...
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The Ets protein Pointed P1 represses Asense expression in type II neuroblasts by activating TaillessbioRxiv - Developmental Biology 2021Quote: ... Samples were incubated at 65°C overnight for reversing cross-linking of the chromatin–protein complex and degrading RNAs with 100µg/ml RNase A (Catalog # 12091021, Thermo Fisher Scientific), and then treated with 100µg/ml proteinase K at 45°C for 1hr ...
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bioRxiv - Bioengineering 2020Quote: Each gel electrophoresis reaction used 6 µL of unbound B56 protein-antibody complex and 2 µL of 4x SDS-PAGE sample buffer (Thermo Fisher). After heating the samples to 95 C for 10 minutes ...
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bioRxiv - Biophysics 2019Quote: ... HAUS8 (MT binding subunit of augmin complex)-and rabbit IgG(control)-antibody were conjugated to Protein A Dynabeads (Life Technologies #10002D) overnight ...
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bioRxiv - Genomics 2020Quote: ... We used 4 ug of antibody to H3K27ac and captured the chromatin-antibody complex with 34 uL Protein A beads (Thermo Fisher). Qubit quantification following ChIP ranged from 125-150 ng ...
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bioRxiv - Molecular Biology 2022Quote: ... The concentration of the Cas7-11–Csx29–crRNA complex was measured using Pierce 660-nm Protein Assay Reagent (Thermo Fisher Scientific). Csx30 was expressed in E ...
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bioRxiv - Developmental Biology 2022Quote: ... was added to the sonicated chromatin and incubated with the antibody-bead complex (Pierce Protein A/G Magnetic Beads, Thermo Scientific) overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... The immunoprecipitated RNA-antibody complex was captured by incubating with protein A and G magnetic beads (10002D, 10004D, Thermo Fisher Scientific) which were washed twice in RIP buffer ...