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Citations for Thermo Fisher :
251 - 300 of 10000+ citations for HLA A*0201 HPV16 E7 complex Protein Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... and anti-HLA-DR-PE-Texas Red (TU36, Invitrogen); Bmem ...
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bioRxiv - Microbiology 2023Quote: ... FITC labeled HLA-ABC Antibody (11-9983-42 Invitrogen), PE Mouse Anti-Human CD184 (Clone 12G5 ...
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bioRxiv - Immunology 2023Quote: ... Expression constructs for M35-V5/His and M27-V5/His (both in pcDNA3.1-V5/His, Invitrogen) have been described previously (Munks et al ...
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bioRxiv - Biophysics 2019Quote: Human embryonic kidney cells 293 (HEK293-6E, NRC, Canada) were cultured in FreeStyle F17 expression medium (Thermo Fisher Scientific, Waltham, MA) supplemented with 2 mM L-glutamine (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... N-terminal FLAG-tagged human MCC (P23508-1) constructs (pCMV2B vector) were transfected into HEK293 cells and selected with G418 (Gibco #10131035). G418-resistant cells were grown ...
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bioRxiv - Biophysics 2021Quote: Human wild type ABCG2 or ABCG2 R184A containing an N-terminal Flag-tag was expressed in HEK293-EBNA (Thermo Fisher Scientific) cells as previously described19 ...
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bioRxiv - Molecular Biology 2021Quote: ... the coding sequence encoding human TSPO (hTSPO) was amplified from cDNA previously generated by reverse transcription from total HEK293 mRNA isolated using Trizol (Life Technologies) and cloned into either a pENTR/SD/TOPO vector (Invitrogen ...
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bioRxiv - Genetics 2024Quote: Flag-tagged wild type and mutated human RBBP5 plasmid were transfected to HEK293 cells by lipofectamine 3000 according to manufacturer’s protocol (Invitrogen, CA, USA). Cells were lysed by RIPA buffer and 20μg of whole cell lysate were used to assess protein expression in Western blot ...
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bioRxiv - Microbiology 2023Quote: ... A portion of the Fc blocked-cells were separated to accommodate the unlabeled control while remaining samples were incubated with mouse anti-human HLA-ABC W6/32 (Invitrogen [MA5-11723], 1:40) on ice for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... for 2hours at 4°C and this was followed by precipitation of the protein-antibody complex with dynabeads protein G (Invitrogen, #300D, Carlsbad, CA, USA) for 2h in 4°C on rotator ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK293 and MS2-tagged HEK293:24xMS2-NEAT1 cells were cultured in Dulbecco’s modified eagle’s medium (DMEM, Gibco) with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2021Quote: Pooled peptide fractions eluted from an HLA class I sample and an HLA class II sample were analyzed on a Q Exactive mass spectrometer (Thermo Fisher Scientific, USA) coupled to an EASY-nLC 1000 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Aliquots of transfected cells were retained for validation of HLA-A2 expression by staining with HLA-A2 monoclonal antibody (BB7.2, Thermo Scientific, 17-9876-42) and subsequent flow cytometry analysis.
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bioRxiv - Cell Biology 2020Quote: Plasmids encoding for recombinant His-tagged proteins were transformed into Escherichia coli BL21(DE3) (Invitrogen). Bacterial cultures were grown at 37°C in LB media to OD600 of 0.6-0.8 and induced with 0.5 mM IPTG at 18 °C for 16h (overnight) ...
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bioRxiv - Cell Biology 2019Quote: ... The His-mCherry-fusion proteins were purified using a Ni-NTA column (Thermo Fisher Scientific). The eluted proteins were dialyzed in 1 L dialyzed buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: ... His-tagged proteins were visualised using a primary anti-6xhis antibody (ArtNr: 11533923, Fisher Scientific), a HRP-coupled secondary antibody (ArtNr ...
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bioRxiv - Neuroscience 2023Quote: ... The His-tagged dAsapPZA protein was expressed and purified following the manufacturer’s guidelines (Invitrogen, USA).
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bioRxiv - Bioengineering 2021Quote: The HHH peptide (Cys-biotin-PEG2-Gly-His-His-His-Gly(COOH)) was purchased from Thermo Fisher. It was 84% pure as determined by mass spectrometry ...
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bioRxiv - Microbiology 2020Quote: The full length CPSF6 protein was expressed in a suspension-adapted HEK293 cell line (Expi 293F, Thermo Fisher Scientific) by transfection of expression plasmid using ExpiFectamine 293 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... cell or nuclear lysates (400 ug) prepared from HEK293 or HDFs to Dynabeads Protein A (10001D, Thermo Fisher Scientific) that were prebound with 2–3μg of indicated antibody and incubated overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... SDHA (complex II) (1/1,000; Invitrogen), PRDM16 (1/1,000 ...
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bioRxiv - Molecular Biology 2024Quote: The cDNA sequence of human Sph1 was subcloned from the pcDNA3.1/V5-His-TOPO expression vector (Invitrogen, USA) 62 into the Venus-BiFC plasmids previously described 37 ...
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bioRxiv - Molecular Biology 2021Quote: ... ELMSAN1 TRERF1 DKO HEK293 cells were generated from wild-type Flp-In T-REx HEK293 cells (Invitrogen, R78007) by the Center for Advanced Genome Engineering (CAGE ...
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bioRxiv - Cancer Biology 2022Quote: ... (FBS HI; Invitrogen) and 1% Glutamax (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... anti-His (Invitrogen), or anti-FLAG (Sigma ...
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bioRxiv - Immunology 2022Quote: The Invitrogen ProtoArray® Human Protein Arrays (ThermoFisher Scientific) are high-density microarrays that contain more than 9,000 unique human proteins individually purified and arrayed onto a nitrocellulose-coated slide ...
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bioRxiv - Bioengineering 2023Quote: ... and recombinant human interleukin-2 (IL-2) protein (Invitrogen) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: HEK293 Flip-In TREx cells (Thermo Fisher) were cultured at 37°C with 5% CO2 in DMEM (Thermo-Fisher ...
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bioRxiv - Biochemistry 2020Quote: HEK293-F cells (FreeStyle™, ThermoFisher #R79007) were maintained in suspension culture according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... HEK293T or HEK293 GnTI- cells (Thermo Fisher) were incubated with 200 nM (monomer concentration ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK293 Flp-In T-REx 293 (Invitrogen), was grown at 37°C in DMEM high glucose supplemented with 5% Fetal Bovine Serum ...
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bioRxiv - Cell Biology 2022Quote: HEK293-Flp-In T-Rex cells (Invitrogen) were cultured in high glucose DMEM containing 10% FBS at 5% CO2 ...
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bioRxiv - Cell Biology 2021Quote: ... HEK293 cells were cultured in DMEM (Gibco) supplemented with 10% FBS and 1% penicillin/streptomycin ...
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bioRxiv - Cell Biology 2021Quote: HEK293 cells grown in DMEM media (Gibco) supplied with 10%FBS and 2mM L-Glutamine were seeded onto coverslips in 24 well plates and allowed to adhere and grow overnight at 37°C incubator supplied with 5% CO2 ...
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bioRxiv - Cell Biology 2021Quote: ... HEK293-FT cells were purchased from Invitrogen. U87MG cells were Purchased from the ATCC and cultured as previously described (Sabag et al. ...
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bioRxiv - Immunology 2020Quote: ... HEK293 cells was cultured in DMEM (Invitrogen) supplemented with 10% FBS at 37 °C and 5% CO2 environment ...
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bioRxiv - Cancer Biology 2022Quote: ... Flp-In T-REx HEK293 cells (ThermoFisher) were cultured in DMEM (Gibco) ...
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bioRxiv - Molecular Biology 2023Quote: Flp-In 293 cell line (HEK293; ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: HEK293 cells were maintained in DMEM (Gibco) supplemented with 10% FBS (Gibco ...
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bioRxiv - Genetics 2023Quote: HEK293 cells (Thermo Fisher, Waltham, MA, USA) were cultured at 37°C in Dulbecco’s Modified Eagle’s Medium containing high levels of glucose (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: HEK293 cells were grown in DMEM (Gibco) containing penicillin (100 U/mL ...
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bioRxiv - Genetics 2024Quote: ... HEK293 cells were cultured in DMEM (Gibco) supplemented with 10% FBS (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293 cells were grown in DMEM (Gibco) medium with 10% FBS ...
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bioRxiv - Microbiology 2024Quote: HEK293 cells were maintained in DMEM (Invitrogen) supplemented with 10% FBS (Cytiva) ...
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bioRxiv - Molecular Biology 2024Quote: Flp-InTM T-RexTM HEK293 (Thermo Fisher), Flp-InTM T-RexTM HeLa (gift from S ...
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bioRxiv - Immunology 2022Quote: ... and HLA-E (12-9953-42) were purchased from Thermofisher. For intracellular staining ...
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bioRxiv - Cancer Biology 2022Quote: ... Selected endogenous HLA-I ligands were ordered from Thermo Fisher Scientific as crude (PePotec grade 3 ...
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bioRxiv - Immunology 2023Quote: ... 3D12 (anti-HLA-E antibody ; Thermo Fisher # 4-9953-82) or the isotype-matched control Ab (Supplementary Figure 2).
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bioRxiv - Developmental Biology 2022Quote: ... ETC complex activity assays were normalized to cell lysate protein content via Bicinchoninic Acid (BCA) assay (Pierce, ThermoFisher Scientific) as per manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: ... The protein-antibody complex was developed by adding 2-3ml of SuperSignal West Pico Chemiluminiscent substrate (Thermo Fisher, USA).