Labshake search
Citations for Thermo Fisher :
601 - 650 of 6145 citations for GDF 11 BMP 11 Human HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2022Quote: ... for endothelial cells, anti-CD133 FITC (11-1339-42, eBioscience, San Diego, CA, USA) for stem cells and Hoechst 33342 (62249, Thermofisher Scientific, Waltham, MA, USA) for nuclei ...
-
bioRxiv - Immunology 2022Quote: Primary cells were cultured at 37°C and 10% CO2 in a humidified incubator in complete medium that consisted of DMEM medium (Gibco, Cat# 11-965-118) supplemented with 10% fetal bovine serum ...
-
bioRxiv - Microbiology 2021Quote: ... the peptide mixture was labeled with TMT 10-plex and 11-plex isobaric tandem mass tags (catalog no. 90110 and A34808, Thermo Fisher Scientific, Rockford, USA) following the manufacturer’s instruction ...
-
bioRxiv - Bioengineering 2021Quote: ... Each culture was divided into 12 wells of a 24-well plate (2ml of the diluted cultures) with a glass bead (Fisher Scientific, #11-312B 4mm) to increase aeration and the plates were covered with a breathable sealing membrane (USA Scientific #9123-6100 ...
-
bioRxiv - Microbiology 2021Quote: ... were prepared from 10 to 11-day-old chicken embryos (SPF eggs, VALO, Cuxhaven, Germany) using recombinant trypsin (Tryple TM, Thermo Fisher Scientific, Planegg, Germany) and maintained in VP-SFM medium ...
-
bioRxiv - Bioengineering 2021Quote: ... 200uL of the cell suspension was plated on YP-SUC plates and spread throughout the plate using glass beads (Fisher Scientific, #11-312B 4mm). The plates were wrapped in sterile ...
-
bioRxiv - Cancer Biology 2022Quote: ... were previously obtained73 from the Harmon Cancer Center Collection (University of Texas-Southwestern Medical Center) or commercially from the American Type Culture Collection (ATCC) and cultured in RPMI 1640 medium (Fisher Scientific, 11-875-119) supplemented with 5% FBS (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2023Quote: ... ratio and pERK:ERK (pT202/pY204, pT185/pY187) ratio was done using the AKT (Total/Phospho) InstantOne ELISA Kit (Thermo Fisher Scientific, 85-86046-11) for pAKT:AKT ...
-
bioRxiv - Immunology 2023Quote: We used FITC-conjugated anti-CD86 (11-0862-82) and PE-conjugated anti-MHCII (12-5320-82) antibodies from Thermo Fisher (Waltham, MA, USA) to stain the Mφ activation markers CD86 and MHCII ...
-
bioRxiv - Cell Biology 2022Quote: ... was formulated using a method slightly modified from a published report9: basal medium composed of 66.7% DMEM (high glucose, Thermo Fisher Scientific, 11-965-118) and 33.3% F12K (Thermo Fisher Scientific ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... incubated after for 2’ at RT with Stop Reagent (1:11, in 1XPBS, Alexa Fluor 488 Tyramide SuperBoost Kit, goat anti-rabbit IgG, Invitrogen, Thermo Fisher Scientific, Ghent, Belgium), washed three times in 1XPBS for 5’ each and incubated for 10’ at RT with Hoechst (1:1000 ...
-
bioRxiv - Immunology 2023Quote: ... the cultured B cells were stained with Fc block followed by fixable viability dye eFluor 780 and FITC BAFF-R monoclonal antibody (Invitrogen; cat #11-5943-81) and analyzed on a LSRFortessa X-20 flow cytometer (BD).
-
bioRxiv - Systems Biology 2024Quote: TMT labeling was performed according to our previously established dry TMT labeling workflow using an 11-plex TMT Mass Tagging Kit (Thermo Fisher Scientific, lot XB323735) 13 ...
-
bioRxiv - Biochemistry 2023Quote: ... Individual fractions 2-11 were analyzed by LC-MS using 55 min methods on the LTQ-Orbitrap Velos mass spectrometer (Thermo Fisher Scientific, Waltham, MA), and three pools of fractions (2-5 ...
-
bioRxiv - Molecular Biology 2023Quote: ... in FACS buffer (PBS with 2% BSA and 0.025% sodium azide): IgD (1:500, 405716 or Thermo Fisher 12-5993-82; 11-26c.2a), IgG1 (1:200 ...
-
bioRxiv - Cell Biology 2024Quote: ... 10% HIFBS) and reverse transfected with 1 pmol/well of a combination of 3 non-overlapping 11-mer siRNAs (Ambion Silencer® Select, Thermofisher) using Lipofectamine RNAiMax transfection reagent (Invitrogen ...
-
bioRxiv - Genomics 2023Quote: ... and transferred to a destination vector (pDEST_HC_Rec_Bxb_v2) compatible with recombination into the 293T LLP iCasp9 Blast cell line with a 1 h LR reaction (Thermo Fisher Scientific, 11-791-020). To generate a clone containing the full 5′ UTR exon 2 ...
-
bioRxiv - Microbiology 2024Quote: ... BMDMs were plated a day in advance on a coverslip lined 24-well plate at 8 x105 cells in 1mL per well, in “BMDM media” supplemented with 10% L929 (20% HI FBS, 1% penicillin-streptomycin, 1% non-essential amino acids (ThermoFisher, cat#11-140-050), 1% sodium pyruvate) ...
-
bioRxiv - Microbiology 2024Quote: ... and 1.5 µL of nuclease-free water were all added to 11 µL of viral RNA fraction and incubated in a MiniAmp thermal cycler (Applied Biosystems, Waltham, MA, USA) for 5.5 minutes at 89 °C ...
-
bioRxiv - Biochemistry 2022Quote: HEK293 wild type (ATCC, #CRL-1573) and HEK293 Flp-in T-REx (ThermoFisher, #R78007) wild type cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM ...
-
bioRxiv - Molecular Biology 2024Quote: HEK293 Flp-In T-REx cells (or HEK293 T-REx; Thermo Fisher Scientific, R78007) and HEK293 cells [American Type Culture Collection (ATCC) ...
-
bioRxiv - Cancer Biology 2021Quote: HEK293-FT (Thermo Fisher Scientific) were cultured in DMEM supplemented with 10% FBS ...
-
bioRxiv - Bioengineering 2021Quote: ... HEK293 Freestyle suspension cells (ThermoFisher), and immortalized human cord blood-derived mesenchymal stromal cells (cbMSCs ...
-
bioRxiv - Neuroscience 2020Quote: HEK293-FT (RRID:CVCL_6911, Thermo Scientific) cells were grown in DMEM containing 10% FBS ...
-
bioRxiv - Molecular Biology 2022Quote: HEK293-FlpIn cells (ThermoFisher Scientific) were transfected with empty vector (negative control ...
-
bioRxiv - Biochemistry 2022Quote: HEK293 FlpIn-TRex cells (Invitrogen) were cultured in DMEM (Gibco ...
-
bioRxiv - Cell Biology 2020Quote: HEK293 FlpIn-TRex cells (Invitrogen) were cultured in DMEM (Gibco ...
-
bioRxiv - Cancer Biology 2020Quote: ... HEK293-FT (Thermo Fisher Scientific) were cultured in DMEM with 10% FBS ...
-
bioRxiv - Cell Biology 2022Quote: ... HEK293-FT (Thermo Fisher Scientific) in DMEM with 10% FBS ...
-
bioRxiv - Molecular Biology 2023Quote: ... HEK293-F cells (ThermoFisher R79007) were transfected with the transfer plasmid and the packaging plasmids (Addgene plasmids #12263 and #8454 ...
-
bioRxiv - Molecular Biology 2019Quote: ... The knottin Fc fusion proteins were expressed in human embryonic kidney (HEK293) cells following the manufacturer’s protocols in the FreeStyle MAX 293 Expression System (Invitrogen). Secreted knottin-Fc fusion proteins were purified using Protein A Sepharose (Sigma ...
-
bioRxiv - Microbiology 2021Quote: ... Human parental HEK293 cells (ATCC, CRL-1573) were cultured in DMEM supplemented with 10% FBS and antibiotics/antimycotics (Gibco).
-
bioRxiv - Biochemistry 2023Quote: Human embryonic kidney (HEK293) and A549 cell lines were maintained in Dulbecco’s Modified Eagle Medium (Thermo Fisher Scientific, Lithuania) containing 10% heat-inactivated fetal bovine serum (Thermo Fisher Scientific) ...
-
bioRxiv - Bioengineering 2023Quote: ... Protein expression was achieved by transient transfection of FreeStyle human embryonic kidney 293 (HEK293-F) cells (Thermo Fisher Scientific). Cells were grown in non-baffled shake flasks (Corning ...
-
bioRxiv - Biophysics 2021Quote: ... The other end was ligated to a ∼190 bp biotin-labeled fragments created similarly by ApaI restriction of a 375 bp PCR amplicon of plasmid pBluKSP containing biotin-11-dUTP (Invitrogen, Life Technologies, Grand Island, NY, USA). All fragments were purified with PCR purification kits (Qiagen ...
-
bioRxiv - Neuroscience 2021Quote: ... the cell media was replaced with standard complete qNSC media with glucose or modified to have no glucose (Neurobasal A media Thermo Scientific, A2477501, no D-glucose, no sodium pyruvate, supplemented with 1x sodium pyruvate, Fisher Scientific, 11-360-070) for 48 hours ...
-
bioRxiv - Cancer Biology 2022Quote: ... #ACC 608) and CCRF-CEM (ATCC, #CRM-CCL-119) cell lines were maintained according to standard procedures in RPMI-1640 (Thermo Fisher Scientific, #11-875-119) with 10% (or 20% for CA46 and SET-2 cells ...
-
bioRxiv - Genomics 2023Quote: ... 100,000 PC-9 cells were seeded into each well of a 12-well plate in 1 mL of RPMI 1640 + L-Glutamine media (GIBCO, Cat. No. 11-875-093). 0 ...
-
bioRxiv - Microbiology 2023Quote: ... The obtained DNA was stored at -20 °C until PCR analysis DNA was quantified using a DeNovix DS-11 Series (Thermo Fisher NanoDrop™ One Spectrophotometer). DNA was analyzed for one-step or real-time PCR (qPCR ...
-
bioRxiv - Bioengineering 2023Quote: ... The GDF-15 and GFP antibodies were biotinylated using an EZ-Link Micro NHS-PEG4-Biotinylation Kit from Thermo Fisher Scientific (21955 ...
-
bioRxiv - Immunology 2022Quote: ... HEK293 cells stably transfected with human CXCR4 (HEK293- CXCR4) were used at passage 5 and were cultivated in DMEM medium (Gibco), supplemented with 10% FCS and 1% penicillin/streptomycin (Gibco) ...
-
bioRxiv - Molecular Biology 2020Quote: The template RNA for cDNA synthesis was isolated from human HEK293 cell culture with TRIzol reagent (Thermo Fisher Scientific, USA), or FFPE samples ...
-
bioRxiv - Developmental Biology 2022Quote: Human HEK293 cells were maintained at 37°C in complete media consisting of DMEM with high glucose GlutaMAX Supplement (Gibco) and 10% Fetal Bovine Serum ...
-
bioRxiv - Molecular Biology 2023Quote: Human embryonic kidney (HEK293) cells (RIKEN Cell Bank, Japan) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, GIBCO, 11965-092) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Molecular Biology 2022Quote: ... zipper tag via a 5XGGS linker region was co-transfected with N-terminally His-tagged human Leptin in suspension HEK293 FreeStyle cells (ThermoFisher) in the presence of 20 μM kifunensine (Dextra ...
-
bioRxiv - Bioengineering 2024Quote: Human Embryonic Kidney (HEK293; ATCC Ref: CRL-1573) cells were cultured in Dulbecco’s Modified Eagle Medium + GlutaMAX (Gibco; 10569-010) supplemented with 10% fetal bovine serum (Biowest ...
-
bioRxiv - Bioengineering 2024Quote: Human Embryonic Kidney (HEK293; ATCC Ref: CRL-1573) cells were cultured in Dulbecco’s Modified Eagle Medium + GlutaMAX (Gibco; 10569-010) supplemented with 10% fetal bovine serum (Biowest ...
-
bioRxiv - Microbiology 2022Quote: ... we used the following antibodies: α-complement C3 (primary: mAb 11H9, ThermoFisher catalog no. MA1-40046; secondary: Mouse anti-Rat IgG2a, FITC, Thermofisher catalog no. 11-4817-82), α-surfactant protein D (primary ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... RPTEC (ATCC® PCS-400-010™) and HepG2 (ATCC® HB-8065™) cells were cultured in complete DMEM/F-12 (Gibco 11-039-02), RPTEC media (ATCC® PCS-400-030™ ...
-
bioRxiv - Genomics 2022Quote: ... CD45−/CD31− /Thy1.2hi+ fibroblasts were fractionated by FACs using the following antibodies: a 1:100 dilution of Anti-Mouse CD31 antibody conjugated to FITC (Thermo Fisher Scientific, Cat#: 11-0311-81), a 1:100 dilution of Anti-Mouse CD45 antibody conjugated to FITC (Thermo Fisher Scientific ...